Abstract:
:The rfb region from Vibrio cholerae O139 strain MO45 was cloned from cosmid gene banks established in Escherichia coli HB101, using an immunoblot assay for screening of the correct clones. Immunoblot analysis of lipopolysaccharide (LPS) preparations revealed the presence of two types of positive clones: (i) those expressing only a short core-linked O polysaccharide (SOPS) and (ii) those also expressing a highly polymerized capsular polysaccharide (CPS) not bound to the E. coli K-12 LPS core. In addition, the latter clones appear to contain a locus which may encode a putative regulator of SOPS and CPS chain length. Further characterization in E. coli showed that CPS constitutes a barrier against large particles such as the bacteriophage Ffm but not against bacteriophage lambda or P1. In addition, a portion of the K-12 LPS core may not be substituted with SOPS. Loci associated with the two clonal types were transferred into V. cholerae CH19, an rfbAB deletion mutant of CVD103-HgR deficient in the production of the homologous Inaba O polysaccharide. This resulted in the stable expression of SOPS, alone or together with CPS, that was indistinguishable from that of wild-type V. cholerae O139. Strains CH25 and CH26, which correspond to CH19 bearing the V. cholerae O139 rfb region integrated into the chromosome, were found to be genetically stable and essentially identical to the parent CVD103-HgR with respect to physiological properties such as cell motility, mercury resistance, toxicity, and production of the cholera toxin B subunit. Rabbits immunized with CH25 elicited high titers of anti-O139 SOPS- and CPS-specific serum antibodies. These strains possess characteristics desirable in candidate live oral vaccines against V. cholerae O139.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Favre D,Cryz SJ Jr,Viret JFdoi
10.1128/IAI.64.9.3565-3570.1996subject
Has Abstractpub_date
1996-09-01 00:00:00pages
3565-70issue
9eissn
0019-9567issn
1098-5522journal_volume
64pub_type
杂志文章abstract::The enterotoxin of Shigella dysenteriae I produces fluid and electrolyte secretion in the rabbit ileum. These present studies were designed to evaluate nonelectrolyte transport in rabbit ileal mucosa exposed to Shigella enterotoxin. Both 10 mM galactose and 5 mM L-alanine absorptions were significantly impaired in ent...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.2.510-512.1977
更新日期:1977-05-01 00:00:00
abstract::Mycoplasma genitalium is a sexually transmitted pathogen associated with several acute and chronic reproductive tract disease syndromes in men and women. To evaluate the suitability of a pig-tailed macaque model of M. genitalium infection, we inoculated a pilot animal with M. genitalium strain G37 in the uterine cervi...
journal_title:Infection and immunity
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doi:10.1128/IAI.01322-12
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1998-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.7.5.771-776.1973
更新日期:1973-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.53.2.331-338.1986
更新日期:1986-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.5.1826-1833.1992
更新日期:1992-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/IAI.74.5.3052-3059.2006
更新日期:2006-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.2.949-957.2004
更新日期:2004-02-01 00:00:00
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journal_title:Infection and immunity
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更新日期:2014-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.11.4716-4723.1993
更新日期:1993-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.32.2.840-851.1981
更新日期:1981-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章,评审
doi:10.1128/IAI.00443-12
更新日期:2012-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05511-11
更新日期:2012-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01117-06
更新日期:2007-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.4.1343-1350.1992
更新日期:1992-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.12.7089-7094.2002
更新日期:2002-12-01 00:00:00
abstract::We have previously shown that chinchillas infected with a multiply passaged laboratory strain of influenza A/NWS/33 (H1N1) develop negative middle-ear pressure; polymorphonuclear leukocyte oxidative, bactericidal, and chemotactic dysfunction; and increased susceptibility to pneumococcal otitis media. Because influenza...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.41.3.913-920.1983
更新日期:1983-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.2.685-691.2002
更新日期:2002-02-01 00:00:00
abstract::Attention has recently been focused on immunoglobulin A1 (IgA1) protease production as a possible virulence factor of bacteria implicated in meningitis and gonorrhea. This report demonstrates that suspected principal etiological agents in destructive periodontal disease include bacteria capable of degrading IgA1, IgA2...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.3.757-765.1981
更新日期:1981-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2006-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.3.961-967.1994
更新日期:1994-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-11-01 00:00:00
abstract::The role of antibody in protection against infection with Cryptococcus neoformans is undefined. In this paper we describe the development of opsonic activity in the cerebrospinal fluid (CSF) of rabbits in response to cryptococcal meningitis. The opsonin appeared to be immunoglobulin G (IgG); the activity was heat stab...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.7.2115-2119.1990
更新日期:1990-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.1.50-61.2005
更新日期:2005-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.7.4312-4318.2000
更新日期:2000-07-01 00:00:00
abstract::The presence of hepatitis B surface antigen (HBsAg) and antibody (anti-HBs), hepatitis B e antigen (HBeAg) and antibody (anti-HBe), the nature of T-cell function, and specific cell-mediated immunity to HBsAg were determined and evaluated serially in groups of subjects with chronic HBsAg carrier states and in seronegat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.2.480-488.1978
更新日期:1978-08-01 00:00:00
abstract::Active immunization with Porphyromonas gingivalis whole-cell preparations has been shown to prevent P. gingivalis infection and oral bone loss. Employing passive antibody transfer and opsonization, we demonstrate with this study that immunization-elicited P. gingivalis-specific immunoglobulin G facilitates clearance o...
journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2004-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.7.2688-2693.1992
更新日期:1992-07-01 00:00:00
abstract::We investigated the mechanisms that lead to the production of proinflammatory mediators by human monocytes when these cells are exposed in vitro to live Borrelia burgdorferi spirochetes. We first focused on myeloid differentiation primary response protein 88 (MyD88), an adapter molecule that is essential in the Toll-l...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01078-08
更新日期:2009-03-01 00:00:00
abstract::Pseudomonas aeruginosa, an important opportunistic pathogen of humans, exploits epithelial damage to establish infection. We have rigorously explored the role of N-glycoproteins and heparan sulfate proteoglycans (HSPGs) in P. aeruginosa-mediated attachment and subsequent downstream events at the apical (AP) and basola...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01215-09
更新日期:2010-03-01 00:00:00