Survival of Staphylococcus epidermidis in Fibroblasts and Osteoblasts.

Abstract:

:Staphylococcus epidermidis is a leading cause of infections associated with indwelling medical devices, including prosthetic joint infection. While biofilm formation is assumed to be the main mechanism underlying the chronic infections S. epidermidis causes, we hypothesized that S. epidermidis also evades immune killing, contributing to its pathogenesis. Here, we show that prosthetic joint-associated S. epidermidis isolates can persist intracellularly within human fibroblasts and inside human and mouse osteoblasts. We also show that the intracellularly persisting bacteria reside primarily within acidic phagolysosomes and that over the course of infection, small-colony variants are selected for. Moreover, upon eukaryotic cell death, these bacteria, which can outlive their host, can escape into the extracellular environment, providing them an opportunity to form biofilms on implant surfaces at delayed time points in implant-associated infection. In summary, the acidic phagolysosomes of fibroblasts and osteoblasts serve as reservoirs for chronic or delayed S. epidermidis infection.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Perez K,Patel R

doi

10.1128/IAI.00237-18

subject

Has Abstract

pub_date

2018-09-21 00:00:00

issue

10

eissn

0019-9567

issn

1098-5522

pii

IAI.00237-18

journal_volume

86

pub_type

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