Population genetic analysis of Borrelia burgdorferi isolates by multilocus enzyme electrophoresis.

Abstract:

:Fifty Borellia burgdorferi strains isolated from humans and ticks in Europe and the United States were analyzed by multilocus enzyme electrophoresis. Eleven genetic loci were characterized on the basis of the electrophoretic mobilities of their products. Ten loci were polymorphic. The average number of alleles per locus was 5.9, with a mean genetic diversity of 0.673 among electrophoretic types (ETs). The strains were grouped into 35 ETs constituting three main divisions (I, II, and III) separated at a genetic distance greater than 0.75. Divisions I, II, and III contained 13, 6, and 16 ETs, respectively. These findings, together with previous data from DNA hybridization and restriction enzyme analysis of rRNA genes, suggest that divisions I, II, and III may represent three distinct genomic species. All three divisions contained human clinical ETs. However, in division I, which includes the ET of the type strain of B. burgdorferi, the human pathogenic ETs constituted a single clone. The ETs of division I were from west-central Europe and the United States, whereas divisions II and III contained ETs from west-central and northern Europe but not from the United States. Finally, our data show that the genetic structure of B. burgdorferi populations is clonal.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Boerlin P,Peter O,Bretz AG,Postic D,Baranton G,Piffaretti JC

doi

10.1128/IAI.60.4.1677-1683.1992

keywords:

subject

Has Abstract

pub_date

1992-04-01 00:00:00

pages

1677-83

issue

4

eissn

0019-9567

issn

1098-5522

journal_volume

60

pub_type

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