Abstract:
:Changes in cell viability and in factors affecting metabolic integrity were examined after exposure of Escherichia coli LP1092 to human serum. Antibody-dependent classical pathway activity accounted for the rapid killing of strain LP1092 by complement. Removal of serum lysozyme by bentonite absorption or by neutralization with anti-human lysozyme immunoglobulin G resulted in a reduction in the rate of killing; optimal activity could be restored by the addition of physiological amounts of egg-white lysozyme. The pattern of 86Rb+ and alkaline phosphatase release obtained after serum treatment did not support the view that complement simultaneously disrupts cytoplasmic and outer membrane integrity. Macromolecular synthesis was affected late in the reaction sequence; complete inhibition of precursor incorporation into RNA, DNA, and protein occurred only after almost total loss of bacterial colony-forming ability. Addition of chloramphenicol, an inhibitor of protein synthesis, to the bactericidal system resulted in a marked reduction in the rate of serum killing. Killing was completely inhibited by an inhibitor (KCN) and an uncoupler (2,4-dinitrophenol) of oxidative phosphorylation. Exposure of LP1092 cells to serum was followed by a rapid and large increase in intracellular ATP levels; ATP synthesis did not occur when bacteria were exposed to dialyzed serum, which killed LP1092 cells at a much reduced rate. Addition of glucose or serum ultrafiltrate to dialyzed serum restored optimal bactericidal activity. We suggest that optimal killing of gram-negative bacteria is an energy-dependent process requiring an input of bacterially generated ATP.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Taylor PW,Kroll HPdoi
10.1128/IAI.39.1.122-131.1983subject
Has Abstractpub_date
1983-01-01 00:00:00pages
122-31issue
1eissn
0019-9567issn
1098-5522journal_volume
39pub_type
杂志文章abstract::In nature, mixed Borrelia burgdorferi infections are common and possibly can be acquired by either superinfection or coinfection. Superinfection by heterologous B. burgdorferi strains has been established experimentally, although the ability of homologous B. burgdorferi clones to superinfect a host has not been studie...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01817-14
更新日期:2014-11-01 00:00:00
abstract::We have previously shown that Bacillus anthracis lethal toxin represses glucocorticoid receptor (GR) transactivation. We now report that repression of GR activity also occurs with the large clostridial toxins produced by Clostridium sordellii and C. difficile. This was demonstrated using a transient transfection assay...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00291-07
更新日期:2007-08-01 00:00:00
abstract::In experimental Leishmania donovani infection in BALB/c mice, initial susceptibility gives way to T-cell-dependent acquired resistance and eventual control over visceral infection. Since various cytokines appear to underlie the host response to Leishmania infection, we examined infected liver tissue for gene expressio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.3.1058-1063.1994
更新日期:1994-03-01 00:00:00
abstract::A plaque assay system for pathogenic rickettsiae, which utilizes primary chick embryo tissue cultures, is described. It proved to be a highly reproducible measure of infectiousness for Rickettsia rickettsi and R. typhi, which were employed in most studies; as well as for R. canada, R. prowazeki, R. sibirica, R. akari,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.5.5.715-722.1972
更新日期:1972-05-01 00:00:00
abstract::Extracellular protease activity was measured in liquid cultures of representative strains from six serotypes of Legionella pneumophila. A variety of substrates were degraded, including denatured casein, skim milk, gelatin, and hide powder azure, but not elastin. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.1.299-302.1981
更新日期:1981-10-01 00:00:00
abstract::The antigenically conserved proteins of Treponema pallidum subsp. pallidum and four nonpathogenic cultivatable treponemes were investigated by phase partitioning with the nonionic detergent Triton X-114 and immunoblot analysis. None of the T. pallidum integral membrane proteins identified by phase partitioning (deterg...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.7.1825-1828.1988
更新日期:1988-07-01 00:00:00
abstract::In order to construct an effector strain for the replacement therapy of dental caries, we wished to combine the properties of low-level acid production and high-level colonization potential in a strain of Streptococcus mutans. To this end, we made a deletion in the lactate dehydrogenase (LDH) gene cloned from the bact...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.1.60-64.1994
更新日期:1994-01-01 00:00:00
abstract::The toxicity of Chlamydia trachomatis was studied with mouse peritoneal macrophage culture. Inoculation of 30 inclusion-forming units of trachoma B/TW-5/OT organisms and 250 inclusion-forming units of lymphogranuloma venereum L2/434/Bu organisms per cell caused immediated toxicity, with the killing of 40 to 90% of the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.3.613-618.1978
更新日期:1978-06-01 00:00:00
abstract::Pseudomonas aeruginosa elastase rapidly inactivates alpha 1-proteinase inhibitor by splitting its Pro-357-Met-358 peptide bond. The present study was aimed at testing whether this reaction takes place in the presence of leukocyte elastase. To this end was added alpha 1-proteinase inhibitor to a mixture of the two elas...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.12.3793-3797.1989
更新日期:1989-12-01 00:00:00
abstract::Genomic DNA fingerprint analysis was performed on 39 Staphylococcus aureus and 28 Enterococcus faecalis endophthalmitis isolates collected from multiple clinical centers. Among 21 S. aureus genomic DNA fingerprint patterns identified, five clonotypes were recovered from multiple unrelated patients and accounted for 58...
journal_title:Infection and immunity
pub_type: 杂志文章,多中心研究
doi:10.1128/IAI.66.1.356-360.1998
更新日期:1998-01-01 00:00:00
abstract::Although periodontal treatment by scaling and root planing (SCRP) is known to induce bacteremia, the effect of this procedure on the host immune response is not known. We have determined pre- and post-SCRP immunoglobulin G antibody titers to antigens of Actinobacillus actinomycetemcomitans in the sera of 22 patients w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.1.145-151.1994
更新日期:1994-01-01 00:00:00
abstract::Dendritic cell interactions with pathogenic microbes initiate and direct the development of subsequent adaptive responses. The protozoan pathogen Giardia lamblia infects the mammalian small intestine, leading to nutrient malabsorption and diarrhea but rarely causing inflammation. In order to begin to understand how th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00718-08
更新日期:2009-02-01 00:00:00
abstract::Pathogenic strains of Yersinia spp. inject a set of Yop effector proteins into eukaryotic cells by using a plasmid-encoded type III secretion system. In this study, we analyzed the inflammatory response of human umbilical vein endothelial cells (HUVECs) after infection with different Yersinia enterocolitica strains. W...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.7.3510-3520.2002
更新日期:2002-07-01 00:00:00
abstract::The mechanism by which Helicobacter pylori, which has little or no invasive activity, induces gastric-tissue inflammation and injury has not been well characterized. We have previously demonstrated that water-extracted proteins of H. pylori are capable of activating human monocytes by a lipopolysaccharide (LPS)-indepe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.4.1183-1187.1995
更新日期:1995-04-01 00:00:00
abstract::The cyst cell wall β-glucans of Pneumocystis have been shown to stimulate immune responses in lung epithelial cells, dendritic cells, and alveolar macrophages. Little is known about how the trophic life forms, which do not have a fungal cell wall, interact with these innate immune cells. Here, we report differences in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00519-16
更新日期:2016-11-01 00:00:00
abstract::Proteins were extracted at pH 2.5 from carefully washed brain homogenates of patients with subacute sclerosing panencephalitis. Immunoglobulin G was isolated from the extracts by gel filtration and immunoadsorbent chromatography and was 125I radioiodinated. The labeled immunoglobulin G was reacted with purified measle...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.1.202-210.1979
更新日期:1979-04-01 00:00:00
abstract::Chromosomes from Cryptococcus neoformans, an encapsulated yeast pathogen, were separated by contour-clamped homogeneous field gel electrophoresis. Seven strains representing all four serotypes were studied. It was found that each strain had a unique, reproducible pattern of chromosome bands which could potentially be ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.9.2624-2627.1989
更新日期:1989-09-01 00:00:00
abstract::Oligopeptides play important roles in bacterial nutrition and signaling. Using sequences from the available genome database for Mycobacterium tuberculosis H37Rv, the oligopeptide permease operon (oppBCDA) of Mycobacterium bovis BCG was cloned from a cosmid library. An opp mutant strain was constructed by homologous re...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.2.429-436.2000
更新日期:2000-02-01 00:00:00
abstract::We examined the effect of host age and health status on the adherence of mannose-sensitive piliated Escherichia coli K1 to human oral epithelial cells. Mannose-sensitive piliated bacteria adhered in comparable numbers to newborn, older infant, and adult cells (125 +/- 61, 198 +/- 54, and 139 +/- 69 bacteria per cell, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.1.299-301.1984
更新日期:1984-07-01 00:00:00
abstract::We have previously described (L. M. Vaughan, P. R. Smith, and T. J. Foster, Infect. Immun. 61:2172-2181, 1993) the construction of a kanamycin-resistant aromatic-dependent mutant of Aeromonas salmonicida, the causative agent of furunculosis, and its use as a live vaccine. Here we describe the construction of an unmark...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.9.3863-3869.1996
更新日期:1996-09-01 00:00:00
abstract::Salivary gland lysates of the sand fly Lutzomyia longipalpis markedly enhance the course of infection with Leishmania major in mice. Here we examine various parameters of this phenomenon. The exacerbative effect of L. longipalpis salivary gland lysates occurred in five different mouse strains; however, the character o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.5.1592-1598.1991
更新日期:1991-05-01 00:00:00
abstract::The effect of route of administration, dosage, and number of boosts employed during immunization with the polymyxin-release form of Escherichia coli heat-labile (LT) enterotoxin on the degree and duration of protection afforded was evaluated in rats which were challenged by the ligated loop technique. Increasing the b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.1.81-86.1980
更新日期:1980-01-01 00:00:00
abstract::Vibrio cholerae O1 has two biotypes, El Tor and Classical, and the latter is now presumed to be extinct in nature. Under carbohydrate-rich growth conditions, El Tor biotype strains produce the neutral fermentation end product 2,3-butanediol (2,3-BD), which prevents accumulation of organic acids from mixed acid ferment...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01237-10
更新日期:2011-08-01 00:00:00
abstract::Clinical trials of novel tuberculosis (TB) vaccines are expensive, while global resources for TB vaccine development are limited. Therefore, there is a need for robust and predictive preclinical data to support advancement of candidate vaccines into clinical trials. Here, we provide a rationale for using the nonhuman ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00776-17
更新日期:2018-01-22 00:00:00
abstract::Macrophages are among the most sensitive targets of bacterial endotoxin (LPS), responding to minute amounts of LPS by releasing a battery of inflammatory mediators. Transfection of macrophages with secretory leukocyte protease inhibitor (SLPI) renders these cells refractory to LPS stimulation. Here we show that uptake...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.9.4485-4489.1999
更新日期:1999-09-01 00:00:00
abstract::An "immunogenic" ribonucleic acid (Im-RNA) has been extracted from peritoneal exudate (PE) cells of rats that were immunized with sheep erythrocytes (SRBC). Following multiple phenol extractions and deoxyribonuclease treatment, the material obtained from PE cells was eluted from diethylaminoethyl-cellulose at 0.55 M N...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.8.2.215-221.1973
更新日期:1973-08-01 00:00:00
abstract::Glucan-binding protein B (GbpB) from Streptococcus mutans has been shown to induce protective immunity to dental caries in experimental models. Having recently sequenced the gbpB gene, our objective in this study was to identify immunogenic regions within the GbpB sequence for use in subunit vaccines. Potential region...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.3.1179-1184.2003
更新日期:2003-03-01 00:00:00
abstract::Oral spirochetes possess many potential virulence factors, including the capacity for tissue invasion and persistence despite a vigorous host immune response. In an attempt to identify treponemal immunoreactive components, sera derived from individuals with advanced periodontal disease were used as a reagent to isolat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.6.2041-2051.1997
更新日期:1997-06-01 00:00:00
abstract::The enteric pathogen Shigella is one of the leading causes of moderate-to-severe diarrhea and death in young children in developing countries. Transformed cell lines and animal models have been widely used to study Shigella pathogenesis. In addition to altered physiology, transformed cell lines are composed of a singl...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00740-18
更新日期:2019-03-25 00:00:00
abstract::Immunoglobulin class-specific antibodies were measured by a solid-phase radioimmunoassay in serum, bronchoalveolar washings (BAW), lung cell lysates, and spleen cell lysates in germfree mice after intranasal (i.n.) and intraperitoneal (i.p.) primary and secondary 10(5), 10(4), and 10(3) mean tissue culture infective d...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.17.3.521-527.1977
更新日期:1977-09-01 00:00:00