Isolation and characterization of type IV group B Streptococcus capsular polysaccharide.

Abstract:

:An antigenically distinct serotype, type IV, has recently been added to the recognized serotypes of group B streptococci (GBS). We isolated and purified the capsular polysaccharide antigen from a prototype type IV GBS strain. The type IV capsular polysaccharide formed a precipitin line with rabbit antiserum to type IV GBS organisms but not with antiserum to organisms of GBS serotype Ia, Ib, II, or III. Enzyme-linked immunosorbent assay inhibition experiments showed no cross-reaction between type IV antiserum and other GBS serotypes. Capsular polysaccharide released from the bacterial cells with mutanolysin and that isolated from the culture supernatant had similar elution profiles on Sepharose CL-6B, with a Kav of 0.30 and an estimated Mr of 200,000. The purified type IV polysaccharide was found to contain galactose, glucose, N-acetylglucosamine, and N-acetylneuraminic acid (sialic acid) as exclusive sugars. The polysaccharide contained 23% (by weight) sialic acid and galactose, glucose, and N-acetylglucosamine in a relative ratio of (1):1.10:0.55. These results are compatible with a repeating structure of six monosaccharide residues containing galactose, glucose, N-acetylglucosamine, and sialic acid in a molar ratio of 2:2:1:1. Unlike type Ia, II, and III GBS polysaccharides, desialylation of the type IV polysaccharide produced an antigen which formed a line of identity with the native type IV antigen in double diffusion in agar against homologous antiserum. This result suggests that sialic acid is not as critical to the immunodeterminant structure of the type IV antigen as it is for other GBS capsular types.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Wessels MR,Benedí WJ,Jennings HJ,Michon F,DiFabio JL,Kasper DL

doi

10.1128/IAI.57.4.1089-1094.1989

subject

Has Abstract

pub_date

1989-04-01 00:00:00

pages

1089-94

issue

4

eissn

0019-9567

issn

1098-5522

journal_volume

57

pub_type

杂志文章
  • Innate immune response to Streptococcus iniae infection in zebrafish larvae.

    abstract::Streptococcus iniae causes systemic infection characterized by meningitis and sepsis. Here, we report a larval zebrafish model of S. iniae infection. Injection of wild-type S. iniae into the otic vesicle induced a lethal infection by 24 h postinfection. In contrast, an S. iniae mutant deficient in polysaccharide capsu...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00642-12

    authors: Harvie EA,Green JM,Neely MN,Huttenlocher A

    更新日期:2013-01-01 00:00:00

  • T-cell-independent stimulation of immunoglobulin secretion in resting human B lymphocytes by the mannose-specific adhesin of Escherichia coli type 1 fimbriae.

    abstract::Purified Escherichia coli type 1 fimbriae have been shown previously to stimulate T-cell-independent proliferation of human B lymphocytes. The response is mediated by the mannose-specific, lectin-like adhesin protein FimH. Here we show that type 1 fimbriae also stimulate immunoglobulin (Ig) secretion by B cells. The r...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.60.12.5197-5203.1992

    authors: Ponniah S,Abraham SN,Endres RO

    更新日期:1992-12-01 00:00:00

  • Localization of the cleavage site specificity determinant of Haemophilus influenzae immunoglobulin A1 protease genes.

    abstract::Immunoglobulin A1 (IgA1) proteases are produced by a number of different species of bacteria which cause infection at human mucosal surfaces. The sole substrate of these proteases is human IgA1. Cleavage is within the hinge region of IgA1, although there is variability in the exact peptide bond within the hinge region...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.58.2.320-331.1990

    authors: Grundy FJ,Plaut AG,Wright A

    更新日期:1990-02-01 00:00:00

  • Enhanced disease severity in Helicobacter pylori-infected mice deficient in Fas signaling.

    abstract::Recent evidence suggests that immune-mediated gastric epithelial cell apoptosis through Fas-Fas ligand interactions participates in Helicobacter pylori disease pathogenesis. To define the role of Fas signaling in vivo, H. pylori strain SS1 infection in C57BL/6 mice was compared to that in mice deficient in the Fas lig...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.70.5.2591-2597.2002

    authors: Jones NL,Day AS,Jennings H,Shannon PT,Galindo-Mata E,Sherman PM

    更新日期:2002-05-01 00:00:00

  • Binding of Candida albicans to immobilized amino acids and bovine serum albumin.

    abstract::In this study, we examined the binding of Candida albicans synchronized yeast-phase cells to plastic, immobilized amino acids and bovine serum albumin (BSA) and quantified the binding by using an XTT tetrazolium salt assay and absorbance determination. Our results show that C. albicans binds efficiently and specifical...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.66.1.140-144.1998

    authors: Hawser SP,Islam K

    更新日期:1998-01-01 00:00:00

  • Effects of age, ambient temperature, and heat-stable Escherichia coli enterotoxin on intestinal transit in infant mice.

    abstract::Some interrelationships among age, ambient temperature, intestinal transit, and enterotoxigenic Escherichia coli infection were studied in an infant mouse model. The transit of dye in the small intestine was accelerated during the response to heat-stable E. coli enterotoxin. Transit in the small intestine of normal mi...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.25.1.127-132.1979

    authors: Moon HW,Fung PY,Isaacson RE,Booth GD

    更新日期:1979-07-01 00:00:00

  • Antigenic polypeptide complex from the Melvin strain of Neisseria gonorrhoeae: isolation and properties.

    abstract::An antigenic complex has been isolated in a highly purified from from the Melvin strain of Neisseria gonorrhoeae. The complex has a molecular weight of 9.3 x 10(6) and on sodium dodecyl sulfate-polyacrylamide gel electrophoresis was found to consist of several subunits; the most predominant had the following molecular...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.25.2.635-644.1979

    authors: Karkhanis YD,Anderson RL,Zeltner JY,Carlo DJ,Stoudt TH

    更新日期:1979-08-01 00:00:00

  • Microbial amyloids induce interleukin 17A (IL-17A) and IL-22 responses via Toll-like receptor 2 activation in the intestinal mucosa.

    abstract::The Toll-like receptor 2 (TLR2)/TLR1 receptor complex responds to amyloid fibrils, a common component of biofilm material produced by members of the phyla Firmicutes, Bacteroidetes, and Proteobacteria. To determine whether this TLR2/TLR1 ligand stimulates inflammatory responses when bacteria enter intestinal tissue, w...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00911-12

    authors: Nishimori JH,Newman TN,Oppong GO,Rapsinski GJ,Yen JH,Biesecker SG,Wilson RP,Butler BP,Winter MG,Tsolis RM,Ganea D,Tükel Ç

    更新日期:2012-12-01 00:00:00

  • Burkholderia cenocepacia ShvR-regulated genes that influence colony morphology, biofilm formation, and virulence.

    abstract::Burkholderia cenocepacia is an opportunistic pathogen that primarily infects cystic fibrosis (CF) patients. Previously, we reported that ShvR, a LysR regulator, influences colony morphology, virulence, and biofilm formation and regulates the expression of an adjacent 24-kb genomic region encoding 24 genes. In this stu...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00170-11

    authors: Subramoni S,Nguyen DT,Sokol PA

    更新日期:2011-08-01 00:00:00

  • Resistance of calves to Cryptosporidium parvum: effects of age and previous exposure.

    abstract::Cryptosporidium parvum is a coccidian parasite that causes diarrheal disease in many vertebrate species, including young (less than or equal to 1 month old) calves. Older calves and adult cattle are resistant to infection. In this study, newborn calves were raised in isolation from C. parvum for 1 week to 3 months bef...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.58.7.2237-2240.1990

    authors: Harp JA,Woodmansee DB,Moon HW

    更新日期:1990-07-01 00:00:00

  • A regulator of Aspergillus fumigatus extracellular proteolytic activity is dispensable for virulence.

    abstract::Virulence of the fungal pathogen Aspergillus fumigatus is in part based on the saprophytic lifestyle that this mold has evolved. A crucial function for saprophytism resides in secreted proteases that allow assimilation of proteinaceous substrates. The impact of extracellular proteolytic activities on the pathogenesis ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00425-09

    authors: Bergmann A,Hartmann T,Cairns T,Bignell EM,Krappmann S

    更新日期:2009-09-01 00:00:00

  • Carbohydrate-binding sites of the mannose-specific fimbrial lectins of enterobacteria.

    abstract::The combining sites of type 1 fimbrial lectins of various species of enterobacteria were studied by measuring the inhibitory activity of linear and branched oligosaccharides and several glycosides of D-mannose on the agglutination of yeast cells by the organisms. The results showed that all five strains of Escherichia...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.43.3.1088-1090.1984

    authors: Firon N,Ofek I,Sharon N

    更新日期:1984-03-01 00:00:00

  • Immunochemical analysis of streptococcal group A, B, and C carbohydrates, with emphasis on group A.

    abstract::Streptococcal group A, B, and C carbohydrates were analyzed by counterimmunoelectrophoresis, immunoelectrophoresis, and inhibition of immunoprecipitation. Extracts of streptococci group A or C were shown by counterimmunoelectrophoresis to contain both anodic and cathodic migrating components. In immunoelectrophoresis,...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.37.1.209-215.1982

    authors: Fung JC,Wicher K,McCarty M

    更新日期:1982-07-01 00:00:00

  • Molecular characterization of the pathogen-specific, 34-kilodalton membrane immunogen of Treponema pallidum.

    abstract::The 34-kilodalton (kDa) antigen of Treponema pallidum subsp. pallidum (T. pallidum) is a pathogen-specific integral membrane protein. DNA sequence analysis of the cloned gene revealed an open reading frame encoding a primary product of 204 residues with a molecular mass of 22,087 daltons. Sequences that correspond to ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.57.11.3314-3323.1989

    authors: Swancutt MA,Riley BS,Radolf JD,Norgard MV

    更新日期:1989-11-01 00:00:00

  • Nonspecific resistance against infection with Salmonella typhi and Salmonella typhimurium induced in mice by cord factor (trehalose-6,6'-dimycolate) and its analogues.

    abstract::Mice pretreated intraperitoneally with trehalose-6,6'-dimycolate (cord factor) were protected against an intraperitoneal challenge with Salmonella typhi strain Ty2 or Salmonella typhimurium strain SR 11. The nonspecific resistance to S. typhi and S. typhimurium was still detectable 7 and 14 days, respectively, after a...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.14.5.1125-1129.1976

    authors: Yarkoni E,Bekierkunst A

    更新日期:1976-11-01 00:00:00

  • A heptosyltransferase mutant of Pasteurella multocida produces a truncated lipopolysaccharide structure and is attenuated in virulence.

    abstract::Pasteurella multocida is the causative agent of fowl cholera in birds. In a previous study using signature-tagged mutagenesis, we identified a mutant, AL251, which was attenuated for virulence in mice and in the natural chicken host. Sequence analysis indicated that AL251 had an insertional inactivation of the gene wa...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.72.6.3436-3443.2004

    authors: Harper M,Cox AD,St Michael F,Wilkie IW,Boyce JD,Adler B

    更新日期:2004-06-01 00:00:00

  • Induction of specific T-cell responses, opsonizing antibodies, and protection against Plasmodium chabaudi adami infection in mice vaccinated with genomic expression libraries expressed in targeted and secretory DNA vectors.

    abstract::It has been proposed that a multivalent malaria vaccine is necessary to mimic the naturally acquired resistance to this disease observed in humans. A major experimental challenge is to identify the optimal components to be used in such a multivalent vaccine. Expression library immunization (ELI) is a method for screen...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.71.8.4506-4515.2003

    authors: Rainczuk A,Scorza T,Smooker PM,Spithill TW

    更新日期:2003-08-01 00:00:00

  • Lipopolysaccharide serotyping of Neisseria meningitidis by hemagglutination inhibition.

    abstract::The method of hemagglutination inhibition was used to investigate the antigenic diversity of lipopolysaccharide (LPS) from Neisseria meningitidis and to develop a serotyping systems based on this antigen. The system uses outer membrane complex prepared by a simple extraction procedure to inhibit homologous hemagglutin...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.16.2.471-475.1977

    authors: Mandrell RE,Zollinger WD

    更新日期:1977-05-01 00:00:00

  • Gene knockout mice establish a primary protective role for major histocompatibility complex class II-restricted responses in Chlamydia trachomatis genital tract infection.

    abstract::Mice with disrupted beta 2-microglobulin (beta 2m-/-), I-A (class II-/-), or CD4 (CD4-/-) genes were examined for their capacity to resolve Chlamydia trachomatis genital tract infection. C57BL/6 and beta 2m-/- mice resolved infection similarly and were culture negative by 4 to 5 weeks following infection. Conversely, ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.63.12.4661-4668.1995

    authors: Morrison RP,Feilzer K,Tumas DB

    更新日期:1995-12-01 00:00:00

  • Disruption of an internal membrane-spanning region in Shiga toxin 1 reduces cytotoxicity.

    abstract::Shiga toxin type 1 (Stx1) belongs to the Shiga family of bipartite AB toxins that inactivate eukaryotic 60S ribosomes. The A subunit of Stxs are N-glycosidases that share structural and functional features in their catalytic center and in an internal hydrophobic region that shows strong transmembrane propensity. Both ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.66.11.5252-5259.1998

    authors: Suhan ML,Hovde CJ

    更新日期:1998-11-01 00:00:00

  • Immune response to Campylobacter jejuni and Campylobacter coli in a cohort of children from birth to 2 years of age.

    abstract::A cohort of 111 children from Bangui, Central African Republic, was surveyed for enteric Campylobacter infections from birth to the age of 2 years; stools were examined biweekly in these children until 6 months of age and at least four times per year thereafter until 2 years of age and after each diarrheal episode. Bl...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.57.8.2542-2546.1989

    authors: Martin PM,Mathiot J,Ipero J,Kirimat M,Georges AJ,Georges-Courbot MC

    更新日期:1989-08-01 00:00:00

  • Purification and characterization of Vibrio metschnikovii cytolysin.

    abstract::An extracellular cytolysin produced by Vibrio metschnikovii was purified by acid precipitation, phenyl-Sepharose CL-4B chromatography, and rechromatography on a phenyl-Sepharose CL-4B column and high-performance liquid chromatography on a Mono Q (anion-exchange) column. The purified cytolysin had a molecular weight of...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.56.4.954-960.1988

    authors: Miyake M,Honda T,Miwatani T

    更新日期:1988-04-01 00:00:00

  • Experimental syphilis and serological examination for treponematosis in hares.

    abstract::Of 202 captive hares studied, many of which had lesions on their external genital organs or testicular atrophy or both, 27% had positive serological tests for syphilis although dark-field examination of extracts of atrophic testes was negative. A total of 12 hares that were nonreactive for the serological test for syp...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.27.1.231-234.1980

    authors: Horvath I,Kemenes F,Molnar L,Szeky A,Racz I

    更新日期:1980-01-01 00:00:00

  • Reactivity profile of human anti-82-kilodalton rhoptry protein antibodies generated during natural infection with Plasmodium falciparum.

    abstract::Immunization of monkeys with the 82-kDa rhoptry protein (p82) of Plasmodium falciparum can protect them against a lethal blood stage challenge, and monoclonal antibodies to p82 inhibit parasite growth in vitro. The role that a p82-specific immune response might play in human immunity to the parasite is not known. To d...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.61.7.2960-2965.1993

    authors: Howard RF,Jensen JB,Franklin HL

    更新日期:1993-07-01 00:00:00

  • Infection with Chlamydia trachomatis alters the tyrosine phosphorylation and/or localization of several host cell proteins including cortactin.

    abstract::Infection of epithelial cells by two biovars of Chlamydia trachomatis results in the tyrosine phosphorylation of several host proteins. The most prominent change in host protein tyrosine phosphorylation involves a complex of proteins with molecular masses of 75 to 85 kDa (pp75/85) and 100 kDa (pp100). The C. trachomat...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.65.12.5301-5308.1997

    authors: Fawaz FS,van Ooij C,Homola E,Mutka SC,Engel JN

    更新日期:1997-12-01 00:00:00

  • The lpf gene cluster for long polar fimbriae is not involved in adherence of enteropathogenic Escherichia coli or virulence of Citrobacter rodentium.

    abstract::Using the enteropathogenic Escherichia coli (EPEC) genome sequence, we found that EPEC E2348/69 has an lpfABCDE gene cluster homologous (about 60% identical at the protein level) to the Salmonella long polar fimbria (LPF) operon. To determine whether this operon is essential for adherence, the lpfABCD(E2)(3) genes wer...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.74.1.265-272.2006

    authors: Tatsuno I,Mundy R,Frankel G,Chong Y,Phillips AD,Torres AG,Kaper JB

    更新日期:2006-01-01 00:00:00

  • Monoclonal antibodies against Vibrio vulnificus RtxA1 elicit protective immunity through distinct mechanisms.

    abstract::Vibrio vulnificus causes rapidly progressing septicemia with an extremely high mortality rate (≥50%), even with aggressive antibiotic treatment. The bacteria secrete multifunctional autoprocessing repeats-in-toxin (MARTX) toxins, which are involved in the pathogenesis of Gram-negative Vibrio species. Recently, we repo...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.02130-14

    authors: Lee TH,Cha SS,Lee CS,Rhee JH,Chung KM

    更新日期:2014-11-01 00:00:00

  • Identification of an outer membrane protein involved in utilization of hemoglobin-haptoglobin complexes by nontypeable Haemophilus influenzae.

    abstract::A recombinant plasmid containing a 6.5-kb fragment of nontypeable Haemophilus influenzae (NTHI) chromosomal DNA was shown to confer a hemoglobin-haptoglobin-binding phenotype on Escherichia coli. Use of a mini-Tn10kan transposon for random insertion mutagenesis of this recombinant plasmid allowed localization of the N...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.64.9.3703-3712.1996

    authors: Maciver I,Latimer JL,Liem HH,Muller-Eberhard U,Hrkal Z,Hansen EJ

    更新日期:1996-09-01 00:00:00

  • Rhoptry-associated protein 1-binding monoclonal antibody raised against a heterologous peptide sequence inhibits Plasmodium falciparum growth in vitro.

    abstract::Monoclonal antibodies (MAbs) specific for Plasmodium falciparum rhoptry-associated protein 1 (RAP-1) were generated and tested for inhibition of parasite growth in vitro. The majority of indirect immunofluorescence assay (IFA)-positive MAbs raised against recombinant RAP-1 positions 23 to 711 (rRAP-1(23-711)) recogniz...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.69.4.2558-2568.2001

    authors: Moreno R,Pöltl-Frank F,Stüber D,Matile H,Mutz M,Weiss NA,Pluschke G

    更新日期:2001-04-01 00:00:00

  • Use of auxotyping for epidemiological studies of Campylobacter jejuni and Campylobacter coli infections.

    abstract::A chemically defined medium developed for Neisseria gonorrhoeae was modified to support the growth of Campylobacter jejuni and Campylobacter coli. A total of 76 isolates of C. jejuni and 14 isolates of C. coli were tested on this medium, which was designated Campylobacter defined medium (CDM), over a 3-month period. A...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.48.2.384-388.1985

    authors: Tenover FC,Knapp JS,Patton C,Plorde JJ

    更新日期:1985-05-01 00:00:00