Abstract:
:The presence of choriogonadotropin- and alpha-subunit-like materials in two species of bacteria identified as Staphylococcus simulans and Streptococcus faecalis have been demonstrated by the indirect fluorescein-labeled and the indirect peroxidase-labeled immunocytochemical techniques, utilizing antiserum for human choriogonadotropin, for its alpha and beta-subunits and the bera-subunit COOH-terminal peptide. The bacteria were originally isolated from the urine of two patients with advanced forms of cancer. Chromatography done on the water-soluble extract of acetone powder preparations of the bacterial cultures revealed the presence of a material similar to the complete trophoblastic hormone and to its beta-subunit in the culture media of S. simulans, and to the beta-subunit in the media of S. faecalis. No free alpha-subunit was detectable. Furthermore, the choriogonadotropin-like factor demonstrated biological activity in in vivo assay systems. From the present results, it can be concluded that some species of "cancer-associated" bacteria can synthesize a human trophoblastic hormone-like glycoprotein with physicochemical properties similar to those of the human trophoblastic hormone that is biologically active and that is either released complete or as one of its subunits in the culture media.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Acevedo HF,Koide SS,Slifkin M,Maruo T,Campbell-Acevedo EAdoi
10.1128/IAI.31.1.487-494.1981subject
Has Abstractpub_date
1981-01-01 00:00:00pages
487-94issue
1eissn
0019-9567issn
1098-5522journal_volume
31pub_type
杂志文章abstract::Borrelia burgdorferi, the causative agent of Lyme disease, has a complex genome consisting of a linear chromosome and up to 21 linear and circular plasmids. These plasmids encode numerous proteins critical to the spirochete's infectious cycle and many hypothetical proteins whose functions and requirements are unknown....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01059-09
更新日期:2010-06-01 00:00:00
abstract::We identified nonhemolytic mutants of Listeria monocytogenes that were severely deficient in their ability to invade mammalian nonprofessional phagocytes. These mutants were generated spontaneously or by means of transposon Tn916 mutagenesis. In terms of their extracellular proteins, the noninvasive mutants were defic...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.12.3988-3995.1990
更新日期:1990-12-01 00:00:00
abstract::Clostridium difficile is recognized as the major cause of antibiotic-associated colitis. C. difficile produces two toxins, A (enterotoxin) and B (cytotoxin), that are implicated in the pathogenesis of the colitis. We examined the dose responses, time course, and synergism of these two toxins in ligated rabbit intestin...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.3.582-588.1988
更新日期:1988-03-01 00:00:00
abstract::The intravascular trematode Schistosoma mansoni is a causative agent of schistosomiasis, a disease that constitutes a major health problem globally. In this study we cloned and characterized the schistosome tegumental phosphodiesterase SmNPP-5 and evaluated its role in parasite virulence. SmNPP-5 is a 52.5-kDa protein...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05431-11
更新日期:2011-10-01 00:00:00
abstract::Bartonella bacilliformis is the etiologic agent of Oroya fever in humans. Flagellum-mediated motility has been postulated as a major virulence factor for invasion of host cells. To address this hypothesis, we purified and characterized flagella from strain KC584 and then assessed their role in human erythrocyte associ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.12.4962-4971.1993
更新日期:1993-12-01 00:00:00
abstract::In this study, the role of the hepatobiliary system in the early pathogenesis of Salmonella enteritidis infection was investigated in a rat model. Intravenous (i.v.) challenge with lipopolysaccharide (LPS) has previously been shown to enhance the translocation of normal gut flora. We first confirmed that LPS can simil...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.1.1-5.2000
更新日期:2000-01-01 00:00:00
abstract::Escherichia coli strains expressing the K1 capsule are a major cause of sepsis and meningitis in human neonates. The development of these diseases is dependent on the expression of a range of virulence factors, many of which remain uncharacterized. Here, we show that all but 1 of 34 E. coli K1 neonatal isolates carrie...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00716-15
更新日期:2015-09-01 00:00:00
abstract::Streptococcus pneumoniae is a major cause of pneumonia and meningitis. Airway colonization is a necessary precursor to disease, but little is known about how the bacteria establish and maintain colonization. Carbohydrates are required as a carbon source for pneumococcal growth and, therefore, for colonization. Free ca...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00832-10
更新日期:2011-03-01 00:00:00
abstract::Peptidoglycan from a Staphylococcus epidermidis strain, isolated from a patient with septicemia, was preincubated with human serum. This mixture was then investigated for its potency to induce tumor necrosis factor (TNF) secretion by human blood monocytes. TNF was measured in the supernatants by using a bioassay and/o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.9.3837-3843.1994
更新日期:1994-09-01 00:00:00
abstract::A set of strain- and size-variant highly immunogenic membrane surface protein antigens of Mycoplasma bovis, which has been identified by a monoclonal antibody, is shown in this report to make up a family of antigenically and structurally related lipid-modified proteins, designated Vsps (variable surface proteins). By ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.11.5075-5084.1994
更新日期:1994-11-01 00:00:00
abstract::Ultrastructural immunoperoxidase studies were done in spinal cords of mice infected with wild type vesicular stomatitis virus or its temperature-sensitive (ts) mutant G31. Infected neurons showed subplasmalemmal staining of viral antigen and staining of viral particles budding from the neuronal membrane in wild-type v...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.1.276-281.1979
更新日期:1979-04-01 00:00:00
abstract::Shiga toxin-producing Escherichia coli (STEC) is a food-borne pathogen with a low infectious dose that colonizes the colon in humans and can cause severe clinical manifestations such as hemolytic-uremic syndrome. The urease enzyme, encoded in the STEC chromosome, has been demonstrated to act as a virulence factor in o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00210-12
更新日期:2012-08-01 00:00:00
abstract::The role of CD8 T cells in controlling Mycobacterium tuberculosis infections in mice was confirmed by comparing the levels of growth of the organism in control, major histocompatibility complex class II knockout, and athymic mice and by transferring T-cell populations into athymic mice. By using donor mice which were ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.2.830-834.1998
更新日期:1998-02-01 00:00:00
abstract::The structural gene of pneumolysin was randomly mutagenized. A screen, developed to identify mutants with reduced hemolytic activity, identified substitutions within the cysteine-containing region and also at residues toward the N terminus of the toxin. These are the first reported changes within the N-terminal region...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.2.757-758.1994
更新日期:1994-02-01 00:00:00
abstract::A method was developed to study the adhesion of Streptococcus pneumoniae to human pharyngeal epithelial cells. Epithelial cells from healthy persons, pneumococcal strains from patients with otitis media, meningitis, or septicemia, and pneumococcal cells from the nasopharynx of healthy carriers were used. Adhesion was ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.32.1.311-317.1981
更新日期:1981-04-01 00:00:00
abstract::To determine the protective efficacy of human hyperimmune globulin to Haemophilus influenzae type b disease in an infant rat model, we compared hyperimmune globulin containing 600 mug of anti-polyribophosphate (PRP) antibody per ml to conventional immune globulin containing 66 mug of anti-PRP antibody per ml. The hype...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.2.709-714.1983
更新日期:1983-02-01 00:00:00
abstract::Cell-mediated immunity to varicella-zoster (V-Z) virus in persons immune to varicella has been demonstrated, using a tissue culture technique. Cell-mediated immunity was reflected by the ability of peripheral leukocytes (lymphocytes and monocytes) from human donors to inactivate V-Z virus. Leukocytes were stimulated b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.6.1549-1553.1976
更新日期:1976-06-01 00:00:00
abstract::To investigate the correlation between the incA I47T mutation in Chlamydia trachomatis and the nonfusogenic phenotype, the incA genes of 25 isolates were sequenced. Four major sequence types were identified. Seven isolates (28%) had the I47T mutation. Isolates representing the four sequence types expressed IncA in the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.7.4654-4656.2001
更新日期:2001-07-01 00:00:00
abstract::Strains of Bacteroides fragilis that produce a ca. 20-kDa heat-labile protein toxin (termed B. fragilis toxin [BFT]) have been associated with diarrheal disease of animals and humans. BFT alters the morphology of intestinal epithelial cells both in vitro and in vivo and stimulates secretion in ligated intestinal segme...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.3.1007-1013.1997
更新日期:1997-03-01 00:00:00
abstract::Mannan (MAN) extracted from Candida albicans 20A was investigated for its potential as an antigen in the detection of cell-mediated immunity (CMI) in vivo and in vitro and for its ability to modulate CMI when administered intravenously (i.v.). CBA/J mice were either immunized as adults by the cutaneous inoculation of ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.3.693-700.1989
更新日期:1989-03-01 00:00:00
abstract::Tuberculosis is rising in the developing world due to poor health care, human immunodeficiency virus type 1 infection, and the low protective efficacy of the Mycobacterium bovis BCG vaccine. A new vaccination strategy that could protect adults in the developing world from tuberculosis could have a huge impact on publi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.6.3814-3816.2005
更新日期:2005-06-01 00:00:00
abstract::Staphylococcus aureus is an important opportunistic pathogen and is the etiological agent of many hospital- and community-acquired infections. The golden pigment, staphyloxanthin, of S. aureus colonies distinguishes it from other staphylococci and related Gram-positive cocci. Staphyloxanthin is the product of a series...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00838-16
更新日期:2017-01-26 00:00:00
abstract::The suspected correlation between cessation of type C virus production and halt in immunoglobulin secretion by murine myeloma cells was studied. Employing two variants of the murine myeloma MOPC-315, immunoglobulin A-producing and nonproducing cells, we demonstrated that the two myelomas release similar levels of type...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.25.2.569-573.1979
更新日期:1979-08-01 00:00:00
abstract::Helicobacter mustelae is a urease-rich bacterium associated with gastritis in ferrets. The ureases of H. mustelae and Helicobacter pylori, a bacterium implicated in human gastritis, share many characteristics. Helicobacter sp. ureases appear to be unique among bacterial enzymes in exhibiting submillimolar Km values an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.9.3343-3345.1991
更新日期:1991-09-01 00:00:00
abstract::The role of a plasmid in the virulence activity of an enteropathogenic Escherichia coli (EPEC) strain belonging to serotype 0111:NM was examined. EPEC strain B171, which is resistant to chloramphenicol, streptomycin, sulfathiazole, and tetracycline, harbors a 54-megadalton plasmid, pYR111, and exhibits localized adher...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.9.2052-2056.1987
更新日期:1987-09-01 00:00:00
abstract::Salmonella enterica serovar Enteritidis is a major cause of food-borne diseases associated with consumption of shell eggs. Clinical isolates of S. enterica serovar Enteritidis exhibit a wide spectrum of virulence in mice. A highly virulent isolate (SE2472) was previously shown to be more resistant in vitro than other ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.2.451-461.2002
更新日期:2002-02-01 00:00:00
abstract::We have previously identified mgrA (rat) as a regulator of autolysis in Staphylococcus aureus. Besides its effect on autolytic activity, we recently found alterations in the expression of regulator and target virulence genes in the mgrA mutant. Northern analysis and transcription fusion assays showed that inactivation...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.3.1423-1431.2005
更新日期:2005-03-01 00:00:00
abstract::An enzyme from Treponema denticola that hydrolyzes a synthetic trypsin substrate, N-alpha-benzoyl-L-arginine-p-nitroanilide (BAPNA), was purified to near homogeneity, as judged by gel electrophoresis. The molecular weight of the enzyme was estimated to be ca. 69,000 by sodium dodecyl sulfate-polyacrylamide gel electro...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.53.1.213-220.1986
更新日期:1986-07-01 00:00:00
abstract::Rinderpest virus infection was shown to induce marked suppression of both humoral antibody response and cell-mediated immunity in rabbits. The virus exhibited a suppressive effect on primary antibody response as indicated by a decrease in numbers of plaque-forming cells (immunoglobulin [Ig]M) and hemagglutinating anti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.2.206-211.1974
更新日期:1974-02-01 00:00:00
abstract::Nonviable Mycobacterium tuberculosis strain Jamaica suspended in oil-droplet emulsions was used to enhance resistance of mice against encephalomyocarditis virus (EMCV). The mycobacteria-injected mice were significantly resistant to 50,000 50% lethal doses of EMCV. Similar concentrations of virus in plasma of normal an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.22.3.740-745.1978
更新日期:1978-12-01 00:00:00