Abstract:
:The effects of an ornithine-containing lipid [alpha-N-(3-acyloxyacyl)-ornithine (Orn-L)] or a serine-containing lipid [alpha-N-(3-acyloxyacyl)-serine (Ser-L)] from Flavobacterium meningosepticum on lethal endotoxemia in mice were examined. When 500 micrograms of Orn-L was intravenously administered 1 h before intravenous administration of a lethal dose of endotoxin, none of the mice died. The protective effect of Ser-L was weaker than that of Orn-L. Light and electron microscopic studies demonstrated that necrosis of hepatocytes caused by endotoxin was prevented by pretreatment with Orn-L. Furthermore, Kupffer cells were activated morphologically 1 h after the administration of Orn-L or Ser-L, and the liposomes of the lipoamino acids were incorporated into phagolysosomes in activated Kupffer cells. The activity of tumor necrosis factor in sera of endotoxin-treated mice was decreased markedly by pretreatment of mice with Orn-L. In vitro, the lipoamino acids suppressed endotoxin-induced tumor necrosis factor generation but did not suppress tumor necrosis factor generation induced by zymosan and whole cells of Staphylococcus aureus. These results suggested that Orn-L and Ser-L can be used as specific blocking agents against endotoxin. The blocking mechanism may be antagonistic, because of the structural similarities between the lipoamino acids and endotoxin lipid A.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Kawai Y,Kaneda K,Morisawa Y,Akagawa Kdoi
10.1128/IAI.59.8.2560-2566.1991subject
Has Abstractpub_date
1991-08-01 00:00:00pages
2560-6issue
8eissn
0019-9567issn
1098-5522journal_volume
59pub_type
杂志文章abstract::In order to evaluate during experimental Trypanosoma brucei infections the potential role of tumor necrosis factor alpha (TNF-alpha) in the host-parasite interrelationship, C57BL/6 TNF-alpha knockout mice (TNF-alpha-/-) as well as C57BL/6 wild-type mice were infected with pleomorphic T. brucei AnTat 1.1 E parasites. I...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.6.3128-3132.1999
更新日期:1999-06-01 00:00:00
abstract::Tuberculous pleurisy allows the study of human cells at the site of active Mycobacterium tuberculosis infection. In this study, we found that among pleural fluid (PF) lymphocytes, natural killer (NK) cells are a major source of early gamma interferon (IFN-gamma) upon M. tuberculosis stimulation, leading us to investig...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00381-07
更新日期:2007-11-01 00:00:00
abstract::The immunogenicity of a single dose of a whole-cell vaccine of inactivated Borrelia burgdorferi HSF (human spinal fluid isolate) was evaluated. Syrian hamsters were vaccinated subcutaneously and challenged by the intraperitoneal injection of 1,000 50% infectious doses of B. burgdorferi HSF 30 or 90 days postvaccinatio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.3.897-898.1986
更新日期:1986-12-01 00:00:00
abstract::Pseudomonas aeruginosa is a gram-negative bacillus that is a major cause of morbidity and mortality in immunosuppressed patients, burn patients, and patients with cystic fibrosis. Although immunity to these bacteria has been associated with serum antibody, more recent evidence suggests that T-cell-mediated immunity ma...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.2.670-674.1983
更新日期:1983-05-01 00:00:00
abstract::For the first time, hepatitis A viral antigen (HAAg) was shown in liver biopsy tissue from a patient in the acute phase of hepatitis type A by light and electron microscopy, using the peroxidase-antibody technique. Under light microscopy, the staining for HAAg appeared as a fine, granular reaction product, scattered t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.36.1.320-324.1982
更新日期:1982-04-01 00:00:00
abstract::Staphylococcus aureus nasal carriage is a risk factor for infection in humans, particularly in the hospital environment. Attenuation of carriage has proven effective in reducing the prevalence of infection in some high-risk groups. To study staphylococcal factors that influence nasal colonization, a mouse model of S. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.10.5001-5006.1999
更新日期:1999-10-01 00:00:00
abstract::Numerous bacterial pathogens use type III secretion systems (T3SSs) or T4SSs to inject or translocate virulence proteins into eukaryotic cells. Several different reporter systems have been developed to measure the translocation of these proteins. In this study, a peptide tag-based reporter system was developed and use...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00690-06
更新日期:2006-10-01 00:00:00
abstract::Dried and wet mouse fibroblast monolayers with labeled collagenous substrate were used to study the effects of lipoteichoic acid (LTA) on cellular prolyl hydroxylase activity. LTA is a scavenger of cations, and Fe2+ is essential for prolyl hydroxylase activity. Surprisingly, addition of LTA to dried monolayers resulte...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.3.745-752.1985
更新日期:1985-12-01 00:00:00
abstract::Borrelia burgdorferi is a causative agent of Lyme disease, the most common arthropod-borne disease in the United States. B. burgdorferi evades host immune defenses to establish a persistent, disseminated infection. Previous work showed that P66-deficient B. burgdorferi (Δp66) is cleared quickly after inoculation in mi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00186-17
更新日期:2018-01-22 00:00:00
abstract::We demonstrate that a mucoid, alginate-producing strain of Pseudomonas aeruginosa isolated from the lungs of a cystic fibrosis (CF) patient secretes multiple enzymes with nucleoside diphosphate kinase (Ndk), ATPase, adenylate kinase, 5'-nucleotidase, and ATP-modifying enzymatic activities. The secretion is triggered a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.10.5231-5242.1999
更新日期:1999-10-01 00:00:00
abstract::Since the pH of the gingival crevice increases from below neutrality in health to above pH 8 in disease, we decided to investigate the effect of environmental pH on the growth and enzyme activity of Bacteroides gingivalis W50. Cells were grown in a chemostat under hemin-excess conditions over a range of pH values; sta...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.5.1096-1100.1988
更新日期:1988-05-01 00:00:00
abstract::Disruption of neutrophils causes the release of a cytoplasmic protein which can inhibit the growth of Candida albicans without killing the organisms. The present study was undertaken with a combined system in which candidacidal and candidastatic activities of human neutrophils could be compared. In this system it was ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.8.2696-2698.1990
更新日期:1990-08-01 00:00:00
abstract::The objective of this research was to determine whether gram-negative bacteria frequently isolated from periodontally diseased sites contained polyclonal B-cell activators. Polyclonal B-cell activation, which results in nonspecific activation of multiple B-cell clones was analyzed by a hemolysis-in-gel assay designed ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.1.43-49.1981
更新日期:1981-10-01 00:00:00
abstract::Staphylococcus aureus experimental endocarditis relies on sequential fibrinogen binding (for valve colonization) and fibronectin binding (for endothelial invasion) conferred by peptidoglycan-attached adhesins. Fibronectin-binding protein A (FnBPA) reconciles these two properties--as well as elastin binding--and promot...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00405-08
更新日期:2008-08-01 00:00:00
abstract::Most oral bacteria tested formed colonies on a chemically defined medium with a chromogenic arylsulfatase substrate. Arylsulfatase activity was, however, restricted to Campylobacter-Wolinella group organisms, including Wolinella recta, a possible periodontopathogen. W. recta was the only arylsulfatase-active species a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.5.1380-1383.1989
更新日期:1989-05-01 00:00:00
abstract::C57BL/6J mice infected with Plasmodium berghei ANKA develop neurological dysfunction and die within 7 days of infection. We show that treatment of infected mice with a kynurenine-3-hydroxylase inhibitor prevents them from developing neurological symptoms and extends their life span threefold until severe anemia develo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.8.5249-5251.2005
更新日期:2005-08-01 00:00:00
abstract::Levels of the serum opsonin mannan-binding lectin (MBL) were directly correlated with the probability of developing visceral leishmaniasis. Monocytes infected with MBL-opsonized Leishmania chagasi promastigotes secreted higher levels of tumor necrosis factor alpha and interleukin-6 than cells infected with nonopsonize...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.8.5212-5215.2001
更新日期:2001-08-01 00:00:00
abstract::We examined the association of Herpesvirus saimiri with lymphocyte subsets and the functional integrity and distribution of these populations in owl monkeys with chronic, disease-free infections, in uninoculated, control animals, and in one monkey with H. saimiri-induced lymphoma. The lymphocyte subpopulations examine...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.32.2.698-706.1981
更新日期:1981-05-01 00:00:00
abstract::We characterized outer membrane proteins (OMPs) from selected Campylobacter jejuni, C. coli, and C. fetus strains by two-dimensional gel electrophoresis (2DGE), using isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and by immunoblotting with immune rabbit serum. The flage...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1987-07-01 00:00:00
abstract::Heart and muscle tissues appear to be specific target organs for the in vivo inhibition of protein synthesis by diphtheria toxin. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.2.5.686-688.1970
更新日期:1970-11-01 00:00:00
abstract::Toxin A (TcdA) and toxin B (TcdB) are major virulence factors of Clostridium difficile. These two toxins intoxicate cultured cells by similar mechanisms, and TcdB generally is more potent than TcdA in cultured cells. The exact reason for this difference is unclear. Here, we report that the cellular effects of TcdA can...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01577-08
更新日期:2009-06-01 00:00:00
abstract::Once in the cytoplasm of mammalian cells, Shigella flexneri expresses a motile phenotype caused by polar directional assembly of actin. This process depends on accumulation of IcsA (VirG), a 120-kDa protein with ATPase activity, at the pole of the bacterium opposite to that at which ongoing septation occurs. IcsA is a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.2.511-517.1996
更新日期:1996-02-01 00:00:00
abstract::The comparative immunogenicity of the two cross-reacting group 6 pneumococcal capsular polysaccharides, type 6A(6) and type 6B(26), was studied with hyperimmune rabbit typing antisera and with sera from adult volunteers injected with polyvalent pneumococcal vaccines containing either 50 mug of type 6A (U.S. designatio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.3.1116-1122.1979
更新日期:1979-12-01 00:00:00
abstract::Ribosomes of strain NOR-7 of group B Neisseria meningitidis were isolated by a procedure that included treatment of the cells with sodium dodecyl sulfate, disruption in a French pressure cell, and differential centrifugation. These preparations consisted of 66% ribonucleic acid and 24% protein and sedimented as a sing...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.6.3.355-363.1972
更新日期:1972-09-01 00:00:00
abstract::Hydrogen sulfide (H(2)S) is a major metabolic end product detected in deep periodontal pockets that is produced by resident periodontopathic microbiota associated with the progression of periodontitis. Treponema denticola, a member of the subgingival biofilm at disease sites, produces cystalysin, an enzyme that catabo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.3.1113-1120.2002
更新日期:2002-03-01 00:00:00
abstract::Neisseria meningitidis crosses the blood-brain barrier (BBB) following the activation of the β2-adrenergic receptor by the type IV pili (TFP). Two components of the type IV pili recruit the β2-adrenergic receptor, the major pilin PilE and the minor pilin PilV. Here, we report that a strain deleted of PilX, one of the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00369-12
更新日期:2012-09-01 00:00:00
abstract::Several previous findings have suggested that the cationic nature of lysozyme is a major factor in its bactericidal activity. Since a number of cationic proteins or peptides have been reported to cause membrane damage in bacteria, we investigated the effect of lysozyme on glucose fermentation and intracellular pH and ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.2.638-644.1991
更新日期:1991-02-01 00:00:00
abstract::Modification of three or four of the five histidine residues in the toxic shock syndrome toxin 1 (TSST-1) with diethylpyrocarbonate did not inhibit the precipitin reaction of the modified TSST-1 with polyvalent antisera to the toxin. Monoclonal antibody 7T did not react with the modified TSST-1, but monoclonal antibod...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.7.1901-1905.1989
更新日期:1989-07-01 00:00:00
abstract::Chronic lung infections with Pseudomonas aeruginosa biofilms are associated with refractory and fatal pneumonia in cystic fibrosis (CF). In this study, a group of genomically diverse P. aeruginosa isolates were compared with the reference strain PAO1 to assess the roles of motility, twitching, growth rate, and overpro...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.1.133-144.2004
更新日期:2004-01-01 00:00:00
abstract::Purified cell walls representing a wide variety in teichoic acid and peptidoglycan structure prepared from eight different gram-positive bacterial species induced the production of tumor necrosis factor alpha (TNF-alpha) and interleukin-6 from human monocytes in the presence of 10% plasma or serum. Significant amounts...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.7.2715-2721.1994
更新日期:1994-07-01 00:00:00