Abstract:
:The structural gene of pneumolysin was randomly mutagenized. A screen, developed to identify mutants with reduced hemolytic activity, identified substitutions within the cysteine-containing region and also at residues toward the N terminus of the toxin. These are the first reported changes within the N-terminal region of the toxin that affect cytolytic activity.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Hill J,Andrew PW,Mitchell TJdoi
10.1128/IAI.62.2.757-758.1994subject
Has Abstractpub_date
1994-02-01 00:00:00pages
757-8issue
2eissn
0019-9567issn
1098-5522journal_volume
62pub_type
杂志文章abstract::Peptidoglycans of Leptotrichia buccalis ATCC 14201 and ATCC 19616 were isolated by extraction with sodium dodecyl sulfate and subsequent digestion of the sodium dodecyl sulfate-insoluble residue with proteases and alpha-amylase. Cell wall fractions obtained by sodium dodecyl sulfate extraction and protease digestion w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.1.132-136.1983
更新日期:1983-01-01 00:00:00
abstract::A nasal vaccine, consisting of outer membrane vesicles (OMVs) from group B Neisseria meningitidis, was given to 12 volunteers in the form of nose drops or nasal spray four times at weekly intervals, with a fifth dose 5 months later. Each nasal dose consisted of 250 microg of protein, equivalent to 10 times the intramu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.4.1334-1341.1998
更新日期:1998-04-01 00:00:00
abstract::The skin microenvironment at the site of infection plays a role in the early events that determine protective T helper 1/type 1 immune responses during cutaneous leishmaniasis (CL) infection. During CL in nonhealing BALB/c mice, early interleukin-4 (IL-4) can instruct dendritic cells for protective Th1 immunity. Addit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00710-18
更新日期:2018-11-20 00:00:00
abstract::Corynebacterium renale type I (strain 115), 1.7 X 10(7) to 4.5 X 10(7) organisms, introduced intravenously into mice disappeared from the blood less than 24 h after inoculation and did not produce pyelonephritis. The same strain, 1 X 10(7) to 5 X 10(7) organisms, inoculated into the urinary bladder of mice was not rec...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.1.263-267.1977
更新日期:1977-04-01 00:00:00
abstract::Help from CD4 T cells is often important for the establishment of primary and memory CD8 T-cell responses. However, it has yet to be determined whether T helper polarization affects the delivery of help and/or whether responding CD8 T cells helped by Th1 or Th2 cells express distinct effector properties. To address th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01328-06
更新日期:2007-05-01 00:00:00
abstract::The kinetics of Bacillus anthracis toxin production in culture and its lethal activity in rats, mice, and guinea pigs were investigated. Lethal toxin activity was produced in vitro throughout exponential growth at essentially identical rates in both encapsulated virulent and nonencapsulated avirulent strains. The two ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.3.761-767.1984
更新日期:1984-09-01 00:00:00
abstract::Macrophages encode several Toll-like receptors (TLRs) that recognize bacterial components, such as lipoproteins (TLR2) or lipopolysaccharides (TLR4), and activate multiple signaling pathways. Activation of transcription factor NF-kappaB by TLR2 or TLR4 signaling promotes proinflammatory and cell survival responses. Al...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.3.1513-1519.2003
更新日期:2003-03-01 00:00:00
abstract::Peritoneal exudate T lymphocytes from Listeria monocytogenes-immune mice in the presence of the homologous antigen (heat-killed L. monocytogenes) and normal macrophages showed L. monocytogenes-specific proliferative responses. Proliferation was inhibited by macrophages from L. monocytogenes- or Corynebacterium parvum-...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.3.907-913.1982
更新日期:1982-12-01 00:00:00
abstract::Arg-gingipain (Rgp) and Lys-gingipain (Kgp) are Porphyromonas gingivalis cysteine proteinases implicated as major virulence factors in pathologies of periodontitis. We purified a 660-kDa cell-associated gingipain complex existing as a homodimer of two catalytically active monomers which comprises their catalytic and a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.2.883-893.2005
更新日期:2005-02-01 00:00:00
abstract::The efficacy of the innate immune system depends on its ability to mount an appropriate response to diverse infections and damaging agents. Key components of this system are pattern recognition receptors that detect pathogen-associated and damage-associated molecular patterns (PAMPs and DAMPs). Nlrp1b is a pattern rec...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01003-12
更新日期:2013-02-01 00:00:00
abstract::We have previously described a new system for the delivery of recombinant antigens in live Salmonella vaccines as genetic fusions to the C terminus of fragment C of tetanus toxin (TetC) driven by the anaerobically inducible nirB promoter. It has been reported that preimmunization with tetanus toxoid (TT) can suppress ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.7.2564-2569.1995
更新日期:1995-07-01 00:00:00
abstract::Peroral immunization with a live strain of Escherichia coli O6K13H1 against experimental ascending pyelonephritis caused by the same strain was studied in rats, and the effect of immunization on antibody titers against the O and K antigens and lipid A was determined. Peroral immunization with live bacteria protected s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.2.647-653.1982
更新日期:1982-02-01 00:00:00
abstract::It has been shown previously that serum from chronically infected patients with cystic fibrosis inhibits the phagocytosis of Pseudomonas aeruginosa by both normal and cystic fibrosis alveolar macrophages. In the present study, the ability of peripheral monocytes and polymorphonuclear leukocytes from normal volunteers ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.2.802-805.1982
更新日期:1982-11-01 00:00:00
abstract::Severe combined immunodeficient beige mice were inoculated orally and intraperitoneally with a bovine strain of Mycobacterium paratuberculosis to explore their potential as laboratory animal models in the study of paratuberculosis (Johne's disease). Control animals were similarly inoculated with heat-killed M. paratub...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.10.4074-4079.1992
更新日期:1992-10-01 00:00:00
abstract::The serum opacity factor (SOF) of Streptococcus pyogenes is a serotyping tool and pathogenesis factor. Using SOF-coated latex beads in cell adherence assays and antiserum directed against SOF in S. pyogenes-HEp-2 cell adherence inhibition experiments, we demonstrate SOF involvement in the fibronectin-mediated adherenc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.7.4302-4308.2004
更新日期:2004-07-01 00:00:00
abstract::Tick saliva has potent immunomodulatory properties. In arthropod-borne diseases, this effect is largely used by microorganisms to increase their pathogenicity and to evade host immune responses. We show that in Lyme borreliosis, tick salivary gland extract and a tick saliva protein, Salp15, inhibit in vitro keratinocy...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00482-10
更新日期:2011-02-01 00:00:00
abstract::N-Acetyl-muramyl-L-alanyl-D-isoglutamine (MDP), a structurally defined fragment of bacterial peptidoglycan, stimulated significant release of previously incorporated 45Ca from fetal rat bones in tissue culture over the concentration range of 0.1 to 10.0 micrograms/ml. MDP-Stimulated bone resorption was not inhibited b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.1.133-137.1982
更新日期:1982-01-01 00:00:00
abstract::We have focused on regions of staphylococcal enterotoxin C1 (SEC1) causing immunomodulation. N-terminal deletion mutants lacking residues 6 through 13 induced T-cell proliferation similar to that induced by native toxin. However, mutants with residues deleted between positions 19 and 33, although nonmitogenic themselv...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.8.3396-3407.1994
更新日期:1994-08-01 00:00:00
abstract::Toll-like receptors 2 and 4 (TLR2 and TLR4) have been found to transduce signals of peptidoglycan (PGN) and lipopolysaccharide (LPS), respectively, for NF-kappa B activation. However, little is known about the expression and regulation of the TLR2 gene in monocytes/macrophages in response to the two typical bacterial ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.5.2788-2796.2001
更新日期:2001-05-01 00:00:00
abstract::An asd-stabilized plasmid carrying enterotoxigenic Escherichia coli cfaABCE genes was constructed and called pJGX15C-asd+. Expression of colonization factor antigen I (CFA/I) by this plasmid occurs independently of the cfaABCE positive regulator cfaR in attenuated Salmonella delta aro delta asd strain H683 and nonpath...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.12.4933-4938.1995
更新日期:1995-12-01 00:00:00
abstract::An antigenically distinct serotype, type IV, has recently been added to the recognized serotypes of group B streptococci (GBS). We isolated and purified the capsular polysaccharide antigen from a prototype type IV GBS strain. The type IV capsular polysaccharide formed a precipitin line with rabbit antiserum to type IV...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.4.1089-1094.1989
更新日期:1989-04-01 00:00:00
abstract::Toxoplasma gondii is an obligate intracellular parasite that resides in the cytoplasm of its host in a unique membrane-bound vacuole known as the parasitophorous vacuole (PV). The membrane surrounding the parasite is remodeled by the dense granules, secretory organelles that release an array of proteins into the vacuo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00782-08
更新日期:2008-11-01 00:00:00
abstract::Pertussis is a severe respiratory disease caused by Bordetella pertussis The classic symptoms of pertussis include paroxysmal coughing with an inspiratory whoop, posttussive vomiting, cyanosis, and persistent coryzal symptoms. Infants under 2 months of age experience more severe disease, with most deaths occurring in ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00511-18
更新日期:2018-10-25 00:00:00
abstract::Natural-resistance-associated macrophage protein 1 (Nramp1) is a divalent cation transporter belonging to a family of transporter proteins highly conserved in eukaryotes and prokaryotes. Mammalian and bacterial transporters may compete for essential metal ions during mycobacterial infections. The mycobacterial Nramp h...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.8.4124-4131.2002
更新日期:2002-08-01 00:00:00
abstract::Several strains of group B Streptococcus agalactiae were found to be lethal for young adult rats. When bacteria were heat killed and then injected intraperitoneally into rats, rapid death (14 to 18 h) of the rats occurred, characterized by labored breathing, hemolyzed serum, hemoglobinuria, and subungual hemorrhages. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.48.2.560-564.1985
更新日期:1985-05-01 00:00:00
abstract::Fibronectin binds to Streptococcus pyogenes, and this binding is inhibited by lipoteichoic acid (LTA). Previous studies have shown that LTA can be released from S. pyogenes by treatment with penicillin. Penicillin released LTA from both S. pyogenes and Staphylococcus aureus; however, the binding of fibronectin correla...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.51.2.529-535.1986
更新日期:1986-02-01 00:00:00
abstract::The nitrate dissimilation pathway is important for anaerobic growth in Pseudomonas aeruginosa. In addition, this pathway contributes to P. aeruginosa virulence by using the nematode Caenorhabditis elegans as a model host, as well as biofilm formation and motility. We used a set of nitrate dissimilation pathway mutants...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00822-09
更新日期:2009-10-01 00:00:00
abstract::Monoclonal antibodies to Trichomonas vaginalis were prepared by immunizing mice with a cloned isolate of T. vaginalis. Eight antibodies reacted with the same four isolates or strains but did not react with the other T. vaginalis strains or isolates tested. All eight antibodies reacted uniformly with both the body and ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.1.270-275.1984
更新日期:1984-01-01 00:00:00
abstract::The need for agents designed to modify immune response in the treatment of patients with viral infection, immunodeficiency, or cancer prompted the present study on the mechanisms of action of isoprinosine, a compound developed for antiviral use and whose therapeutic activity may involve the immune system. The effect o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.2.382-387.1976
更新日期:1976-02-01 00:00:00
abstract::The cytokine response to the agent of human granulocytic ehrlichiosis (HGE) was assessed in a murine infection model and the role of gamma interferon (IFN-gamma), a cytokine that is crucial for host defenses against intracellular pathogens, was investigated by using IFN-gamma-deficient mice. The agent of HGE (aoHGE) i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.4.1827-1833.2000
更新日期:2000-04-01 00:00:00