Transcription of clumping factor A in attached and unattached Staphylococcus aureus in vitro and during device-related infection.


:Staphylococcus aureus is one of the pathogens most frequently isolated in device-related infections. S. aureus is equipped with surface-associated proteins promoting specific binding to matrix molecules. Clumping factor A (ClfA, encoded by clfA) mediates adhesion to fibrinogen. Whereas the contribution of ClfA to pathogenicity is well documented, the influence of different growth and host parameters on gene activity is unclear. To elucidate this question, we investigated clfA transcript levels in an animal model of device-related infection and in planktonic and sessile bacteria grown in vitro. Specific mRNA from the S. aureus strains Newman, Reynolds, and RN6390 was quantified by LightCycler reverse transcription-PCR. In vitro, clfA transcript levels were low in the early logarithmic growth phase, but a clear increase was observed after the late logarithmic phase. Quantities of clfA transcripts were four to six times higher in the planktonic than in the sessile bacterial subpopulations grown to the stationary phase. During infection, in strains Newman and Reynolds levels of clfA transcripts in exudates accumulating in the infected devices were lower than those in the bacteria grown in vitro to stationary phase. clfA mRNA levels in the exudates increased during the initial phase of infection and remained constant after 96 h postinoculation. In contrast to the in vitro results, quantities of clfA transcripts in the unattached bacteria of the exudates never exceeded the level of clfA transcripts in the sessile bacteria attached to glass beads. However, a clear increase in clfA quantities in the sessile bacteria was observed late in infection after 144 h. In conclusion, maximal clfA transcript levels are reached late during growth in vitro and in vivo.


Infect Immun


Infection and immunity


Wolz C,Goerke C,Landmann R,Zimmerli W,Fluckiger U





Has Abstract


2002-06-01 00:00:00












  • Intestinal immune responses to an inactivated oral enterotoxigenic Escherichia coli vaccine and associated immunoglobulin A responses in blood.

    abstract::An inactivated oral enterotoxigenic Escherichia coli (ETEC) vaccine against ETEC diarrhea was given to 25 adult Swedish volunteers. The vaccine consisted of formalin-killed E. coli bacteria expressing the most common colonization factor antigens (CFAs), i.e., CFA/I, -II, and -IV, and recombinantly produced cholera B s...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Ahrén C,Jertborn M,Svennerholm AM

    更新日期:1998-07-01 00:00:00

  • Chromosomal determinants for exfoliative toxin production in two strains of staphylococci.

    abstract::Strain TG is a plasmidless exfoliative toxin (ET) producer. Strain ER201 contains a plasmid, whose loss had no apparent effect on ET production. The plasmid of ER201 was almost identical in size to the plasmid from strain UT0007, which carries an ET determinant. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Rosenblum ED,Tyrone S

    更新日期:1976-11-01 00:00:00

  • Induction of capping in blood-stage trypomastigotes of Trypanosoma cruzi by human anti-Trypanosoma cruzi antibodies.

    abstract::The effect of human-specific antibody on surface membrane antigens of trypomastigotes of Trypanosoma cruzi was studied in vitro by using immunofluorescence methods. Immune sera induced aggregation of surface antigens in trypomastigotes to form polar cell caps. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Schmuñis GA,Szarfman A,Langembach T,de Souza W

    更新日期:1978-05-01 00:00:00

  • Virulence of R-Factor-Bearing Salmonella typhimurium.

    abstract::Antibiotic-resistant recombinants obtained from mating antibiotic-resistant Escherichia coli with virulent, antibiotic-sensitive Salmonella typhimurium are generally avirulent. After 32 consecutive transfers, two of four avirulent recombinants regained their virulence without loss of episome-mediated resistance. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Thiele EH

    更新日期:1970-10-01 00:00:00

  • Toxicity of lipopolysaccharide and of soluble extracts of Salmonella typhimurium in mice immunized with a live attenuated aroA salmonella vaccine.

    abstract::Mice immunized intravenously 10 days earlier (but not those immunized 2 months earlier) with an attenuated Salmonella typhimurium SL3261 aroA live vaccine and tested for delayed-type hypersensitivity by injection of crude Salmonella extracts in the footpad can die within 24 to 48 h of an unexplained allergic reaction....

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Mastroeni P,Villarreal-Ramos B,Harrison JA,Demarco de Hormaeche R,Hormaeche CE

    更新日期:1994-06-01 00:00:00

  • A tightly regulated surface protein of Borrelia burgdorferi is not essential to the mouse-tick infectious cycle.

    abstract::Borrelia burgdorferi synthesizes a variety of differentially regulated outer surface lipoproteins in the tick vector and in vertebrate hosts. Among these is OspD, a protein that is highly induced in vitro by conditions that mimic the tick environment. Using genetically engineered strains in which ospD is deleted, we d...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Stewart PE,Bestor A,Cullen JN,Rosa PA

    更新日期:2008-05-01 00:00:00

  • Division of single host cells after infection with chlamydiae.

    abstract::Mouse fibroblasts (L cells) were infected in suspension with Chlamydia psittaci (6BC) and then plated out on a solid substrate at a density of 80 cells per cm2 so that the effect of chlamydial infection on the division of single host cells and their progeny could be determined. Uninfected L cells multiplied with a mea...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Horoschak KD,Moulder JW

    更新日期:1978-01-01 00:00:00

  • Genetic, enzymatic, and pathogenic studies of the iron superoxide dismutase of Campylobacter jejuni.

    abstract::Campylobacter jejuni is a microaerobic bacterium that produces an acute, self-limiting, watery or bloody diarrhea in humans. Little is known about how C. jejuni causes disease or even what specific capabilities it requires for survival in vivo. The enzyme, superoxide dismutase (SOD), which catalyzes the breakdown of s...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Pesci EC,Cottle DL,Pickett CL

    更新日期:1994-07-01 00:00:00

  • Bovine NK cells can produce gamma interferon in response to the secreted mycobacterial proteins ESAT-6 and MPP14 but not in response to MPB70.

    abstract::Bovine NK cells have recently been characterized and the present study describes the interaction between NK cells, antigen-presenting cells, and secreted mycobacterial proteins. Gamma interferon (IFN-gamma) production by NK cells was seen in approximately 30% of noninfected calves in response to the Mycobacterium tube...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Olsen I,Boysen P,Kulberg S,Hope JC,Jungersen G,Storset AK

    更新日期:2005-09-01 00:00:00

  • Localization and developmental regulation of a dispersed gene family 1 protein in Trypanosoma cruzi.

    abstract::The dispersed gene family 1 (DGF-1) is the fifth largest gene family in the Trypanosoma cruzi genome, with over 500 members (11). Many of the predicted DGF-1 protein products have several transmembrane domains and N-glycosylation and phosphorylation sites and were thought to localize in the plasma membrane. Here, we r...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Lander N,Bernal C,Diez N,Añez N,Docampo R,Ramírez JL

    更新日期:2010-01-01 00:00:00

  • The increased accumulation of Staphylococcus aureus virulence factors is maximized in a purR mutant by the increased production of SarA and decreased production of extracellular proteases.

    abstract::Mutation of purR was previously shown to enhance the virulence of Staphylococcus aureus in a murine sepsis model, and this cannot be fully explained by increased expression of genes within the purine biosynthesis pathway. Rather, the increased production of specific S. aureus virulence factors, including alpha toxin a...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Alkam D,Jenjaroenpun P,Ramirez AM,Beenken KE,Spencer HJ,Smeltzer MS

    更新日期:2021-01-19 00:00:00

  • Interactions between endogenous gamma interferon and tumor necrosis factor in host resistance against primary and secondary Listeria monocytogenes infections.

    abstract::Intravenous injection of rat anti-mouse gamma interferon (IFN-gamma) monoclonal antibody as well as rabbit anti-mouse tumor necrosis factor (TNF) antibody into mice which had received a sublethal infection with Listeria monocytogenes cells resulted in acceleration of listeriosis. Endogenous IFN-gamma seemed to be prod...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Nakane A,Minagawa T,Kohanawa M,Chen Y,Sato H,Moriyama M,Tsuruoka N

    更新日期:1989-11-01 00:00:00

  • Lipids of putative relevance to virulence in Mycobacterium tuberculosis: phthiocerol dimycocerosate and the attenuation indicator lipid.

    abstract::Lipid compositions of 40 patient isolates of Mycobacterium tuberculosis derived from Madras, Burma, Rangoon, and East Africa were studied, and two major populations of tubercle bacilli were distinguished. Nearly all of the strains previously designated as attenuated for the guinea pig (D. A. Mitchison) are characteriz...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Goren MB,Brokl O,Schaefer WB

    更新日期:1974-01-01 00:00:00

  • Association of a major protein antigen of Mycoplasma arthritidis with virulence.

    abstract::Mycoplasma arthritidis causes acute polyarthritis in rats and chronic proliferative arthritis in mice. M. arthritidis-induced arthritis serves as a model for arthritis caused by infectious agents and as a model for examining the role of the superantigen MAM (M. arthritidis T-cell mitogen) in the development of autoimm...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Tu AH,Clapper B,Schoeb TR,Elgavish A,Zhang J,Liu L,Yu H,Dybvig K

    更新日期:2005-01-01 00:00:00

  • Identification and characterization of an autolysin-encoding gene of Streptococcus mutans.

    abstract::We identified a gene (atlA) encoding autolytic activity from Streptococcus mutans Xc. The AtlA protein predicted to be encoded by atlA is composed of 979 amino acids with a molecular weight of 107,279 and has a conserved beta-1,4-N-acetylmuramidase (lysozyme) domain in the C-terminal portion. Sodium dodecyl sulfate ex...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Shibata Y,Kawada M,Nakano Y,Toyoshima K,Yamashita Y

    更新日期:2005-06-01 00:00:00

  • Human response to Escherichia coli O157:H7 infection: antibodies to secreted virulence factors.

    abstract::Vaccination has been proposed for the prevention of disease due to enterohemorrhagic Escherichia coli (EHEC), but the immune response following human infection, including the choice of potential antigens, has not been well characterized. To study this, sera were obtained from five pediatric patients with acute diarrhe...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Li Y,Frey E,Mackenzie AM,Finlay BB

    更新日期:2000-09-01 00:00:00

  • Pseudomonas aeruginosa clearance in mice: comparison of tissue, strain, and corticosteroid effects.

    abstract::We used a murine model to determine whether chronic corticosteroid therapy has uniform effects on bacterial clearance processes in different tissues. After a 2-week regimen of oral prednisolone, Swiss Webster mice were challenged with Pseudomonas aeruginosa strain PAO1 or virulent derivatives of PAO1 (IP-8 and IT-10)....

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Nugent KM,Cox CD,Pesanti EL

    更新日期:1984-03-01 00:00:00

  • Infection-immunity in tularemia: specificity of cellular immunity.

    abstract::The relationship between hypersensitivity and cellular resistance to infection with facultative intracellular parasites was studied in mice by using infection-immunity in tularemia as a model system. Delayed hypersensitivity to antigenic fractions of Francisella tularensis was first detected 6 to 7 days after immuniza...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Claflin JL,Larson CL

    更新日期:1972-03-01 00:00:00

  • Characterization of VspB of Borrelia turicatae, a major outer membrane protein expressed in blood and tissues of mice.

    abstract::Serotypes A and B of the relapsing fever spirochete Borrelia turicatae produce different disease manifestations in infected mice. Whereas serotype B causes more severe arthritis and reaches higher densities in the blood of mice than serotype A, serotype A invades the central nervous system earlier than serotype B duri...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Pennington PM,Cadavid D,Barbour AG

    更新日期:1999-09-01 00:00:00

  • Complement receptor 1 expression on mouse erythrocytes mediates clearance of Streptococcus pneumoniae by immune adherence.

    abstract::Complement-containing immune complexes can be presented to phagocytes by human erythrocytes bearing complement receptor 1 (CR1). Although this has long been assumed to be a mechanism by which humans are able to protect themselves from "extracellular" bacteria such as pneumococci, there is little direct evidence. In th...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Li J,Wang JP,Ghiran I,Cerny A,Szalai AJ,Briles DE,Finberg RW

    更新日期:2010-07-01 00:00:00

  • Comparison of techniques for primary isolation of respiratory Mycoplasma pulmonis from rats.

    abstract::Studies were performed to determine the optimal sites for primary isolation of Mycoplasma pulmonis from the respiratory system of laboratory rats. In a preliminary study, a group of 42 rats was cultured for respiratory M. pulmonis at several sites. Tracheal washes yielded the highest number of positive cultures (61.9%...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Lentsch RH,Wagner JE,Owens DR

    更新日期:1979-11-01 00:00:00

  • CpG oligodeoxynucleotide and Montanide ISA 51 adjuvant combination enhanced the protective efficacy of a subunit malaria vaccine.

    abstract::Unmethylated CpG dinucleotide motifs present in bacterial genomes or synthetic oligodeoxynucleotides (ODNs) serve as strong immunostimulatory agents in mice, monkeys and humans. We determined the adjuvant effect of murine CpG ODN 1826 on the immunogenicity and protective efficacy of the Saccharomyces cerevisiae-expres...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Kumar S,Jones TR,Oakley MS,Zheng H,Kuppusamy SP,Taye A,Krieg AM,Stowers AW,Kaslow DC,Hoffman SL

    更新日期:2004-02-01 00:00:00

  • Haemophilus influenzae resides and multiplies intracellularly in human adenoid tissue as demonstrated by in situ hybridization and bacterial viability assay.

    abstract::The DNA oligomer 5'-d(TGCGGCCTCTCAGTCCCGCACTTTCATCTTCC)-3' specifically recognizes Haemophilus influenzae 16S rRNA. We report here the use of this oligonucleotide, with a fluorescein label tagged on its 5' end, as a probe for the in situ detection of nonencapsulated nontypeable H. influenzae in sections of adenoid tis...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Forsgren J,Samuelson A,Ahlin A,Jonasson J,Rynnel-Dagöö B,Lindberg A

    更新日期:1994-02-01 00:00:00

  • Immune responses to Cryptosporidium muris and Cryptosporidium parvum in adult immunocompetent or immunocompromised (nude and SCID) mice.

    abstract::Adult murine models of Cryptosporidium infection involving Cryptosporidium muris and C. parvum were used to study immunity to cryptosporidiosis in the mammalian host. Immunocompetent BALB/c or C57BL/6 mice developed a highly patent infection with the RN 66 strain of C. muris but overcame the infection and were immune ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: McDonald V,Deer R,Uni S,Iseki M,Bancroft GJ

    更新日期:1992-08-01 00:00:00

  • An Iron-Regulated Autolysin Remodels the Cell Wall To Facilitate Heme Acquisition in Staphylococcus lugdunensis.

    abstract::Bacteria alter their cell surface in response to changing environments, including those encountered upon invasion of a host during infection. One alteration that occurs in several Gram-positive pathogens is the presentation of cell wall-anchored components of the iron-regulated surface determinant (Isd) system, which ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Farrand AJ,Haley KP,Lareau NM,Heilbronner S,McLean JA,Foster T,Skaar EP

    更新日期:2015-09-01 00:00:00

  • Antibody Response to Bacterial Antigens: Characteristics of Antibody Response to Somatic Antigens of Salmonella typhimurium.

    abstract::The character of the antibody response in the rabbit to Salmonella typhimurium somatic (O) antigen was similar to the response to each of several serotypes of Shigella flexneri O antigens, namely a predominance of production of immunoglobulin M (IgM) antibody. Lipopolysaccharide protein (LPSP) and lipopolysaccharide (...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Fukazawa Y,Shinoda T,Yomoda T,Tsuchiya T

    更新日期:1970-03-01 00:00:00

  • Inbred strains derived from feral mice reveal new pathogenic mechanisms of experimental leishmaniasis due to Leishmania major.

    abstract::Two inbred mouse strains, derived from feral founders, are susceptible to experimental leishmaniasis due to Leishmania major and support a disease of a severity intermediate between those observed in strains C57BL/6 and BALB/c. Mice of the MAI strain develop a severe, nonhealing, but nonfatal disease with no resistanc...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Babay BE,Louzir H,Kebaïer C,Boubaker S,Dellagi K,Cazenave PA

    更新日期:2004-08-01 00:00:00

  • Effect of acetylation on arthropathic activity of group A streptococcal peptidoglycan-polysaccharide fragments.

    abstract::Purified group A streptococcal peptidoglycan-polysaccharide (PG-PS) fragments were either de-O-acylated, or acetylated and then de-O-acylated to yield N-acetylated PG-PS. Native PG-PS was poorly degraded, N-acetylated PG-PS was extensively degraded, and de-O-acylated PG-PS was only slightly degraded by hen egg white l...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Stimpson SA,Lerch RA,Cleland DR,Yarnall DP,Clark RL,Cromartie WJ,Schwab JH

    更新日期:1987-01-01 00:00:00

  • Crossed immunoelectrophoretic analysis of anti-Salmonella typhi antibodies in sera of typhoid patients and carriers: demonstration of the presence of typhoid-specific antibodies to a non-O, non-H, non-Vi antigen.

    abstract::A veronal buffer extract of Salmonella typhi was used as the reference antigen and its corresponding rabbit antiserum as the reference antibody in crossed immunoelectrophoresis to analyze antibodies in sera obtained from typhoid patients and carriers. Four precipitating antibodies were regularly detected. Three were a...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Chau PY,Wan KC,Tsang RS

    更新日期:1984-03-01 00:00:00

  • The HMW1 and HMW2 Adhesins Enhance the Ability of Nontypeable Haemophilus influenzae To Colonize the Upper Respiratory Tract of Rhesus Macaques.

    abstract::Nontypeable Haemophilus influenzae (NTHi) initiates infection by colonizing the upper respiratory tract and is a common cause of localized respiratory tract disease. Previous work has established that the NTHi HMW1 and HMW2 proteins are potent adhesins that mediate efficient in vitro adherence to cultured human respir...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Rempe KA,Porsch EA,Wilson JM,St Geme JW 3rd

    更新日期:2016-09-19 00:00:00