Interaction of Treponema denticola TD-4, GM-1, and MS25 with human gingival fibroblasts.

Abstract:

:The adherence of Treponema denticola GM-1, TD-4, and MS25 to human gingival fibroblasts (HGFs) was studied to serve as an introduction to investigations into the interactions of these oral bacteria with human host cells. Under both aerobic (5% CO2) and anaerobic (85% N2 plus 10% H2 plus 5% CO2) environments, the interactions with the HGFs were such that strains GM-1 and MS25 were consistently more adherent than strain TD-4. Polyclonal antibodies to GM-1 inhibited GM-1 adherence by 70%, while MS25 and TD-4 showed differing degrees of cross-reactive inhibition, indicative of common but not identical epitopes on the surface of the three T. denticola strains. Pretreatment of the three strains with trypsin did not inhibit adherence; proteinase K did, however, inhibit this interaction by 80%. Trypsin pretreatment of the HGFs resulted in increases in adherence of 50 and 86% for GM-1 and MS25, respectively, while a decrease of 41% was noted for TD-4. Exposure of the T. denticola strains to sugars and lectin pretreatment of the HGFs implicated adherence mediation by mannose and galactose residues on the HGF surface. Periodate treatment of HGFs resulted in a 50% drop in adherence for GM-1 and MS25, but did not decrease that of TD-4. Addition of fetal bovine serum inhibited adherence of the three strains to differing degrees, with TD-4 being the most susceptible. Addition of purified fibronectin (100 micrograms/ml) resulted in greater than 50% inhibition in GM-1 and MS25 adherence, while a 25% increase occurred with TD-4. While strain differences were noted in some of the parameters studied, the results indicate two possibilities for T. denticola-HGF adherence: a lectinlike adhesin(s) on the T. denticola surface with affinity for galactose and mannose on the HGF surface, and a serum host factor(s) bridging T. denticola and HGFs.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Weinberg A,Holt SC

doi

10.1128/IAI.58.6.1720-1729.1990

subject

Has Abstract

pub_date

1990-06-01 00:00:00

pages

1720-9

issue

6

eissn

0019-9567

issn

1098-5522

journal_volume

58

pub_type

杂志文章
  • Adenoviral delivery of interleukin-10 fails to attenuate experimental Lyme disease.

    abstract::Production of interleukin-10 (IL-10) by C57BL/6 mice following infection with Borrelia burgdorferi has been proposed as a mechanism whereby resistance to the development of experimental Lyme arthritis is maintained. In the current study, we sought to determine the role of IL-10 during infection of arthritis- and cardi...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00808-08

    authors: Brown CR,Lai AY,Callen ST,Blaho VA,Hughes JM,Mitchell WJ

    更新日期:2008-12-01 00:00:00

  • Expression of a Clostridium perfringens type IV pilin by Neisseria gonorrhoeae mediates adherence to muscle cells.

    abstract::Clostridium perfringens is an anaerobic, Gram-positive bacterium that causes a range of diseases in humans, including lethal gas gangrene. We have recently shown that strains of C. perfringens move across the surface of agar plates by a unique type IV pilus (TFP)-mediated social motility that had not been previously d...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00909-10

    authors: Rodgers K,Arvidson CG,Melville S

    更新日期:2011-08-01 00:00:00

  • Acylation of the lipooligosaccharide of Haemophilus influenzae and colonization: an htrB mutation diminishes the colonization of human airway epithelial cells.

    abstract::Haemophilus influenzae is a commensal and opportunistic pathogen of the human airways. A number of surface molecules contribute to colonization of the airways by H. influenzae, such as adhesins, including structures found in the lipooligosaccharide (LOS). A human bronchiolar xenograft model was employed to investigate...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.70.8.4661-4668.2002

    authors: Swords WE,Chance DL,Cohn LA,Shao J,Apicella MA,Smith AL

    更新日期:2002-08-01 00:00:00

  • Extracellular dextranase activity produced by human oral strains of the genus Bifidobacterium.

    abstract::Three strains of anaerobic, dextranase-producing, gram-positive, rod-shaped bacteria were isolated from human dental plaque associated with root carious lesions. The isolates produced a molar ratio of acetate to lactate from glucose fermentation ranging from 1.1 to 1.9. Each strain also produced fructose-6-phosphate p...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.42.2.716-720.1983

    authors: Kaster AG,Brown LR

    更新日期:1983-11-01 00:00:00

  • In vitro studies of interaction of rickettsia and macrophages: effect of ultraviolet light on Coxiella burnetii inactivation and macrophage enzymes.

    abstract::The inactivation of Coxiella burnetii in suspension or in cultures of guinea pig peritoneal macrophages by ultraviolet (UV) light was studied. The effect of UV treatment on the activity of macrophage organelle marker enzymes and their subsequent equilibration in linear sucrose gradients was also determined. It was sho...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.27.3.837-841.1980

    authors: Little JS,Kishimoto RA,Canonico PG

    更新日期:1980-03-01 00:00:00

  • Virulence of a Legionella anisa strain associated with Pontiac fever: an evaluation using protozoan, cell culture, and guinea pig models.

    abstract::Legionella anisa and the amoeba Hartmannella vermiformis were isolated from an indoor fountain implicated as the infectious reservoir in an outbreak of Pontiac fever. We evaluated the ability of this strain of L. anisa to multiply in cultures of an amoeba (H. vermiformis), a ciliated protozoan (Tetrahymena pyriformis)...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.58.9.3139-3142.1990

    authors: Fields BS,Barbaree JM,Sanden GN,Morrill WE

    更新日期:1990-09-01 00:00:00

  • Reconstitution of an active arginine deiminase pathway in Mycoplasma pneumoniae M129.

    abstract::Some species of the genus Mycoplasma code for the arginine deiminase pathway (ADI), which enables these bacteria to produce ATP from arginine by the successive reaction of three enzymes: arginine deiminase (ArcA), ornithine carbamoyltransferase (ArcB), and carbamate kinase (ArcC). It so far appears that independently ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00441-13

    authors: Rechnitzer H,Rottem S,Herrmann R

    更新日期:2013-10-01 00:00:00

  • Selective pressures and lipopolysaccharide subunits as determinants of resistance of clinical isolates of gram-negative bacilli to human serum.

    abstract::Differences in molecular composition of lipopolysaccharides (LPS) between serum-sensitive (S) clinical isolates of Escherichia coli and serum-resistant (R) clones derived by serial passage in serum were demonstrated to determine sensitivity or resistance to killing by normal human serum (NHS). LPS from R clones had a ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.55.2.320-328.1987

    authors: Porat R,Johns MA,McCabe WR

    更新日期:1987-02-01 00:00:00

  • Congo red binding phenotype is associated with hemin binding and increased infectivity of Shigella flexneri in the HeLa cell model.

    abstract::Wild-type isolates of Shigella flexneri bind the dye Congo red from solid media, thus producing red (Crb+) colonies. Mutants which fail to bind the dye produce white colonies (Crb-) and are avirulent in a variety of systems. In S. flexneri the ability to bind Congo red correlates with the ability to bind hemin and pro...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.55.6.1393-1398.1987

    authors: Daskaleros PA,Payne SM

    更新日期:1987-06-01 00:00:00

  • Mitogenic activities of synthetic Escherichia coli lipid A and a synthetic partial structure (tripalmitoyl pentapeptide) of E. coli lipoprotein.

    abstract::Synthetic Escherichia coli lipid A and synthetic S-[2,3-bis-(palmitoyloxy)propyl]-N-palmitoylpentapeptide (tripalmitoyl pentapeptide [TPP]), representing the mitogenically active principles of bacterial lipopolysaccharide (LPS) and lipoprotein, respectively, were compared for their mitogenic activities on splenocytes ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.56.5.1382-1384.1988

    authors: Brade L,Bessler WG,Brade H

    更新日期:1988-05-01 00:00:00

  • An Iron-Regulated Autolysin Remodels the Cell Wall To Facilitate Heme Acquisition in Staphylococcus lugdunensis.

    abstract::Bacteria alter their cell surface in response to changing environments, including those encountered upon invasion of a host during infection. One alteration that occurs in several Gram-positive pathogens is the presentation of cell wall-anchored components of the iron-regulated surface determinant (Isd) system, which ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00397-15

    authors: Farrand AJ,Haley KP,Lareau NM,Heilbronner S,McLean JA,Foster T,Skaar EP

    更新日期:2015-09-01 00:00:00

  • Interleukin 12 is produced in vivo during endotoxemia and stimulates synthesis of gamma interferon.

    abstract::Gamma interferon (IFN-gamma) is produced in response to circulating lipopolysaccharide (LPS) and contributes to the lethality of endotoxic shock. To address the cellular source of IFN-gamma production in vivo, T cells and B cells were magnetically purified from C57BL/6 mouse spleens 5 h following endotoxin injection. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.62.10.4244-4249.1994

    authors: Heinzel FP,Rerko RM,Ling P,Hakimi J,Schoenhaut DS

    更新日期:1994-10-01 00:00:00

  • The Type III Secretion System Effector SeoC of Salmonella enterica subsp. salamae and S. enterica subsp. arizonae ADP-Ribosylates Src and Inhibits Opsonophagocytosis.

    abstract::Salmonella species utilize type III secretion systems (T3SSs) to translocate effectors into the cytosol of mammalian host cells, subverting cell signaling and facilitating the onset of gastroenteritis. In this study, we compared a draft genome assembly of Salmonella enterica subsp. salamae strain 3588/07 against the g...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00704-16

    authors: Pollard DJ,Young JC,Covarelli V,Herrera-León S,Connor TR,Fookes M,Walker D,Echeita A,Thomson NR,Berger CN,Frankel G

    更新日期:2016-11-18 00:00:00

  • Role of the HefC efflux pump in Helicobacter pylori cholesterol-dependent resistance to ceragenins and bile salts.

    abstract::The human gastric pathogen Helicobacter pylori modifies host cholesterol via glycosylation and incorporates the glycosylated cholesterol into its membrane; however, the benefits of cholesterol to H. pylori are largely unknown. We speculated that cholesterol in the H. pylori membrane might alter the susceptibility of t...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00974-09

    authors: Trainor EA,Horton KE,Savage PB,Testerman TL,McGee DJ

    更新日期:2011-01-01 00:00:00

  • Rickettsial infectious antibody complexes: detection by antiglobulin plaque reduction technique.

    abstract::Rickettsia rickettsii and R. australis formed infectious antibody complexes when reacted with homologous convalescent serum. The addition of antiglobulin to these complexes showed neutralization of plaques proportional to the concentration of rickettsial immune serum. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.9.5.966-968.1974

    authors: Kenyon RH,McManus AT

    更新日期:1974-05-01 00:00:00

  • Pseudomonas aeruginosa isolates from patients with cystic fibrosis: a class of serum-sensitive, nontypable strains deficient in lipopolysaccharide O side chains.

    abstract::Twenty-six Pseudomonas aeruginosa strains from patients with cystic fibrosis were typed by the Fisher immunotyping scheme. Only 6 strains were agglutinated by a single typing serum, whereas 15 strains were agglutinated with more than one serum and 5 were not agglutinated by any serum. Neither the polyagglutinable nor ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.42.1.170-177.1983

    authors: Hancock RE,Mutharia LM,Chan L,Darveau RP,Speert DP,Pier GB

    更新日期:1983-10-01 00:00:00

  • The C-terminal domain of Salmonella enterica serovar typhimurium OmpA is an immunodominant antigen in mice but appears to be only partially exposed on the bacterial cell surface.

    abstract::We examined the way the major outer membrane protein OmpA of Salmonella enterica serovar Typhimurium is recognized by the mouse immune system, by raising a panel of 12 monoclonal antibodies (MAbs) against this protein. Interaction between OmpA and these MAbs is competitively inhibited with several-hundredfold dilution...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.71.7.3937-3946.2003

    authors: Singh SP,Williams YU,Miller S,Nikaido H

    更新日期:2003-07-01 00:00:00

  • T-Cell epitopes and human leukocyte antigen restriction elements of an immunodominant antigen of Blastomyces dermatitidis.

    abstract::Humans infected with the dimorphic fungus Blastomyces dermatitidis develop strong T-lymphocyte responses to WI-1, an immunodominant antigen that has been shown to elicit protective immunity in mice. In the present study, the T-cell epitopes of WI-1 and human leukocyte antigen (HLA) restricting elements that display th...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.68.2.502-510.2000

    authors: Chang WL,Audet RG,Aizenstein BD,Hogan LH,DeMars RI,Klein BS

    更新日期:2000-02-01 00:00:00

  • Characterization of lectinlike surface components on Capnocytophaga ochracea ATCC 33596 that mediate coaggregation with gram-positive oral bacteria.

    abstract::The interactions between Capnocytophaga ochracea ATCC 33596 and Streptococcus sanguis H1, Actinomyces naeslundii PK984, or Actinomyces israelii PK16 are dependent on specific recognitions between heat-sensitive adhesins on C. ochracea and heat-stable structures (probably carbohydrate-containing receptors) on the surfa...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.55.5.1198-1202.1987

    authors: Weiss EI,London J,Kolenbrander PE,Kagermeier AS,Andersen RN

    更新日期:1987-05-01 00:00:00

  • Gram-positive cell walls stimulate synthesis of tumor necrosis factor alpha and interleukin-6 by human monocytes.

    abstract::Purified cell walls representing a wide variety in teichoic acid and peptidoglycan structure prepared from eight different gram-positive bacterial species induced the production of tumor necrosis factor alpha (TNF-alpha) and interleukin-6 from human monocytes in the presence of 10% plasma or serum. Significant amounts...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.62.7.2715-2721.1994

    authors: Heumann D,Barras C,Severin A,Glauser MP,Tomasz A

    更新日期:1994-07-01 00:00:00

  • Identification and characterization of a surface-associated protein (Ssp) of Staphylococcus saprophyticus.

    abstract::A 95-kDa protein was isolated from Staphylococcus saprophyticus 7108 grown on dialysis membranes placed on the surface of brain heart infusion agar. Strain CCM883 did not produce this protein. Ultrathin sections revealed the presence of very thin, tuftlike, 50- to 75-nm-long structures on the surface of strain 7108, w...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.60.3.1055-1060.1992

    authors: Gatermann S,Kreft B,Marre R,Wanner G

    更新日期:1992-03-01 00:00:00

  • Natural and immune antibodies for Vibrio fetus in serum and secretions of cattle.

    abstract::Naturally occurring antibodies for Vibrio fetus with whole-cell and O antigen specificities were detected in sera of mature cattle by means of the agglutination and indirect immunofluorescence reactions. O antibodies occurred in each of 21 sera examined, and whole-cell antibodies, in lower concentrations, occurred in ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.5.5.728-733.1972

    authors: Duncan JR,Wilkie BN,Winter AJ

    更新日期:1972-05-01 00:00:00

  • Localization of electron-dense tracers during entry of Rickettsia tsutsugamushi into polymorphonuclear leukocytes.

    abstract::The invasion of Rickettsia tsutsugamushi, Gilliam strain, into guinea pig polymorphonuclear leukocytes (PMNs) and the localization and distribution of tracers were followed during the process by electron microscopy. The seven tracers used were: cationized ferritin, ferritin, thorium dioxide (ThO2), carbon particles, l...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.30.1.231-243.1980

    authors: Rikihisa Y,Ito S

    更新日期:1980-10-01 00:00:00

  • Relapsing fever spirochetes Borrelia recurrentis and B. duttonii acquire complement regulators C4b-binding protein and factor H.

    abstract::Relapsing fever is a rapidly progressive and severe septic disease caused by certain Borrelia spirochetes. The disease is divided into two forms, i.e., epidemic relapsing fever, caused by Borrelia recurrentis and transmitted by lice, and the endemic form, caused by several Borrelia species, such as B. duttonii, and tr...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00007-06

    authors: Meri T,Cutler SJ,Blom AM,Meri S,Jokiranta TS

    更新日期:2006-07-01 00:00:00

  • Antigenic relatedness of glucosyltransferase enzymes from streptococcus mutans.

    abstract::The antigenic relationship of glucosyltransferases (GTF) produced by different serotypes of Streptococcus mutans was studied by using a functional inhibition assay. Rat, rabbit, or hamster immune fluids, directed to cell-associated or supernatant-derived GTF, were tested against ammonium sulfate-precipitated culture s...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.15.1.91-103.1977

    authors: Smith DJ,Taubman MA

    更新日期:1977-01-01 00:00:00

  • The tick salivary protein Salp15 inhibits the killing of serum-sensitive Borrelia burgdorferi sensu lato isolates.

    abstract::Borrelia burgdorferi, the agent of Lyme disease, is transmitted by ticks. During transmission from the tick to the host, spirochetes are delivered with tick saliva, which contains the salivary protein Salp15. Salp15 has been shown to protect spirochetes against B. burgdorferi-specific antibodies. We now show that Salp...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00232-08

    authors: Schuijt TJ,Hovius JW,van Burgel ND,Ramamoorthi N,Fikrig E,van Dam AP

    更新日期:2008-07-01 00:00:00

  • In vitro response of human T cells to Pseudomonas aeruginosa.

    abstract::Pseudomonas aeruginosa is a gram-negative bacillus that is a major cause of morbidity and mortality in immunosuppressed patients, burn patients, and patients with cystic fibrosis. Although immunity to these bacteria has been associated with serum antibody, more recent evidence suggests that T-cell-mediated immunity ma...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.40.2.670-674.1983

    authors: Porwoll JM,Gebel HM,Rodey GE,Markham RB

    更新日期:1983-05-01 00:00:00

  • Organization of genes responsible for the production of mannose-resistant fimbriae of a uropathogenic Escherichia coli isolate.

    abstract::A group of insertion mutants was used to define the genes of plasmid pDC5 required for the expression of mannose-resistant fimbriae. Minicell experiments identified four polypeptides (71,000, 45,000, 27,000, and 17,000 daltons) concerned with fimbrial production, the smallest of these being the fimbrial subunit. The a...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.42.3.900-906.1983

    authors: Clegg S,Pierce JK

    更新日期:1983-12-01 00:00:00

  • Cellular pertussis vaccine containing a Bordetella pertussis strain that produces a nontoxic pertussis toxin molecule.

    abstract::Bordetella pertussis 165-9K/129G, which produces a nontoxic form of pertussis toxin (PT), was used to prepare a whole-cell diphtheria-tetanus-pertussis (DTP) vaccine. The in vivo potency and the serological response induced by this vaccine were comparable to those of the conventional DTP vaccine which contains active ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.60.3.1150-1155.1992

    authors: Marsili I,Pizza M,Giovannoni F,Volpini G,Bartalini M,Olivieri R,Rappuoli R,Nencioni L

    更新日期:1992-03-01 00:00:00

  • Secretory leukocyte protease inhibitor interferes with uptake of lipopolysaccharide by macrophages.

    abstract::Macrophages are among the most sensitive targets of bacterial endotoxin (LPS), responding to minute amounts of LPS by releasing a battery of inflammatory mediators. Transfection of macrophages with secretory leukocyte protease inhibitor (SLPI) renders these cells refractory to LPS stimulation. Here we show that uptake...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.67.9.4485-4489.1999

    authors: Ding A,Thieblemont N,Zhu J,Jin F,Zhang J,Wright S

    更新日期:1999-09-01 00:00:00