Abstract:
:Neisseria meningitidis crosses the blood-brain barrier (BBB) following the activation of the β2-adrenergic receptor by the type IV pili (TFP). Two components of the type IV pili recruit the β2-adrenergic receptor, the major pilin PilE and the minor pilin PilV. Here, we report that a strain deleted of PilX, one of the three minor pilins, is defective in endothelial cell signaling. The signaling role of PilX was abolished when pili were not retractable. Purified PilX was unable to recruit the β2-adrenergic receptor, thus suggesting that PilX was playing an indirect role in endothelial cell signaling. Considering the recent finding that type IV pili can transition into a new conformation (N. Biais, D. L. Higashi, J. Brujic, M. So, and M. P. Sheetz, Proc. Natl. Acad. Sci. U. S. A. 107:11358-11363, 2010), we hypothesized that PilX was responsible for a structural modification of the fiber and allowed hidden epitopes to be exposed. To confirm this hypothesis, we showed that a monoclonal antibody which recognizes a linear epitope of PilE bound fibers only when bacteria adhered to endothelial cells. On the other hand, this effect was not observed in PilX-deleted pili. A deletion of a region of PilX exposed on the surface of the fiber had phenotypical consequences identical to those of a PilX deletion. These data support a model in which surface-exposed motifs of PilX use forces generated by pilus retraction to promote conformational changes required for TFP-mediated signaling.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Brissac T,Mikaty G,Duménil G,Coureuil M,Nassif Xdoi
10.1128/IAI.00369-12subject
Has Abstractpub_date
2012-09-01 00:00:00pages
3297-306issue
9eissn
0019-9567issn
1098-5522pii
IAI.00369-12journal_volume
80pub_type
杂志文章abstract::Mucosal exposure to Escherichia coli elicits an inflammatory response in the urinary tract. Interleukin-6 (IL-6) is secreted into the urine, and polymorphonuclear leukocytes (PMNLs) are recruited to the site of infection. This study analyzed the ability of mucosally administered bacterial components to activate IL-6 a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.12.4357-4362.1991
更新日期:1991-12-01 00:00:00
abstract::Early-onset periodontitis (EOP) is characterized by rapidly progressive alveolar bone loss, chemotactic defects of neutrophils, and significant familial aggregation. We found immature myeloid lineage cells, defined as promyelocytes, in the peripheral blood in patients with EOP. A hematological examination of periphera...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.9.3906-3912.1997
更新日期:1997-09-01 00:00:00
abstract::Cholera toxin has been widely used to obtain insight into the cellular dynamics of the antigen-specific mucosal immune response. The present study was undertaken to clarify the influence of the organized intestinal lymphoid tissue (Peyer's patches [PP]) on the distribution of anti-cholera-toxin-containing cells (ACC) ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.9.1997-1999.1987
更新日期:1987-09-01 00:00:00
abstract::A DNA microarray containing fragments of 137 Borrelia burgdorferi B31 putative lipoprotein genes was used to examine Lyme disease spirochetes. DNA from B. burgdorferi sensu stricto B31, 297, and N40; Borrelia garinii IP90; and Borrelia afzelii P/Gau was fluorescently labeled and hybridized to the microarray, demonstra...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.6.3300-3303.2002
更新日期:2002-06-01 00:00:00
abstract::Yersinia pestis is a highly pathogenic Gram-negative organism and the causative agent of bubonic and pneumonic plague. Y. pestis is capable of causing major epidemics; thus, there is a need for vaccine targets and a greater understanding of the role of these targets in pathogenesis. Two prime Y. pestis vaccine candida...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00981-10
更新日期:2011-02-01 00:00:00
abstract::Leishmania-infected macrophages are potential antigen-presenting cells for CD4+ T lymphocytes, which recognize parasite antigens bound to major histocompatibility complex class II molecules (Ia). However, the intracellular sites where Ia and antigens may interact are far from clear, since parasites grow within the mod...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.3.764-775.1991
更新日期:1991-03-01 00:00:00
abstract::Continuous virus production is a characteristic of chicken embryo fibroblasts (CEF) infected and transformed by a nondefective Schmidt-Ruppin subgroup A Rous sarcoma virus. This virus production has been examined with particular attention to the amount of newly budded virus which remained cell-associated, and to the a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.4.834-843.1974
更新日期:1974-10-01 00:00:00
abstract::The cyst cell wall β-glucans of Pneumocystis have been shown to stimulate immune responses in lung epithelial cells, dendritic cells, and alveolar macrophages. Little is known about how the trophic life forms, which do not have a fungal cell wall, interact with these innate immune cells. Here, we report differences in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00519-16
更新日期:2016-11-01 00:00:00
abstract::Infection of CFW mice with Trichinella spiralis induced a state of relative unresponsiveness to passive cutaneous anaphylaxis (PCA) induced with hen egg albumin and its corresponding antibodies. The unresponsiveness was to PCA produced either with immunoglobulin G1 (IgG1) or IgE type of antibodies, but was more pronou...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.1.84-90.1977
更新日期:1977-01-01 00:00:00
abstract::Efficient utilization of heme as an iron (Fe) source by Bordetella avium requires bhuR, an Fe-regulated gene which encodes an outer membrane heme receptor. Upstream of bhuR is a 507-bp open reading frame, hereby designated rhuI (for regulator of heme uptake), which codes for a 19-kDa polypeptide. Whereas the 19-kDa po...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.11.6951-6961.2001
更新日期:2001-11-01 00:00:00
abstract::Pathogenicity of Rickettsia tsutsugamushi for laboratory mice is known to be influenced by at least three factors: (i) route of inoculation, (ii) antigenic strain, and (iii) natural resistance of the host. By using Karp, Gilliam, and Kato strains of R. tsutsugamushi, we examined the effect of these three pathogenicity...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.5.1476-1482.1989
更新日期:1989-05-01 00:00:00
abstract::It was shown that a strain of Serratia liquefaciens harbors a conjugative R-plasmid responsible for reistance to the following 14 antibiotics: ampicillin, carbenicillin, cephalothin, butirosin, neomycin, paramomycin, kanamycin, lividomycin, gentamicin, tobramycin, streptomycin, tetracycline, sulfonamide, and chloramph...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-06-01 00:00:00
abstract::The adipocytokine leptin links nutritional status to immune function. Leptin signaling protects from amebiasis, but the molecular mechanism is not understood. We developed an in vitro model of ameba-host cell interaction to test the hypothesis that leptin prevents ameba-induced apoptosis in host epithelial cells. We d...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.06140-11
更新日期:2012-05-01 00:00:00
abstract::Streptococcus iniae causes meningoencephalitis and death in commercial fish species and has recently been identified as an emerging human pathogen producing fulminant soft tissue infection. As identified by pulsed-field gel electrophoresis (PFGE), strains causing disease in either fish or humans belong to a single clo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.4.1994-2000.2001
更新日期:2001-04-01 00:00:00
abstract::Iron acquisition from the host is an important step in the pathogenic process. While Yersinia pestis has multiple iron transporters, the yersiniabactin (Ybt) siderophore-dependent system plays a major role in iron acquisition in vitro and in vivo. In this study, we determined that the Ybt system is required for the us...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01236-09
更新日期:2010-05-01 00:00:00
abstract::A Clostridium difficile isolate was found to produce an actin-specific ADP-ribosyltransferase (CDT) homologous to the enzymatic components of Clostridium perfringens iota toxin and Clostridium spiroforme toxin (M. R. Popoff, E. J. Rubin, D. M. Gill, and P. Boquet, Infect. Immun. 56:2299-2306, 1988). The CDT locus from...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.4.1402-1407.1997
更新日期:1997-04-01 00:00:00
abstract::Tetanus neurotoxin (TeNT) and botulinum neurotoxin (BoNT) are clostridial neurotoxins (CNTs) responsible for the paralytic diseases tetanus and botulism, respectively. CNTs are AB toxins with an N-terminal zinc-metalloprotease light chain that is linked by a disulfide bond to a C-terminal heavy chain that includes a t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01539-13
更新日期:2014-02-01 00:00:00
abstract::Antiserum to a synthetic peptide, CTP3, consisting of residues 50 to 64 of the B subunit of cholera enterotoxin (CT), a sequence which is conserved in heat-labile enterotoxins from Escherichia coli strains of human (H-LT) or porcine (P-LT) origin, cross-reacted with and neutralized all three enterotoxins. It also cros...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.52.2.562-567.1986
更新日期:1986-05-01 00:00:00
abstract::Controversy persists over the role that the capsular polysaccharide plays in the pathogenesis of Staphylococcus aureus infections. To address this issue, we compared the mouse virulence of S. aureus Reynolds and capsule-defective mutant strains cultivated under conditions of high or low capsule expression. Strain Reyn...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.11.5183-5189.1998
更新日期:1998-11-01 00:00:00
abstract::Allergic-type immune responses, particularly immunoglobulin E (IgE), correlate with protective immunity in human schistosomiasis. To better understand the mechanisms of parasite elimination we examined the immune correlates of protection in baboons (Papio cynocephalus anubis), which are natural hosts for Schistosoma m...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.2.636-642.1999
更新日期:1999-02-01 00:00:00
abstract::Humoral immunity does not play a prominent role during experimental cryptococcosis. However, previous studies have shown that immunoglobulin G (IgG) anti-Cryptococcus neoformans antibodies can mediate cell-dependent yeast killing in vitro. Therefore, the protective effect of a previously described monoclonal IgG1 anti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.3.749-752.1987
更新日期:1987-03-01 00:00:00
abstract::The present study examined the role of endogenous gamma interferon (IFN-gamma) in the formation of granulomas in mice which had been given a single intravenous injection of glycolipid-containing mycolic acid (trehalose 2,3,6'-trimycolate) purified from cell walls of Rhodococcus aurantiacus (Gordona aurantiaca) (GaGM) ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.7.2872-2878.1993
更新日期:1993-07-01 00:00:00
abstract::Targeting an antigen to Fc receptors (FcR) can enhance the immune response to the antigen in the absence of adjuvant. Furthermore, we recently demonstrated that intranasal immunization with an FcγR-targeted antigen enhances protection against a category A intracellular mucosal pathogen, Francisella tularensis. To dete...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05511-11
更新日期:2012-03-01 00:00:00
abstract::The mouse humoral immune response toward native or detergent-extracted outer membrane vesicles (NOMVs and DOMVs, respectively) from Neisseria meningitidis was determined after intranasal immunization. Both preparations elicited high frequencies of NOMV-specific antibody-forming cells (AFCs) locally in the nasal associ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.5.2528-2537.2004
更新日期:2004-05-01 00:00:00
abstract::Shiga toxin-producing Escherchia coli (STEC) comprises a group of attaching and effacing (A/E) enteric pathogens of animals and humans. Natural and experimental infection of calves with STEC may result in acute enteritis or subclinical infection, depending on serotype- and host-specific factors. To quantify intestinal...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.2.945-952.2002
更新日期:2002-02-01 00:00:00
abstract::A Mycoplasma pneumoniae cytadhesin P1 gene with novel nucleotide sequence variation has been identified. Four clinical strains of M. pneumoniae were found to carry this type of P1 gene. This new P1 gene is similar to the known group II P1 genes but possesses novel sequence variation of approximately 300 bp in the RepM...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.9.4557-4562.1999
更新日期:1999-09-01 00:00:00
abstract::We examined the effect of host age and health status on the adherence of mannose-sensitive piliated Escherichia coli K1 to human oral epithelial cells. Mannose-sensitive piliated bacteria adhered in comparable numbers to newborn, older infant, and adult cells (125 +/- 61, 198 +/- 54, and 139 +/- 69 bacteria per cell, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.1.299-301.1984
更新日期:1984-07-01 00:00:00
abstract::The abilities of six strains of Mycobacterium bovis strain BCG to replicate in vivo and to cause spleen enlargement in the CDF(1) male mouse were compared. The strains were grown in synthetic medium and harvested soon after complete pellicles were produced on liquid medium. In addition, two freeze-dried commercially a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.8.5.736-742.1973
更新日期:1973-11-01 00:00:00
abstract::CC chemokine receptor 4 (CCR4) and its two ligands, CCL17 and CCL22, are critically involved in different immune processes. In models of lipopolysaccharide-induced shock, CCR4-deficient (CCR4(-/-)) mice showed improved survival rates associated with attenuated proinflammatory cytokine release. Using CCR4(-/-) mice wit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00310-08
更新日期:2008-11-01 00:00:00
abstract::Detoxified mutants of the Escherichia coli heat-labile toxin (LT) act as mucosal adjuvants to intranasally presented coadministered antigens. Here, we compare the adjuvant activity of a panel of detoxified derivatives of LT, using both intranasal (i.n.) and oral (p.o.) routes of administration. The mutants used as adj...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.9.4400-4406.1999
更新日期:1999-09-01 00:00:00