Abstract:
:It has been proposed that a multivalent malaria vaccine is necessary to mimic the naturally acquired resistance to this disease observed in humans. A major experimental challenge is to identify the optimal components to be used in such a multivalent vaccine. Expression library immunization (ELI) is a method for screening genomes of a pathogen to identify novel combinations of vaccine sequences. Here we describe immune responses associated with, and the protective efficacy of, genomic Plasmodium chabaudi adami DS expression libraries constructed in VR1020 (secretory), monocyte chemotactic protein-3 (chemoattractant), and cytotoxic T lymphocyte antigen 4 (lymph node-targeting) DNA vaccine vectors. With splenocytes from vaccinated mice, specific T-cell responses, as well as gamma interferon and interleukin-4 production, were observed after stimulation with P. chabaudi adami-infected erythrocytes, demonstrating the specificity of genomic library vaccination for two of the three libraries constructed. Sera obtained from mice vaccinated with genomic libraries promoted the opsonization of P. chabaudi adami-infected erythrocytes by murine macrophages in vitro, further demonstrating the induction of malaria-specific immune responses following ELI. Over three vaccine trials using biolistic delivery of the three libraries, protection after lethal challenge with P. chabaudi adami DS ranged from 33 to 50%. These results show that protective epitopes or antigens are expressed within the libraries and that ELI induces responses specific to P. chabaudi adami malaria. This study further demonstrates that ELI is a suitable approach for screening the malaria genome to identify the components of multivalent vaccines.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Rainczuk A,Scorza T,Smooker PM,Spithill TWdoi
10.1128/iai.71.8.4506-4515.2003keywords:
subject
Has Abstractpub_date
2003-08-01 00:00:00pages
4506-15issue
8eissn
0019-9567issn
1098-5522journal_volume
71pub_type
杂志文章abstract::The affinity of uropathogenic Escherichia coli to kidneys and bladders of experimentally infected mice was shown to be determined in part by the adhesive properties of the infecting bacteria. Mice were infected with various pairwise combinations of two homogeneic sets of bacteria: (i) mutants derived from a human pyel...
journal_title:Infection and immunity
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doi:10.1128/IAI.40.1.265-272.1983
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.7.4.532-538.1973
更新日期:1973-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.1.402-408.1983
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2011-03-01 00:00:00
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journal_title:Infection and immunity
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更新日期:2010-06-01 00:00:00
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journal_title:Infection and immunity
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更新日期:2008-08-01 00:00:00
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更新日期:2008-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1990-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2004-03-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.11.6461-6465.2000
更新日期:2000-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.12.4.791-798.1975
更新日期:1975-10-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.5.1985-1989.1998
更新日期:1998-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.44.2.320-325.1984
更新日期:1984-05-01 00:00:00
abstract::Peripheral leukocytes from 16 Thai children with dengue hemorrhagic fever were examined to determine the leukocyte composition on the day of presentation and on convalescent days 15 and 30. Mononuclear cells were isolated each time, and the concentrations of T, B, Fc receptor-bearing, and "null" cells were determined....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-05-01 00:00:00
abstract::Streptococcus agalactiae (group B streptococcus [GBS]) is the leading cause of neonatal pneumonia, sepsis, and meningitis. An in silico genome analysis indicated that GBS strain NEM316 encodes 35 proteins containing an LPXTG motif which are thought to be covalently linked to the peptidoglycan by an enzyme called sorta...
journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2005-06-01 00:00:00
abstract::Streptococcus pneumoniae infection leads to multifold increases in sialyltransferase, galactosyltransferase, alpha 2-fucosyltransferase, and alpha 3-fucosyltransferase activity of rat liver. Such changes may reflect an increased demand for glycosylation of acute-phase proteins synthesized and secreted by the liver dur...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.29.1.114-118.1980
更新日期:1980-07-01 00:00:00
abstract::Interactions between Yersinia enterocolitica and rabbit ileal mucus were examined. Strains carrying the Yersinia virulence plasmid, pYV, adhered to crude mucus but not to intestinal luminal contents that had been immobilized on polystyrene. Using an Y. enterocolitica O:9 mutant in which the yadA gene (formerly called ...
journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1991-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.6.1260-1265.1974
更新日期:1974-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.2.329-334.1987
更新日期:1987-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1997-09-01 00:00:00