Screening of sera for antibodies to foot-and-mouth disease viral antigens by radial immunodiffusion.

abstract：:Vibrio cholerae responds to environmental changes by altering the protein composition of its outer membrane. In rich medium, V. cholerae expresses almost exclusively the outer membrane porin OmpU, whereas in minimal medium, OmpT is the dominant porin. The supplementation of a minimal medium with a mixture of asparagin...

journal_title：Infection and immunity

authors： Mey AR,Craig SA,Payne SM

更新日期：2012-02-01 00:00:00

abstract：:The Haemophilus ducreyi 35000HP genome encodes a homolog of the CpxRA two-component cell envelope stress response system originally characterized in Escherichia coli. CpxR, the cytoplasmic response regulator, was shown previously to be involved in repression of the expression of the lspB-lspA2 operon (M. Labandeira-Re...

journal_title：Infection and immunity

authors： Labandeira-Rey M,Brautigam CA,Hansen EJ

更新日期：2010-11-01 00:00:00

abstract：:Potential vaccine components for protection against atrophic rhinitis in pigs were developed. This was achieved by deletion mutagenesis of the gene encoding the Pasteurella multocida toxin. Four purified toxin derivatives lacking different and widely separated regions in the amino acid sequence were characterized by a...

journal_title：Infection and immunity

authors： Petersen SK,Foged NT,Bording A,Nielsen JP,Riemann HK,Frandsen PL

更新日期：1991-04-01 00:00:00

abstract：:Urogenital Chlamydia serovars replicating in reproductive epithelium pose a unique challenge to host immunity and vaccine development. Previous studies have shown that CD4 T cells are necessary and sufficient to clear primary Chlamydia muridarum genital tract infections in the mouse model, making a protective CD4 T ce...

journal_title：Infection and immunity

authors： Johnson RM,Yu H,Kerr MS,Slaven JE,Karunakaran KP,Brunham RC

更新日期：2012-06-01 00:00:00

abstract：:Serologic parameters of kala-azar were evaluated by Western blot analysis. Sera from kala-azar patients with confirmed diagnoses were screened for immunoglobulin G (IgG) and IgG subclass-specific reactivity against Leishmania donovani membrane antigen (LAg). Heterogeneous LAg-specific IgG reactivity with numerous prot...

journal_title：Infection and immunity

authors： Ravindran R,Anam K,Bairagi BC,Saha B,Pramanik N,Guha SK,Goswami RP,Banerjee D,Ali N

更新日期：2004-02-01 00:00:00

abstract：:Culture fluids after growth of Mycobacterium bovis BCG on Sauton medium contain actively secreted proteins and proteins released by bacterial lysis. BCG culture fluids and sonicates of Mycobacterium tuberculosis and Mycobacterium paratuberculosis were tested after separation by gel filtration and sodium dodecyl sulfat...

journal_title：Infection and immunity

authors： Harboe M,Wiker HG,Ulvund G,Lund-Pedersen B,Andersen AB,Hewinson RG,Nagai S

更新日期：1998-01-01 00:00:00

abstract：:Insertional mutagenesis studies of mrpB, a putative pilin-encoding open reading frame of the mrp gene cluster, which encodes mannose-resistant Proteus-like (MR/P) fimbriae of Proteus mirabilis, indicate that MrpB functions as the terminator for fimbrial assembly. ...

journal_title：Infection and immunity

authors： Li X,Mobley HL

更新日期：1998-04-01 00:00:00

abstract：:The induction of interleukin-8 (IL-8) in vitro has been suggested to correlate with the reactogenicity of Vibrio cholerae vaccine candidates. V. cholerae vaccine candidate 638, a hemagglutinin protease/hap-defective strain, was recently reported to be well tolerated in human volunteers, suggesting a role for Hap in re...

journal_title：Infection and immunity

authors： Zhou X,Gao DQ,Michalski J,Benitez JA,Kaper JB

更新日期：2004-01-01 00:00:00

abstract：:Gamma interferon (IFN-gamma) is produced in response to circulating lipopolysaccharide (LPS) and contributes to the lethality of endotoxic shock. To address the cellular source of IFN-gamma production in vivo, T cells and B cells were magnetically purified from C57BL/6 mouse spleens 5 h following endotoxin injection. ...

journal_title：Infection and immunity

authors： Heinzel FP,Rerko RM,Ling P,Hakimi J,Schoenhaut DS

更新日期：1994-10-01 00:00:00

abstract：:Peptidoglycans of organisms belonging to the strictly anaerobic family Bacteroidaceae were investigated for the presence of lanthionine. Different procedures for the quantitation of lanthionine were compared. Performic acid and peroxide oxidation procedures on 35S-labeled peptidoglycan from Fusobacterium nucleatum Fev...

journal_title：Infection and immunity

authors： Vasstrand EN,Jensen HB,Miron T,Hofstad T

更新日期：1982-04-01 00:00:00

abstract：:The glucosyltransferases (GTFs) of viridans streptococci, common pathogens of infective endocarditis, are extracellular proteins that convert sucrose into exopolysaccharides and glucans. GTFs B, C, and D of Streptococcus mutans are modulins that induce, in vitro and in vivo, the production of cytokines, in particular ...

journal_title：Infection and immunity

authors： Shun CT,Lu SY,Yeh CY,Chiang CP,Chia JS,Chen JY

更新日期：2005-06-01 00:00:00

abstract：:Little data are available regarding L-form phagocyte interactions. Due to difficulty with more conventional methods, a serum-free technique of surface phagocytosis employing cellulose ester micropore filters, fluorochrome staining, and reflecting fluorescence microscopy was developed. Intracellular localization of L-f...

journal_title：Infection and immunity

authors： Harwick HJ,Barajas L,Montgomerie JZ,Kalmanson GM,Guze LB

更新日期：1972-06-01 00:00:00

abstract：:Vitamin E protects nonimmunized and immunized mice against fatal Diplococcus pneumoniae type I (DpI) infection. A dietary supplementation of 180 mg of dl-alpha-tocopheryl acetate per kg of diet increased survival of nonimmunized mice from 20 to 80% when challenged with 20 organisms, and of mice immunized with 0.5 ng o...

journal_title：Infection and immunity

authors： Heinzerling RH,Tengerdy RP,Wick LL,Lueker DC

更新日期：1974-12-01 00:00:00

abstract：:Neonatal animals are generally very susceptible to infection with bacterial pathogens. However, we recently reported that neonatal mice are highly resistant to orogastric infection with Yersinia enterocolitica. Here, we show that proinflammatory responses greatly exceeding those in adults arise very rapidly in the mes...

journal_title：Infection and immunity

authors： Siefker DT,Echeverry A,Brambilla R,Fukata M,Schesser K,Adkins B

更新日期：2014-02-01 00:00:00

abstract：:Because of the association of the group A streptococcal pyrogenic exotoxins (SPEs) with erythrogenic toxin used in the classical Dick test, the involvement of the SPEs in production of erythematous skin reactions was assessed. Unless they had been presensitized, young adult rabbits failed to show skin reactions after ...

journal_title：Infection and immunity

authors： Schlievert PM,Bettin KM,Watson DW

更新日期：1979-11-01 00:00:00

abstract：:Two proteins, HifD and HifE, have been identified as structural components of Haemophilus influenzae pili. Both are localized at the pilus tip, and HifE appears to mediate pilus adherence to host cells. In this study we examined the immunologic and structural diversity of these pilus subunits among type b H. influenza...

journal_title：Infection and immunity

authors： McCrea KW,Sauver JL,Marrs CF,Clemans D,Gilsdorf JR

更新日期：1998-10-01 00:00:00

abstract：:Actinlike material was obtained from disrupted Pseudomonas aeruginosa cells by a modification of the method of Hancock and Nikaido (J. Bacteriol. 136:381-390) for isolating outer membrane vesicles. Pelleted membranes were dissolved in Laemmli sample buffer and electrophoresed in parallel lanes with purified rabbit ske...

journal_title：Infection and immunity

authors： Berk RS,Moon M,Higbee R,Cramer C,Roher A,Weis M,Hazlett L

更新日期：1985-11-01 00:00:00

abstract：:Pertussis toxin is secreted from Bordetella pertussis with the assistance of the Ptl transport system, a member of the type IV family of macromolecular transporters. The S1 subunit and the B oligomer combine to form the holotoxin prior to export from the bacterial cell, although the site of assembly is not known. To b...

journal_title：Infection and immunity

authors： Farizo KM,Fiddner S,Cheung AM,Burns DL

更新日期：2002-03-01 00:00:00

abstract：:A strain of a nutritionally deficient streptococcus was shown to undergo morphological aberrations according to pyridoxal concentrations in the growth medium. Filamentous rod-shaped cells, observed by electron microscopy, predominated in the presence of decreasing concentrations. Multiple invaginations in the outer ce...

journal_title：Infection and immunity

authors： Clark RB,Gordon RE,Bottone EJ,Reitano M

更新日期：1983-10-01 00:00:00

abstract：:We demonstrate that transcription of the Staphylococcus aureus collagen adhesin gene (cna) is temporally regulated, with expression being highest in exponentially growing cultures and falling to almost undetectable levels as cultures enter the post-exponential-growth phase. The temporal regulation of cna transcription...

journal_title：Infection and immunity

authors： Gillaspy AF,Patti JM,Smeltzer MS

更新日期：1997-04-01 00:00:00

abstract：:The primary effector for Shigella invasion of epithelial cells is IpaC, which is secreted via a type III secretion system. We recently reported that the IpaC N terminus is required for type III secretion and possibly other functions. In this study, mutagenesis was used to identify an N-terminal secretion signal and to...

journal_title：Infection and immunity

authors： Harrington AT,Hearn PD,Picking WL,Barker JR,Wessel A,Picking WD

更新日期：2003-03-01 00:00:00

abstract：:A 10-amino-acid repeating sequence of the hemagglutinating portion of Clostridium difficile toxin A has been synthesized and used to produce antisera in rabbits. Antipeptide antibody inhibited toxin A-mediated hemagglutination and neutralized cytotoxic activity. Immunoblot analysis with the antipeptide antibody reveal...

journal_title：Infection and immunity

authors： Wren BW,Russell RR,Tabaqchali S

更新日期：1991-09-01 00:00:00

abstract：:Although often considered a strict human pathogen, Streptococcus pneumoniae has been reported to infect and cause pneumonia in horses, although the pathology appears restricted compared to that of human infections. Here we report on the molecular characterization of a group of S. pneumoniae isolates obtained from hors...

journal_title：Infection and immunity

authors： Whatmore AM,King SJ,Doherty NC,Sturgeon D,Chanter N,Dowson CG

更新日期：1999-06-01 00:00:00

abstract：:Highly purified protein antigens are usually poor immunogens; in practice, adjuvants are needed to obtain satisfactory immune responses. Plasmodium yoelii 19-kDa merozoite surface protein 1 (MSP1(19)) is a weak antigen, but mice vaccinated with this antigen in strong adjuvants can survive an otherwise lethal parasite ...

journal_title：Infection and immunity

authors： Ogun SA,Dumon-Seignovert L,Marchand JB,Holder AA,Hill F

更新日期：2008-08-01 00:00:00

abstract：:Streptococcus pneumoniae remains a major causative agent of serious human diseases. The worldwide increase of antibiotic resistant strains revealed the importance of horizontal gene transfer in this pathogen, a scenario that results in the modulation of the species-specific gene pool. We investigated genomic variation...

journal_title：Infection and immunity

authors： Hakenbeck R,Balmelle N,Weber B,Gardès C,Keck W,de Saizieu A

更新日期：2001-04-01 00:00:00

abstract：:Immune responses in the guinea pig model are understudied because of a lack of commercial reagents. We have developed a custom-made guinea pig oligonucleotide microarray (81 spots) and have examined the gene expression profile of splenocytes restimulated in vitro from Mycobacterium bovis BCG-vaccinated and naive anima...

journal_title：Infection and immunity

authors： Tree JA,Elmore MJ,Javed S,Williams A,Marsh PD

更新日期：2006-02-01 00:00:00

abstract：:Shiga toxin-producing Escherchia coli (STEC) comprises a group of attaching and effacing (A/E) enteric pathogens of animals and humans. Natural and experimental infection of calves with STEC may result in acute enteritis or subclinical infection, depending on serotype- and host-specific factors. To quantify intestinal...

journal_title：Infection and immunity

authors： Stevens MP,Marchès O,Campbell J,Huter V,Frankel G,Phillips AD,Oswald E,Wallis TS

更新日期：2002-02-01 00:00:00

abstract：:Various invasive pathogens attach to host tissues via the extracellular matrix component laminin, the major glycoprotein found within basement membranes. Previous investigations identified the laminin-binding adhesin Tp0751 within the spirochete bacterium Treponema pallidum. In the current study, Tp0751 was shown to a...

journal_title：Infection and immunity

authors： Cameron CE,Brouwer NL,Tisch LM,Kuroiwa JM

更新日期：2005-11-01 00:00:00

abstract：:Neospora caninum is a recently identified coccidian parasite which shares many features with, but is clearly distinct from, Toxoplasma gondii. N. caninum tachyzoites infect a wide range of mammalian cells both in vivo and in vitro. The mechanisms by which infection is achieved are largely unknown. Recent evidence has ...

journal_title：Infection and immunity

authors： Hemphill A

更新日期：1996-10-01 00:00:00

abstract：:We show in this study that toxin production in Clostridium difficile is altered in cells which can no longer form flagellar filaments. The impact of inactivation of fliC, CD0240, fliF, fliG, fliM, and flhB-fliR flagellar genes upon toxin levels in culture supernatants was assessed using cell-based cytotoxicity assay, ...

journal_title：Infection and immunity

authors： Aubry A,Hussack G,Chen W,KuoLee R,Twine SM,Fulton KM,Foote S,Carrillo CD,Tanha J,Logan SM

更新日期：2012-10-01 00:00:00