Abstract:
:In mammals, the natural resistance-associated macrophage protein 1 gene, Nramp1, plays a major role in resistance to mycobacterial infections. Chesapeake Bay striped bass (Morone saxatilis) is currently experiencing an epizootic of mycobacteriosis that threatens the health of this ecologically and economically important species. In the present study, we characterized an Nramp gene in this species and obtained evidence that there is induction following Mycobacterium exposure. The striped bass Nramp gene (MsNramp) and a 554-amino-acid sequence contain all the signal features of the Nramp family, including a topology of 12 transmembrane domains (TM), the transport protein-specific binding-protein-dependent transport system inner membrane component signature, three N-linked glycosylation sites between TM 7 and TM 8, sites of casein kinase and protein kinase C phosphorylation in the amino and carboxy termini, and a tyrosine kinase phosphorylation site between TM 6 and TM 7. Phylogenetic analysis most closely grouped MsNramp with other teleost Nramp genes and revealed high sequence similarity with mammalian Nramp2. MsNramp expression was present in all tissues assayed by reverse transcription-PCR. Within 1 day of injection of Mycobacterium marinum, MsNramp expression was highly induced (17-fold higher) in peritoneal exudate (PE) cells compared to the expression in controls. The levels of MsNramp were three- and sixfold higher on days 3 and 15, respectively. Injection of Mycobacterium shottsii resulted in two-, five-, and threefold increases in gene expression in PE cells over the time course. This report is the first report of induction of an Nramp gene by mycobacteria in a poikilothermic vertebrate.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Burge EJ,Gauthier DT,Ottinger CA,Van Veld PAdoi
10.1128/iai.72.3.1626-1636.2004keywords:
subject
Has Abstractpub_date
2004-03-01 00:00:00pages
1626-36issue
3eissn
0019-9567issn
1098-5522journal_volume
72pub_type
杂志文章abstract::The mechanism by which capsular polysaccharides inhibit phagocytosis is not clearly understood. We investigated the association between a negative surface charge and inhibition of phagocytosis by the capsular polysaccharide of Cryptococcus neoformans. A two-polymer aqueous-phase system containing phosphate ions was us...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-08-01 00:00:00
abstract::We have previously shown that infection by Trypanosoma cruzi, a parasitic protozoan, is reduced by injection of CD40 ligand (CD40L)-transfected 3T3 fibroblasts (D. Chaussabel, F. Jacobs, J. de Jonge, M. de Veerman, Y. Carlier, K. Thielemans, M. Goldman, and B. Vray, Infect. Immun. 67:1929-1934, 1999). This prompted us...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.10.6552-6561.2005
更新日期:2005-10-01 00:00:00
abstract::Lipopolysaccharide (LPS) has recently been shown to facilitate macrophage foam cell formation and has been suggested to be a proatherogenic factor. The mechanism of LPS induced cholesterol accumulation, however, is unclear. In this report, using the macrophage-like RAW 264.7 cell line, we provide experimental evidence...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.6.2995-3003.2002
更新日期:2002-06-01 00:00:00
abstract::Tubercle bacilli failed to grow in iron-void media enriched with solutions of iron-containing transferrin (Tr) or ferritin (F) because these substances do not provide the bacilli with iron, which is essential for their growth. Animal serum and macrophages possessed no iron carrier with an ability to satisfy the need o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.1.34-40.1974
更新日期:1974-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.5.1.91-97.1972
更新日期:1972-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.1.457-459.1991
更新日期:1991-01-01 00:00:00
abstract::Streptococcus pneumoniae (the pneumococcus), a leading cause of bacterial disease, is most commonly carried in the human nasopharynx. Colonization induces inflammation that promotes the organism's growth and transmission. This inflammatory response is dependent on intracellular sensing of bacterial components that acc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00224-15
更新日期:2015-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.5.1916-1925.1997
更新日期:1997-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05904-11
更新日期:2012-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.12.4943-4950.1997
更新日期:1997-12-01 00:00:00
abstract::We have previously demonstrated that protection from allergic inflammation by Ascaris suum infection was characterized by a global increase in interleukin-10 (IL-10) and the development of protective CD4(+)/CD25(+) T cells (L. Schopf, S. Luccioli, V. Bundoc, P. Justice, C. C. Chan, B. J. Wetzel, H. H. Norris, J. F. Ur...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00720-06
更新日期:2006-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00246-20
更新日期:2020-10-19 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02617-14
更新日期:2015-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.3.1098-1108.2003
更新日期:2003-03-01 00:00:00
abstract::Increased incidence of bovine tuberculosis (TB) in the United Kingdom caused by infection with Mycobacterium bovis is a cause of considerable economic loss to farmers and the government. The Eurasian badger (Meles meles) represents a wildlife source of recurrent M. bovis infections of cattle in the United Kingdom, and...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00052-08
更新日期:2008-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.11.6307-6319.2003
更新日期:2003-11-01 00:00:00
abstract::Capsular glucuronoxylomannans (GXM) of Cryptococcus neoformans var. neoformans isolates from patients with recurrent cryptococcal meningitis were analyzed by 1H nuclear magnetic resonance spectroscopy and for reactivity with factor sera (Iatron, Tokyo, Japan). For each patient the initial and relapse isolates had prev...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.5.1899-1905.1995
更新日期:1995-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.11.5183-5189.1998
更新日期:1998-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.5.1069-1078.1975
更新日期:1975-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.2.1436-1441.2006
更新日期:2006-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.4.635-639.1975
更新日期:1975-04-01 00:00:00
abstract::Borrelia burgdorferi, the causative agent of Lyme arthritis, does not produce any exported proteases capable of degrading extracellular matrix despite the fact that it is able to disseminate from a skin insertion site to infect multiple organs. Prior studies have shown that B. burgdorferi induces the host protease, ma...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00214-09
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.33.2.485-490.1981
更新日期:1981-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.11.2918-2924.1988
更新日期:1988-11-01 00:00:00
abstract::An immunomodulatory role of arthropod saliva has been well documented, but evidence for an effect on Plasmodium sp. infectiousness remains controversial. Mosquito saliva may orient the immune response toward a Th2 profile, thereby priming a Th2 response against subsequent antigens, including Plasmodium. Orientation to...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.06414-11
更新日期:2012-06-01 00:00:00
abstract::Trypanosoma musculi produces a chronic infection with a consistently elevated parasitemia in nude mice. Thymic reconstitution of nude mice restores immunity to the infection. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.2.715-716.1977
更新日期:1977-05-01 00:00:00
abstract::TB10.4 is a newly identified antigen of Mycobacterium tuberculosis recognized by human and murine T cells upon mycobacterial infection. Here, we show that immunization with Mycobacterium bovis BCG induces a strong, genetically controlled, Th1 immune response against TB10.4 in mice. BALB/c and C57BL/6 strains behave as...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02086-05
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.8.4027-4032.1999
更新日期:1999-08-01 00:00:00
abstract::The coaggregation-defective (COG-) mutant Actinomyces viscosus T14V(PK455), which is unable to participate in lactose-sensitive adherence, and its COG+ parent were compared to structurally define the mutational loss of cell-associated lectin activity. Immunoelectrophoretic comparisons of crude extracts or isolated fim...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.2.759-765.1983
更新日期:1983-05-01 00:00:00
abstract::Streptococcus suis is one of the most important pathogens affecting the swine industry and is also an emerging zoonotic agent for humans. Two-component signaling systems (TCSs) play important roles in the adaptation of pathogenic bacteria to host environments. In this study, we identified a novel TCS, named TCS09HKRR,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00377-19
更新日期:2019-08-21 00:00:00