Abstract:
:Immunization of monkeys with the 82-kDa rhoptry protein (p82) of Plasmodium falciparum can protect them against a lethal blood stage challenge, and monoclonal antibodies to p82 inhibit parasite growth in vitro. The role that a p82-specific immune response might play in human immunity to the parasite is not known. To determine to what extent humans produce antibodies to p82 following infection with P. falciparum, sera from individuals believed to be hyperimmune, semi-immune, or never infected with the parasite were examined. Portions of the p82 gene were expressed separately as fusion proteins and used on immunoblots to test for antibodies to the recombinant proteins. All but 1 of the 30 immune sera possessed antibodies to p82, while nonimmune sera produced, at best, only a marginal signal to the fusion proteins. The signal intensity produced with the human immune sera depended on the region of p82 being assayed, with the N-terminal 37% of p82 producing stronger signals than more C-terminal parts of p82. This N-terminal domain contains a tandem octapeptide repeat (consensus KSSSPSXT/V) of the structure (repeat)2-Q-T-S-G-S/L-(repeat)3. It is shown here that the sequence of this repetitive motif is conserved among four parasite isolates at both the nucleotide and amino acid levels; the five-residue repeat interruption peptide QTSGS/L separating the two sets of repeats contains the only amino acid substitution (Ser or Leu) detected in this region to date. Despite their conservation of structure, the repeats do not appear to be the only epitope recognized by the human antibodies, since sera which recognize the N-terminal fusion protein containing the repeats also bind a related protein after truncation and removal of the repeats. These results indicate that the structurally conserved p82 molecule contains multiple B-cell epitopes and is likely to be immunogenic in most individuals during natural infections with P. falciparum. These observations are consistent with the idea that antibodies to p82 generated during parasite infection have a role in the development of immunity to the organism.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Howard RF,Jensen JB,Franklin HLdoi
10.1128/IAI.61.7.2960-2965.1993subject
Has Abstractpub_date
1993-07-01 00:00:00pages
2960-5issue
7eissn
0019-9567issn
1098-5522journal_volume
61pub_type
杂志文章abstract::Thirteen Habs-serotype strains could be classified into 11 groups depending on the characteristic patterns of 2-amino sugar composition; strains of serotypes 2, 5, and 7 had the same pattern, and each other serotype strain had its own distinctive pattern. In our classification, fucosamine and quinovosamine were of imp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.3.692-697.1977
更新日期:1977-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
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doi:10.1128/IAI.60.2.406-415.1992
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.53.2.331-338.1986
更新日期:1986-08-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01246-13
更新日期:2014-01-01 00:00:00
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journal_title:Infection and immunity
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更新日期:2017-07-19 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.1.15-18.1979
更新日期:1979-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00533-10
更新日期:2010-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.1.64-70.1993
更新日期:1993-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.7.2687-2694.1994
更新日期:1994-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01063-15
更新日期:2015-12-14 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.36.3.885-892.1982
更新日期:1982-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00162-13
更新日期:2013-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.9.5331-5339.2004
更新日期:2004-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.5.2602-2607.2000
更新日期:2000-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.12.4606-4609.1991
更新日期:1991-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.10.4407-4409.1992
更新日期:1992-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.4.5.581-588.1971
更新日期:1971-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.69.9.5313-5317.2001
更新日期:2001-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2001-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00348-19
更新日期:2019-07-23 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2003-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.10.4392-4397.1993
更新日期:1993-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.29.1.278-280.1980
更新日期:1980-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-03-01 00:00:00
abstract::Humoral and cellular immune responses to several antigens were compared in control and hypercholesterolemic groups of monkeys. Chronic hypercholesterolemia, with concomitant hyperphospholipidemia and hypotriglyceridemia, was produced experimentally by feeding monkeys a high-fat, high-cholesterol diet. When studied pri...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.8.1.105-109.1973
更新日期:1973-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.12.5157-5164.1997
更新日期:1997-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.12.7933-7936.2001
更新日期:2001-12-01 00:00:00