Toll-like receptor 6 plays an important role in host innate resistance to Brucella abortus infection in mice.

Abstract:

:Brucella abortus is recognized by several Toll-like receptor (TLR)-associated pathways triggering proinflammatory responses that affect both the nature and intensity of the immune response. Previously, we demonstrated that B. abortus-mediated dendritic cell (DC) maturation and control of infection are dependent on the adaptor molecule MyD88. However, the involvement of all TLRs in response to B. abortus infection is not completely understood. Therefore, we decided to evaluate the requirement for TLR6 in host resistance to B. abortus. Here, we demonstrated that TLR6 is an important component for triggering an innate immune response against B. abortus. An in vitro luciferase assay indicated that TLR6 cooperates with TLR2 to sense Brucella and further activates NF-κB signaling. However, in vivo analysis showed that TLR6, not TLR2, is required for the efficient control of B. abortus infection. Additionally, B. abortus-infected dendritic cells require TLR6 to induce tumor necrosis factor alpha (TNF-α) and interleukin-12 (IL-12). Furthermore, our findings demonstrated that the mitogen-activated protein kinase (MAPK) signaling pathway is impaired in TLR2, TLR6, and TLR2/6 knockout (KO) DCs when infected with B. abortus, which may account for the lower proinflammatory cytokine production observed in TLR6 KO mouse dendritic cells. In summary, the results presented here indicate that TLR6 is required to trigger innate immune responses against B. abortus in vivo and is required for the full activation of DCs to induce robust proinflammatory cytokine production.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

de Almeida LA,Macedo GC,Marinho FA,Gomes MT,Corsetti PP,Silva AM,Cassataro J,Giambartolomei GH,Oliveira SC

doi

10.1128/IAI.01356-12

subject

Has Abstract

pub_date

2013-05-01 00:00:00

pages

1654-62

issue

5

eissn

0019-9567

issn

1098-5522

pii

IAI.01356-12

journal_volume

81

pub_type

杂志文章
  • Virulence of broad- and narrow-host-range Salmonella enterica serovars in the streptomycin-pretreated mouse model.

    abstract::Salmonella enterica subspecies I serovars are common bacterial pathogens causing diseases ranging from enterocolitis to systemic infections. Some serovars are adapted to specific hosts, whereas others have a broad host range. The molecular mechanisms defining the virulence characteristics and the host range of a given...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.74.1.632-644.2006

    authors: Suar M,Jantsch J,Hapfelmeier S,Kremer M,Stallmach T,Barrow PA,Hardt WD

    更新日期:2006-01-01 00:00:00

  • CapG(-/-) mice have specific host defense defects that render them more susceptible than CapG(+/+) mice to Listeria monocytogenes infection but not to Salmonella enterica serovar Typhimurium infection.

    abstract::Loss of the actin filament capping protein CapG has no apparent effect on the phenotype of mice maintained under sterile conditions; however, bone marrow-derived macrophages from CapG(-/-) mice exhibited distinct motility defects. We examined the ability of CapG(-/-) mice to clear two intracellular bacteria, Listeria ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.71.11.6582-6590.2003

    authors: Parikh SS,Litherland SA,Clare-Salzler MJ,Li W,Gulig PA,Southwick FS

    更新日期:2003-11-01 00:00:00

  • Phospholipase A activity associated with the growth of Rickettsia prowazekii in L929 cells.

    abstract::Cultured L929 cells infected with Rickettsia prowazekii had a greatly increased rate of hydrolysis of fatty acid from the oleic acid-radiolabeled phospholipids of the host cell membranes. The incorporation of fatty acid into phospholipid in an infected cell was only moderately inhibited relative to a mock-infected cel...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.57.1.36-40.1989

    authors: Winkler HH,Daugherty RM

    更新日期:1989-01-01 00:00:00

  • Identification of lambda gt11 clones encoding the major antigenic determinants expressed by Theileria parva sporozoites.

    abstract::An antiserum, C16, was raised in cattle against freeze-thawed extracts of sporozoites of Theileria parva (Muguga). This antiserum, which neutralizes sporozoite infectivity in vitro, identified theileria-specific antigens having approximate molecular masses of 105, 90, 85, 69, 67, 52, 47, and 43 kilodaltons (kDa) on We...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.58.6.1828-1834.1990

    authors: Iams KP,Hall R,Webster P,Musoke AJ

    更新日期:1990-06-01 00:00:00

  • Filifactor alocis Promotes Neutrophil Degranulation and Chemotactic Activity.

    abstract::Filifactor alocis is a recently recognized periodontal pathogen; however, little is known regarding its interactions with the immune system. As the first-responder phagocytic cells, neutrophils are recruited in large numbers to the periodontal pocket, where they play a crucial role in the innate defense of the periodo...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00496-16

    authors: Armstrong CL,Miralda I,Neff AC,Tian S,Vashishta A,Perez L,Le J,Lamont RJ,Uriarte SM

    更新日期:2016-11-18 00:00:00

  • Protection of mice from lethal endotoxemia by use of an ornithine-containing lipid or a serine-containing lipid.

    abstract::The effects of an ornithine-containing lipid [alpha-N-(3-acyloxyacyl)-ornithine (Orn-L)] or a serine-containing lipid [alpha-N-(3-acyloxyacyl)-serine (Ser-L)] from Flavobacterium meningosepticum on lethal endotoxemia in mice were examined. When 500 micrograms of Orn-L was intravenously administered 1 h before intraven...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.59.8.2560-2566.1991

    authors: Kawai Y,Kaneda K,Morisawa Y,Akagawa K

    更新日期:1991-08-01 00:00:00

  • Complement activation by whole endotoxin is blocked by a monoclonal antibody to factor B.

    abstract::Sepsis is both a common and, despite present-day therapy, a serious disease. The pathophysiology of the septic response is a complex, multifactorial phenomenon which in part involves the activation of complement by bacterial endotoxin. A monoclonal antibody to human complement factor B, code-named 1H5, which was capab...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.62.10.4549-4555.1994

    authors: Clardy CW

    更新日期:1994-10-01 00:00:00

  • Discordant Brucella melitensis antigens yield cognate CD8+ T cells in vivo.

    abstract::Brucella spp. are intracellular bacteria that cause the most frequent zoonosis in the world. Although recent work has advanced the field of Brucella vaccine development, there remains no safe human vaccine. In order to produce a safe and effective human vaccine, the immune response to Brucella spp. requires greater un...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00994-09

    authors: Durward MA,Harms J,Magnani DM,Eskra L,Splitter GA

    更新日期:2010-01-01 00:00:00

  • Rapid protection of gnotobiotic pigs against experimental salmonellosis following induction of polymorphonuclear leukocytes by avirulent Salmonella enterica.

    abstract::Oral inoculation of 5-day-old gnotobiotic pigs with Salmonella enterica serovar Typhimurium strain F98 resulted in severe enteritis and invasive disease. Preinoculation 24 h earlier with an avirulent mutant of Salmonella enterica serovar Infantis (1326/28) completely prevented disease for up to 14 days (when the exper...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.71.4.2182-2191.2003

    authors: Foster N,Lovell MA,Marston KL,Hulme SD,Frost AJ,Bland P,Barrow PA

    更新日期:2003-04-01 00:00:00

  • Calprotectin expression in vitro by oral epithelial cells confers resistance to infection by Porphyromonas gingivalis.

    abstract::Calprotectin, an S100 calcium-binding protein with broad-spectrum antimicrobial activity in vitro, is expressed in neutrophils, monocytes, and gingival keratinocytes. In periodontitis, calprotectin appears upregulated and is detected at higher levels in gingival crevicular fluid and tissue specimens. How calprotectin ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.69.7.4242-4247.2001

    authors: Nisapakultorn K,Ross KF,Herzberg MC

    更新日期:2001-07-01 00:00:00

  • Multiplication of Mycobacterium tuberculosis Within Normal and "Immune" Mouse Macrophages Cultivated With and Without Streptomycin.

    abstract::Acquired cellular immunity to infection with Mycobacterium tuberculosis is believed to reside in the capacity of mononuclear phagocytes of immunized animals to inhibit intracellular multiplication of the parasite. However, in macrophage tissue culture systems, it has been customary to employ streptomycin in the medium...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:

    authors: Patterson RJ,Youmans GP

    更新日期:1970-01-01 00:00:00

  • Streptococcus suis interactions with the murine macrophage cell line J774: adhesion and cytotoxicity.

    abstract::Streptococcus suis capsular type 2 is an important etiological agent of swine meningitis, and it is also a zoonotic agent. Since one hypothesis of the pathogenesis of S. suis infection is that bacteria enter the bloodstream and invade the meninges and other tissues in close association with mononuclear phagocytes, the...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.70.8.4312-4322.2002

    authors: Segura M,Gottschalk M

    更新日期:2002-08-01 00:00:00

  • Roles of peripheral leukocytes and tissue macrophages in antibacterial resistance induced by free or liposome-encapsulated muramyl tripeptide phosphatidylethanolamide.

    abstract::Administration of free muramyl tripeptide phosphatidylethanolamide (MTPPE) or liposome-encapsulated MTPPE (LE-MTPPE) in a twofold-lower dose at 24 h before bacterial inoculation resulted in clearance of intravenously inoculated Klebsiella pneumoniae by tissue macrophages, whereas in control mice, bacteria were not eff...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.60.11.4891-4897.1992

    authors: Melissen PM,van Vianen W,Bakker-Woudenberg IA

    更新日期:1992-11-01 00:00:00

  • Guanylate Binding Proteins Regulate Inflammasome Activation in Response to Hyperinjected Yersinia Translocon Components.

    abstract::Gram-negative bacterial pathogens utilize virulence-associated secretion systems to inject, or translocate, effector proteins into host cells to manipulate cellular processes and promote bacterial replication. However, translocated bacterial products are sensed by nucleotide binding domain and leucine-rich repeat-cont...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00778-16

    authors: Zwack EE,Feeley EM,Burton AR,Hu B,Yamamoto M,Kanneganti TD,Bliska JB,Coers J,Brodsky IE

    更新日期:2017-09-20 00:00:00

  • Effects of local immunization with glucosyltransferase on colonization of hamsters by Streptococcus mutans.

    abstract::Experiments were performed to study the effect of antibody to Streptococcus mutans glucosyltransferase (GTF) on the implantation of these organisms in hamsters. Salivary (immunoglobulin A) and serum (immunoglobulin G) antibodies to GTF and GTF-inhibiting activity were elicited by injection of GTF in Freund complete ad...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.37.2.656-661.1982

    authors: Smith DJ,Taubman MA,Ebersole JL

    更新日期:1982-08-01 00:00:00

  • Location of feline leukemia-sarcoma group-specific antigen in infected human tissue culture cells.

    abstract::Feline leukemia-sarcoma group-specific antigens were located in human embryo cells infected with feline leukemia and feline sarcoma viruses. This was done by using the fluorescent-antibody and enzyme-labeled antibody techniques at both light and electron microscopic levels. The antigens were found to be exclusively in...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.5.3.400-405.1972

    authors: Ubertini TR

    更新日期:1972-03-01 00:00:00

  • Burkholderia (basonym Pseudomonas) cepacia binding to lipid receptors.

    abstract::Piliated Burkholderia (formerly Pseudomonas) cepacia from sputa of cys tic fibrosis patients in Toronto, Canada, were shown earlier to bind to purified mucins and to a protein receptor on epithelial cells via a 22-kDa adhesin located on unique cable pili. However, a second receptor, thought to be lipid in nature, was ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.64.4.1420-1425.1996

    authors: Sylvester FA,Sajjan US,Forstner JF

    更新日期:1996-04-01 00:00:00

  • Capsule reduces adherence of enterotoxigenic Escherichia coli to isolated intestinal epithelial cells of pigs.

    abstract::Previous reports have demonstrated that heat-stable (A-type) capsule on piliated enterotoxigenic Escherichia coli enhances colonization of enterotoxigenic E. coli in the small intestine and enhances virulence of enterotoxigenic E. coli. In this report, four encapsulated enterotoxigenic E. coli strains and one encapsul...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.45.3.737-740.1984

    authors: Runnels PL,Moon HW

    更新日期:1984-09-01 00:00:00

  • FMS-like tyrosine kinase 3 ligand treatment of mice aggravates acute lung injury in response to Streptococcus pneumoniae: role of pneumolysin.

    abstract::FMS-like tyrosine kinase-3 ligand (Flt3L) is a dendritic cell (DC) growth and differentiation factor with potential in antitumor therapies and antibacterial immunization strategies. However, the effect of systemic Flt3L treatment on lung-protective immunity against bacterial infection is incompletely defined. Here, we...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00854-12

    authors: Brumshagen C,Maus R,Bischof A,Ueberberg B,Bohling J,Osterholzer JJ,Ogunniyi AD,Paton JC,Welte T,Maus UA

    更新日期:2012-12-01 00:00:00

  • Immunoglobulin A from bronchopulmonary secretions blocks bactericidal and opsonizing effects of antibody to nontypable Haemophilus influenzae.

    abstract::Patients with chronic bronchitis are colonized by and may develop acute bronchopulmonary infection due to nontypable Haemophilus influenzae (NTHI) despite the presence of bactericidal and opsonizing antibody to the infecting organism. To test the hypothesis that secretory immunoglobulin A (IgA) interferes with host de...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.45.1.36-40.1984

    authors: Musher DM,Goree A,Baughn RE,Birdsall HH

    更新日期:1984-07-01 00:00:00

  • MyD88-dependent responses involving toll-like receptor 2 are important for protection and clearance of Legionella pneumophila in a mouse model of Legionnaires' disease.

    abstract::Legionella pneumophila is a gram-negative facultative intracellular parasite of macrophages. Although L. pneumophila is the causative agent of a severe pneumonia known as Legionnaires' disease, it is likely that most infections caused by this organism are cleared by the host innate immune system. It is predicted that ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.02049-05

    authors: Archer KA,Roy CR

    更新日期:2006-06-01 00:00:00

  • Comparative genomics of Esx genes from clinical isolates of Mycobacterium tuberculosis provides evidence for gene conversion and epitope variation.

    abstract::The 23-membered Esx protein family is involved in the host-pathogen interactions of Mycobacterium tuberculosis. These secreted proteins are among the most immunodominant antigens recognized by the human immune system and have thus been used to develop vaccines and immunodiagnostic tests for tuberculosis (TB). Gene pai...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.05344-11

    authors: Uplekar S,Heym B,Friocourt V,Rougemont J,Cole ST

    更新日期:2011-10-01 00:00:00

  • Cross-sectional analysis of clinical and environmental isolates of Pseudomonas aeruginosa: biofilm formation, virulence, and genome diversity.

    abstract::Chronic lung infections with Pseudomonas aeruginosa biofilms are associated with refractory and fatal pneumonia in cystic fibrosis (CF). In this study, a group of genomically diverse P. aeruginosa isolates were compared with the reference strain PAO1 to assess the roles of motility, twitching, growth rate, and overpro...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.72.1.133-144.2004

    authors: Head NE,Yu H

    更新日期:2004-01-01 00:00:00

  • Gamma interferon induces Fas-dependent apoptosis of Peyer's patch T cells in mice following peroral infection with Toxoplasma gondii.

    abstract::Since we previously observed a remarkable decrease in the numbers of T cells in the Peyer's patches of the small intestines in C57BL/6 mice following peroral infection with Toxoplasma gondii, we performed studies to examine the mechanism(s) whereby this decrease in numbers of the T cells occurs. We found that apoptoti...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.65.11.4682-4689.1997

    authors: Liesenfeld O,Kosek JC,Suzuki Y

    更新日期:1997-11-01 00:00:00

  • Macrophage Migration Inhibition Studies with Cells from Mice Vaccinated with Cell Walls of Mycobacterium bovis BCG: Characterization of the Experimental System.

    abstract::The macrophage migration inhibition test was applied to the study of delayed hypersensitivity in mice vaccinated intravenously with oil-treated cell walls of Mycobacterium bovis BCG. Migration inhibition of peritoneal exudate cells from sensitized mice was demonstrated directly upon incubation of the cells with purifi...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.1.6.587-594.1970

    authors: Yamamoto K,Anacker RL

    更新日期:1970-06-01 00:00:00

  • Macrophage activation by cord factor (trehalose 6,6'-dimycolate): enhanced association with and intracellular killing of Trypanosoma cruzi.

    abstract::Cord factor (trehalose 6,6'-dimycolate[TDM] ), a mixture of 6,6'-diesters of alpha, alpha-D-trehalose with natural mycolic acids, has been described as having immunoregulatory and antitumor activities in vivo, although the relevant mechanisms of action remain unelucidated. In this work, we measured the effects of TDM ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.43.2.531-535.1984

    authors: Kierszenbaum F,Zenian A,Wirth JJ

    更新日期:1984-02-01 00:00:00

  • Experimental elimination of tumor necrosis factor in low-dose endotoxin models has variable effects on survival.

    abstract::Tumor necrosis factor alpha (TNF) is thought to play a major role in the pathogenesis of septic shock. Anti-TNF antibody was preadministered in low-dose endotoxin lethality models in which BALB/c mice were challenged with small amounts of lipopolysaccharide following their sensitization with either carrageenan (CAR) o...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.59.8.2609-2614.1991

    authors: Franks AK,Kujawa KI,Yaffe LJ

    更新日期:1991-08-01 00:00:00

  • Outer membrane protein expression profile in Helicobacter pylori clinical isolates.

    abstract::The gram-negative gastric pathogen Helicobacter pylori is equipped with an extraordinarily large set of outer membrane proteins (OMPs), whose role in the infection process is not well understood. The Hop (Helicobacter outer membrane porins) and Hor (Hop-related proteins) groups constitute a large paralogous family con...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00364-09

    authors: Odenbreit S,Swoboda K,Barwig I,Ruhl S,Borén T,Koletzko S,Haas R

    更新日期:2009-09-01 00:00:00

  • Delineation of a segment of adsorbed salivary acidic proline-rich proteins which promotes adhesion of Streptococcus gordonii to apatitic surfaces.

    abstract::Cells of several strains of Streptococcus gordonii attached in much higher numbers to experimental pellicles formed from samples of submandibular or parotid saliva on hydroxyapatite (HA) beads than to buffer controls. The nature of the salivary components responsible were investigated by preparing experimental pellicl...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.59.9.2948-2954.1991

    authors: Gibbons RJ,Hay DI,Schlesinger DH

    更新日期:1991-09-01 00:00:00

  • The oligomerization domain of C4-binding protein (C4bp) acts as an adjuvant, and the fusion protein comprised of the 19-kilodalton merozoite surface protein 1 fused with the murine C4bp domain protects mice against malaria.

    abstract::Highly purified protein antigens are usually poor immunogens; in practice, adjuvants are needed to obtain satisfactory immune responses. Plasmodium yoelii 19-kDa merozoite surface protein 1 (MSP1(19)) is a weak antigen, but mice vaccinated with this antigen in strong adjuvants can survive an otherwise lethal parasite ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.01369-07

    authors: Ogun SA,Dumon-Seignovert L,Marchand JB,Holder AA,Hill F

    更新日期:2008-08-01 00:00:00