Abstract:
:Campylobacter jejuni is a microaerobic bacterium that produces an acute, self-limiting, watery or bloody diarrhea in humans. Little is known about how C. jejuni causes disease or even what specific capabilities it requires for survival in vivo. The enzyme, superoxide dismutase (SOD), which catalyzes the breakdown of superoxide radicals to hydrogen peroxide and dioxygen is one of the bacterial cell's major defense mechanisms against oxidative damage. A PCR-based search for sod genes in C. jejuni 81-176 revealed that this bacterium contained at least one sod gene. We cloned and sequenced a sod gene from 81-176 and determined that its predicted protein product was most similar to that of FeSODs (sodB genes). Transcriptional analysis indicated that this gene is monocistronic and may be transcribed from a sigma 70-like promoter. Nondenaturing polyacrylamide gels stained to reveal SOD activities, accompanied by inhibition studies, demonstrated that C. jejuni produces five electrophoretically distinct bands of SOD activity, all of which appeared to be FeSODs. Analysis of an 81-176 sodB strain revealed that all of these FeSOD activities may be products of the one sodB gene that we cloned. The expression and enzymatic activity of the respective sodB and FeSOD produced by both C. jejuni and Helicobacter pylori were examined in Escherichia coli. Both genes were expressed in E. coli, and the proteins produced were enzymatically active. Finally, the ability of the 81-176 sodB strain to survive INT407 cell invasion was found to be significantly decreased (12-fold) compared with that of the parent, suggesting a potential role for SodB in C. jejuni intracellular survival.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Pesci EC,Cottle DL,Pickett CLdoi
10.1128/IAI.62.7.2687-2694.1994subject
Has Abstractpub_date
1994-07-01 00:00:00pages
2687-94issue
7eissn
0019-9567issn
1098-5522journal_volume
62pub_type
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doi:10.1128/IAI.38.2.802-805.1982
更新日期:1982-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.1.263-267.1977
更新日期:1977-04-01 00:00:00
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journal_title:Infection and immunity
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更新日期:2000-01-01 00:00:00
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doi:10.1128/IAI.23.3.706-710.1979
更新日期:1979-03-01 00:00:00
abstract::Stenotrophomonas maltophilia is a ubiquitous bacterium and an emerging nosocomial pathogen. This bacterium is resistant to many antibiotics, associated with a number of infections, and a significant health risk, especially for immunocompromised patients. Given that Caenorhabditis elegans shares many conserved genetic ...
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更新日期:2015-12-07 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.5.1848-1854.1998
更新日期:1998-05-01 00:00:00
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更新日期:2017-04-21 00:00:00
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doi:10.1128/IAI.22.1.5-9.1978
更新日期:1978-10-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1981-03-01 00:00:00
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journal_title:Infection and immunity
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doi:
更新日期:1980-11-01 00:00:00
abstract::We have reported that P388D1 cell line murine macrophages stimulated with lipopolysaccharide (LPS) from Actinobacillus actinomycetemcomitans release interleukin-1 (IL-1) inhibitor. The IL-1 inhibitor was purified from conditioned media of P388D1 cells stimulated with A. actinomycetemcomitans LPS for 72 h to homogeneit...
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