Abstract:
:Membrane and soluble fractions of Mycoplasma pneumoniae, M. pulmonis, and M. laidlawii B were prepared by hypotonic lysis of whole cells. The membranes of M. pneumoniae and M. laidlawii B contained, as percentage of dry weight: 34 to 37% protein, 59 to 61% lipid, 3 to 4% carbohydrate as hexose, and 0.2% ribonucleic acid as ribose. NADH(2) and NADPH(2) oxidase activities were localized in the soluble fractions of M. pneumoniae and in the membrane fraction of M. laidlawii B. NADH(2) oxidase activity was localized in the soluble fraction of M. pulmonis. The lipids of M. pneumoniae were labeled when the organism was grown in the presence of either radioactive palmitic acid, oleic acid, cholesterol, or glycerol. The lipids were not labeled when grown in the presence of radioactive acetate. Palmitic acid radio-activity was found in neutral lipid, glycolipid, and phosphatide fractions. Immunodiffusion analyses of whole cells and membrane fractions demonstrated three reactive antigens. Two immunodiffusion antigens were localized in the membrane fraction. One of these apparently contains lipid. A third antigen, also considered lipoidal, was found in whole cells. Membrane and soluble fractions of M. pneumoniae were immunogenic. The immunogens eliciting metabolic-inhibiting antibodies were localized in the membrane. The membrane preparation also induced the formation of antibodies which fixed complement with an antigen extracted with lipid solvent. The soluble fraction contained a distinct immunogen which induces antibodies reactive in complement fixation with an antigen prepared by phenol extraction.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Pollack JD,Somerson NL,Senterfit LBdoi
10.1128/IAI.2.3.326-339.1970keywords:
subject
Has Abstractpub_date
1970-09-01 00:00:00pages
326-39issue
3eissn
0019-9567issn
1098-5522journal_volume
2pub_type
杂志文章abstract::Peripheral leukocytes from 16 Thai children with dengue hemorrhagic fever were examined to determine the leukocyte composition on the day of presentation and on convalescent days 15 and 30. Mononuclear cells were isolated each time, and the concentrations of T, B, Fc receptor-bearing, and "null" cells were determined....
journal_title:Infection and immunity
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abstract::Borrelia burgdorferi produces potent cell-activating molecules capable of stimulating polyclonal proliferation and immunoglobulin production by murine B lymphocytes and cytokine production by a variety of cell types. These stimulatory molecules function in infected mice, resulting in elevated levels of circulating imm...
journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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doi:10.1128/IAI.43.3.846-849.1984
更新日期:1984-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-06-01 00:00:00
abstract::Arthritis is a frequent and major complication of infection with Borrelia burgdorferi sensu stricto. The antigens responsible for the induction of arthritis are unknown. Here we provide direct evidence that a major surface protein, outer surface protein A (OspA), can induce arthritis. Hamsters were vaccinated with 30,...
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pub_type: 杂志文章
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更新日期:2000-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01589-13
更新日期:2014-05-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1983-02-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1998-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.10.4033-4037.1993
更新日期:1993-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.8.3.442-445.1973
更新日期:1973-09-01 00:00:00
abstract::In this analysis we have examined in detail the effects of low concentrations of zinc on the growth and dimorphism of Candida albicans. Evidence is presented that micromolar concentrations of zinc added to growth cultures grown at 25 degrees C (i) cause a twofold increase in the final concentration of spheres at satio...
journal_title:Infection and immunity
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doi:10.1128/IAI.26.1.348-354.1979
更新日期:1979-10-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.56.11.2918-2924.1988
更新日期:1988-11-01 00:00:00
abstract::Helicobacter pylori has been directly linked with active chronic gastritis, peptic ulceration, and gastric adenocarcinoma in humans. Although a substantial portion of the human population is colonized with H. pylori, the patterns of transmission of the organism remain in doubt, and reservoir hosts have not been identi...
journal_title:Infection and immunity
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doi:10.1128/IAI.62.6.2367-2374.1994
更新日期:1994-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.41.2.702-708.1983
更新日期:1983-08-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.58.5.1384-1390.1990
更新日期:1990-05-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.58.3.835-837.1990
更新日期:1990-03-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.40.1.46-55.1983
更新日期:1983-04-01 00:00:00
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journal_title:Infection and immunity
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更新日期:2009-10-01 00:00:00
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journal_title:Infection and immunity
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abstract::Haemophilus ducreyi expresses 2 OmpA homologs, designated MOMP and OmpA2, whose genes are arranged in tandem on the chromosome. Northern blot analysis indicated that momp and ompA2 are transcribed independently. Sequences of the momp open reading frame (ORF) lacking the transcriptional start site were amplified by PCR...
journal_title:Infection and immunity
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abstract::Toll-like receptors and Nod-like receptors (NLR) play an important role in sensing invading microorganisms for pathogen clearance and eliciting adaptive immunity for protection against rechallenge. Nod1 and Nod2, members of the NLR family, are capable of detecting bacterial peptidoglycan motifs in the host cytosol for...
journal_title:Infection and immunity
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