Abstract:
:Fifty Borellia burgdorferi strains isolated from humans and ticks in Europe and the United States were analyzed by multilocus enzyme electrophoresis. Eleven genetic loci were characterized on the basis of the electrophoretic mobilities of their products. Ten loci were polymorphic. The average number of alleles per locus was 5.9, with a mean genetic diversity of 0.673 among electrophoretic types (ETs). The strains were grouped into 35 ETs constituting three main divisions (I, II, and III) separated at a genetic distance greater than 0.75. Divisions I, II, and III contained 13, 6, and 16 ETs, respectively. These findings, together with previous data from DNA hybridization and restriction enzyme analysis of rRNA genes, suggest that divisions I, II, and III may represent three distinct genomic species. All three divisions contained human clinical ETs. However, in division I, which includes the ET of the type strain of B. burgdorferi, the human pathogenic ETs constituted a single clone. The ETs of division I were from west-central Europe and the United States, whereas divisions II and III contained ETs from west-central and northern Europe but not from the United States. Finally, our data show that the genetic structure of B. burgdorferi populations is clonal.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Boerlin P,Peter O,Bretz AG,Postic D,Baranton G,Piffaretti JCdoi
10.1128/IAI.60.4.1677-1683.1992keywords:
subject
Has Abstractpub_date
1992-04-01 00:00:00pages
1677-83issue
4eissn
0019-9567issn
1098-5522journal_volume
60pub_type
杂志文章abstract::The immunopathology of Chagas' disease was studied in the experimental model of chronic infection in C57BL/10JT or mice. Sublethal infection with Trypanosoma cruzi, Y strain, induced specific antibodies and a delayed hypersensitivity response to parasite antigens. Mice developed chronic chagasic myocarditis but not sk...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.11.3588-3593.1990
更新日期:1990-11-01 00:00:00
abstract::Serum from rabbits immunized with either heat-killed or live nonencapsulated Staphylococcus aureus failed further to enhance phagocytosis and intracellular killing of the homologous organism by either normal rabbit polymorphonuclear leukocytes or monocytes, when compared with normal rabbit serum. These immune sera did...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.2.6.742-749.1970
更新日期:1970-12-01 00:00:00
abstract::Mouse spleen cultures were infected with murine cytomegalovirus, either before or after separating the cells into various classes, and the growth of virus and production of infectious centers were measured in each cell class. The separation techniques, which were used in various combinations, comprised: adherence to p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.3.853-860.1979
更新日期:1979-12-01 00:00:00
abstract::Memory CD8+ T cells are essential for protective immunity against many intracellular pathogens; therefore, stimulation of this population of cells is an important goal of vaccination. We have previously shown that a detoxified derivative of Bacillus anthracis anthrax lethal toxin (LT) can deliver heterologous CD8+ T-c...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01208-07
更新日期:2008-03-01 00:00:00
abstract::Actinobacillus pleuropneumoniae can use porcine transferrin as the sole source of iron. Two proteins with molecular masses of approximately 60 kDa (TfbA) and 110 kDa have been shown to specifically bind porcine transferrin; from the TfbA protein, three isoforms from A. pleuropneumoniae serotypes 1, 5, and 7 have been ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.10.3846-3850.1995
更新日期:1995-10-01 00:00:00
abstract::Monoclonal antibodies (MAbs) were raised against native and denatured alpha-hemolysin (HlyA) of Escherichia coli. Binding of the MAbs to native, denatured, and erythrocyte-complexed active wild-type hemolysin and mutant derivatives was tested. All 15 MAbs analyzed bound to native hemolysin, even when the toxin was com...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.5.1846-1852.1991
更新日期:1991-05-01 00:00:00
abstract::The effect of a standard regimen of cimetidine on the gastric flora of 20 male volunteers was studied in a double-blind manner and compared with the effects of a standard antacid regimen. Postprandial microbial titers in gastric aspirates were significantly higher at 4, 8, and 16 weeks of therapy in subjects taking an...
journal_title:Infection and immunity
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1128/IAI.36.2.518-524.1982
更新日期:1982-05-01 00:00:00
abstract::Two inbred mouse strains, derived from feral founders, are susceptible to experimental leishmaniasis due to Leishmania major and support a disease of a severity intermediate between those observed in strains C57BL/6 and BALB/c. Mice of the MAI strain develop a severe, nonhealing, but nonfatal disease with no resistanc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.8.4603-4611.2004
更新日期:2004-08-01 00:00:00
abstract::Oligosaccharides (OSs) related to the pneumococcal type 14 capsular polysaccharide (Pn14PS) were studied for their ability to inhibit the binding between anti-PS14 antisera and native PS14. A synthetic tetrasaccharide corresponding to the repeating unit of the Pn14PS, a hexasaccharide mimic, and an octasaccharide frag...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.9.5107-5114.2002
更新日期:2002-09-01 00:00:00
abstract::Mice were inoculated with Legionella pneumophila via an intratracheal route to establish an experimental model of infection. Lung lavage fluid obtained from infected mice contained a cytolytic factor identified as tumor necrosis factor (TNF). Peak levels of TNF were produced at about 24 h postinfection and rapidly dec...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.2.433-437.1987
更新日期:1987-02-01 00:00:00
abstract::In mammalian hosts, Leishmania sp. parasites are obligatory intracellular organisms that invade macrophages and dendritic cells (DC), where they reside in endocytic organelles termed parasitophorous vacuoles (PV). Most of the present knowledge of the characteristics of PV harboring Leishmania sp. is derived from studi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.2.1305-1312.2006
更新日期:2006-02-01 00:00:00
abstract::A battery of lymphoid and nonlymphoid cells was examined for susceptibility to lysis by natural killer cells of chickens. Several susceptible targets were recognized, and most susceptible among these were cells of line LSCC-RP9, derived from a lymphoid tumor induced by Rous-associated virus 2. The natural killer react...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.3.1078-1085.1981
更新日期:1981-03-01 00:00:00
abstract::Bovine septicemic Escherichia coli 31A agglutinates bovine, rabbit, and human erythrocytes and adheres in vitro to the brush border of bovine or ovine intestinal epithelial cells and to the human colon carcinoma Caco-2 cell line. The adhesion and hemagglutination of E. coli 31A are mediated by a chromosome-encoded fim...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.1.332-342.1996
更新日期:1996-01-01 00:00:00
abstract::A hybridoma produced by the polyethylene glycol fusion of the NS-1 variant of the P3x63Ag8 BALB/c plasmacytoma to splenocytes harvested from a BALB/c mouse immunized with whole gonococci was found to be producing antibody to a common region on gonococcal lipopolysaccharide (LPS). Enzyme-linked immunosorbent assay inhi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.3.751-756.1981
更新日期:1981-12-01 00:00:00
abstract::Actinlike material was obtained from disrupted Pseudomonas aeruginosa cells by a modification of the method of Hancock and Nikaido (J. Bacteriol. 136:381-390) for isolating outer membrane vesicles. Pelleted membranes were dissolved in Laemmli sample buffer and electrophoresed in parallel lanes with purified rabbit ske...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.2.483-487.1985
更新日期:1985-11-01 00:00:00
abstract::The enterotoxin of Shigella dysenteriae I produces fluid and electrolyte secretion in the rabbit ileum. These present studies were designed to evaluate nonelectrolyte transport in rabbit ileal mucosa exposed to Shigella enterotoxin. Both 10 mM galactose and 5 mM L-alanine absorptions were significantly impaired in ent...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.2.510-512.1977
更新日期:1977-05-01 00:00:00
abstract::Strains of Bacteroides fragilis associated with diarrhea in children (termed enterotoxigenic B. fragilis, or ETBF) produce a heat-labile ca. 20-kDa protein toxin (BFT). The purpose of this study was to examine the activity of BFT on polarized monolayers of human intestinal epithelial cells (T84 cells). In Ussing chamb...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.9.3561-3570.1997
更新日期:1997-09-01 00:00:00
abstract::The ability of human clinical isolates of Yersinia enterocolitica to interact with HEp-2 cells was found to vary considerably between the serotypes O:3, O:8, and O:9. Although all three serotypes adhered initially to the cell surface, regardless of incubation temperature of the bacterial inoculum or presence of the 40...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.52.1.85-89.1986
更新日期:1986-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00439-09
更新日期:2009-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02001-14
更新日期:2014-12-01 00:00:00
abstract::Subchronic exposure to pharmacological levels of estrogenic compounds, including 17 beta-estradiol, diethylstilbestrol, and alpha-dienestrol, significantly increased the mortality of B6C3F1 female mice after Listeria infection. Compounds with little estrogenic activity, including 5 alpha-dihydrotestosterone, progester...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.46.2.301-307.1984
更新日期:1984-11-01 00:00:00
abstract::The salmonid macrophage-like cell line RTS-11 and purified trout pronephros phagocytes were used to analyze in vitro entry and survival of two Streptococcus iniae serotypes. Efficient invasion by S. iniae occurred in both cells, but only the type II strain persisted in pronephros phagocytes for at least 48 h. Ex vivo ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.5.2318-2325.2003
更新日期:2003-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.3.961-967.1994
更新日期:1994-03-01 00:00:00
abstract::Most vaccines are still given parenterally. Mucosal vaccination would offer different advantages over parenteral immunization, including blocking of the pathogens at the portal of entry. In this paper, nontoxic Escherichia coli heat-labile enterotoxin (LT) mutants and Supramolecular Biovector systems (SMBV) were evalu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.9.4785-4790.2002
更新日期:2002-09-01 00:00:00
abstract::The adherence of Enterococcus faecalis strains to human T24 cells was examined by scanning electron microscopy. Five highly adhesive strains were identified from 30 strains isolated from the urine of patients with urinary tract infections. No efficiently adhesive strains were found among the 30 strains isolated from t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1999-04-01 00:00:00
abstract::The distinctive properties of Brucella outer membrane have been considered to be critical for Brucella sp. virulence. Among the outer membrane molecules possibly related to these properties, Omp10 and Omp19 are immunoreactive outer membrane lipoproteins. Moreover, these proteins of Brucella could constitute a new fami...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.10.5540-5546.2002
更新日期:2002-10-01 00:00:00
abstract::Rinderpest virus infection was shown to induce marked suppression of both humoral antibody response and cell-mediated immunity in rabbits. The virus exhibited a suppressive effect on primary antibody response as indicated by a decrease in numbers of plaque-forming cells (immunoglobulin [Ig]M) and hemagglutinating anti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.2.206-211.1974
更新日期:1974-02-01 00:00:00
abstract::The ability of butyric acid, an extracellular metabolite from periodontopathic bacteria, to induce apoptosis in murine WEHI 231 cells, splenic B cells, and human RAJI cells was examined. The culture filtrate of Porphyromonas gingivalis, Prevotella loescheii, and Fusobacterium nucleatum, which contains high a percentag...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.6.2587-2594.1998
更新日期:1998-06-01 00:00:00
abstract::The mechanism by which Helicobacter pylori, which has little or no invasive activity, induces gastric-tissue inflammation and injury has not been well characterized. We have previously demonstrated that water-extracted proteins of H. pylori are capable of activating human monocytes by a lipopolysaccharide (LPS)-indepe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.4.1183-1187.1995
更新日期:1995-04-01 00:00:00
abstract::The cytotoxic effects of bacterial lipopolysaccharides (LPS) on mouse leukocytes have been examined in vivo and in vitro. Intraperitoneal injection of LPS into C57BL/6 mice greatly reduced the recovery of mononuclear cells; LPS was cytotoxic for macrophages, but had a mitogenic effect on lymphocytes. Similar effects o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.1.310-319.1978
更新日期:1978-07-01 00:00:00