Mumps class-specific immunoglobulins in radioimmunoassay and conventional serology.

Abstract:

:Antibodies against mumps virus have been studied by using immunoglobulin class-specific indicators labeled with (125)I in the radioimmunoassay (RIA) procedure. The immunoglobulins in paired acute and convalescent sera were allowed to react with mumps virus in a solid-phase RIA system. Class-specific immunoglobulin indicators (anti-immunoglobulin M [IgM] and anti-immunoglobulin G [IgG]) labeled with (125)I revealed that immunoglobulins of early antisera were preponderantly IgM, whereas immunoglobulins of late antisera were predominantly IgG. These indicators detected antibodies of the early (IgM) and late (IgG) phases of the immune response. These findings are consistent with the classical temporal order of appearance of 19s (IgM) and 7s (IgG) globulins. Specificity of these indicators for reacting with fractionated 7s and 19s globulins is also presented. Mumps virus RIA obtained with anti-IgG correlated well with conventional serological data obtained by neutralization and hemagglutination inhibition, but most strongly with complement-fixation data. In addition, antibody bound by solid phase was capable of distinguishing between related antigens of the myxovirus group.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Daugharty H,Warfield DT,Hemingway WD,Casey HL

doi

10.1128/IAI.7.3.380-385.1973

subject

Has Abstract

pub_date

1973-03-01 00:00:00

pages

380-5

issue

3

eissn

0019-9567

issn

1098-5522

journal_volume

7

pub_type

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