Abstract:
:Twenty-six Pseudomonas aeruginosa strains from patients with cystic fibrosis were typed by the Fisher immunotyping scheme. Only 6 strains were agglutinated by a single typing serum, whereas 15 strains were agglutinated with more than one serum and 5 were not agglutinated by any serum. Neither the polyagglutinable nor the nonagglutinable strains were typable by hemagglutination inhibition or immunodiffusion, suggesting that these polyagglutinable strains did not express multiple serotype antigens, but were instead being agglutinated by antibody to nonserotype determinants. Four typable isolates were resistant to pooled normal human serum, whereas the 12 polyagglutinable and nonagglutinable isolates studied were very sensitive to normal human serum. The outer membranes of 16 strains were isolated and characterized. The data suggested, in general, strong conservation of outer membrane protein patterns. Lipopolysaccharides (LPS) were purified by a new technique which allowed isolation of both rough and smooth LPS in high yields. Three of four typable, serum-resistant strains examined had amounts of smooth, O-antigen-containing LPS equivalent to our laboratory wild type, P. aeruginosa PAO1 strain H103. In contrast, 10 of 12 polyagglutinable or nonagglutinable, serum-sensitive strains had very little or no smooth, O-antigen-containing LPS, and the other two contained less smooth LPS than our wild-type strain H103. In agreement with this data, five independent, rough, LPS O-antigen-deficient mutants of strain H103 were nontypable and serum sensitive. We suggest that the LPS defects described here represent a significant new property of many P. aeruginosa strains associated with cystic fibrosis.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Hancock RE,Mutharia LM,Chan L,Darveau RP,Speert DP,Pier GBdoi
10.1128/IAI.42.1.170-177.1983subject
Has Abstractpub_date
1983-10-01 00:00:00pages
170-7issue
1eissn
0019-9567issn
1098-5522journal_volume
42pub_type
杂志文章abstract::A protein antigen called TR-o was isolated from supernatant of a sonically treated Reiter treponeme. The isolation procedure included anion-exchange chromatography on Whatman DE-52, hydrophobic interaction chromatography on decyl agarose, and finally gel filtration on Ac-A-22 Ultrogel. The fractionations were monitore...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.3.974-978.1982
更新日期:1982-03-01 00:00:00
abstract::We have developed an in vitro assay to study the intramacrophage replication of Mycobacterium tuberculosis or Mycobacterium bovis BCG. In this assay, extracellular mycobacterial replication was avoided by using a small infecting inoculum and a new method for efficiently removing nonphagocytocytized bacilli. The abilit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.2.786-793.1982
更新日期:1982-08-01 00:00:00
abstract::Immune responses of mice to Streptococcus mutans serotype c were analyzed by means of the enzyme-linked immunospot assay to determine the predominant specificities of the antibodies developed. In general, the numbers of splenic antibody-secreting cells correlated with serum antibody levels. A low dose (10(8) CFU) of k...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.53.2.317-323.1986
更新日期:1986-08-01 00:00:00
abstract::Exoantigens of Plasmodium vivax-parasitized erythrocytes stimulated macrophages to secrete tumor necrosis factor, and antisera raised against the exoantigens inhibited this secretion. The antisera also inhibited the activity of Plasmodium falciparum and Plasmodium yoelii exoantigens, and conversely, antisera against t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.3.1241-1243.1992
更新日期:1992-03-01 00:00:00
abstract::Candida albicans is a common opportunistic fungal pathogen and is the leading cause of invasive fungal diseases in immunocompromised individuals. The induction of cell-mediated immunity to C. albicans is one of the main tasks of cells of the innate immune system, and in vitro evidence suggests that integrin α(M)β₂ (CR...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00650-10
更新日期:2011-04-01 00:00:00
abstract::Athymic nude mice (nu/nu), heterozygous litter mates (nu/+), and thymic reconstituted homozygous animals (nu/nu TxR) were infected with Trypanosoma rhodesiense. A reduced parasitemia and prolonged survival were observed in the nu/nu animals after infection and cure or after immunization with irradiated organisms. Thes...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.3.714-720.1978
更新日期:1978-06-01 00:00:00
abstract::Ehrlichia chaffeensis is an obligately intracellular gram-negative bacterium and is the etiologic agent of human monocytic ehrlichiosis (HME). Although E. chaffeensis induces the generation of several cytokines and chemokines by leukocytes, E. chaffeensis lacks lipopolysaccharide and peptidoglycan. Bioinfomatic analys...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00056-08
更新日期:2008-08-01 00:00:00
abstract::Mice injected intravenously with a commercially available extract of Coxiella burnetii prepared for use as the antigen in the complement fixation diagnostic test for Q fever were subsequently resistant to infection with Babesia microti, Babesia rodhaini, and Plasmodium vinckei petteri. The parasites appeared to die in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.2.319-325.1979
更新日期:1979-05-01 00:00:00
abstract::The effect of continuous exposure to 80% oxygen on newborn mice with Ureaplasma urealyticum pneumonia was determined. Mice were inoculated intranasally with either U. urealyticum or sterile broth and then housed in either 80% oxygen or room air (21% oxygen). The mice were sacrificed at either 7 or 14 days after inocul...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.11.3487-3493.1990
更新日期:1990-11-01 00:00:00
abstract::[1-(14)C]acetate is incorporated into lipids more rapidly by liver from Aeromonas salmonicida-infected trout than by normal trout liver, both in vitro and in vivo. The increase was, in general, about twofold to less than threefold. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.14.3.836-838.1976
更新日期:1976-09-01 00:00:00
abstract::Hydrosalpinx induction in mice by Chlamydia muridarum infection, a model that has been used to study C. trachomatis pathogenesis in women, is known to depend on the cryptic plasmid that encodes eight genes designated pgp1 to pgp8. To identify the plasmid-encoded pathogenic determinants, we evaluated C. muridarum trans...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02576-14
更新日期:2014-12-01 00:00:00
abstract::Bovine tumor necrosis factor alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) cDNAs were generated by reverse transcription and then by PCR amplification from lipopolysaccharide (LPS)-stimulated alveolar macrophage RNA. The amplified cDNAs were cloned into pPow and expressed in Escherichia coli DH5 alpha. The expr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.2.381-388.1995
更新日期:1995-02-01 00:00:00
abstract::The biological response to endotoxin mediated through the Toll-like receptor 4 (TLR4)-MD-2 receptor complex is directly related to lipid A structure or configuration. Endotoxin structure may also influence activation of the MyD88-dependent and -independent signaling pathways of TLR4. To address this possibility, human...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.5.2940-2950.2005
更新日期:2005-05-01 00:00:00
abstract::Subcutaneous infection chambers in rabbits were infected with a strain of Staphylococcus aureus isolated from a patient with toxic shock syndrome. Estrogens (mestranol and 17-beta-estradiol) protected male rabbits and prolonged survival. Neither androgens (testosterone and dihydrotestosterone) nor progesterone affecte...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.52.1.331-333.1986
更新日期:1986-04-01 00:00:00
abstract::It is not clear how the host initially recognizes and responds to infection by gram-negative pathogenic Brucella spp. It was previously shown (D. S. Weiss, B. Raupach, K. Takeda, S. Akira, and A. Zychlinsky, J. Immunol. 172:4463-4469, 2004) that the early macrophage response against gram-negative bacteria is mediated ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.8.5137-5143.2005
更新日期:2005-08-01 00:00:00
abstract::Attempts were made to isolate an antigen(s) from Candida albicans suitable for detecting hypersensitivity in a murine model of candidiasis. Using footpad reactivity in cutaneously infected animals as the assay, comparisons were made of two commercial extracts and cell wall and cytoplasmic preparations made in the labo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.1.88-98.1978
更新日期:1978-04-01 00:00:00
abstract::Immunity to Mycobacterium tuberculosis is dependent upon the generation of a protective gamma interferon (IFN-gamma)-producing T-cell response. Recent studies have suggested that interleukin-6 (IL-6) is required for the induction of a protective T-cell response and that IL-4 may suppress the induction of IFN-gamma. To...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.6.3322-3326.2000
更新日期:2000-06-01 00:00:00
abstract::While several diseases associated with Chlamydia psittaci infection have been reported in Phascolarctos cinereus (koala), it is still unclear whether one or more chlamydial strains are responsible. In this study, we provide evidence, obtained by restriction enzyme and gene probe analysis, that two quite distinct strai...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.8.1897-1900.1988
更新日期:1988-08-01 00:00:00
abstract::Monoclonal antibodies have been generated to the unique distal sugar epitopes on the oligosaccharide haptens of the glycopeptidolipid antigens of clinically prominent members of the Mycobacterium avium serocomplex. Thus, antibodies are described that recognize the distal O-acetyl-alpha-L-rhamnopyranosyl residue of the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.10.3147-3158.1989
更新日期:1989-10-01 00:00:00
abstract::Campylobacter jejuni is a major cause of human inflammatory enteritis. During the course of human disease numerous proinflammatory cytokines are produced. Little is known, however, about the cytokine responses produced during the interaction of this bacterium with the avian host. Campylobacter has been considered a co...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.4.2094-2100.2005
更新日期:2005-04-01 00:00:00
abstract::C-reactive protein (CRP) has long been known to appear in the sera of individuals with inflammatory processes, but its role in host defense against bacterial infection is unclear. We have recently demonstrated that CRP in the presence of the classical complement pathway markedly enhances the opsonization of Streptococ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.3.987-992.1982
更新日期:1982-09-01 00:00:00
abstract::Antigen preparations derived from Plasmodium falciparum-infected erythrocytes (but not from uninfected erythrocytes) can stimulate the in vitro proliferation of peripheral blood lymphocytes from malaria-sensitized as well as nonsensitized donors. The possibility that the nonspecific responses might be due to a parasit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.1.106-110.1979
更新日期:1979-04-01 00:00:00
abstract::In bacterial infections, mononuclear and polymorphonuclear phagocytes are key components of host defenses. Recent investigations have indicated that chemokines are able to recruit and activate phagocytes. In particular, interleukin-8 (IL-8) attracts polymorphonuclear leukocytes (PMNs), while monocyte chemoattractant p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.9.4005-4014.1994
更新日期:1994-09-01 00:00:00
abstract::Interleukin-18 (IL-18) is a proinflammatory cytokine that plays an important role in natural killer cell activation and the T helper 1 (Th1) cell response, particularly in collaboration with IL-12. Since Th1 cells play a pivotal role in the host defense against infection with intracellular microbes, such as Leishmania...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.5.2449-2456.2000
更新日期:2000-05-01 00:00:00
abstract::Pneumonia due to Gram-negative bacteria is associated with high mortality. Acinetobacter baumannii is a Gram-negative bacterium that is associated with hospital-acquired and ventilator-associated pneumonia. Bacteria have been described to release outer membrane vesicles (OMVs) that are capable of mediating systemic in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00243-19
更新日期:2019-08-21 00:00:00
abstract::In malarial infections of primates, the spleen has been shown to modulate parasite antigen expression on the surfaces of infected erythrocytes. The processes affected include cytoadherence, which is central to the pathophysiology of severe falciparum malaria, and the related phenomenon of rosette formation. In this st...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.6.2225-2228.1992
更新日期:1992-06-01 00:00:00
abstract::Legionella pneumophila multiplied rapidly in guinea pig and rat alveolar macrophages but failed to grow when phagocytic activity was inhibited by pretreatment with 0.5 or 1.0 microgram of cytochalasin D per ml. Attachment was not inhibited by cytochalasin D. No extracellular multiplication occurred when L. pneumophila...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.51.1.31-36.1986
更新日期:1986-01-01 00:00:00
abstract::Among a number of laboratory strains of Listeria monocytogenes used in experimental infection, strain LO28 is highly capable of inducing robust beta interferon (IFN-β) production in infected macrophages. In this study, we investigated the molecular mechanism of the IFN-β-inducing ability of LO28 by comparing it with t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.06162-11
更新日期:2012-07-01 00:00:00
abstract::The structural gene of pneumolysin was randomly mutagenized. A screen, developed to identify mutants with reduced hemolytic activity, identified substitutions within the cysteine-containing region and also at residues toward the N terminus of the toxin. These are the first reported changes within the N-terminal region...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.2.757-758.1994
更新日期:1994-02-01 00:00:00
abstract::Vibrio cholerae O1 has two biotypes, El Tor and Classical, and the latter is now presumed to be extinct in nature. Under carbohydrate-rich growth conditions, El Tor biotype strains produce the neutral fermentation end product 2,3-butanediol (2,3-BD), which prevents accumulation of organic acids from mixed acid ferment...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01237-10
更新日期:2011-08-01 00:00:00