Abstract:
:Cronobacter sakazakii is an opportunistic pathogen that causes neonatal meningitis and necrotizing enterocolitis. Its interaction with intestinal epithelium is important in the pathogenesis of enteric infections. In this study, we investigated the involvement of the inv gene in the virulence of C. sakazakii ATCC 29544 in vitro and in vivo. Sequence analysis of C. sakazakii ATCC 29544 inv revealed that it is different from other C. sakazakii isolates. In various cell culture models, an Δinv deletion mutant showed significantly lowered invasion efficiency, which was restored upon genetic complementation. Studying invasion potentials using tight-junction-disrupted Caco-2 cells suggested that the inv gene product mediates basolateral invasion of C. sakazakii ATCC 29544. In addition, comparison of invasion potentials of double mutant (ΔompA Δinv) and single mutants (ΔompA and Δinv) provided evidence for an additive effect of the two putative outer membrane proteins. Finally, the importance of inv and the additive effect of putative Inv and OmpA were also proven in an in vivo rat pup model. This report is the first to demonstrate two proteins working synergistically in vitro, as well as in vivo in C. sakazakii pathogenesis.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Chandrapala D,Kim K,Choi Y,Senevirathne A,Kang DH,Ryu S,Kim KPdoi
10.1128/IAI.01397-13subject
Has Abstractpub_date
2014-05-01 00:00:00pages
1755-65issue
5eissn
0019-9567issn
1098-5522pii
IAI.01397-13journal_volume
82pub_type
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pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
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doi:10.1128/IAI.56.1.13-17.1988
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更新日期:2010-11-01 00:00:00
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更新日期:2006-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.1.122-131.1983
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pub_type: 杂志文章
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更新日期:1987-11-01 00:00:00