Abstract:
:The amino acid sequence of heat-stable enterotoxin produced by enterotoxigenic Escherichia coli 18D has been revised. Amino acids originally assigned to positions 11 and 18, i.e., Tyr and Asn, respectively, were found to occupy positions 18 and 11, respectively. Thus all heat-stable enterotoxins composed of 18 amino acids sequenced to date from human, porcine, and bovine isolates of E. coli have identical primary structures, i.e., Asn-Thr-Phe-Tyr-Cys-Cys-Glu-Leu-Cys-Cys-Asn-Pro-Ala-Cys-Ala-Gly-Cys-Tyr. Furthermore, all 18- and 19-amino-acid heat-stable enterotoxins from E. coli share an almost identical core sequence, i.e., 14 of the 15 carboxy-terminal amino acid residues are identical.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Thompson MR,Giannella RAdoi
10.1128/IAI.47.3.834-836.1985subject
Has Abstractpub_date
1985-03-01 00:00:00pages
834-6issue
3eissn
0019-9567issn
1098-5522journal_volume
47pub_type
杂志文章abstract::The diarrheagenic pathogen enteropathogenic Escherichia coli (EPEC) is responsible for significant infant mortality and morbidity, particularly in developing countries. EPEC pathogenesis relies on a type III secretion system-mediated transfer of virulence effectors into host cells. EPEC modulates host cell survival an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01690-06
更新日期:2007-05-01 00:00:00
abstract::Splenic lymphocytes from cytomegalovirus-infected mice lost their in vitro proliferative responses to cytomegalovirus antigen within 3 h after in vivo treatment with antilymphocyte globulin and prednisolone. The response was inhibited when the agents were administered separately or together, and inhibition persisted t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-12-01 00:00:00
abstract::The 70-kilobase pYV plasmid of Yersinia enterocolitica encodes a set of proteins called Yops that are produced during infection. To use Y. enterocolitica as a live carrier to present the cholera toxin B (CT-B) subunit to the immune system, we constructed an operon fusion between ctxB and the yop51 gene. This operon fu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.8.2420-2428.1990
更新日期:1990-08-01 00:00:00
abstract::Previous reports from our laboratory have shown that 7 days after infection of C3HeB/FeJ mice with an attenuated strain of Salmonella typhimurium, there is profound suppression of responses to B- and T-cell mitogens and suppression of the capacity of spleen cells to mount a primary, in vitro plaque-forming-cell (PFC) ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.9.3786-3792.1996
更新日期:1996-09-01 00:00:00
abstract::Outbred Swiss mice were inoculated intraperitoneally or intravenously with one 90 to 100% lethal dose of Escherichia coli O:18, Proteus mirabilis, or Klebsiella pneumoniae. After carefully timed intervals, aminoglycoside antibiotics were begun at dosages nnd intervals predetermined to constitute optimal therapy. With ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.25.2.538-557.1979
更新日期:1979-08-01 00:00:00
abstract::Saccharomyces boulardii is a nonpathogenic yeast that protects against antibiotic-associated diarrhea and recurrent Clostridium difficile colitis. The administration of C. difficile toxoid A by gavage to S. boulardii-fed BALB/c mice caused a 1.8-fold increase in total small intestinal immunoglobulin A levels (P = 0.00...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.4.2762-2765.2001
更新日期:2001-04-01 00:00:00
abstract::Three strains of anaerobic, dextranase-producing, gram-positive, rod-shaped bacteria were isolated from human dental plaque associated with root carious lesions. The isolates produced a molar ratio of acetate to lactate from glucose fermentation ranging from 1.1 to 1.9. Each strain also produced fructose-6-phosphate p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.2.716-720.1983
更新日期:1983-11-01 00:00:00
abstract::The rfb region from Vibrio cholerae O139 strain MO45 was cloned from cosmid gene banks established in Escherichia coli HB101, using an immunoblot assay for screening of the correct clones. Immunoblot analysis of lipopolysaccharide (LPS) preparations revealed the presence of two types of positive clones: (i) those expr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.9.3565-3570.1996
更新日期:1996-09-01 00:00:00
abstract::The gene encoding the 35-kDa immunogenic Treponema pallidium subsp. pallidum (T. pallidum) membrane protein C, TmpC, was cloned, sequenced, and expressed in Escherichia coli. The deduced amino acid sequence carries on N-terminal signal sequence with a four-amino-acid motif, which is characteristic for bacterial lipopr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.10.3536-3546.1991
更新日期:1991-10-01 00:00:00
abstract::Metastatic infection after hematogenous dissemination of Candida species is presumably dependent on the fungus traversing the vascular endothelium. An in vitro model of the earliest events of metastatic Candida infection was developed with whole vascular strips. Freshly obtained porcine blood vessels were secured in a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.1.374-384.1983
更新日期:1983-10-01 00:00:00
abstract::A total of 133 Listeria monocytogenes isolates were characterized by ribotyping and allelic analysis of the virulence genes hly, actA, and inlA to uncover linkages between independent phylogenetic and specific virulence markers. PCR-restriction fragment length polymorphisms revealed 8 hly, 11 inl4, and 2 actA alleles....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.7.2707-2716.1997
更新日期:1997-07-01 00:00:00
abstract::Adhesion of Trichomonas vaginalis is believed to be dependent on four adhesion proteins, which are thought to bind to vaginal epithelial cells in a specific manner with a ligand-receptor type of interaction. However, the specific receptors on the host cell have not yet been identified. In this work, the ability of the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.7.4358-4360.2000
更新日期:2000-07-01 00:00:00
abstract::Transforming growth factor beta (TGF-beta) has been shown to be a central immunomodulator used by leishmaniae to escape effective mechanisms of protection in human and murine infections with these parasites. However, all the information is derived from studies of established infection, while little is known about TGF-...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.9.5908-5914.2005
更新日期:2005-09-01 00:00:00
abstract::Escherichia coli strains isolated from newborn infants were injected intraperitoneally into infant rats. Strains possessing the K1 capsular polysaccharide antigen were significantly more virulent than strains lacking this antigen. When 5-day-old animals were injected with 1.2 X 10(1) colony forming units of a K1 E. co...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.22.2.480-485.1978
更新日期:1978-11-01 00:00:00
abstract::During the infection of mice with Listeria monocytogenes, there is a profound depletion of T (Thy-1+ Ig-) lymphocytes between days 1 and 4, followed by an increase in T cells to three times normal levels by day 9. The recovery of T cell numbers required cell proliferation, being sensitive to vinblastin and cyclophosph...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.2.694-698.1982
更新日期:1982-11-01 00:00:00
abstract::Ehrlichia chaffeensis is an obligately intracellular gram-negative bacterium and is the etiologic agent of human monocytic ehrlichiosis (HME). Although E. chaffeensis induces the generation of several cytokines and chemokines by leukocytes, E. chaffeensis lacks lipopolysaccharide and peptidoglycan. Bioinfomatic analys...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00056-08
更新日期:2008-08-01 00:00:00
abstract::Major research efforts are directed towards the development of a better antimycobacterial vaccine. But progress in the field of tuberculosis vaccine development has been hampered by the lack of human in vitro models to assess vaccine immunogenicity and efficacy. New candidate vaccines will have to be evaluated against...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.11.6401-6407.2004
更新日期:2004-11-01 00:00:00
abstract::We produced anti-idiotype antibodies to antibody to lipid A from Eikenella corrodens. The ALA-1 monoclonal antibody (immunoglobulin M [IgM] isotype), which had already been produced in our laboratory (T. Kato, I. Takazoe, and K. Okuda, Infect. Immun. 57:656-659, 1989), had reacted strongly with lipid A from E. corrode...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.2.416-420.1990
更新日期:1990-02-01 00:00:00
abstract::Legionella pneumophila multiplied rapidly in guinea pig and rat alveolar macrophages but failed to grow when phagocytic activity was inhibited by pretreatment with 0.5 or 1.0 microgram of cytochalasin D per ml. Attachment was not inhibited by cytochalasin D. No extracellular multiplication occurred when L. pneumophila...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.51.1.31-36.1986
更新日期:1986-01-01 00:00:00
abstract::Borrelia burgdorferi synthesizes a variety of differentially regulated outer surface lipoproteins in the tick vector and in vertebrate hosts. Among these is OspD, a protein that is highly induced in vitro by conditions that mimic the tick environment. Using genetically engineered strains in which ospD is deleted, we d...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00714-07
更新日期:2008-05-01 00:00:00
abstract::When phagocytosis of Staphylococcus aureus by human polymorphonuclear leukocytes (PMN) takes place on the surface of cultured human endothelial cells, the endothelial monolayers are damaged by lysosomal enzymes that are released by the PMN. Because PMN can phagocytose opsonized as well as unopsonized staphylococci on ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.6.1455-1460.1987
更新日期:1987-06-01 00:00:00
abstract::Penetration of the intestinal mucosa at areas of Peyer's patches is an important first step for Salmonella typhimurium to produce lethal systemic disease in mice. However, mutations in genes that are important for intestinal invasion result in only moderately decreased virulence of S. typhimurium for mice. Here we rep...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.6.2254-2259.1997
更新日期:1997-06-01 00:00:00
abstract::We have previously demonstrated that fibronectin mediates streptococcal adhesion to host cells and that streptococci interact primarily with the N-terminal domain of fibronectin. FBP54 is a 54-kDa protein from group A streptococci that binds fibronectin. In this report, we show that the N-terminal domain of fibronecti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.7.2415-2419.1996
更新日期:1996-07-01 00:00:00
abstract::Burkholderia cenocepacia is an opportunistic pathogen that primarily infects cystic fibrosis (CF) patients. Previously, we reported that ShvR, a LysR regulator, influences colony morphology, virulence, and biofilm formation and regulates the expression of an adjacent 24-kb genomic region encoding 24 genes. In this stu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00170-11
更新日期:2011-08-01 00:00:00
abstract::Previous work in our laboratory has shown that corticosterone increases the susceptibility of macrophages from Bcgs mice to the growth of Mycobacterium avium. The innate antimycobacterial activity of macrophages from Bcgr mice was not affected by corticosterone. In contrast to the differential effect of corticosterone...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.8.2983-2988.1995
更新日期:1995-08-01 00:00:00
abstract::Peptic ulcer disease (PUD) occurs after a long-term Helicobacter pylori infection. However, the disease can develop earlier, and rare cases have been observed in children, suggesting that these H. pylori strains may be more virulent. We used suppressive subtractive hybridization for comparative genomics between H. pyl...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00123-06
更新日期:2006-07-01 00:00:00
abstract::Previous examination of thin sections of L929 cells heavily infected with the Q fever Priscilla isolate by conventional transmission electron microscopy indicated that the rickettsiae resided within multiple vacuoles. The present study using high-voltage electron microscopy and three-dimensional reconstruction reveale...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.10.4485-4488.1993
更新日期:1993-10-01 00:00:00
abstract::Two enteropathogenic strains of Escherichia coli (EEC) differed from a nonenteropathogenic strain of E. coli (NEEC) in their association with porcine small intestinal epithelium. The EEC characteristically were found along villi from tip to base and contiguous to the brush border. They were not in crypts. In contrast,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.5.4.595-605.1972
更新日期:1972-04-01 00:00:00
abstract::Vibrio cholerae, the pathogen that causes cholera, also survives in aqueous reservoirs, probably in the form of biofilms. Quorum sensing negatively regulates V. cholerae biofilm formation through HapR, whose expression is induced at a high cell density. In this study, we show that the concentration of the quorum-sensi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01190-06
更新日期:2007-01-01 00:00:00
abstract::We investigated the effect of a pH 4-treated human immunoglobulin preparation for intravenous use (pH4-G) on the production of tumor necrosis factor alpha (TNF-alpha) in vivo. The level of TNF-alpha in the sera of rabbits receiving pH4-G before lipopolysaccharide (LPS) injection was lower than that in rabbits receivin...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.5.1384-1390.1990
更新日期:1990-05-01 00:00:00