Abstract:
:The ability of oral immunization to interfere with the establishment of infection with Helicobacter felis was examined. Groups of Swiss Webster mice were immunized orally with 250 micrograms of Helicobacter pylori recombinant urease (rUrease) and 10 micrograms of cholera toxin (CT) adjuvant, 1 mg of H. felis sonicate antigens and CT, or phosphate-buffered saline (PBS) and CT. Oral immunization with rUrease resulted in markedly elevated serum immunoglobulin G (IgG), serum IgA, and intestinal IgA antibody responses. Challenge with live H. felis further stimulated the urease-specific intestinal IgA and serum IgG and IgA antibody levels in mice previously immunized with rUrease but activated primarily the serum IgG compartment of PBS-treated and H. felis-immunized mice. Intestinal IgA and serum IgG and IgA anti-urease antibody responses were highest in rUrease-immunized mice at the termination of the experiment. Mice immunized with rUrease were significantly protected (P < or = 0.0476) against infection when challenged with H. felis 2 or 6 weeks post-oral immunization in comparison with PBS-treated mice. Whereas H. felis-infected mice displayed multifocal gastric mucosal lymphoid follicles consisting of CD45R+ B cells surrounded by clusters of Thy1.2+ T cells, gastric tissue from rUrease-immunized mice contained few CD45R+ B cells and infrequent mucosal follicles. These observations show that oral immunization with rUrease confers protection against H. felis infection and suggest that gastric tissue may function as an effector organ of the mucosal immune system which reflects the extent of local antigenic stimulation.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Pappo J,Thomas WD Jr,Kabok Z,Taylor NS,Murphy JC,Fox JGdoi
10.1128/IAI.63.4.1246-1252.1995subject
Has Abstractpub_date
1995-04-01 00:00:00pages
1246-52issue
4eissn
0019-9567issn
1098-5522journal_volume
63pub_type
杂志文章abstract::The binding of (125)I-labeled S component to rabbit polymorphonuclear leukocytes was found to be concentration dependent and saturable at 37 degrees C. Scatchard analysis of the binding curve gave a straight line, indicating that S component binds to a single population of sites. The dissociation constant, K(D), deriv...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.2.362-367.1981
更新日期:1981-11-01 00:00:00
abstract::One aspect in a broad spectrum of possible mechanisms of cariostatic reactions of fluoride is its interaction with the metabolism of oral bacteria. Information on the mechanisms and kinetics of fluoride inhibition of essential enzymes of the glycolytic pathway of the relevant bacteria is lacking. In this work, the iso...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.4.1043-1047.1990
更新日期:1990-04-01 00:00:00
abstract::A hallmark of Bartonella henselae is persistent bacteremia in cats despite the presence of a vigorous host immune response. To understand better the long-term survival of B. henselae in cats, we examined the feline humoral immune response to B. henselae outer membrane (OM) proteins in naturally and experimentally infe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.6.3097-3105.2004
更新日期:2004-06-01 00:00:00
abstract::The ability of Streptococcus salivarius strain TOVE-R to inhibit the ecological emergence of virulent representatives of the most prevalent human mutans streptococci on the teeth of specific pathogen-free Osborne-Mendel rats was studied. Rats which were infected by TOVE-R, or either S. mutans 10449S or S. sobrinus 671...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.49.1.76-83.1985
更新日期:1985-07-01 00:00:00
abstract::Dissemination of Leishmania within the host is related to parasites undergoing unchecked proliferation. We therefore studied the effects of oxidant generating systems on promastigote multiplication by (i) direct determinations of organism proliferation and (ii) the incorporation of [3H]uracil into promastigote nucleop...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.36.3.1023-1027.1982
更新日期:1982-06-01 00:00:00
abstract::The protection and immune response to infection caused by the parasite Schistosoma mansoni were studied by comparison of two murine irradiated-vaccine models. Mice were exposed from 1 to 4 times to infective-stage cercariae attenuated with either a moderate dose (15 kilorads) or a high dose (50 kilorads) of radiation....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.1.90-94.1992
更新日期:1992-01-01 00:00:00
abstract::Oral inoculation of 5-day-old gnotobiotic pigs with Salmonella enterica serovar Typhimurium strain F98 resulted in severe enteritis and invasive disease. Preinoculation 24 h earlier with an avirulent mutant of Salmonella enterica serovar Infantis (1326/28) completely prevented disease for up to 14 days (when the exper...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.4.2182-2191.2003
更新日期:2003-04-01 00:00:00
abstract::Studies concerned with the mechanisms responsible for relative resistance or susceptibility of strains of inbred mice to Trypanosoma musculi infections are presented. Treatment with 400 rads of ionizing radiation, silica dust, or trypan blue (reticuloendothelial blocking agents) rendered C3H mice unable to control the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.6.1647-1655.1989
更新日期:1989-06-01 00:00:00
abstract::Staphylococcus aureus infections are an important public health concern due to their increasing incidence and high rates of mortality. The success of S. aureus as a pathogen is highly related to its enormous capacity to evade the host immune response. The critical role of tumor necrosis factor alpha (TNF-α) in the ini...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00593-13
更新日期:2013-11-01 00:00:00
abstract::Studies conducted with F1 and F2 progeny of crosses between strains of inbred mice that differ greatly in their capacity to make an antibody response to type III pneumococcal polysaccharide, dextran B-1355, and lipopolysaccharide from Escherichia coli 0113 have shown that multiple genes influence the magnitude of the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.1.56-61.1984
更新日期:1984-07-01 00:00:00
abstract::Clostridium difficile toxins A and B (TcdA and TcdB) are the causative agents of antibiotic-associated pseudomembranous colitis. Mucosal mast cells play a crucial role in the inflammatory processes underlying this disease. We studied the direct effects of TcdA and TcdB on the human mast cell line HMC-1 with respect to...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00195-07
更新日期:2007-08-01 00:00:00
abstract::Trichinella spiralis creates a unique intracellular habitat in striated muscle. We report that a proteoglycan, syndecan-1, is induced early in infection yet is not essential for habitat development and exerts a modest influence on the immune response. This report is the first to address the requirement for a specific ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.3.1941-1943.2006
更新日期:2006-03-01 00:00:00
abstract::Rhodococcus equi is a facultative intracellular pathogen of macrophages, relying on the presence of a conjugative virulence plasmid harboring a 21-kb pathogenicity island (PAI) for growth in host macrophages. The PAI encodes a family of 6 virulence-associated proteins (Vaps) in addition to 20 other proteins. The contr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00230-15
更新日期:2015-08-01 00:00:00
abstract::By subcutaneous treatment with an aqueous solution of 6-O-stearoyl-N-acetylmuramyl-L-alanyl-D-isoglutamine [6-O-CH3-(CH2)16-CO-MurNAc-L-Ala-D-isoGln] [referred to here as L18-MDP(Ala)], an augmentation of the resistance of mice to Escherichia coli, Pseudomonas aeruginosa. Staphylococcus aureus, and Candida albicans in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.1.292-300.1982
更新日期:1982-07-01 00:00:00
abstract::Previous experimentation has highlighted a number of difficulties in the development of carrier-based bivalent vaccines (J.-F. Viret and D. Favre, Biologicals 22:361-372, 1994) In an attempt to obviate these carrier strains. Toward this aim, a series of defined rfbInaba deletion (delta rfbInaba) mutants of the cholera...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.2.576-584.1996
更新日期:1996-02-01 00:00:00
abstract::We previously reported that two surface-exposed high-molecular-weight proteins, HMW1 and HMW2, expressed by a prototypic strain of nontypeable Haemophilus influenzae (NTHI) mediate attachment to human epithelial cells. These proteins are members of a family of highly immunogenic proteins common to most nontypeable Hae...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.8.3032-3037.1996
更新日期:1996-08-01 00:00:00
abstract::A purified A-antigen preparation of Blastomyces dermatitidis was determined to be composed of five major glycoprotein bands, visible with Coomassie blue and periodic acid-Schiff staining of polyacrylamide gels. At least 20 additional protein bands were detected by using a silver stain, which was 100 times more sensiti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.33.1.171-177.1981
更新日期:1981-07-01 00:00:00
abstract::The secretion of aspartic proteases (Saps) has long been recognized as a virulence-associated trait of the pathogenic yeast Candida albicans. In this study, we report that different recombinant Saps, including Sap1, Sap2, Sap3, and Sap6, have differing abilities to induce secretion of proinflammatory cytokines by huma...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00789-10
更新日期:2010-11-01 00:00:00
abstract::Mycobacterium bovis BCG is widely used as a vaccine against tuberculosis (TB), despite its variable protective efficacy. Relatively little is known about the immune response profiles following BCG vaccination in relation to protection against TB. Here we tested whether BCG vaccination results in immune responses to Do...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01999-06
更新日期:2007-07-01 00:00:00
abstract::The presence of choriogonadotropin- and alpha-subunit-like materials in two species of bacteria identified as Staphylococcus simulans and Streptococcus faecalis have been demonstrated by the indirect fluorescein-labeled and the indirect peroxidase-labeled immunocytochemical techniques, utilizing antiserum for human ch...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.1.487-494.1981
更新日期:1981-01-01 00:00:00
abstract::Ingestion of 14C-amino acid-labeled Chlamydia psittaci (6BC) by mouse fibroblasts (L cells) was inhibited when the host cells were incubated for 30 min at 37 degrees C in Earle salts containing 10 mug of crystalline trypsin per ml. Tryptic digestion also inhibited the ingestion of 1-mum polystrene latex beads. Trypsin...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.14.3.645-651.1976
更新日期:1976-09-01 00:00:00
abstract::Binding of Escherichia coli strain 431 heat-stable enterotoxin (STa) and activation of intestinal particulate guanylate cyclase by E. coli STa were studied with rat intestinal epithelial cells and brush border membranes (BBMs). The rates of guanylate cyclase stimulation by 431 STa in cells and BBMs were rapid, with ma...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.46.2.537-543.1984
更新日期:1984-11-01 00:00:00
abstract::Purified cell walls representing a wide variety in teichoic acid and peptidoglycan structure prepared from eight different gram-positive bacterial species induced the production of tumor necrosis factor alpha (TNF-alpha) and interleukin-6 from human monocytes in the presence of 10% plasma or serum. Significant amounts...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.7.2715-2721.1994
更新日期:1994-07-01 00:00:00
abstract::A DNA microarray containing fragments of 137 Borrelia burgdorferi B31 putative lipoprotein genes was used to examine Lyme disease spirochetes. DNA from B. burgdorferi sensu stricto B31, 297, and N40; Borrelia garinii IP90; and Borrelia afzelii P/Gau was fluorescently labeled and hybridized to the microarray, demonstra...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.6.3300-3303.2002
更新日期:2002-06-01 00:00:00
abstract::Toxigenic Vibrio cholerae strains are lysogens of CTXPhi, a filamentous phage which encodes cholera toxin. The receptor for CTXPhi for invading V. cholerae cells is the toxin-coregulated pilus (TCP), the genes for which reside in a larger genetic element, the TCP pathogenicity island. We analyzed 146 CTX-negative stra...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.12.5819-5825.1998
更新日期:1998-12-01 00:00:00
abstract::Toll-like receptor 4 (TLR4), which recognizes lipopolysaccharide from Gram-negative bacteria, plays a major role in resistance of mice and humans to Salmonella infection. In chickens, Salmonella may establish a carrier state whereby bacteria are able to persist in the host organism without triggering clinical signs. B...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00025-11
更新日期:2011-08-01 00:00:00
abstract::Mutant analysis was used to identify Moraxella catarrhalis gene products necessary for biofilm development in a crystal violet-based assay involving 24-well tissue culture plates. The wild-type M. catarrhalis strains that formed the most extensive biofilms in this system proved to be refractory to transposon mutagenes...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.3.1588-1596.2006
更新日期:2006-03-01 00:00:00
abstract::Development of an effective vaccine against Leishmania infection is a priority of tropical disease research. We have recently demonstrated protection against Leishmania major in the murine and nonhuman primate models with individual or combinations of purified leishmanial recombinant antigens delivered as plasmid DNA ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.8.4215-4225.2002
更新日期:2002-08-01 00:00:00
abstract::Infection of CFW mice with Trichinella spiralis induced a state of relative unresponsiveness to passive cutaneous anaphylaxis (PCA) induced with hen egg albumin and its corresponding antibodies. The unresponsiveness was to PCA produced either with immunoglobulin G1 (IgG1) or IgE type of antibodies, but was more pronou...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.1.84-90.1977
更新日期:1977-01-01 00:00:00
abstract::The combining sites of type 1 fimbrial lectins of various species of enterobacteria were studied by measuring the inhibitory activity of linear and branched oligosaccharides and several glycosides of D-mannose on the agglutination of yeast cells by the organisms. The results showed that all five strains of Escherichia...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.3.1088-1090.1984
更新日期:1984-03-01 00:00:00