Skin-Specific Unsaturated Fatty Acids Boost the Staphylococcus aureus Innate Immune Response.


:Antimicrobial fatty acids (AFAs) protect the human epidermis against invasion by pathogenic bacteria. In this study, we questioned whether human skin fatty acids (FAs) can be incorporated into the lipid moiety of lipoproteins and whether such incorporation would have an impact on innate immune stimulation in the model organism Staphylococcus aureus USA300 JE2. This organism synthesized only saturated FAs. However, when feeding USA300 with unsaturated FAs present on human skin (C16:1, C18:1, or C18:2), those were taken up, elongated stepwise by two carbon units, and finally found in the bacterial (phospho)lipid fraction. They were also observed in the lipid moiety of lipoproteins. When USA300 JE2 was fed with the unsaturated FAs, the cells and cell lysates showed an increased innate immune activation with various immune cells and peripheral blood mononuclear cells (PBMCs). Immune activation was highest with linoleic acid (C18:2). There are several pieces of evidence that the enhanced immune stimulating effect was due to the incorporation of unsaturated FAs in lipoproteins. First, the enhanced stimulation was dependent on Toll-like receptor 2 (TLR2). Second, an lgt mutant, unable to carry out lipidation of prolipoproteins, was unable to carry out immune stimulation when fed with unsaturated FAs. Third, the supplied FAs did not significantly affect growth, protein release, or expression of the model lipoprotein Lpl1. Although S. aureus is unable to synthesize unsaturated FAs, it incorporates long-chain unsaturated FAs into its lipoproteins, with the effect that the cells are better recognized by the innate immune system. This is an additional mechanism how our skin controls bacterial colonization and infection.


Infect Immun


Infection and immunity


Nguyen MT,Hanzelmann D,Härtner T,Peschel A,Götz F




Has Abstract


2015-10-26 00:00:00














  • Characterization of lectinlike surface components on Capnocytophaga ochracea ATCC 33596 that mediate coaggregation with gram-positive oral bacteria.

    abstract::The interactions between Capnocytophaga ochracea ATCC 33596 and Streptococcus sanguis H1, Actinomyces naeslundii PK984, or Actinomyces israelii PK16 are dependent on specific recognitions between heat-sensitive adhesins on C. ochracea and heat-stable structures (probably carbohydrate-containing receptors) on the surfa...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Weiss EI,London J,Kolenbrander PE,Kagermeier AS,Andersen RN

    更新日期:1987-05-01 00:00:00

  • Chlamydia pneumoniae secretion of a protease-like activity factor for degrading host cell transcription factors required for [correction of factors is required for] major histocompatibility complex antigen expression.

    abstract::Chlamydia pneumoniae is a causative agent for many respiratory infections and has been associated with cardiovascular diseases in humans. The pathogenicity of C. pneumoniae is thought to depend on its ability to cause persistent infection and to evade host defense. Genome sequence analysis indicates that C. pneumoniae...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Fan P,Dong F,Huang Y,Zhong G

    更新日期:2002-01-01 00:00:00

  • Glycoproteins of natural origin with an affinity for hepatitis B surface antigen.

    abstract::Sera from certain animal species contain a substance(s) which binds hepatitis B surface antigen. The hepatitis B binding substance found in animals is not antibody, but appears to be a glycoprotein which reacted with antigen-coated beads and produced a "false positive" test for antibody. This glycoprotein could be sel...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Millman I,McMichael JC

    更新日期:1978-09-01 00:00:00

  • Effects of human serum on bacterial competition with neutrophils for molecular oxygen.

    abstract::A dialyzable factor(s) in human serum is known to stimulate gonococcal oxygen consumption. Its effect on other human pathogens was investigated. A 10% serum solution increased peak O2 consumption for Escherichia coli and Staphylococcus aureus to 157% (P less than 0.05) and 199% (P less than 0.02), respectively, of the...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Britigan BE,Cohen MS

    更新日期:1986-06-01 00:00:00

  • Vibrio cholerae expresses cell surface antigens during intestinal infection which are not expressed during in vitro culture.

    abstract::Vibrio cholerae O1 bacteria harvested directly from ligated or nonligated intestines of rabbits with experimental cholera expressed at least 7 to 8 novel, in vivo-specific cell envelope (env) proteins that were not found on vibrios after in vitro culture in various ordinary liquid media. At the same time, several of t...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Jonson G,Svennerholm AM,Holmgren J

    更新日期:1989-06-01 00:00:00

  • Enzyme-linked immunosorbent assay for quantitating the humoral immune response to the colonization factor antigen of enterotoxigenic Escherichia coli.

    abstract::The enzyme-linked immunosorbent assay technique was used to quantitate, in milligrams per milliliter, anti-colonization factor antigen/I (CFA/I) immunoglobulin G (IgG) in acute- and convalescent-phase sera of individuals who experienced diarrhea associated with CFA/I-positive enterotoxigenic Escherichia coli. Purified...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Clegg S,Evans DG,Evans DJ Jr

    更新日期:1980-02-01 00:00:00

  • Identification and quantitation of capsular antigen in capsulated and noncapsulated strains of Haemophilus influenzae type b by crossed-immunoelectrophoresis.

    abstract::Sonicated preparations of capsulated Haemophilus influenzae type b, two of its spontaneous mutants, one containing patches of capsules (class I variant) and the other noncapsulated (class II variant), and a noncapsulated strain of H. influenzae type d were analyzed by crossed-immunoelectrophoresis using unadsorbed ant...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Buckmire FL

    更新日期:1976-06-01 00:00:00

  • Globotriaosylceramide, Gb3, is an alternative functional receptor for Shiga-like toxin 2e.

    abstract::We reexamined the binding specificity of the Shiga-like toxin variant associated with porcine edema disease, SLT2e, which is reported to be more cytotoxic for Vero cells than for HeLa cells, by using receptor-deficient cells and a liposomal insertion system for purified glycolipids. We found that SLT2e preferentially ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Keusch GT,Jacewicz M,Acheson DW,Donohue-Rolfe A,Kane AV,McCluer RH

    更新日期:1995-03-01 00:00:00

  • Characterization of an iroBCDEN gene cluster on a transmissible plasmid of uropathogenic Escherichia coli: evidence for horizontal transfer of a chromosomal virulence factor.

    abstract::The chromosomal iroBCDEN gene cluster first described for Salmonella enterica is involved in the uptake of catecholate-type siderophore compounds. An orthologous gene cluster has recently been detected in Escherichia coli strains which cause extraintestinal disease. This E. coli iroBCDEN gene cluster has an impact on ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Sorsa LJ,Dufke S,Heesemann J,Schubert S

    更新日期:2003-06-01 00:00:00

  • Safety and immunogenicity of a booster dose of Vibrio cholerae CVD 103-HgR live oral cholera vaccine in Swiss adults.

    abstract::Adult volunteers received a booster dose (4 x 10(8) CFU) of attenuated Vibrio cholerae CVD 103-HgR oral vaccine 15 or 24 months after primary immunization. The immune response was modest, presumably due to rapid clearance of the vaccine strain by a primed immune system. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Cryz SJ Jr,Levine MM,Losonsky G,Kaper JB,Althaus B

    更新日期:1992-09-01 00:00:00

  • Production of Biologically Active Substances by Two Strains of Vibrio cholerae.

    abstract::Strains Inaba 569B and Ogawa B1307 of Vibrio cholerae were grown in shaken Casamino Acids-yeast extract-glucose medium at 30 and 37 C for 4 days. Time courses of production of neuraminidase (NM), mucinase (MC), proteinase (PR), lytic factor (LF), and vascular permeability factor (PF) were compared by examining culture...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Kusama H,Craig JP

    更新日期:1970-01-01 00:00:00

  • Immunochemical differences between oral and nonoral strains of Bacteroides asaccharolyticus.

    abstract::We undertook a morphological and immunochemical comparison of purified outer membrane antigens from oral and nonoral isolates of Bacteroides asaccharolyticus. Electron micrographs of thin sections of whole bacteria revealed a compact, electron-dense capsule external to the outer membrane of oral strains. A loose, web-...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Mansheim BJ,Coleman SE

    更新日期:1980-02-01 00:00:00

  • Contribution of clumping factor B to pathogenesis of experimental endocarditis due to Staphylococcus aureus.

    abstract::Staphylococcus aureus Newman with an insertion mutation in clfB, the gene encoding clumping factor B, only marginally decreased infection rate (P>0.05) in rats with experimental endocarditis. In contrast, clfB complementation on a multicopy plasmid significantly increased infectivity (P<0.05) over the deleted mutants....

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Entenza JM,Foster TJ,Ni Eidhin D,Vaudaux P,Francioli P,Moreillon P

    更新日期:2000-09-01 00:00:00

  • Experimental infection in mice with Treponema hyodysenteriae.

    abstract::Nineteen of 22 female mice (CF1 strain) inoculated intragastrically with Treponema hyodysenteriae developed cecal and colonic lesions consisting of catarrhal inflammation, edema, and occasional hemorrhage. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Joens LA,Glock RD

    更新日期:1979-08-01 00:00:00

  • rgf encodes a novel two-component signal transduction system of Streptococcus agalactiae.

    abstract::The adhesion of gram-positive bacteria to extracellular matrix (ECM) proteins is regarded as an important determinant of pathogenicity. A comparison of the adhesion of Streptococcus agalactiae strain O90R to different ECM proteins showed that the most pronounced binding could be observed for immobilized fibrinogen. To...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Spellerberg B,Rozdzinski E,Martin S,Weber-Heynemann J,Lütticken R

    更新日期:2002-05-01 00:00:00

  • Novel 17-kilodalton Leishmania antigen revealed by immunochemical studies of a purified glycoprotein fraction recognized by murine T lymphocytes.

    abstract::Recently, a glycoprotein fraction, designated gp10/20, purified from Leishmania mexicana amazonensis was shown to induce a cellular immune response mediated by murine L3T4+ T lymphocytes. This fact led us to pursue further the characterization of this fraction. The present study demonstrated that gp10/20 is a degradat...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Rodrigues MM,Xavier MT,Mendonça-Previato L,Barcinski MA

    更新日期:1988-07-01 00:00:00

  • Suppressed induction of proinflammatory cytokines by a unique metabolite produced by Vibrio cholerae O1 El Tor biotype in cultured host cells.

    abstract::Vibrio cholerae O1 has two biotypes, El Tor and Classical, and the latter is now presumed to be extinct in nature. Under carbohydrate-rich growth conditions, El Tor biotype strains produce the neutral fermentation end product 2,3-butanediol (2,3-BD), which prevents accumulation of organic acids from mixed acid ferment...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Bari W,Song YJ,Yoon SS

    更新日期:2011-08-01 00:00:00

  • Effect of respiratory syncytial virus and virus-antibody complexes on the oxidative metabolism of human neutrophils.

    abstract::The effect of respiratory syncytial virus (RSV) or mixtures of RSV and its specific antibody on the oxidative metabolic activity of human polymorphonuclear leukocytes was studied by the technique of luminol-dependent chemiluminescence. Peripheral blood neutrophils obtained from normal healthy donors were used. RSV alo...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Kaul TN,Faden H,Ogra PL

    更新日期:1981-05-01 00:00:00

  • ZapA, a virulence factor in a rat model of Proteus mirabilis-induced acute and chronic prostatitis.

    abstract::Our knowledge of pathogenesis has benefited from a better understanding of the roles of specific virulence factors in disease. To determine the role of the virulence factor ZapA, a 54-kDa metalloproteinase of Proteus mirabilis, in prostatitis, rats were infected with either wild-type (WT) P. mirabilis or its isogenic ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Phan V,Belas R,Gilmore BF,Ceri H

    更新日期:2008-11-01 00:00:00

  • Isolation, propagation, and characterization of a newly recognized pathogen, cilia-associated respiratory bacillus of rats, an etiological agent of chronic respiratory disease.

    abstract::A Gram-negative, filamentous, rod-shaped bacillus which failed to grow in cell-free media was isolated in apparently pure culture from the bronchial scraping and washing of a laboratory rat suffering from chronic respiratory disease by inoculating embryonated chicken eggs via the allantoic route. None of the embryos d...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Ganaway JR,Spencer TH,Moore TD,Allen AM

    更新日期:1985-02-01 00:00:00

  • Conformational nature of the Borrelia burgdorferi B31 outer surface protein C protective epitope.

    abstract::Active immunization with Escherichia coli-expressed recombinant outer surface protein C (OspC) of Borrelia burgdorferi has been demonstrated to confer protection against a tick-transmitted infection on laboratory animals. A previous study in this laboratory showed that OspC antibody raised against a denatured immunoge...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Gilmore RD Jr,Mbow ML

    更新日期:1999-10-01 00:00:00

  • Inactivation of suppressor T-cell activity by nontoxic monophosphoryl lipid A.

    abstract::Treatment with nontoxic monophosphoryl lipid A (MPL), which was derived from a polysaccharide-deficient, heptoseless Re mutant of Salmonella typhimurium, was found to inactivate suppressor T-cell activity, as evidenced by a decrease in the degree of low-dose immunological paralysis expressed and an increase in the mag...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Baker PJ,Hiernaux JR,Fauntleroy MB,Prescott B,Cantrell JL,Rudbach JA

    更新日期:1988-05-01 00:00:00

  • Species specificity and lack of production of STb enterotoxin by Escherichia coli strains isolated from humans with diarrheal illness.

    abstract::Escherichia coli strains produce at least two heat-stable enterotoxins, STa and STb. STa is well known to be important in the pathogenesis of human diarrheal disease; the role of STb has not been defined. Fifty-two E. coli strains recovered from human diarrheal illness in northeast Brazil or Bangladesh were examined i...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Weikel CS,Tiemens KM,Moseley SL,Huq IM,Guerrant RL

    更新日期:1986-04-01 00:00:00

  • Protective effect of human granulocyte colony-stimulating factor on microbial infection in neutropenic mice.

    abstract::A purified human granulocyte colony-stimulating factor (hG-CSF) was studied for its protective effect on the induction of neutropenia and enhanced susceptibility to microbial infections in mice receiving cyclophosphamide (CPA). A severe reduction in peripheral blood neutrophils was induced 4 days after injection with ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Matsumoto M,Matsubara S,Matsuno T,Tamura M,Hattori K,Nomura H,Ono M,Yokota T

    更新日期:1987-11-01 00:00:00

  • Leishmania Infection Induces Macrophage Vascular Endothelial Growth Factor A Production in an ARNT/HIF-Dependent Manner.

    abstract::Cutaneous leishmaniasis is characterized by vascular remodeling. Following infection with Leishmania parasites, the vascular endothelial growth factor A (VEGF-A)/VEGF receptor 2 (VEGFR-2) signaling pathway mediates lymphangiogenesis, which is critical for lesion resolution. Therefore, we investigated the cellular and ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Weinkopff T,Roys H,Bowlin A,Scott P

    更新日期:2019-10-18 00:00:00

  • Immunoglobulin subclass distribution and dynamics of Shigella-specific antibody responses in serum and stool samples in shigellosis.

    abstract::To assess the humoral immunological responses at the subclass level in shigellosis, specific antibody responses against Shigella dysenteriae 1 lipopolysaccharide (LPS), S. flexneri Y LPS, invasion plasmid-coded protein antigens (Ipa), and Shiga toxin were analyzed. Antibody responses of 41 patients with S. dysenteriae...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Islam D,Wretlind B,Ryd M,Lindberg AA,Christensson B

    更新日期:1995-05-01 00:00:00

  • Effect of indomethacin on intestinal water transport in salmonella-infected rhesus monkeys.

    abstract::Indomethacin, a nonsteroidal anti-inflammatory agent, will abolish salmonella-induced rabbit ileal secretion when given prior to the establishment of infection. In the present study, we assessed whether indomethacin can inhibit salmonella-induced intestinal secretion when administered after infection and net intestina...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Giannella RA,Rout WR,Formal SB

    更新日期:1977-07-01 00:00:00

  • A conserved PapB family member, TosR, regulates expression of the uropathogenic Escherichia coli RTX nonfimbrial adhesin TosA while conserved LuxR family members TosE and TosF suppress motility.

    abstract::A heterogeneous subset of extraintestinal pathogenic Escherichia coli (ExPEC) strains, referred to as uropathogenic E. coli (UPEC), causes most uncomplicated urinary tract infections. However, no core set of virulence factors exists among UPEC strains. Instead, the focus of the analysis of urovirulence has shifted to ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Engstrom MD,Alteri CJ,Mobley HL

    更新日期:2014-09-01 00:00:00

  • Heterogeneic autoantibody against neurofilament protein in the sera of animals with experimental kuru and Creutzfeldt-Jakob disease and natural scrapie infection.

    abstract::Heterogeneic autoantibodies against axonal neurofilament proteins of mature mouse neurons grown in vitro were detected by the indirect immunofluorescence technique in 12.7% (9 of 71) of the sera from nonhuman primates infected with kuru, in 14.5% (17 of 117) and 4% (1 of 25), respectively, of the sera from nonhuman pr...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Aoki T,Gibbs CJ Jr,Sotelo J,Gajdusek DC

    更新日期:1982-10-01 00:00:00

  • A hemagglutinin of uropathogenic Escherichia coli recognizes the Dr blood group antigen.

    abstract::A receptor moiety and blood group substance recognized by the O75X adhesin was studied. Well-defined erythrocytes representing different blood group systems and bacterial derivatives carrying plasmid pBJN406 encoding the adhesin were used in a direct hemagglutination assay. We showed that Dr blood group antigen, a com...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Nowicki B,Moulds J,Hull R,Hull S

    更新日期:1988-05-01 00:00:00