Abstract:
:The coaggregation-defective (COG-) mutant Actinomyces viscosus T14V(PK455), which is unable to participate in lactose-sensitive adherence, and its COG+ parent were compared to structurally define the mutational loss of cell-associated lectin activity. Immunoelectrophoretic comparisons of crude extracts or isolated fimbriae from both strains demonstrated type 2 fimbriae (previously designated Ag2) in preparations obtained from the parent but none in those obtained from the mutant. This result was verified by the immunoelectron-microscopic identification of type 1 (previously designated Ag1 or VAl) and type 2 fimbriae on the parent organism but only type 1 fimbriae on the mutant. A comparison of the amounts of extractable fimbriae of each type and the capacity of the cells to bind 14C-labeled monoclonal antibodies specific for each fimbrial component showed that the mutational loss of type 2 fimbriae had no significant quantitative effect on fimbriation of the COG- mutant with type 1 structures. Cells of A. viscosus T14AV, a mutant with various adherence defects that include the COG- phenotype, displayed fimbriae of both types, but in greatly reduced amounts. Thus, the properties of mutant strain T14V(PK455) associated the lectin activity with type 2 fimbriae, whereas those of strain T14AV provided little insight into the mechanism of lactose-sensitive adherence. In addition, the precise nature of the cell surface modification displayed by strain T14V(PK455) provides clear evidence for the existence of distinct and independent fimbriae on A. viscosus T14V.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Cisar JO,Curl SH,Kolenbrander PE,Vatter AEdoi
10.1128/IAI.40.2.759-765.1983subject
Has Abstractpub_date
1983-05-01 00:00:00pages
759-65issue
2eissn
0019-9567issn
1098-5522journal_volume
40pub_type
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doi:10.1128/IAI.63.8.3054-3061.1995
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更新日期:1998-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1991-08-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1977-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.48.1.119-123.1985
更新日期:1985-04-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.42.2.501-509.1983
更新日期:1983-11-01 00:00:00
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更新日期:1993-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/IAI.69.11.6951-6961.2001
更新日期:2001-11-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.1.41-47.1989
更新日期:1989-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2004-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.1.269-276.1996
更新日期:1996-01-01 00:00:00
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journal_title:Infection and immunity
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pub_type: 杂志文章
doi:10.1128/iai.71.6.3361-3370.2003
更新日期:2003-06-01 00:00:00
abstract::Expression of virulence determinants by Bordetella pertussis, the primary etiological agent of whooping cough, is regulated by the BvgAS two-component regulatory system. The role of a second two-component regulatory system, encoded by risAS, in this process is not defined. Here, we show that mutation of B. pertussis r...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.9.5995-6004.2005
更新日期:2005-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.12.5421-5424.1996
更新日期:1996-12-01 00:00:00
abstract::Conidia ingested by resident macrophages had an enhanced percentage of transformation to yeast cells compared with those in culture medium without macrophages. The yeast cells subsequently grew intracellularly by budding. Macrophages treated with cytokines from antigen-stimulated spleen cells from immunized mice signi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.5.2096-2100.1992
更新日期:1992-05-01 00:00:00