Abstract:
:Oligosaccharides (OSs) related to the pneumococcal type 14 capsular polysaccharide (Pn14PS) were studied for their ability to inhibit the binding between anti-PS14 antisera and native PS14. A synthetic tetrasaccharide corresponding to the repeating unit of the Pn14PS, a hexasaccharide mimic, and an octasaccharide fragment obtained by Pn14PS depolymerization were good inhibitors. CRM197 conjugates of the tetrasaccharide and an octasaccharide mimic were prepared by using either adipic acid diester or diethyl squarate linkers. The conjugate with the tetrasaccharide chains induced anti-Pn14PS antibodies when injected subcutaneously into mice, as determined by an enzyme-linked immunosorbent assay, and antibody titers increased with oligosaccharide loading. The adipic acid-linked tetrasaccharide conjugates elicited higher antibody titers than those prepared with a squarate spacer. The lower anti-Pn14PS antibody response of the octasaccharide mimic conjugate indicates the importance of the backbone galactose residue for an appropriate antibody response. The OS-CRM197 conjugate prepared from a single repeat unit of the Pn14PS is a potential vaccine candidate.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Mawas F,Niggemann J,Jones C,Corbel MJ,Kamerling JP,Vliegenthart JFdoi
10.1128/iai.70.9.5107-5114.2002keywords:
subject
Has Abstractpub_date
2002-09-01 00:00:00pages
5107-14issue
9eissn
0019-9567issn
1098-5522journal_volume
70pub_type
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journal_title:Infection and immunity
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更新日期:2001-10-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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doi:10.1128/IAI.5.3.311-318.1972
更新日期:1972-03-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.64.7.2483-2489.1996
更新日期:1996-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.33.3.950-953.1981
更新日期:1981-09-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:
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pub_type: 杂志文章
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更新日期:2000-07-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1981-04-01 00:00:00