Lipopolysaccharide down regulates both scavenger receptor B1 and ATP binding cassette transporter A1 in RAW cells.


:Lipopolysaccharide (LPS) has recently been shown to facilitate macrophage foam cell formation and has been suggested to be a proatherogenic factor. The mechanism of LPS induced cholesterol accumulation, however, is unclear. In this report, using the macrophage-like RAW 264.7 cell line, we provide experimental evidence that LPS's proatherogenic effects may at least in part reflect altered cholesterol metabolism. Data presented demonstrate that in a dose-dependent manner, LPS is able to down regulate the mRNA expression of the two primary high-density lipoprotein (HDL) receptors, scavenger receptor B1 (SR-B1) and ATP binding cassette A1 (ABCA1), with a 50% inhibitory concentration of less than 0.2 ng/ml, as well as to decrease SR-B1 protein expression by 80%. We also found that LPS treatment resulted in a significant decrease (to 20% of the control level) of the specific (125)I-HDL binding as well as in 50% inhibition of the HDL-mediated cholesterol efflux compared to untreated cells. In addition, we compared the potencies of various modified LPS preparations and demonstrated that the phosphorylated lipid A portion of LPS, which is highly conserved among gram-negative microorganisms, including Chlamydia, is primarily responsible for the effects of LPS on SR-B1 and ABCA1 expression. Inhibitors of NF-kappaB activation were observed to efficiently block the suppressive effect of LPS on SR-B1 and ABCA1, suggesting a mechanism involving NF-kappaB. These data indicate that the LPS effects on cholesterol metabolism may contribute to the proatherogenic properties of LPS.


Infect Immun


Infection and immunity


Baranova I,Vishnyakova T,Bocharov A,Chen Z,Remaley AT,Stonik J,Eggerman TL,Patterson AP





Has Abstract


2002-06-01 00:00:00












  • Plasmid-associated cell surface charge and hydrophobicity of Yersinia enterocolitica.

    abstract::Virulent strains of Yersinia enterocolitica and their plasmidless, avirulent derivatives were examined for their cell surface properties. Increased surface charge and hydrophobicity of Y. enterocolitica were found to be associated with the possession of a 40- to 48-megadalton plasmid. These surface properties were exp...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Lachica RV,Zink DL

    更新日期:1984-05-01 00:00:00

  • Protective role of Bacillus anthracis exosporium in macrophage-mediated killing by nitric oxide.

    abstract::The ability of the endospore-forming, gram-positive bacterium Bacillus anthracis to survive in activated macrophages is key to its germination and survival. In a previous publication, we discovered that exposure of primary murine macrophages to B. anthracis endospores upregulated NOS 2 concomitant with an .NO-dependen...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Weaver J,Kang TJ,Raines KW,Cao GL,Hibbs S,Tsai P,Baillie L,Rosen GM,Cross AS

    更新日期:2007-08-01 00:00:00

  • Dynamics of Escherichia coli infection and meningitis in infant rats.

    abstract::Escherichia coli strains isolated from newborn infants were injected intraperitoneally into infant rats. Strains possessing the K1 capsular polysaccharide antigen were significantly more virulent than strains lacking this antigen. When 5-day-old animals were injected with 1.2 X 10(1) colony forming units of a K1 E. co...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Bortolussi R,Ferrieri P,Wannamaker LW

    更新日期:1978-11-01 00:00:00

  • Circulating gamma delta T cells in response to Salmonella enterica serovar enteritidis exposure in chickens.

    abstract::gammadelta T cells are considered crucial to the outcome of various infectious diseases. The present study was undertaken to characterize gammadelta (T-cell receptor 1(+) [TCR1(+)]) T cells phenotypically and functionally in avian immune response. Day-old chicks were orally immunized with Salmonella enterica serovar E...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Berndt A,Pieper J,Methner U

    更新日期:2006-07-01 00:00:00

  • Neutralization of lymphocyte immortalization by different strains of Epstein-Barr virus with a murine monoclonal antibody.

    abstract::A murine monoclonal antibody was raised against the B95-8 strain of Epstein-Barr virus (EBV), which was isolated from a case of mononucleosis after blood transfusion (Hoffman et al. Proc. Natl. Acad. Sci. U.S.A. 77:2979, 1980). We provide evidence that neutralization of immortalization by this monoclonal antibody is v...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Miller G,Heston L,Hoffman G

    更新日期:1982-09-01 00:00:00

  • The repeat-in-toxin family member TosA mediates adherence of uropathogenic Escherichia coli and survival during bacteremia.

    abstract::Uropathogenic Escherichia coli (UPEC) is responsible for the majority of uncomplicated urinary tract infections (UTI) and represents the most common bacterial infection in adults. UPEC utilizes a wide range of virulence factors to colonize the host, including the novel repeat-in-toxin (RTX) protein TosA, which is spec...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Vigil PD,Wiles TJ,Engstrom MD,Prasov L,Mulvey MA,Mobley HL

    更新日期:2012-02-01 00:00:00

  • A heptosyltransferase mutant of Pasteurella multocida produces a truncated lipopolysaccharide structure and is attenuated in virulence.

    abstract::Pasteurella multocida is the causative agent of fowl cholera in birds. In a previous study using signature-tagged mutagenesis, we identified a mutant, AL251, which was attenuated for virulence in mice and in the natural chicken host. Sequence analysis indicated that AL251 had an insertional inactivation of the gene wa...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Harper M,Cox AD,St Michael F,Wilkie IW,Boyce JD,Adler B

    更新日期:2004-06-01 00:00:00

  • Delayed expulsion of adult Trichinella spiralis by mast cell-deficient W/Wv mice.

    abstract::Mast cell-deficient W/Wv mice and their mast cell-sufficient littermates were given infections of Trichinella spiralis. W/Wv mice were slower than their littermates to expel adult T. spiralis. Repair of the mast cell deficiency of W/Wv mice by bone marrow grafting was accompanied by accelerated expulsion of T. spirali...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Ha TY,Reed ND,Crowle PK

    更新日期:1983-07-01 00:00:00

  • Pseudomonas aeruginosa clearance in mice: comparison of tissue, strain, and corticosteroid effects.

    abstract::We used a murine model to determine whether chronic corticosteroid therapy has uniform effects on bacterial clearance processes in different tissues. After a 2-week regimen of oral prednisolone, Swiss Webster mice were challenged with Pseudomonas aeruginosa strain PAO1 or virulent derivatives of PAO1 (IP-8 and IT-10)....

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Nugent KM,Cox CD,Pesanti EL

    更新日期:1984-03-01 00:00:00

  • Clonal polymorphism of Borrelia burgdorferi strain B31 MI: implications for mutagenesis in an infectious strain background.

    abstract::A major obstacle to studying the functions of particular gene products in the mouse-tick infectious cycle of Borrelia burgdorferi has been an inability to knock out genes in pathogenic strains. Here, we investigated conditions for site-directed mutagenesis in B31 MI, the low-passage-number, infectious B. burgdorferi s...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Elias AF,Stewart PE,Grimm D,Caimano MJ,Eggers CH,Tilly K,Bono JL,Akins DR,Radolf JD,Schwan TG,Rosa P

    更新日期:2002-04-01 00:00:00

  • Expression of cathelicidin LL-37 during Mycobacterium tuberculosis infection in human alveolar macrophages, monocytes, neutrophils, and epithelial cells.

    abstract::The innate immune response in human tuberculosis is not completely understood. To improve our knowledge regarding the role of cathelicidin hCAP-18/LL37 in the innate immune response to tuberculosis infection, we used immunohistochemistry, immunoelectron microscopy, and gene expression to study the induction and produc...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Rivas-Santiago B,Hernandez-Pando R,Carranza C,Juarez E,Contreras JL,Aguilar-Leon D,Torres M,Sada E

    更新日期:2008-03-01 00:00:00

  • Expression of the cloned toxic shock syndrome toxin 1 gene (tst) in vivo with a rabbit uterine model.

    abstract::Toxic shock syndrome (TSS) toxin 1 (TSST1) is produced by strains of Staphylococcus aureus associated with TSS. Purified TSST1 induces in rabbits a shock-like illness with many features similar to TSS in humans. These symptoms were also induced by TSST1-producing bacteria in diffusion chambers implanted in the rabbit ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: de Azavedo JC,Foster TJ,Hartigan PJ,Arbuthnott JP,O'Reilly M,Kreiswirth BN,Novick RP

    更新日期:1985-10-01 00:00:00

  • Experimental murine candidiasis: cell-mediated immunity after cutaneous challenge.

    abstract::Attempts were made to isolate an antigen(s) from Candida albicans suitable for detecting hypersensitivity in a murine model of candidiasis. Using footpad reactivity in cutaneously infected animals as the assay, comparisons were made of two commercial extracts and cell wall and cytoplasmic preparations made in the labo...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Domer JE,Moser SA

    更新日期:1978-04-01 00:00:00

  • Temporal quorum-sensing induction regulates Vibrio cholerae biofilm architecture.

    abstract::Vibrio cholerae, the pathogen that causes cholera, also survives in aqueous reservoirs, probably in the form of biofilms. Quorum sensing negatively regulates V. cholerae biofilm formation through HapR, whose expression is induced at a high cell density. In this study, we show that the concentration of the quorum-sensi...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Liu Z,Stirling FR,Zhu J

    更新日期:2007-01-01 00:00:00

  • Differential effects of the streptococcal fibronectin-binding protein, FBP54, on adhesion of group A streptococci to human buccal cells and HEp-2 tissue culture cells.

    abstract::We have previously demonstrated that fibronectin mediates streptococcal adhesion to host cells and that streptococci interact primarily with the N-terminal domain of fibronectin. FBP54 is a 54-kDa protein from group A streptococci that binds fibronectin. In this report, we show that the N-terminal domain of fibronecti...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Courtney HS,Dale JB,Hasty DI

    更新日期:1996-07-01 00:00:00

  • Passive hemolysis test for antibody to Treponema hyodysenteriae.

    abstract::Swine were infected orally with pure cultures of Treponema hyodysenteriae, and the humoral antibody response was measured by a passive hemolysis test (passive hemagglutination test with the use of complement). Antibody to T. hyodysenteriae was detected as early as 1 week and later on at 4 months after exposure. Howeve...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Jenkins EM,Sinha PP,Vance RT,Reese GL

    更新日期:1976-10-01 00:00:00

  • Toll-like receptors 2 and 4 contribute to sepsis-induced depletion of spleen dendritic cells.

    abstract::Depletion of dendritic cells (DC) in secondary lymphoid organs is a hallmark of sepsis-induced immune dysfunction. In this setting, we investigated if Toll-like receptor (TLR)-dependent signaling might modulate the maturation process and the survival of DC. Using a model of sublethal polymicrobial sepsis induced by ce...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Pène F,Courtine E,Ouaaz F,Zuber B,Sauneuf B,Sirgo G,Rousseau C,Toubiana J,Balloy V,Chignard M,Mira JP,Chiche JD

    更新日期:2009-12-01 00:00:00

  • Cell surface-exposed tetanus toxin fragment C produced by recombinant Bacillus anthracis protects against tetanus toxin.

    abstract::Bacillus anthracis, the causal agent of anthrax, synthesizes two surface layer (S-layer) proteins, EA1 and Sap, which account for 5 to 10% of total protein and are expressed in vivo. A recombinant B. anthracis strain was constructed by integrating into the chromosome a translational fusion harboring the DNA fragments ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Mesnage S,Weber-Levy M,Haustant M,Mock M,Fouet A

    更新日期:1999-09-01 00:00:00

  • Pathogenicity of human and porcine intestinal spirochetes in one-day-old specific-pathogen-free chicks: an animal model of intestinal spirochetosis.

    abstract::One-day-old chicks were infected orally with two strains of weakly hemolytic spirochetes isolated from a human and a pig with intestinal spirochetosis. These spirochetosis both colonized birds, attached end-on to their cecal enterocytes, induced watery diarrhea, and significantly depressed growth rates. Cultures of Se...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Trott DJ,McLaren AJ,Hampson DJ

    更新日期:1995-09-01 00:00:00

  • Genetic characterization of DNA region containing the trh and ure genes of Vibrio parahaemolyticus.

    abstract::We have demonstrated that possession of the gene for thermostable direct hemolysin-related hemolysin (trh) coincides with the presence of the urease gene among clinical Vibrio parahaemolyticus strains and that the location of the two genes are in close proximity on the chromosome. Here, we cloned and sequenced the 15,...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Park KS,Iida T,Yamaichi Y,Oyagi T,Yamamoto K,Honda T

    更新日期:2000-10-01 00:00:00

  • Control of Mucosal Candidiasis in the Zebrafish Swim Bladder Depends on Neutrophils That Block Filament Invasion and Drive Extracellular-Trap Production.

    abstract::Candida albicans is a ubiquitous mucosal commensal that is normally prevented from causing acute or chronic invasive disease. Neutrophils contribute to protection in oral infection but exacerbate vulvovaginal candidiasis. To dissect the role of neutrophils during mucosal candidiasis, we took advantage of a new, transp...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Gratacap RL,Scherer AK,Seman BG,Wheeler RT

    更新日期:2017-08-18 00:00:00

  • Comparison of plasmid deoxyribonucleic acid contents, culture characteristics, and indices of pathogenicity among selected strains of Vibrio parahaemolyticus.

    abstract::Thirty-one Kanagawa-positive and -negative Vibrio parahaemolyticus isolates from patient stools and seafood were examined for plasmid deoxyribonucleic acid content, culture characteristics, and indices of pathogenicity. No significant correlation was found between plasmid deoxyribonucleic acid contents and indices of ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Twedt RM,Brown DF,Zink DL

    更新日期:1981-07-01 00:00:00

  • Critical role of neutrophils in eliminating Listeria monocytogenes from the central nervous system during experimental murine listeriosis.

    abstract::Neutrophils are the main inflammatory cell present in lesions involving the central nervous system (CNS) during human and murine listeriosis. In this study, administration of the neutrophil-depleting monoclonal antibody RB6-8C5 during experimental murine listeriosis facilitated the multiplication of Listeria monocytog...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: López S,Marco AJ,Prats N,Czuprynski CJ

    更新日期:2000-08-01 00:00:00

  • Fusobacterium nucleatum increases collagenase 3 production and migration of epithelial cells.

    abstract::Fusobacterium nucleatum is closely associated with human periodontal diseases and may also be a causative agent in other infections, such as pericarditis, septic arthritis, and abscesses of tonsils and liver. Initiation and outcome of infective diseases depend critically on the host cell signaling system altered by th...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Uitto VJ,Baillie D,Wu Q,Gendron R,Grenier D,Putnins EE,Kanervo A,Firth JD

    更新日期:2005-02-01 00:00:00

  • Toll-like receptor 6 plays an important role in host innate resistance to Brucella abortus infection in mice.

    abstract::Brucella abortus is recognized by several Toll-like receptor (TLR)-associated pathways triggering proinflammatory responses that affect both the nature and intensity of the immune response. Previously, we demonstrated that B. abortus-mediated dendritic cell (DC) maturation and control of infection are dependent on the...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: de Almeida LA,Macedo GC,Marinho FA,Gomes MT,Corsetti PP,Silva AM,Cassataro J,Giambartolomei GH,Oliveira SC

    更新日期:2013-05-01 00:00:00

  • Human monocytes promote Th1 and Th17 responses to Streptococcus pneumoniae.

    abstract::Streptococcus pneumoniae is a leading cause of bacterial pneumonia, meningitis, and sepsis in children. Human immunity to pneumococcal infections has been assumed to depend on anticapsular antibodies. However, recent findings from murine models suggest that alternative mechanisms, dependent on T helper cells, are also...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Olliver M,Hiew J,Mellroth P,Henriques-Normark B,Bergman P

    更新日期:2011-10-01 00:00:00

  • Isolation of Bacteroides fragilis mutants with in vivo growth defects by using Tn4400', a modified Tn4400 transposition system, and a new screening method.

    abstract::A modified version of the Bacteroides fragilis transposon Tn4400, designated Tn4400', enabling rapid isolation and analysis of B. fragilis mutants has been constructed. To identify potential virulence factors, Tn4400'-generated mutants were screened by a new method; this resulted in the isolation of 21 mutant strains ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Tang YP,Malamy MH

    更新日期:2000-01-01 00:00:00

  • Identification and characterization of a novel family of pneumococcal proteins that are protective against sepsis.

    abstract::Four pneumococcal genes (phtA, phtB, phtD, and phtE) encoding a novel family of homologous proteins (32 to 87% identity) were identified from the Streptococcus pneumoniae genomic sequence. These open reading frames were selected as potential vaccine candidates based upon their possession of hydrophobic leader sequence...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Adamou JE,Heinrichs JH,Erwin AL,Walsh W,Gayle T,Dormitzer M,Dagan R,Brewah YA,Barren P,Lathigra R,Langermann S,Koenig S,Johnson S

    更新日期:2001-02-01 00:00:00

  • Immunization with a polyprotein vaccine consisting of the T-Cell antigens thiol-specific antioxidant, Leishmania major stress-inducible protein 1, and Leishmania elongation initiation factor protects against leishmaniasis.

    abstract::Development of an effective vaccine against Leishmania infection is a priority of tropical disease research. We have recently demonstrated protection against Leishmania major in the murine and nonhuman primate models with individual or combinations of purified leishmanial recombinant antigens delivered as plasmid DNA ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Coler RN,Skeiky YA,Bernards K,Greeson K,Carter D,Cornellison CD,Modabber F,Campos-Neto A,Reed SG

    更新日期:2002-08-01 00:00:00

  • Release of soluble peptidoglycan from growing gonococci: hexaminidase and amidase activities.

    abstract::Peptidoglycan (PG) turnover in exponentially growing Neisseria gonorrhoeae RD5 type 4 was accompanied by release of soluble PG fragments into the medium. Turnover of the D-[14C]glucosamine-labeled glycan moiety and of the meso-[3H]diaminopimelic acid (DAP)-labeled peptide region occurred at similar rates (ca. 35% per ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Rosenthal RS

    更新日期:1979-06-01 00:00:00