Abstract:
:Legionella pneumophila and Coxiella burnetii are phylogenetically related intracellular bacteria that cause aerosol-transmitted lung infections. In host cells both pathogens proliferate in vacuoles whose biogenesis displays some common features. To test the functional similarity of their respective intracellular niches, African green monkey kidney epithelial (Vero) cells, A/J mouse bone marrow-derived macrophages, human macrophages, and human dendritic cells (DC) containing mature C. burnetii replication vacuoles were superinfected with L. pneumophila, and then the acidity, lysosome-associated membrane protein (LAMP) content, and cohabitation of mature replication vacuoles was assessed. In all cell types, wild-type L. pneumophila occupied distinct vacuoles in close association with acidic, LAMP-positive C. burnetii replication vacuoles. In murine macrophages, but not primate macrophages, DC, or epithelial cells, L. pneumophila replication vacuoles were acidic and LAMP positive. Unlike wild-type L. pneumophila, type IV secretion-deficient dotA mutants trafficked to lysosome-like C. burnetii vacuoles in Vero cells where they survived but failed to replicate. In primate macrophages, DC, or epithelial cells, growth of L. pneumophila was as robust in superinfected cell cultures as in those singly infected. Thus, despite their noted similarities, L. pneumophila and C. burnetii are exquisitely adapted for replication in unique replication vacuoles, and factors that maintain the C. burnetii replication vacuole do not alter biogenesis of an adjacent L. pneumophila replication vacuole. Moreover, L. pneumophila can replicate efficiently in either lysosomal vacuoles of A/J mouse cells or in nonlysosomal vacuoles of primate cells.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Sauer JD,Shannon JG,Howe D,Hayes SF,Swanson MS,Heinzen RAdoi
10.1128/IAI.73.8.4494-4504.2005keywords:
subject
Has Abstractpub_date
2005-08-01 00:00:00pages
4494-504issue
8eissn
0019-9567issn
1098-5522pii
73/8/4494journal_volume
73pub_type
杂志文章abstract::Acute lethality was induced in rabbits by the sequential injection of Propionibacterium acnes and lipopolysaccharide (LPS). P. acnes induced the infiltration of inflammatory cells into the liver lobules during the early phase, and LPS in the late phase caused death in association with pathological changes mimicking he...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.12.4812-4817.1995
更新日期:1995-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.10.3451-3462.1991
更新日期:1991-10-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.62.2.713-716.1994
更新日期:1994-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.2.488-494.1985
更新日期:1985-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.9.3139-3142.1990
更新日期:1990-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/IAI.66.2.771-776.1998
更新日期:1998-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.6.1489-1495.1990
更新日期:1990-06-01 00:00:00
abstract::Sodium dodecyl sulfate-polyacrylamide gel electrophoresis patterns of proteins and externally exposed labeled surface constituents were analyzed in promastigotes of three etiological agents of kala azar (Leishmania donovani, HS70 strain from India; L. chagasi, Imperatriz strain from Brazil; L. infantum, ITMPA K263 str...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.1.136-141.1985
更新日期:1985-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.7.2241-2244.1990
更新日期:1990-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.4.1408-1412.1998
更新日期:1998-04-01 00:00:00
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更新日期:2004-03-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.01237-10
更新日期:2011-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.12.8407-8410.2005
更新日期:2005-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.9.3343-3345.1991
更新日期:1991-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.3.1588-1596.2006
更新日期:2006-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.2.794-799.2001
更新日期:2001-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.1.141-151.1983
更新日期:1983-10-01 00:00:00
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pub_type: 杂志文章
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journal_title:Infection and immunity
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doi:10.1128/IAI.61.7.2960-2965.1993
更新日期:1993-07-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1992-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.29.1.278-280.1980
更新日期:1980-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.3.976-983.1995
更新日期:1995-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1998-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.2.809-814.2000
更新日期:2000-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2004-11-01 00:00:00
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journal_title:Infection and immunity
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更新日期:2018-04-23 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.1.29-34.1987
更新日期:1987-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.2.439-444.1977
更新日期:1977-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.8.3947-3951.1999
更新日期:1999-08-01 00:00:00
abstract::Yersinia pseudotuberculosis inv mutant strains cured of the virulence plasmid exhibit thermoinducible adhesion to cultured mammalian cells. To identify the genes responsible for this phenotype, Y. pseudotuberculosis homologs of the Y. enterocolitica ail and the Y. pestis psa loci were identified. Mutations in the Y. p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.7.2483-2489.1996
更新日期:1996-07-01 00:00:00