Abstract:
:An antigenic complex has been isolated in a highly purified from from the Melvin strain of Neisseria gonorrhoeae. The complex has a molecular weight of 9.3 x 10(6) and on sodium dodecyl sulfate-polyacrylamide gel electrophoresis was found to consist of several subunits; the most predominant had the following molecular weights: 110,000, 94,000, 68,000, a smear containing (52,000, 48,000, and 44,000), 42,000, 36,000, 29,000, 28,000, 26,000, and 12,000 comprising 89% of the total protein. With the exception of the subunit of molecular weight 110,000, no change in the content or the mobility of other subunits was observed when beta-mercaptoethanol was omitted from the denaturation solution of sodium dodecyl sulfate electrophoresis. Amino acid analysis of the complex showed a predominance of hydrophobic amino acids. These data implicated noncovalent interactions between the subunits. When the cells were labeled with fluorescamine it was possible to obtain a fluorescent complex with identical properties. Among several buffers used for the isolation of the complex, 0.2 M tris(hydroxymethyl)aminomethane buffer (pH 7.5) gave maximum yield with low amounts of lipopolysaccharide and phospholipid; the choice of the buffer for column chromatography did not seem to make any difference. The high protein content and low amounts of lipopolysaccharide and phospholipid are characteristic properties of the complex.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Karkhanis YD,Anderson RL,Zeltner JY,Carlo DJ,Stoudt THdoi
10.1128/IAI.25.2.635-644.1979keywords:
subject
Has Abstractpub_date
1979-08-01 00:00:00pages
635-44issue
2eissn
0019-9567issn
1098-5522journal_volume
25pub_type
杂志文章abstract::Salmonella enterica serovar Enteritidis (S. Enteritidis) is a major etiologic agent of nontyphoid salmonellosis in the United States. S. Enteritidis persistently and silently colonizes the intestinal and reproductive tract of laying hens, resulting in contaminated poultry products. The consumption of contaminated poul...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02387-14
更新日期:2014-12-01 00:00:00
abstract::Normal ICR mice were infected intravenously, intraperitoneally, or aerogenically with Pasteurella multocida strains isolated from a turkey (S68), calf (V90), or rabbit (J20) lung. Both the turkey and calf isolates were highly virulent for mice and multiplied logarithmically in the lungs, liver, and spleen, resulting i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.2.779-784.1983
更新日期:1983-02-01 00:00:00
abstract::An immunological assay utilizing double-diffusion principles was developed for identification of Pseudomonas aeruginosa mutants deficient in toxin A. Mutations were chemically induced, and mutants were isolated from P. aeruginosa strain PA103. Quantitative assays, both enzymatic and immunological, indicated that five ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-06-01 00:00:00
abstract::Amastigotes obtained from spleens of mice infected with different strains of Trypanosoma cruzi were examined for their ability to invade macrophages and L929 cells and to initiate infection in mice. Both types of cells were readily invaded by organisms of the strains Y, MR, and Tulahuen. Organisms of the CL strain wer...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.46.2.372-376.1984
更新日期:1984-11-01 00:00:00
abstract::Mycobacterium bovis BCG is widely used as a vaccine against tuberculosis (TB), despite its variable protective efficacy. Relatively little is known about the immune response profiles following BCG vaccination in relation to protection against TB. Here we tested whether BCG vaccination results in immune responses to Do...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01999-06
更新日期:2007-07-01 00:00:00
abstract::Five mutagens were used to induce mutants of Candida albicans. Ultraviolet light, N-nitroso-N'-methyl-N-nitrosoguanidine, and N-nitroso-N-methylurethane were effective mutagens which induced stable auxotrophs. N-nitroso-N-methylurethane produced the largest number and variety of mutants. Nitrous acid and hydroxylamine...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.3.1.141-148.1971
更新日期:1971-01-01 00:00:00
abstract::Mycobacterium tuberculosis has a unique family of PE-PGRS proteins with conserved N-terminal domains (PE) containing site-specific proline-glutamine residues and polymorphic GC-rich repetitive sequences (PGRS). Tuberculosis (TB) patients produce antibodies against some such proteins, but it is not clear whether these ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01175-08
更新日期:2009-08-01 00:00:00
abstract::The effect of a standard regimen of cimetidine on the gastric flora of 20 male volunteers was studied in a double-blind manner and compared with the effects of a standard antacid regimen. Postprandial microbial titers in gastric aspirates were significantly higher at 4, 8, and 16 weeks of therapy in subjects taking an...
journal_title:Infection and immunity
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1128/IAI.36.2.518-524.1982
更新日期:1982-05-01 00:00:00
abstract::Herein we characterized various genetic markers and the biological behavior of a natural recombinant strain of Toxoplasma gondii (P-Br). From nine genetic markers analyzed, three (B1, ROP1, and SAG1) and three (cS10-A6, GRA6, and SAG3) markers belong to parasites from the type I and type III lineages, respectively. Th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.11.6392-6401.2003
更新日期:2003-11-01 00:00:00
abstract::Oligosaccharide (OS)-protein conjugates are promising candidate vaccines against encapsulated bacteria, such as Haemophilus influenzae, Neisseria meningitidis, and Streptococcus pneumoniae. Although the effects of several variables such as OS chain length and protein carrier have been studied, little is known about th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.12.6915-6920.2003
更新日期:2003-12-01 00:00:00
abstract::The Leishmania pifanoi amastigote antigen P-8 has been previously shown to induce protective immunity in a murine model of cutaneous leishmaniasis (L. Soong, S. M. Duboise, P. Kima, and D. McMahon-Pratt, Infect. Immun. 63:3559-3566, 1995). As this antigen is of interest for further vaccine studies, the biochemical cha...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.11.6776-6784.2001
更新日期:2001-11-01 00:00:00
abstract::Capsular types 5 and 8, which account for about 70% of Staphylococcus aureus strains isolated from the blood of patients, resisted in vitro phagocytosis by human polymorphonuclear leukocytes (PMN). Antisera and monoclonal antibody to type 5 and 8 capsular polysaccharides (CPS) induced type-specific in vitro phagocytos...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.5.1090-1095.1988
更新日期:1988-05-01 00:00:00
abstract::Legionella pneumophila and Coxiella burnetii are phylogenetically related intracellular bacteria that cause aerosol-transmitted lung infections. In host cells both pathogens proliferate in vacuoles whose biogenesis displays some common features. To test the functional similarity of their respective intracellular niche...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.8.4494-4504.2005
更新日期:2005-08-01 00:00:00
abstract::Helicobacter pylori infection of the stomach causes an active immune response that includes stimulation of inducible nitric oxide (NO) synthase (iNOS) expression. Although NO can kill H. pylori, the bacterium persists indefinitely, suggesting that NO production is inadequate. We determined if the NO derived from iNOS ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00578-07
更新日期:2007-09-01 00:00:00
abstract::The adherence of Treponema denticola GM-1, TD-4, and MS25 to human gingival fibroblasts (HGFs) was studied to serve as an introduction to investigations into the interactions of these oral bacteria with human host cells. Under both aerobic (5% CO2) and anaerobic (85% N2 plus 10% H2 plus 5% CO2) environments, the inter...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.6.1720-1729.1990
更新日期:1990-06-01 00:00:00
abstract::When spleen cells derived from C57BL/6 mice immunized with L cells 7 days previously were cocultured with antigenic cells, immune interferon appeared in the culture fluid. We analyzed the tissue distribution of the immune interferon-producing cells (IIPC) which appeared in various lymphoid organs after allogeneic stim...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-08-01 00:00:00
abstract::Leptospira interrogans is an important mammalian pathogen. Transmission from an environmental source requires adaptations to a range of new environmental conditions in the organs and tissues of the infected host. Since many pathogenic bacteria utilize temperature to discern their environment and regulate the synthesis...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.1.400-404.2001
更新日期:2001-01-01 00:00:00
abstract::A radioimmunoprecipitation method has been devised which permits the direct identification of those proteins which are both exposed on the cell surface of Haemophilus influenzae type b and accessible to antibody. Both extrinsically and intrinsically radiolabeled cells of this organism were employed as the sources of a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.33.3.950-953.1981
更新日期:1981-09-01 00:00:00
abstract::In the progression of the life cycle of Plasmodium falciparum, a small proportion of asexual parasites differentiate into male or female sexual forms called gametocytes. Just like their asexual counterparts, gametocytes are contained within the infected host's erythrocytes (RBCs). However, unlike their asexual partner...
journal_title:Infection and immunity
pub_type: 杂志文章,评审
doi:10.1128/IAI.00775-18
更新日期:2019-05-21 00:00:00
abstract::Citrobacter rodentium, an attaching-effacing bacterial pathogen, establishes an acute infection of the murine colonic epithelium and induces a mild colitis in immunocompetent mice. This study describes the role of T-cell subsets and B lymphocytes in immunity to C. rodentium. C57Bl/6 mice orally infected with C. rodent...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.9.5077-5086.2003
更新日期:2003-09-01 00:00:00
abstract::Resurgence is a major concern after malaria elimination. After the initiation of the elimination program on Aneityum Island in 1991, microscopy showed that Plasmodium falciparum disappeared immediately, whereas P. vivax disappeared from 1996 onward, until P. vivax cases were reported in January 2002. By conducting mal...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00931-13
更新日期:2014-01-01 00:00:00
abstract::Previous studies have shown that tumor necrosis factor alpha (TNF-alpha) plays a pathophysiologic role in sepsis induced in rat pups by group B streptococci (GBS). In this model, TNF-alpha is also partially responsible for the induction of interleukin-6 (IL-6). The present study was undertaken to investigate the role ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.11.4997-5002.1994
更新日期:1994-11-01 00:00:00
abstract::A 95-kDa protein was isolated from Staphylococcus saprophyticus 7108 grown on dialysis membranes placed on the surface of brain heart infusion agar. Strain CCM883 did not produce this protein. Ultrathin sections revealed the presence of very thin, tuftlike, 50- to 75-nm-long structures on the surface of strain 7108, w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.3.1055-1060.1992
更新日期:1992-03-01 00:00:00
abstract::The nucleotide sequences of three previously undescribed Pseudomonas aeruginosa pilin structural genes are presented. Comparisons of deduced pilin primary structure and flanking DNA sequence allowed placement of these and six previously published sequences into one of two groups. Epitope mapping, using overlapping imm...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.2.371-376.1994
更新日期:1994-02-01 00:00:00
abstract::Lung dendritic cells were identified by immunohistochemistry in lung tissue sections from C57BL/6 mice. Following isolation from the lungs using CD11c magnetic beads, the flow cytometric analysis of I-A(b+) and CD11c(+) cells indicated a mixed population of dendritic cells at different stages of maturation, with most ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.2.1127-1133.2001
更新日期:2001-02-01 00:00:00
abstract::We have previously identified mgrA (rat) as a regulator of autolysis in Staphylococcus aureus. Besides its effect on autolytic activity, we recently found alterations in the expression of regulator and target virulence genes in the mgrA mutant. Northern analysis and transcription fusion assays showed that inactivation...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.3.1423-1431.2005
更新日期:2005-03-01 00:00:00
abstract::We produced anti-idiotype antibodies to antibody to lipid A from Eikenella corrodens. The ALA-1 monoclonal antibody (immunoglobulin M [IgM] isotype), which had already been produced in our laboratory (T. Kato, I. Takazoe, and K. Okuda, Infect. Immun. 57:656-659, 1989), had reacted strongly with lipid A from E. corrode...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.2.416-420.1990
更新日期:1990-02-01 00:00:00
abstract::A multigene family of 58- to 60-kDa proteins, which are designated rhoptry-associated protein 1 (RAP-1) and which come from the parasites Babesia bigemina and Babesia bovis, is a target for vaccine development. The presence of multiple gene copies and conserved sequences and epitopes of RAP-1 implies that these protei...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.6.2079-2087.1996
更新日期:1996-06-01 00:00:00
abstract::Hamster trachea organ cultres were shown to consume measurable amounts of oxygen when incubated in a closed micro-chamber. Several simple and complex media were evaluated, and the optimal system involved incubating 2 to 4 mg of tracheal tissue in Tyrodes balanced salt solution at 37 C for 60 min, with oxygen activity ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.12.3.544-549.1975
更新日期:1975-09-01 00:00:00
abstract::The leukotrienes and tumor necrosis factor (TNF) play an important role in the pathophysiology of septic shock, in which hypotension, leukopenia, thrombocytopenia, and hemoconcentration are observed. This study was performed to examine the effects of a 5-lipoxygenase inhibitor (AA-861), a selective leukotriene recepto...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.6.2432-2437.1992
更新日期:1992-06-01 00:00:00