Abstract:
:The adsorption of (3)H-labeled Streptococcus mutans 6715 cells to disks of hydroxyapatite (HA) was studied. The number of streptococci that adsorbed was logarithmically related to the concentration of cells available up to at least 2 x 10(8) per ml; equilibrium occurred within 45 min. Assay reliability was verified by direct scanning electron microscopic counts. Untreated HA disks exposed to buffered saline (PBS)-suspended streptococci at a concentration of 1.1 x 10(8) per ml absorbed 3.2 x 10(6) cells per cm(2); approximately 3% of the surface area was, therefore, occupied by adsorbed organisms. The presence of adsorbed salivary components on HA reduced the number of attaching S. mutans cells by half. When S. mutans cells were suspended in saliva to mimic conditions existing in the mouth, the number of streptococci adsorbing to saliva-treated HA was reduced more than 30-fold compared to untreated HA. Approximately one-half of the streptococci adsorbed to untreated or to saliva-treated HA disks could be desorbed over a 4-h period with 0.067 M phosphate buffer. S. mutans cells exposed to sucrose to permit extracellular polysaccharide synthesis before or during adsorption attached in fewer numbers to both saliva-treated and untreated HA than PBS-treated organisms. When S. mutans cells adsorbed on untreated HA were exposed to sucrose, fewer organisms could be desorbed; thus, in situ polysaccharide synthesis promoted their more firm attachment to untreated HA. However, when saliva-suspended streptococci were adsorbed to saliva-treated HA surfaces, exposure to sucrose before or subsequent to adsorption did not promote more firm attachment. Evidently, the powerful adherence-inhibiting and desorptive effects of salivary components overshadowed any promoting effects attributable to glucan synthesis from sucrose. Similarly, no differences were noted in the desorption of S. mutans cells from human teeth after exposure to sucrose, glucose, or PBS relative to a strain of Streptococcus mitis (S. mitior). Thus, no evidence was obtained to support the hypothesis that glucan synthesis from sucrose was essential for, or promoted, the attachment of S. mutans cells to HA surfaces exposed to saliva or to the smooth surfaces of human teeth.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Clark WB,Gibbons RJdoi
10.1128/IAI.18.2.514-523.1977subject
Has Abstractpub_date
1977-11-01 00:00:00pages
514-23issue
2eissn
0019-9567issn
1098-5522journal_volume
18pub_type
杂志文章abstract::Strains of Bacteroides fragilis that produce a ca. 20-kDa heat-labile protein toxin (termed B. fragilis toxin [BFT]) have been associated with diarrheal disease of animals and humans. BFT alters the morphology of intestinal epithelial cells both in vitro and in vivo and stimulates secretion in ligated intestinal segme...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.3.1007-1013.1997
更新日期:1997-03-01 00:00:00
abstract::Legionella pneumophila organisms are able to infect and multiply within the ciliated protozoan Tetrahymena pyriformis. This ability may be associated with virulence, because an attenuated strain of L. pneumophila fails to multiply within this protozoan, whereas a virulent strain increases 10,000-fold in number when co...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.53.3.553-559.1986
更新日期:1986-09-01 00:00:00
abstract::Serotype b-specific polysaccharide antigen (SPA) was extracted from whole cells of Actinobacillus actinomycetemcomitans Y4 by autoclaving and purified by chromatography on DEAE-Sephadex A-25 and Sephacryl S-300. SPA induced the release of monocyte and leukocyte chemotactic factors by human monocytes. Polymyxin B had a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.7.2563-2570.1996
更新日期:1996-07-01 00:00:00
abstract::Legionella pneumophila was susceptible to the antimicrobial action of oxygen metabolites generated by both the myeloperoxidase-H(2)O(2)-halide and the xanthine oxidase systems. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.1.487-489.1983
更新日期:1983-01-01 00:00:00
abstract::Seven antigens of Borrelia hermsii, B. parkeri, and B. turicatae with isoelectric points in the range of 4.4 to 5.0 and molecular masses of 40 to 43 kilodaltons played a role in the relapse phenomenon of relapsing fever. Based upon location of the antigens in the outer envelope, the molecular weight, and Western blot ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.3.899-902.1986
更新日期:1986-12-01 00:00:00
abstract::Mice (BALB/cJ, C3H/HeN, and C3H/HeJ) primed with actinomycin D became highly susceptible to lethal intoxication with staphylococcal enterotoxin B (SEB). The mice underwent toxicosis and toxic shock and died. Actinomycin D-primed C3H/HeN and C3H/HeJ mice showed equal sensitivity to SEB, suggesting that bacterial lipopo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.10.4626-4631.1994
更新日期:1994-10-01 00:00:00
abstract::Cells of the marine fish pathogen Vibrio anguillarum 775 harboring a plasmid associated with virulence can grow unaffected in the presence of iron-binding compounds such as transferrin. In contrast, the growth of isogenic plasmidless derivatives is inhibited by the presence of iron chelators. Radioactive from (55Fe3+)...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.1.223-227.1981
更新日期:1981-01-01 00:00:00
abstract::Nonspecific interactions related to physicochemical properties of bacterial cell surfaces, such as hydrophobicity and electrostatic charge, are known to have important roles in bacterium-host cell encounters. Streptococcus pneumoniae (pneumococcus) expresses multiple, surface-exposed, choline-binding proteins (CBPs) w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.1.412-415.2002
更新日期:2002-01-01 00:00:00
abstract::Cholera toxin has been widely used to obtain insight into the cellular dynamics of the antigen-specific mucosal immune response. The present study was undertaken to clarify the influence of the organized intestinal lymphoid tissue (Peyer's patches [PP]) on the distribution of anti-cholera-toxin-containing cells (ACC) ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.9.1997-1999.1987
更新日期:1987-09-01 00:00:00
abstract::Exoantigens of Plasmodium vivax-parasitized erythrocytes stimulated macrophages to secrete tumor necrosis factor, and antisera raised against the exoantigens inhibited this secretion. The antisera also inhibited the activity of Plasmodium falciparum and Plasmodium yoelii exoantigens, and conversely, antisera against t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.3.1241-1243.1992
更新日期:1992-03-01 00:00:00
abstract::In this study we have investigated the antibody and CD4 T-cell responses to the well-characterized malaria vaccine candidate MSP-1 during the course of a primary Plasmodium chabaudi chabaudi (AS) infection. Specific antibody responses can be detected within the first week of infection, and CD4 T cells can be detected ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.4.2245-2251.2001
更新日期:2001-04-01 00:00:00
abstract::Tumor necrosis factor alpha (TNF) is thought to play a major role in the pathogenesis of septic shock. Anti-TNF antibody was preadministered in low-dose endotoxin lethality models in which BALB/c mice were challenged with small amounts of lipopolysaccharide following their sensitization with either carrageenan (CAR) o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.8.2609-2614.1991
更新日期:1991-08-01 00:00:00
abstract::Cell wall lytic enzyme (Kyowa lytic no. 2 enzyme) liberated arthritogenic hydrosoluble peptidoglycans from both arthritogenic and non-arthritogenic bacterial cell walls. From these cell walls, mutanolysin (peptidoglycan-degrading enzyme) also liberated hydrosoluble peptidoglycans which, however, lacked arthritogenicit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.3.861-866.1977
更新日期:1977-06-01 00:00:00
abstract::Murine hybridoma cell lines were developed which synthesized monoclonal antibodies against Actinobacillus actinomycetemcomitans-associated antigens. Monoclonal antibodies specific for an antigen(s) common to all A. actinomycetemcomitans isolates tested but not detected on other gram-negative oral plaque microorganisms...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.5.1394-1398.1988
更新日期:1988-05-01 00:00:00
abstract::After interaction with tracheal epithelial cells, Bordetella pertussis induces the secretion of interleukin-6. This secretion is dependent on the expression of adenylate cyclase-hemolysin by the bacterium but not on the expression of other characterized bacterial toxins or adhesins. This finding confirms the important...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.9.5530-5533.2004
更新日期:2004-09-01 00:00:00
abstract::Brucella abortus 2308 derivatives with mini-Tn5 insertions in purE, purL, and purD display significant attenuation in the BALB/c mouse model, while isogenic mutants with mini-Tn5 insertions in pheA, trpB, and dagA display little or no attenuation in cultured murine macrophages or mice. These experimental findings conf...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.8.4911-4917.2004
更新日期:2004-08-01 00:00:00
abstract::Type II heat-labile enterotoxin (LT-II) from Escherichia coli 41 was purified and compared with prototype LT-II encoded by genes from E. coli SA53. Both toxins were oligomeric proteins consisting of polypeptides A (Mr, 28,000) and B (Mr, 11,800). The A polypeptides were cleaved by trypsin into fragments A1 (Mr, 21,000...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.2.529-536.1986
更新日期:1986-11-01 00:00:00
abstract::Paracoccidioidomycosis patients show hyperactive humoral immune responses. Consequently, we investigated whether cytokines in supernatants from Paracoccidioides brasiliensis-stimulated gamma/delta T cells support B-cell activation. We detected proliferation of B cells and increased immunoglobulin M (IgM) and IgG produ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.4.1608-1610.1995
更新日期:1995-04-01 00:00:00
abstract::Resequencing estA3, an allele of the methanol-soluble heat-stable enterotoxin of Escherichia coli showed that the proline triplet 19 is in fact an alanine codon; thus, estA alleles 3 and 4 were shown to be identical. Resequencing has also shown that the carboxy terminus of another allele, estA2, is not the previously ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.2.645-648.1989
更新日期:1989-02-01 00:00:00
abstract::During bacterial infection of the bovine mammary gland, large numbers of leukocytes migrate into the udder, resulting in the establishment of a host response against the pathogen. Currently, the specific leukocyte populations mediating this immune response are not well defined. In the studies described here, we analyz...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::Mice with a null deletion mutation in the gamma interferon (IFN-gamma) receptor gene were used to study the role of IFN-gamma responsiveness during experimental pulmonary cryptococcosis. Cryptococcus neoformans was inoculated intratracheally into mice lacking the IFN-gamma receptor gene (IFN-gammaR-/-) and into contro...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.3.1788-1796.2005
更新日期:2005-03-01 00:00:00
abstract::A 2.9-kilobase pair locus in Neisseria meningitidis was identified as containing transcriptionally linked open reading frames encoding TolC- and HlyD-like proteins. Although the meningococcal TolC protein was required for extracellular production of the repeats-in-toxin (RTX) FrpC toxin, it could not functionally repl...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01995-06
更新日期:2007-12-01 00:00:00
abstract::Pseudomonas aeruginosa utilizes a number of distinct pathways to secrete proteins that play various roles during infection. These include the type II secretion system, which is responsible for the secretion of the majority of exoproducts into the surrounding environment, including toxins and degradative enzymes. In co...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.3.1695-1705.2005
更新日期:2005-03-01 00:00:00
abstract::Disruption of neutrophils causes the release of a cytoplasmic protein which can inhibit the growth of Candida albicans without killing the organisms. The present study was undertaken with a combined system in which candidacidal and candidastatic activities of human neutrophils could be compared. In this system it was ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.8.2696-2698.1990
更新日期:1990-08-01 00:00:00
abstract::Three-month-old male Crl:CD1(ICR)BR and C3H/HeJ mice were fed chow containing Candida albicans for 14 days, while similar control mice were fed regular food. Stool cultures were done for all mice before and after administration of the special diet. Stool cultures were repeated 48 h, 1 week, and 1 and 3 months after st...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.6.1514-1517.1990
更新日期:1990-06-01 00:00:00
abstract::Ring-necked pheasant virus, an avian leukosis virus, when injected into 10-day old chick embryos, caused angiosarcomas in the lungs of infected chickens within a short time. Angiosarcomas appeared as localized foci of proliferating cells in the lungs as early as 2 weeks posthatch, and by 6 weeks, the lungs of the infe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.1.310-319.1983
更新日期:1983-04-01 00:00:00
abstract::Enteropathogenic strains of Yersinia enterocolitica harbor a virulence plasmid (70 kilobases) which specifies, at 37 degrees C, a calcium requirement for growth, autoagglutinability, resistance to the bactericidal activity of human serum, and the expression of some outer membrane proteins (OMPs). To map the genes enco...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.48.3.782-786.1985
更新日期:1985-06-01 00:00:00
abstract::The urease gene cluster from the virulent Actinobacillus pleuropneumoniae serotype 1 strain CM5 was cloned and sequenced. The urease activity was associated with a 6.3-kbp region which contains eight long open reading frames (ORFs). The structural genes, ureABC, are separated from the accessory genes, ureEFGD, by a 61...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.11.4389-4394.1997
更新日期:1997-11-01 00:00:00
abstract::Studies of the effect of sex hormones on the susceptibility of mice to the disseminated gonococcal infection demonstrated significantly enhanced susceptibility of mice injected with estrogen (17 beta-estradiol). In mice treated with estradiol, bacteremia progressively developed within 12 h postinoculation and mice die...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.49.1.238-243.1985
更新日期:1985-07-01 00:00:00
abstract::Although excystation is crucial to the initiation of infection by Giardia lamblia, little is known about the regulation of this important process. We have been able to reliably induce excystation in vitro by mimicking cyst passage through the stomach and upper small intestine by the exposure of in vitro-derived cysts ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.6.2151-2157.1996
更新日期:1996-06-01 00:00:00