Abstract:
:The absolute requirement for elemental iron and the porphyrin nucleus for growth of Haemophilus influenzae led us to investigate the role of iron and hemin in regulation of expression of the H. influenzae transferrin receptor. H. influenzae type b strain H1689 was grown in brain heart infusion broth supplemented with beta-NAD and either 10 or 0.1 microgram of hemin ml-1. Transferrin-binding ability was determined with a dot blot assay using human transferrin-horseradish peroxidase conjugate. Cells grown in media with 0.1 microgram of hemin ml-1 bound transferrin, but organisms grown in media with 10 micrograms ml-1 did not. In hemin-restricted media, transferrin binding occurred despite addition of up to 10 mM ferric nitrate, ferric citrate, or ferric PPi, whereas addition of 10 micrograms of hemoglobin ml-1 repressed expression. The breadth of species distribution of this mode of regulation was determined with strains previously characterized by multilocus enzyme electrophoresis. When grown in hemin-restricted media, 24 of 28 type b strains and 52 of 57 serologically nontypeable strains exhibited transferrin binding, although none did so in hemin- and iron-sufficient media. Strain H1689 and serologically nontypeable strain HI1423 grown in heat-inactivated pooled normal human serum, human cerebrospinal fluid, or human breast milk exhibited transferrin binding. Growth in these fluids with 10 micrograms of added hemin ml-1 abolished transferrin binding, whereas addition of 10 mM ferric nitrate did not. These data suggest that the transferrin receptor of H. influenzae is regulated by levels of hemin but not elemental iron alone and that this property is widely distributed among several major cloned families in the species.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Morton DJ,Musser JM,Stull TLdoi
10.1128/IAI.61.10.4033-4037.1993subject
Has Abstractpub_date
1993-10-01 00:00:00pages
4033-7issue
10eissn
0019-9567issn
1098-5522journal_volume
61pub_type
杂志文章abstract::Highly purified protein antigens are usually poor immunogens; in practice, adjuvants are needed to obtain satisfactory immune responses. Plasmodium yoelii 19-kDa merozoite surface protein 1 (MSP1(19)) is a weak antigen, but mice vaccinated with this antigen in strong adjuvants can survive an otherwise lethal parasite ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01369-07
更新日期:2008-08-01 00:00:00
abstract::Monoclonal antibodies to Trichomonas vaginalis were prepared by immunizing mice with a cloned isolate of T. vaginalis. Eight antibodies reacted with the same four isolates or strains but did not react with the other T. vaginalis strains or isolates tested. All eight antibodies reacted uniformly with both the body and ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.1.270-275.1984
更新日期:1984-01-01 00:00:00
abstract::The two published sequences of the pertussis toxin operon differ in 3 bp in the S1 subunit gene. In this report, we provide evidence that Bordetella pertussis strains are able to produce active pertussis toxin only when they contain one of the two possible nucleotide sequences. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.3.1177-1179.1991
更新日期:1991-03-01 00:00:00
abstract::Trichomonas vaginalis infected with a double-stranded RNA virus undergoes phenotypic variation on the basis of surface versus cytoplasmic expression of the immunogenic protein P270. Examination of batch cultures by flow cytofluorometry with monoclonal antibody (MAb) to P270 yields both fluorescent and nonfluorescent t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.8.4298-4302.1999
更新日期:1999-08-01 00:00:00
abstract::Infectious pancreatic necrosis virus was stable for 10 days at 4 C in stream and well water, after which the virus had a half-life of 7.5 days. At 15 C, the virus was stable for 5 days, and then had a half-life between 5 and 6 days. Viral antigen in infected cells developed much more slowly at 4 C than at 20 C. Infect...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.6.1409-1412.1975
更新日期:1975-06-01 00:00:00
abstract::Escherichia coli is a versatile pathogen causing millions of infections in humans every year. This bacterium can form multicellular aggregates when it expresses a self-associating protein, antigen 43 (Ag43), on its surface. We have discovered that Ag43-expressing E. coli cells are efficiently taken up by human defense...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01117-06
更新日期:2007-01-01 00:00:00
abstract::All of the Salmonella enteritidis strains isolated from diseased animals (61 strains) and from beef (2 strains) in Japan and in West Germany (1 strain), except for 2 strains isolated from ducks, harbored either a 36-megadalton (Md) plasmid alone or in combination with several other plasmids of different sizes. It is l...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.47.3.831-833.1985
更新日期:1985-03-01 00:00:00
abstract::Nontypeable Haemophilus influenzae (NTHi) is a commensal microorganism of the human nasopharynx, and yet is also an opportunistic pathogen of the upper and lower respiratory tracts. Host microenvironments influence gene expression patterns, likely critical for NTHi persistence. The host sequesters iron as a mechanism ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01227-12
更新日期:2013-04-01 00:00:00
abstract::Ehrlichia chaffeensis, a tick-transmitted obligate intracellular rickettsial agent, causes human monocytic ehrlichiosis. In recent reports, we described substantial advances in developing random and targeted gene disruption methods to investigate the functions of E. chaffeensis genes. We reported earlier that the Hima...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00316-20
更新日期:2020-09-18 00:00:00
abstract::Urease from ureaplasmas was purified by immunoaffinity chromatography, and the N-terminal amino acid sequence was determined for two of the three subunits. These sequences were used to design primers for a polymerase chain reaction (PCR) that amplified most of the gene coding for one of the subunits. By using a novel ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.7.2463-2469.1991
更新日期:1991-07-01 00:00:00
abstract::Porphyromonas gingivalis is a gram-negative anaerobic bacterium that is considered the key etiologic agent of chronic periodontitis. Arg- and Lys-gingipain cysteine proteinases produced by P. gingivalis are key virulence factors and are believed to be essential for significant tissue component degradation, leading to ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.8.4689-4698.2004
更新日期:2004-08-01 00:00:00
abstract::An enzyme-linked immunosorbent assay was developed to detect rabbit immunoglobulin G antibodies against purified lipopolysaccharide of the rough Re mutant of Salmonella minnesota. The time necessary for each step was investigated, and this resulted in a test that could be completed in 1 working day. Serial dilutions o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.3.721-728.1978
更新日期:1978-09-01 00:00:00
abstract::Borrelia burgdorferi, the causative agent of Lyme disease, loses its ability to infect and cause disease in mammalian hosts after repeated in vitro passage. To identify proteins preferentially expressed by the low-passage strain and thus representing potential virulence factors, the polypeptide profiles of virulent, l...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.11.4662-4672.1992
更新日期:1992-11-01 00:00:00
abstract::Peptic ulcer disease (PUD) occurs after a long-term Helicobacter pylori infection. However, the disease can develop earlier, and rare cases have been observed in children, suggesting that these H. pylori strains may be more virulent. We used suppressive subtractive hybridization for comparative genomics between H. pyl...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00123-06
更新日期:2006-07-01 00:00:00
abstract::The energy-dependent adsorption of radioiodinated rickettsiae to sheep erythrocytes was demonstrated. The iodination procedure, however, decreased the hemolytic activity of the rickettsiae. No desorption of rickettsiae from isolated rickettsia-erythrocyte complexes (prevented from lysing by NaF) could be measured. On ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.17.3.607-612.1977
更新日期:1977-09-01 00:00:00
abstract::Bacterial superantigens (SAgs) have been implicated in inflammatory disease, and SAg-treated mice have increased jejunal T cells. Here we show that T84 cells (a human epithelial cell line) display increased MCP-1 and RANTES mRNA expression and protein production in response to conditioned medium from Staphylococcus au...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.11.6198-6202.1999
更新日期:1999-11-01 00:00:00
abstract::The effect of indomethacin on the net intestinal accumulation of fluid induced by Escherichia coli heat-stable (ST) enterotoxin in the infant mouse model was examined. Indomethacin, when administered with ST enterotoxin, caused a striking decrease in net intestinal fluid accumulation. This inhibition of ST activity wa...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.22.1.143-147.1978
更新日期:1978-10-01 00:00:00
abstract::Several strains of group B Streptococcus agalactiae were found to be lethal for young adult rats. When bacteria were heat killed and then injected intraperitoneally into rats, rapid death (14 to 18 h) of the rats occurred, characterized by labored breathing, hemolyzed serum, hemoglobinuria, and subungual hemorrhages. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.48.2.560-564.1985
更新日期:1985-05-01 00:00:00
abstract::The purpose of our study was to characterize the properties of the interaction of pertussis toxin with human polymorphonuclear leukocytes for the modulation of leukotriene generation and metabolism. The cells were stimulated with either the Ca ionophore A23187, opsonized zymosan, or the bacterial peptide formyl-methio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.10.3165-3171.1989
更新日期:1989-10-01 00:00:00
abstract::Lack of efficacious vaccines against Mycobacterium tuberculosis (MTB) infection is a limiting factor in the prevention and control of tuberculosis (TB), the leading cause of death from an infectious agent. Improvement or replacement of the BCG vaccine with one that reliably protects all age-groups is urgent. Concerns ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00585-20
更新日期:2020-12-14 00:00:00
abstract::Neonatal animals are generally very susceptible to infection with bacterial pathogens. However, we recently reported that neonatal mice are highly resistant to orogastric infection with Yersinia enterocolitica. Here, we show that proinflammatory responses greatly exceeding those in adults arise very rapidly in the mes...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01489-13
更新日期:2014-02-01 00:00:00
abstract::Staphylococcus aureus Newman with an insertion mutation in clfB, the gene encoding clumping factor B, only marginally decreased infection rate (P>0.05) in rats with experimental endocarditis. In contrast, clfB complementation on a multicopy plasmid significantly increased infectivity (P<0.05) over the deleted mutants....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.9.5443-5446.2000
更新日期:2000-09-01 00:00:00
abstract::Escherichia coli C25 maintained population levels of 10(9) to 10(10) per g of cecum and translocated to 100% of the middle mesenteric lymph nodes in gnotobiotic mice monoassociated with E. coli C25. Intragastric inoculation of these mice with the cecal contents from specific-pathogen-free mice reduced the population l...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-09-01 00:00:00
abstract::Bordetella bronchiseptica is closely related to Bordetella pertussis, which produces respiratory disease primarily in mammals other than humans. However, its importance as a human pathogen is being increasingly recognized. Although a large amount of research on Bordetella has been generated regarding protein virulence...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.4.1791-1798.2002
更新日期:2002-04-01 00:00:00
abstract::Salmonella infection can cause gastroenteritis in healthy individuals or a serious, systemic infection in immunocompromised patients and has a global impact. CD4 Th1 cells represent the main lymphocyte population that participates in bacterial clearance during both primary and secondary infections in mice of the H-2b ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00588-19
更新日期:2019-10-18 00:00:00
abstract::To determine the ultrastructural distribution and immunological accessibility of proteins II (P.IIs) on the surfaces of whole gonococci, anti-P.II gold probes were developed and used in electron microscopic studies of viable P.II-expressing variants of Neisseria gonorrhoeae FA1090. Anti-P.II probes clearly marked the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.4.1003-1006.1988
更新日期:1988-04-01 00:00:00
abstract::The amino acid sequence of heat-stable enterotoxin produced by enterotoxigenic Escherichia coli 18D has been revised. Amino acids originally assigned to positions 11 and 18, i.e., Tyr and Asn, respectively, were found to occupy positions 18 and 11, respectively. Thus all heat-stable enterotoxins composed of 18 amino a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.47.3.834-836.1985
更新日期:1985-03-01 00:00:00
abstract::Four pneumococcal genes (phtA, phtB, phtD, and phtE) encoding a novel family of homologous proteins (32 to 87% identity) were identified from the Streptococcus pneumoniae genomic sequence. These open reading frames were selected as potential vaccine candidates based upon their possession of hydrophobic leader sequence...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.2.949-958.2001
更新日期:2001-02-01 00:00:00
abstract::The antiphagocytic effect of the Staphylococcus aureus capsule is known to be related to its ability to interfere with opsonization by normal human serum. In this study, evidence is presented with isolated cell surface components which indicates that the capsule hinders opsonization by masking cell wall peptidoglycan....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.23.2.502-508.1979
更新日期:1979-02-01 00:00:00
abstract::The adsorption of (3)H-labeled Streptococcus mutans 6715 cells to disks of hydroxyapatite (HA) was studied. The number of streptococci that adsorbed was logarithmically related to the concentration of cells available up to at least 2 x 10(8) per ml; equilibrium occurred within 45 min. Assay reliability was verified by...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.18.2.514-523.1977
更新日期:1977-11-01 00:00:00