Abstract:
:Gamma interferon (IFN-gamma) is produced in response to circulating lipopolysaccharide (LPS) and contributes to the lethality of endotoxic shock. To address the cellular source of IFN-gamma production in vivo, T cells and B cells were magnetically purified from C57BL/6 mouse spleens 5 h following endotoxin injection. IFN-gamma RNA was abundant in splenic CD4+ and CD8+ T cells and in a T- and B-cell-depleted population of splenocytes containing 34% NK1.1+ natural killer (NK) cells. Because interleukin 12 (IL-12) is a known inducer of IFN-gamma synthesis by cultured T cells and NK cells, we examined whether IL-12 might be involved in IFN-gamma release during endotoxemia. mRNA encoding the p40 subunit of IL-12 increased markedly in the spleens of C57BL/6 mice at 2 h after LPS injection, whereas p35 IL-12 mRNA was constitutively expressed at all times. Bioactive IL-12 (p70 heterodimer) was detected in mouse serum at 2 to 4 h after LPS injection. Similar results were obtained using a p40 subunit-specific enzyme-linked immunosorbent assay. Endotoxin-insensitive C3H/HeJ mice generated threefold less IL-12 p70 and IFN-gamma at these times than endotoxin-sensitive C3H/HeOuJ mice. Pretreatment of mice with polyclonal anti-mouse IL-12 antibody reduced IFN-gamma levels present at 6 h post-LPS nearly sixfold in three separate experiments. These studies support a role for IL-12 as a proximal stimulator of IFN-gamma release during endotoxemia.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Heinzel FP,Rerko RM,Ling P,Hakimi J,Schoenhaut DSdoi
10.1128/IAI.62.10.4244-4249.1994subject
Has Abstractpub_date
1994-10-01 00:00:00pages
4244-9issue
10eissn
0019-9567issn
1098-5522journal_volume
62pub_type
杂志文章abstract::Humans infected with the dimorphic fungus Blastomyces dermatitidis develop strong T-lymphocyte responses to WI-1, an immunodominant antigen that has been shown to elicit protective immunity in mice. In the present study, the T-cell epitopes of WI-1 and human leukocyte antigen (HLA) restricting elements that display th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.2.502-510.2000
更新日期:2000-02-01 00:00:00
abstract::Antibodies against the Plasmodium falciparum P0 ribosomal phosphoprotein (PfP0) have been detected exclusively but extensively in malaria-immune persons. Polyclonal rabbit and mice sera were raised against two recombinant polypeptides of P. falciparum P0 protein, PfP0N and PfP0C, covering amino acids 17 to 61 and the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.7.4312-4318.2000
更新日期:2000-07-01 00:00:00
abstract::T-cell-mediated immunity is an important determinant in protection against primary infection with Coccidioides immitis, a dimorphic fungal pathogen that causes the disease coccidioidomycosis. To determine if interleukin-12 (IL-12) gene therapy could potentiate host response against C. immitis, we constructed a single-...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.6.2996-3001.1999
更新日期:1999-06-01 00:00:00
abstract::Campylobacter jejuni is the leading cause of infectious gastroenteritis in industrialized nations. Its ability to enter and survive within nonphagocytic cells is thought to be very important for pathogenesis. However, little is known about the C. jejuni determinants that mediate these processes. Through an extensive t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00109-10
更新日期:2010-08-01 00:00:00
abstract::Patients with chronic bronchitis are colonized by and may develop acute bronchopulmonary infection due to nontypable Haemophilus influenzae (NTHI) despite the presence of bactericidal and opsonizing antibody to the infecting organism. To test the hypothesis that secretory immunoglobulin A (IgA) interferes with host de...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.1.36-40.1984
更新日期:1984-07-01 00:00:00
abstract::In 2001, a bioterrorism attack involving Bacillus anthracis spore-laced letters resulted in 22 cases of inhalation anthrax, with five fatalities. This incident identified gaps in our health care system and precipitated a renewed interest in identifying both therapeutics and rapid diagnostic assays. To address those ga...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00520-08
更新日期:2008-12-01 00:00:00
abstract::We examined the reactivities of Clostridium butyricum neurotoxin to nine monoclonal antibodies against Clostridium botulinum type E neurotoxin which recognize the light chain or the amino-terminal half (H-1 fragment) or the carboxyl-terminal half (H-2 fragment) of the heavy chain of botulinum neurotoxin. Butyricum neu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.1.457-459.1991
更新日期:1991-01-01 00:00:00
abstract::Pseudomonas aeruginosa elastase rapidly inactivates alpha 1-proteinase inhibitor by splitting its Pro-357-Met-358 peptide bond. The present study was aimed at testing whether this reaction takes place in the presence of leukocyte elastase. To this end was added alpha 1-proteinase inhibitor to a mixture of the two elas...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.12.3793-3797.1989
更新日期:1989-12-01 00:00:00
abstract::Sonicated preparations of capsulated Haemophilus influenzae type b, two of its spontaneous mutants, one containing patches of capsules (class I variant) and the other noncapsulated (class II variant), and a noncapsulated strain of H. influenzae type d were analyzed by crossed-immunoelectrophoresis using unadsorbed ant...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.6.1733-1742.1976
更新日期:1976-06-01 00:00:00
abstract::The Lyme disease spirochete, Borrelia burgdorferi, exists in a zoonotic cycle involving an arthropod tick and mammalian host. Dissemination of the organism within and between these hosts depends upon the spirochete's ability to traverse through complex tissues. Additionally, the spirochete outruns the host immune cell...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01228-12
更新日期:2013-06-01 00:00:00
abstract::Lipid compositions of 40 patient isolates of Mycobacterium tuberculosis derived from Madras, Burma, Rangoon, and East Africa were studied, and two major populations of tubercle bacilli were distinguished. Nearly all of the strains previously designated as attenuated for the guinea pig (D. A. Mitchison) are characteriz...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.1.150-158.1974
更新日期:1974-01-01 00:00:00
abstract::Purified group A streptococcal peptidoglycan-polysaccharide (PG-PS) fragments were either de-O-acylated, or acetylated and then de-O-acylated to yield N-acetylated PG-PS. Native PG-PS was poorly degraded, N-acetylated PG-PS was extensively degraded, and de-O-acylated PG-PS was only slightly degraded by hen egg white l...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.1.16-23.1987
更新日期:1987-01-01 00:00:00
abstract::Arg-gingipain (Rgp) and Lys-gingipain (Kgp) are Porphyromonas gingivalis cysteine proteinases implicated as major virulence factors in pathologies of periodontitis. We purified a 660-kDa cell-associated gingipain complex existing as a homodimer of two catalytically active monomers which comprises their catalytic and a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.2.883-893.2005
更新日期:2005-02-01 00:00:00
abstract::During infection, the Legionnaires' disease bacterium, Legionella pneumophila, survives and multiplies within a specialized phagosome that is near neutral pH and does not fuse with host lysosomes. In order to understand the molecular basis of this organism's ability to control its intracellular fate, we have isolated ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1998-09-01 00:00:00
abstract::We have recently reported Bacillus anthracis attenuated live vaccine strains efficiently expressing recombinant protective antigen (rPA) and have shown a direct correlation between the level of rPA secreted by these cells and efficacy (S. Cohen, I. Mendelson, Z. Altboum, D. Kobiler, E. Elhanany, T. Bino, M. Leitner, I...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.2.801-813.2003
更新日期:2003-02-01 00:00:00
abstract::An antigenically distinct serotype, type IV, has recently been added to the recognized serotypes of group B streptococci (GBS). We isolated and purified the capsular polysaccharide antigen from a prototype type IV GBS strain. The type IV capsular polysaccharide formed a precipitin line with rabbit antiserum to type IV...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.4.1089-1094.1989
更新日期:1989-04-01 00:00:00
abstract::Previous studies have suggested that the Pseudomonas aeruginosa PAK pilus adhesin moiety resides in an epithelial cell-binding domain located in the C-terminal region of the PAK pilin structural protein. Synthetic peptides Ac17red (a synthetic peptide with a sequence identical to that of PAK pilin residues 128 to 144,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.12.3720-3726.1989
更新日期:1989-12-01 00:00:00
abstract::The capacity of the intracellular pathogen Listeria monocytogenes to activate the alternative pathway of human complement was examined. Incubation of L. monocytogenes with human serum in optimal conditions (20% Mg2+EGTA [ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid]-chelated serum) consumed (3...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.12.5134-5139.1993
更新日期:1993-12-01 00:00:00
abstract::The antibacterial properties of lysozyme were investigated with oral microorganisms representing the seven serotypes (a through g) of Streptococcus mutans, Veillonella alcalescens, and the virulent (V) and avirulent (AV) strains of Actinomyces viscosus T14. Growth of bacteria in defined medium was monitored spectropho...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-08-01 00:00:00
abstract::Virulent Treponema pallidum organisms, extracted from infected rabbit testes, were subjected to velocity sedimentation in discontinuous gradients of Hypaque, a high density, low viscosity material. After centrifugation of extracts at 20 C for 45 min at 100,000 x g, treponemes separated into two distinct bands based up...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.5.1062-1067.1974
更新日期:1974-11-01 00:00:00
abstract::Escherichia coli F-18 is a human fecal isolate that makes type 1 fimbriae, encoded by the fim gene cluster, and is an excellent colonizer of the streptomycin-treated mouse intestine. E. coli F-18 fimA::tet, lacking type 1 fimbriae, was constructed by bacteriophage P1 transduction of the fim region of the E. coli K-12 ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.9.3497-3503.1996
更新日期:1996-09-01 00:00:00
abstract::Toxigenic Vibrio cholerae strains are lysogens of CTXPhi, a filamentous phage which encodes cholera toxin. The receptor for CTXPhi for invading V. cholerae cells is the toxin-coregulated pilus (TCP), the genes for which reside in a larger genetic element, the TCP pathogenicity island. We analyzed 146 CTX-negative stra...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.12.5819-5825.1998
更新日期:1998-12-01 00:00:00
abstract::Brucella organisms are intracellular stealth pathogens of animals and humans. The bacteria overcome the assault of innate immunity at early stages of an infection. Removal of polymorphonuclear neutrophils (PMNs) at the onset of adaptive immunity against Brucella abortus favored bacterial elimination in mice. This was ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00118-19
更新日期:2019-04-23 00:00:00
abstract::The bundle-forming pilus (BFP) of enteropathogenic Escherichia coli (EPEC) is an important virulence factor. We examined the role of divergent alleles of bfpA encoding bundlin, the BFP pilin protein, in pilus biogenesis, pilus interactions, and immune responses. We found that the BFP biogenesis machine from an EPEC st...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00009-07
更新日期:2007-10-01 00:00:00
abstract::Screening of a Mycobacterium tuberculosis genomic DNA library in the lambda gt11 expression vector was carried out by using, as probes, sera from tuberculous patients and murine monoclonal antibody H61.3 recognizing a mycobacterial 35-kilodalton protein present only on the M. tuberculosis complex. The recombinant beta...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.1.245-251.1990
更新日期:1990-01-01 00:00:00
abstract::Modulation of myelopoiesis by three synthetic muramyl peptides was investigated in vivo. Two adjuvant-active compounds (N-acetylmuramyl dipeptide [MDP] and MDP-butyl-ester) elicited significant responses in DBA/2 mice characterized by a rise in the level of monocyte-macrophage colony-stimulating activity in serum, a p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.3.1081-1085.1983
更新日期:1983-12-01 00:00:00
abstract::The mucosal humoral immune response elicited following Shigella flexneri infection in patients living in Antananarivo districts (Madagascar Island) was evaluated by measuring the gut-derived, circulating immunoglobulin A (IgA) antibody-secreting cells (ASC) specific for the major bacterial antigen lipopolysaccharide (...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.69.9.5230-5234.2001
更新日期:2001-09-01 00:00:00
abstract::Brucellosis is a bacterial disease of animals and humans. Brucella abortus barely activates the innate immune system at the onset of infection, and this bacterium is resistant to the microbicidal action of complement. Since complement stands as the first line of defense during bacterial invasions, we explored the role...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00567-18
更新日期:2018-09-21 00:00:00
abstract::Both dextransucrase and mutansynthetase activities have been purified from the culture fluids of Streptococcus mutans GS-5 (serotype c). Although homogeneous dextransucrase preparations normally synthesize little insoluble glucan, essentially all of the glucan synthesized by this enzyme in the presence of 1.5 M (NH4)2...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.2.763-770.1983
更新日期:1983-11-01 00:00:00
abstract::Membrane and soluble fractions of Mycoplasma pneumoniae, M. pulmonis, and M. laidlawii B were prepared by hypotonic lysis of whole cells. The membranes of M. pneumoniae and M. laidlawii B contained, as percentage of dry weight: 34 to 37% protein, 59 to 61% lipid, 3 to 4% carbohydrate as hexose, and 0.2% ribonucleic ac...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.2.3.326-339.1970
更新日期:1970-09-01 00:00:00