Abstract:
:Mouse fibroblasts (L cells) infected with the 6BC strain of Chlamydia psittaci released potassium ion (K(+)) into the extracellular milieu in a way that depended on size of inoculum and time after infection. When the multiplicity of infection was 500 to 1,000 50% infectious units (ID(50)) per L cell, loss of intracellular K(+) was first apparent 4 to 10 h after infection and was nearly complete at 6 to 20 h. Magnesium ion and inorganic phosphate (P(i)) were also released. Similar multiplicities of ultraviolet-inactivated C. psittaci also caused release of K(+). Leakage of inorganic ions probably resulted from immediate damage to the host-cell plasma membrane during ingestion of large numbers of chlamydiae. With multiplicities of 1 to 50 ID(50) per L cell, ingestion of C. psittaci was not by itself enough to cause release of K(+) and P(i) from infected L cells. There was a delay of 36 to 72 h between infection and massive leakage of intracellular ions during which time the chlamydiae multiplied extensively. Fifty ID(50) of ultraviolet-inactivated C. psittaci per L cell did not bring about significant leakage of K(+), even after 72 h. The mechanism whereby these multiplicities of infection destroy the ability of host cells to retain intracellular molecules is not known. HeLa 229 cells also released K(+) and P(i) after infection, but these losses occurred more slowly than in comparably infected L cells, possibly because C. psittaci did not multiply as extensively in HeLa cells as it did in L cells. The significance of the inability of chlamydiae-infected cells to regulate the flow of molecules through their plasma membranes is discussed.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Chang GT,Moulder JWdoi
10.1128/IAI.19.3.827-832.1978subject
Has Abstractpub_date
1978-03-01 00:00:00pages
827-32issue
3eissn
0019-9567issn
1098-5522journal_volume
19pub_type
杂志文章abstract::Both idiopathic and infectious forms of colitis disrupt normal intestinal epithelial cell (IEC) proliferation and differentiation, although the mechanisms involved remain unclear. Recently, we demonstrated that infection by the attaching and effacing murine pathogen Citrobacter rodentium leads to a significant reducti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00655-13
更新日期:2013-12-01 00:00:00
abstract::Analysis of major outer membrane protein (MOMP) profiles of various meningococci by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (SDS-PAGE) revealed the presence of 0 to 2 low-molecular-weight, heat-modifiable MOMPs (molecular weight, 25,000 to 32,000) and 1 to 3 high-molecular-weight MOMPs (molecul...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-12-01 00:00:00
abstract::The triggering receptor expressed on myeloid cell type 1 (TREM-1) is a cell surface molecule that has been identified on both human and murine polymorphonuclear neutrophils and mature monocytes. The activation of TREM-1 in the presence of microbial components amplifies the inflammatory response and may be responsible ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.5.2823-2830.2006
更新日期:2006-05-01 00:00:00
abstract::Attaching and effacing adhesion to epithelial cells is a pathognomonic feature of infection by both enteropathogenic Escherichia coli (EPEC) and certain strains of verocytotoxin-producing E. coli (VTEC). EPEC adhesion to tissue culture epithelial cells results in activation of the phosphatidylinositol pathway, with el...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.9.3316-3326.1995
更新日期:1995-09-01 00:00:00
abstract::Advanced human periodontitis is considered to be a B-cell lesion, but the cellular infiltrate contains several cell types, the distribution of which has not been determined. This experiment was designed to characterize and identify the immunocompetent cells on histological sections and in eluates from diseased human g...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.41.1.365-374.1983
更新日期:1983-07-01 00:00:00
abstract::Mice were protected against a lethal infection with Listeria monocytogenes when treated with low doses of an anti-CD3 monoclonal antibody (MAb). Injection of anti-CD3 MAb induced rapid production of endogenous tumor necrosis factor (TNF) in the spleens and endogenous gamma interferon (IFN-gamma) in the bloodstreams an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.7.2786-2792.1993
更新日期:1993-07-01 00:00:00
abstract::We determined the diphtheria toxin phenotype of specially selected isolates of Corynebacterium ulcerans and Corynebacterium pseudotuberculosis (C. ovis). All produced proteins similar in size and immunological structure to diphtheria toxin. As with diphtheria toxin, they exhibited ADP-ribosylating activity, and their ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.3.1114-1116.1984
更新日期:1984-03-01 00:00:00
abstract::FMS-like tyrosine kinase-3 ligand (Flt3L) is a dendritic cell (DC) growth and differentiation factor with potential in antitumor therapies and antibacterial immunization strategies. However, the effect of systemic Flt3L treatment on lung-protective immunity against bacterial infection is incompletely defined. Here, we...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00854-12
更新日期:2012-12-01 00:00:00
abstract::The identification of genes with increased expression in vivo may lead to the identification of novel or unrecognized virulence traits and/or recognition of environmental signals involved in modulating gene expression. Our laboratory is studying an extraintestinal isolate of Escherichia coli as a model pathogen. We ha...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.10.5306-5314.1999
更新日期:1999-10-01 00:00:00
abstract::Dissemination of Leishmania within the host is related to parasites undergoing unchecked proliferation. We therefore studied the effects of oxidant generating systems on promastigote multiplication by (i) direct determinations of organism proliferation and (ii) the incorporation of [3H]uracil into promastigote nucleop...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.36.3.1023-1027.1982
更新日期:1982-06-01 00:00:00
abstract::Escherichia coli isolates from patients with bacteriuria of pregnancy were compared by PCR with isolates from patients with community-acquired cystitis for the presence of established virulence determinants. The strains from patients with bacteriuria of pregnancy were less likely to carry genes for P-family, S-family,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.2.794-799.2001
更新日期:2001-02-01 00:00:00
abstract::Infection with Mycobacterium avium subsp. paratuberculosis is associated with high levels of morbidity, decreased production, and early culling in dairy cattle. Clinical symptoms of Johne's disease include persistent diarrhea, inappetence, and resultant weight loss due to chronic inflammation of the small intestine. A...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.11.6479-6486.2003
更新日期:2003-11-01 00:00:00
abstract::Assembly of adherent biofilms is the key mechanism involved in Staphylococcus epidermidis virulence during device-associated infections. Aside from polysaccharide intercellular adhesin (PIA), the accumulation-associated protein Aap and the extracellular matrix binding protein Embp act as intercellular adhesins, mediat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01142-10
更新日期:2011-06-01 00:00:00
abstract::Hamster (Mesocricetus auratus) neutrophil granules contain at least four microbicidal peptides belonging to the defensin family. These compounds were purified from granule acid extracts by reverse-phase chromatography and termed HaNP-1 to -4 (hamster neutrophil peptide). HaNP-1 and HaNP-3 revealed the most bactericida...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.11.4444-4449.1996
更新日期:1996-11-01 00:00:00
abstract::To determine the protective efficacy of human hyperimmune globulin to Haemophilus influenzae type b disease in an infant rat model, we compared hyperimmune globulin containing 600 mug of anti-polyribophosphate (PRP) antibody per ml to conventional immune globulin containing 66 mug of anti-PRP antibody per ml. The hype...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.2.709-714.1983
更新日期:1983-02-01 00:00:00
abstract::Mucosal immune responses to fungal infection range from T helper type 2 (Th2) cell-directed allergic inflammation to Th1-predominant neutrophilic inflammation, but the mechanisms directing these divergent mucosal immune outcomes and the role of T cells in host defense against mucosal fungal infections are not known. H...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05209-11
更新日期:2011-11-01 00:00:00
abstract::The affinity of uropathogenic Escherichia coli to kidneys and bladders of experimentally infected mice was shown to be determined in part by the adhesive properties of the infecting bacteria. Mice were infected with various pairwise combinations of two homogeneic sets of bacteria: (i) mutants derived from a human pyel...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.1.265-272.1983
更新日期:1983-04-01 00:00:00
abstract::Gamma interferon (IFN-gamma) is produced in response to circulating lipopolysaccharide (LPS) and contributes to the lethality of endotoxic shock. To address the cellular source of IFN-gamma production in vivo, T cells and B cells were magnetically purified from C57BL/6 mouse spleens 5 h following endotoxin injection. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.10.4244-4249.1994
更新日期:1994-10-01 00:00:00
abstract::Cell-free hemoglobin (Hb) is being developed as an erythrocyte substitute. We have previously demonstrated that cell-free Hb is an endotoxin-binding protein which disaggregates endotoxin and subsequently increases the biological activity of endotoxin in several in vitro assays. Because much of the morbidity and mortal...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.4.1258-1266.1997
更新日期:1997-04-01 00:00:00
abstract::Mucosa-associated invariant T (MAIT) cells are an innate-like T cell subset in mammals that recognize microbial vitamin B metabolites presented by the evolutionarily conserved major histocompatibility complex class I (MHC I)-related molecule, MR1. Emerging data suggest that MAIT cells may be an attractive target for v...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00524-20
更新日期:2020-12-15 00:00:00
abstract::Dendritic cells (DC) suffer a maturation defect following interaction with erythrocytes infected with malaria parasites and become unable to induce protective malaria liver-stage immunity. Here we show that, by contrast, maturation-arrested DC in vitro are capable of the successful induction of antigen-specific gamma ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.9.5331-5339.2004
更新日期:2004-09-01 00:00:00
abstract::Staphylococcus epidermidis is a leading cause of infections associated with indwelling medical devices, including prosthetic joint infection. While biofilm formation is assumed to be the main mechanism underlying the chronic infections S. epidermidis causes, we hypothesized that S. epidermidis also evades immune killi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00237-18
更新日期:2018-09-21 00:00:00
abstract::Several trivalent cations, including lanthanum (La3+), inhibited the secretion (enterosorption) induced by the enterotoxins of Vibrio cholerae and Escherichia coli in the rabbit ileum in vivo. High concentrations (greater than 10 mM) of La3+ were required to inhibit cholera enterotoxin (CE)-induced enterosorption, pro...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.5.1038-1044.1975
更新日期:1975-05-01 00:00:00
abstract::Filarial infection is initiated by mosquito-derived third-stage larvae (L3) deposited on the skin that transit through the epidermis, which contains Langerhans cells (LC) and keratinocytes (KC), among other cells. This earliest interaction between L3 and the LC likely conditions the priming of the immune system to the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01301-12
更新日期:2013-05-01 00:00:00
abstract::A2G mice are genetically resistant to lethal infection with neurotropic and pneumotropic influenza viruses. A possible immunological explanation for this resistance was sought by assessing the effect of cyclophosphamide and X irradiation immunosuppression on the infection of A2G mice with lethal doses of neurovirulent...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.3.576-587.1975
更新日期:1975-03-01 00:00:00
abstract::We have recently found that strain B31 infection-immune rabbits are completely protected against homologous challenge with large numbers (>10(6)) of host-adapted Borrelia burgdorferi (HAB) (E. S. Shang, C. I. Champion, X. Wu, J. T. Skare, D. B. Blanco, J. N. Miller, and M. A. Lovett, Infect. Immun. 68:4189-4199, 2000)...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.1.593-598.2001
更新日期:2001-01-01 00:00:00
abstract::Candida albicans is a major opportunistic pathogen in immunocompromised patients. Production of proinflammatory cytokines by host cells in response to C. albicans plays a critical role in the activation of immune cells and final clearance of the organism. Invasion of host cells and tissues is considered one of the vir...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.8.4588-4595.2005
更新日期:2005-08-01 00:00:00
abstract::The antiphagocytic effect of the Staphylococcus aureus capsule is known to be related to its ability to interfere with opsonization by normal human serum. In this study, evidence is presented with isolated cell surface components which indicates that the capsule hinders opsonization by masking cell wall peptidoglycan....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.23.2.502-508.1979
更新日期:1979-02-01 00:00:00
abstract::Aspergillus species are saprophytic molds causing life-threatening invasive fungal infections in the immunocompromised host. Innate immune recognition, in particular, the mechanisms of opsonization and complement activation, has been reported to be an integral part of the defense against fungi. We have shown that the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00032-13
更新日期:2013-05-01 00:00:00
abstract::A virus-specific cell surface antigen(s) was detected on mouse adenovirus-infected cells by the immunofluorescent antibody technique. It was demonstrated in infected cells regardless of the mouse strain or animal species used. It became detectable on the cell surface about 6 h postinfection and was demonstrated in 90%...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.18.1.41-45.1977
更新日期:1977-10-01 00:00:00