Abstract:
:We have shown that a member of the 70-kDa heat shock protein (Hsp70) family is a major target of the humoral immune response during Leishmania donovani infection. A recombinant fusion protein was recognized by sera from 92% (35 of 38) of patients with visceral leishmaniasis, including representatives from each of the major regions where it is endemic. Serological analysis of recombinant Hsp70, expressed by a series of deletion constructs, identified the carboxy-terminal region as the immunodominant site. This region, which is the most evolutionarily divergent part of the molecule, was recognized by all sera from patients with visceral leishmaniasis which exhibited an anti-Hsp70 response. Purified recombinant L. donovani Hsp70 was not recognized by sera from patients with cutaneous leishmaniasis, Chagas' disease, leprosy, malaria, or schistosomiasis. To determine the regions involved in antibody recognition, a series of overlapping peptides were synthesized on polyethylene pins by the Pepscan method, and a hexamer, EADDRA, was identified by the visceral leishmaniasis serum samples as an immunodominant B-cell epitope.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Wallace GR,Ball AE,MacFarlane J,el Safi SH,Miles MA,Kelly JMdoi
10.1128/IAI.60.7.2688-2693.1992keywords:
subject
Has Abstractpub_date
1992-07-01 00:00:00pages
2688-93issue
7eissn
0019-9567issn
1098-5522journal_volume
60pub_type
杂志文章abstract::Antibiotics suppressed development of aster yellows (AY) disease symptoms in plants of china aster [Callistephus chinensis (L.) Nees.] and annual chrysanthemum (Chrysanthemum carinatum, Schousb.). When inoculated chrysanthemum plants were treated by any of several techniques with tetracycline antibiotics or chloramphe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.2.2.201-208.1970
更新日期:1970-08-01 00:00:00
abstract::Burkholderia cepacia is an opportunistic pathogen that causes severe systemic infections in patients with chronic granulomatous disease (CGD) or with cystic fibrosis (CF), but its mechanisms of virulence are poorly understood. We developed a murine model of systemic infection in wild-type (WT) and gamma interferon kno...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.8.4027-4032.1999
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abstract::Naegleria fowleri produces fatal meningoencephalitis in humans and in experimentally infected laboratory animals. The course of the disease in mice is dependent upon the infecting dose of amoebae, route of inoculation, and prior exposure to Naegleria antigens. DUB/ICR mice were immunized by various routes and antigen ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.3.817-820.1977
更新日期:1977-06-01 00:00:00
abstract::Vaccination has been proposed for the prevention of disease due to enterohemorrhagic Escherichia coli (EHEC), but the immune response following human infection, including the choice of potential antigens, has not been well characterized. To study this, sera were obtained from five pediatric patients with acute diarrhe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.9.5090-5095.2000
更新日期:2000-09-01 00:00:00
abstract::Spiroplasmas were propagated in the Drosophila melanogaster cell line Dm-1. Spiroplasma citri and unidentified strains (corn shunt organism, 277F [tick isolate], powder puff, BNR-1, honey bee, and OBMG) grew to 10(8) to 10(9) colony-forming units per ml and could be passaged. Cytopathic effect (CPE) varied with the in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.1.296-304.1982
更新日期:1982-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.7.3894-3899.2000
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abstract::We previously demonstrated that plasmid-deficient Chlamydia muridarum retains the ability to infect the murine genital tract but does not elicit oviduct pathology because it fails to activate Toll-like receptor 2 (TLR2). We derived a plasmid-cured derivative of the human genital isolate Chlamydia trachomatis D/UW-3/Cx...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01118-10
更新日期:2011-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.6.1720-1729.1990
更新日期:1990-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.52.3.911-913.1986
更新日期:1986-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.2.1387-1393.2006
更新日期:2006-02-01 00:00:00
abstract::Alveolar macrophages are the effector cells largely responsible for clearance of Pneumocystis carinii from the lungs. Binding of organisms to beta-glucan and mannose receptors has been shown to stimulate phagocytosis of the organisms. To further define the mechanisms used by alveolar macrophages for clearance of P. ca...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00393-07
更新日期:2007-08-01 00:00:00
abstract::Cell-mediated immune (CMI) responses and tumor necrosis factor alpha (TNF-alpha) have been shown to be essential in acquired protection against Cryptococcus neoformans. Induction of a protective anticryptococcal CMI response includes increases in dendritic cells (DC) and activated CD4(+) T cells in draining lymph node...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.1.68-74.2003
更新日期:2003-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00782-08
更新日期:2008-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.10.6185-6189.2004
更新日期:2004-10-01 00:00:00
abstract::Numerous bacterial pathogens use type III secretion systems (T3SSs) or T4SSs to inject or translocate virulence proteins into eukaryotic cells. Several different reporter systems have been developed to measure the translocation of these proteins. In this study, a peptide tag-based reporter system was developed and use...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00690-06
更新日期:2006-10-01 00:00:00
abstract::Autotransporters of gram-negative bacteria are single-peptide secretion systems that consist of a functional N-terminal alpha-domain ("passenger") fused to a C-terminal beta-domain ("translocator"). How passenger proteins are translocated through the outer membrane has not been resolved, and at present essentially thr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.7.3851-3859.2005
更新日期:2005-07-01 00:00:00
abstract::Nonspecific cross-reacting antigen (NCA) is a well-characterized membrane glycoprotein on granulocytes, macrophages, and lung epithelium. Structural studies at the protein and genomic levels have revealed that NCA is a member of the immunoglobulin supergene family, and hybridization studies showed that the transcript ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.7.2485-2493.1991
更新日期:1991-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:2002-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.5.1409-1412.1989
更新日期:1989-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2011-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00958-13
更新日期:2014-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::We have previously described a new system for the delivery of recombinant antigens in live Salmonella vaccines as genetic fusions to the C terminus of fragment C of tetanus toxin (TetC) driven by the anaerobically inducible nirB promoter. It has been reported that preimmunization with tetanus toxoid (TT) can suppress ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.7.2564-2569.1995
更新日期:1995-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00091-18
更新日期:2018-11-20 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.6.2295-2299.1996
更新日期:1996-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.9.3267-3272.1991
更新日期:1991-09-01 00:00:00
abstract::Recombinant adenylate cyclase toxoids are shown to deliver inserted foreign CD4(+)-T-cell epitopes into the major histocompatibility complex class II presentation pathway, inducing a specific CD4(+)-T-cell response in vivo and yielding in vitro stimulation of specific CD4(+) T cells at a 100-times-higher molar efficie...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:2002-02-01 00:00:00
abstract::Most oral bacteria tested formed colonies on a chemically defined medium with a chromogenic arylsulfatase substrate. Arylsulfatase activity was, however, restricted to Campylobacter-Wolinella group organisms, including Wolinella recta, a possible periodontopathogen. W. recta was the only arylsulfatase-active species a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.5.1380-1383.1989
更新日期:1989-05-01 00:00:00
abstract::The mechanism of in vitro synergistic lysis of sheep erythrocytes by Corynebacterium ovis and Corynebacterium equi was investigated. Hemolysis required (i) the action of phospholipase D from C. ovis, (ii) the action of an extracellular protein of C. equi, and (iii) Mg2+. Maximum lysis required imposition on the system...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.29.1.123-131.1980
更新日期:1980-07-01 00:00:00