Abstract:
:Streptococcus iniae causes meningoencephalitis and death in commercial fish species and has recently been identified as an emerging human pathogen producing fulminant soft tissue infection. As identified by pulsed-field gel electrophoresis (PFGE), strains causing disease in either fish or humans belong to a single clone, whereas isolates from nondiseased fish are genetically diverse. In this study, we used in vivo and in vitro models to examine the pathogenicity of disease-associated isolates. Strains with the clonal (disease-associated) PFGE profile were found to cause significant weight loss and bacteremia in a mouse model of subcutaneous infection. As little as 10(2) CFU of a disease-associated strain was sufficient to establish bacteremia, with higher inocula (10(7)) resulting in increased mortality. In contrast, non-disease-associated (commensal) strains failed to cause bacteremia and weight loss, even at inocula of 10(8) CFU. In addition, disease-associated strains were more resistant to phagocytic clearance in a human whole blood killing assay compared to commensal strains, which were almost entirely eradicated. Disease-associated strains were also cytotoxic to human endothelial cells as measured by lactate dehydrogenase release from host cells. However, both disease-associated and commensal strains adhered to and invaded cultured human epithelial and endothelial cells equally well. While cellular invasion may still contribute to the pathogenesis of invasive S. iniae disease, resistance to phagocytic clearance and direct cytotoxicity appear to be discriminating virulence attributes of the disease-associated clone.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Fuller JD,Bast DJ,Nizet V,Low DE,de Azavedo JCdoi
10.1128/IAI.69.4.1994-2000.2001keywords:
subject
Has Abstractpub_date
2001-04-01 00:00:00pages
1994-2000issue
4eissn
0019-9567issn
1098-5522journal_volume
69pub_type
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journal_title:Infection and immunity
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doi:10.1128/IAI.60.8.3428-3432.1992
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更新日期:1983-02-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.4.1589-1597.1992
更新日期:1992-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.4.3.256-263.1971
更新日期:1971-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/IAI.64.6.2004-2009.1996
更新日期:1996-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/IAI.58.9.3139-3142.1990
更新日期:1990-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.6.3.330-334.1972
更新日期:1972-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.3.900-906.1983
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1981-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:1997-06-01 00:00:00
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pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1994-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1978-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1978-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2003-01-01 00:00:00