Abstract:
:The role of a plasmid in the virulence activity of an enteropathogenic Escherichia coli (EPEC) strain belonging to serotype 0111:NM was examined. EPEC strain B171, which is resistant to chloramphenicol, streptomycin, sulfathiazole, and tetracycline, harbors a 54-megadalton plasmid, pYR111, and exhibits localized adherence (LA) with HeLa cells. Curing the plasmid yielded strain B171-4, which had lost the ability to exhibit LA, resistance to the antibiotics, and the lipopolysaccharide (LPS) O-antigenic polysaccharide. To confirm that these phenotypic characteristics were specified by pYR111, the plasmid was transferred by conjugation into a nalidixic acid-resistant strain of E. coli HB101. LA and antimicrobial resistance were expressed in most of the transconjugants examined. The O-polysaccharide side chains, antigenically reactive with O111-specific antiserum, were also expressed by the transconjugants. Although EPEC plasmids coding for both drug resistance and LA have been described, an EPEC plasmid encoding the expression of an LPS O antigen has not been previously reported. Similar findings described for some Shigella and Salmonella strains suggest that plasmid-encoded modification of the LPS in some enteric bacterial species may be more common than previously recognized and may contribute to the characteristic virulence activity of the organism.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Riley LW,Junio LN,Libaek LB,Schoolnik GKdoi
10.1128/IAI.55.9.2052-2056.1987subject
Has Abstractpub_date
1987-09-01 00:00:00pages
2052-6issue
9eissn
0019-9567issn
1098-5522journal_volume
55pub_type
杂志文章abstract::Staphylococcus aureus is a pathogen that causes significant morbidity and mortality. Nasal carriage is a major source of transmission and of endogenous infection. Persistent carriage is detected in ∼30% of healthy individuals. While Th17 cells have been shown to play a role in S. aureus infection and clearance, the im...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00834-17
更新日期:2018-02-20 00:00:00
abstract::The enzymes responsible for reducing H2O2 were surveyed in 49 strains of Leptospira by using semiquantitative assays for catalase and peroxidase. The survey revealed a differential distribution of catalase and peroxidase activities between the two leptospiral complexes. The pathogenic Leptospira interrogans strains ga...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.22.3.672-675.1978
更新日期:1978-12-01 00:00:00
abstract::Bacteria exhibit extensive genetic heterogeneity within species. In many cases, these differences account for virulence properties unique to specific strains. Several such loci have been discovered in the genome of the type b serotype of Haemophilus influenzae, a human pathogen able to cause meningitis, pneumonia, and...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.3.1098-1108.2003
更新日期:2003-03-01 00:00:00
abstract::Mouse spleen cultures were infected with murine cytomegalovirus, either before or after separating the cells into various classes, and the growth of virus and production of infectious centers were measured in each cell class. The separation techniques, which were used in various combinations, comprised: adherence to p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.3.853-860.1979
更新日期:1979-12-01 00:00:00
abstract::Spleen cells from BALB/c mice infected with 2 X 10(7) L. major promastigotes and developing progressive disease produced significantly lower levels of interleukin-2 (IL-2) in response to concanavalin A stimulation than did spleen cells from uninfected mice. In contrast, spleen cells from sublethally irradiated and inf...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.2.386-394.1986
更新日期:1986-11-01 00:00:00
abstract::The lymphogranuloma venereum (LGV) and trachoma biovars of Chlamydia trachomatis exhibit differences in biological properties both in vivo and in vitro. To identify analogous biochemical differences, we studied the molecular charges of chlamydial outer membrane proteins (OMPs) by means of isoelectric focusing and none...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.2.488-494.1985
更新日期:1985-11-01 00:00:00
abstract::A Pasteurella haemolytica mutant incapable of producing leukotoxin was created by allelic replacement. Concentrated culture supernatants from wild-type P. haemolytica, but not from the mutant, contained the 102-kDa leukotoxin protein and lysed bovine lymphoma cells and sheep erythrocytes. Wild-type P. haemolytica demo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.8.3209-3212.1995
更新日期:1995-08-01 00:00:00
abstract::The ability of some typical enteropathogenic Escherichia coli (EPEC) strains to adhere to, invade, and increase interleukin-8 (IL-8) production in intestinal epithelial cells in vitro has been demonstrated. However, few studies regarding these aspects have been performed with atypical EPEC (aEPEC) strains, which are e...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00177-09
更新日期:2009-10-01 00:00:00
abstract::Serum antibody responses to toxic shock syndrome (TSS) toxin 1 (TSST-1) and staphylococcal enterotoxins A, B, and C were determined by western blot (immunoblot) analysis of acute- and convalescent-phase paired sera from 18 TSS- and 31 non-TSS-associated Staphylococcus aureus infections. Compared with non-TSS cases, se...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.1.231-234.1989
更新日期:1989-01-01 00:00:00
abstract::In pathogenic bacteria, iron acquisition is important for colonization and proliferation in the host under iron-limited conditions. The ability of Vibrio spp. to acquire iron is often critical to their virulence, causing gastroenteritis or excessive watery diarrhea in humans. In the study described here, we cloned the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.2.722-729.2005
更新日期:2005-02-01 00:00:00
abstract::Newly hatched chicks were vaccinated orally with a genetically defined Salmonella enteritidis aroA candidate, strain CVL30. In chickens immunized with 10(5) or 10(9) CFU and challenged by the intravenous route with 10(8) CFU of S. enteritidis 109 Nalr at 8 weeks old, there were similar reductions in colonization of th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.11.4747-4754.1994
更新日期:1994-11-01 00:00:00
abstract::Recent data suggest that fibronectin may favor Staphylococcus aureus infection by promoting attachment to either injured tissues or implanted foreign bodies. We studied the quantitative adsorption of fibronectin onto polymethylmethacrylate (PMMA) cover slips by using a 125I-labeled preparation of the purified plasma g...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.3.768-774.1984
更新日期:1984-09-01 00:00:00
abstract::Recent evidence suggests that immune-mediated gastric epithelial cell apoptosis through Fas-Fas ligand interactions participates in Helicobacter pylori disease pathogenesis. To define the role of Fas signaling in vivo, H. pylori strain SS1 infection in C57BL/6 mice was compared to that in mice deficient in the Fas lig...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.5.2591-2597.2002
更新日期:2002-05-01 00:00:00
abstract::Q fever has been associated with granulomatous changes in clinical biopsy material obtained from liver and bone marrow. Local reactions to skin testing have been described in previously sensitized humans, but histological studies of such reactions have not been reported. We note that delayed hypersensitivity reactions...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.1.388-393.1983
更新日期:1983-01-01 00:00:00
abstract::Dendritic cells (DC) suffer a maturation defect following interaction with erythrocytes infected with malaria parasites and become unable to induce protective malaria liver-stage immunity. Here we show that, by contrast, maturation-arrested DC in vitro are capable of the successful induction of antigen-specific gamma ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.9.5331-5339.2004
更新日期:2004-09-01 00:00:00
abstract::Minimally manipulated nasal secretions, an accessible form of airway surface fluid, were tested against indigenous and added bacteria by using CFU assays. Antimicrobial activity was found to vary between donors and with different target bacteria and was markedly diminished by dilution of the airway secretions. Donor-t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.7.3267-3275.1999
更新日期:1999-07-01 00:00:00
abstract::FMS-like tyrosine kinase-3 ligand (Flt3L) is a dendritic cell (DC) growth and differentiation factor with potential in antitumor therapies and antibacterial immunization strategies. However, the effect of systemic Flt3L treatment on lung-protective immunity against bacterial infection is incompletely defined. Here, we...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00854-12
更新日期:2012-12-01 00:00:00
abstract::Passive transfer of antibody may be useful for preexposure prophylaxis against biological agents used as weapons of terror, such as Bacillus anthracis. Studies were performed to evaluate the ability of anthrax antiprotective antigen (anti-PA) and antilethal factor (anti-LF) neutralizing monoclonal antibodies (mAbs) to...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00529-07
更新日期:2007-11-01 00:00:00
abstract::Uropathogenic Escherichia coli strain J96 carries multiple determinants for fimbrial adhesins. The regulatory protein PapB of P fimbriae has previously been implicated in potential coregulatory events. The focB gene of the F1C fimbria determinant is highly homologous to papB; the translated sequences share 81% identit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01010-07
更新日期:2008-02-01 00:00:00
abstract::Yersinia pestis KIM5 was found to be cytotoxic for the IC21 and P388D1 mouse macrophage cell lines, as well as for resident peritoneal macrophages from C57BL/6 mice. Affected cells phagocytosed KIM5 inefficiently, became spherical, detached readily from culture dishes, and retained 51Cr poorly. The cytotoxic effect wa...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.51.3.788-794.1986
更新日期:1986-03-01 00:00:00
abstract::Variation has been observed in the structural polypeptides of swine vesicular disease viruses isolated from the United Kingdom and Hong Kong. Despite the limited number of isolates examined, several distinct polypeptide patterns were obtained when the virus structural proteins were examined by polyacrylamide gel elect...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.3.593-599.1979
更新日期:1979-06-01 00:00:00
abstract::Gamma interferon (IFN-gamma)-primed human gingival fibroblasts (HGF) have been shown to produce higher levels of interleukin-8 (IL-8) upon stimulation with bacterial products and inflammatory cytokines than nonprimed controls. In this study, we examined whether priming of HGF with IFN-gamma up-regulates IL-8 productio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.3.1272-1278.2002
更新日期:2002-03-01 00:00:00
abstract::Lysogenic conversion of Shigella flexneri type antigens was studied with the aid of wild-type and thermosensitive mutant phages. With all wild-type phages, the appearance of glycosylated antigen was accompanied by the appearance of polyprenyl phosphate glucose synthetase activity. With some of the mutant phages, the a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.14.6.1290-1292.1976
更新日期:1976-12-01 00:00:00
abstract::The Lyme disease spirochete, Borrelia burgdorferi, controls protein expression patterns during its tick-mammal infection cycle. Earlier studies demonstrated that B. burgdorferi synthesizes 4,5-dihydroxy-2,3-pentanedione (autoinducer-2 [AI-2]) and responds to AI-2 by measurably changing production of several infection-...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00032-15
更新日期:2015-04-01 00:00:00
abstract::Attachment of Fusobacterium nucleatum to various oral surfaces is mediated by several adhesins anchored on its outer surface. Monoclonal antibodies (MAbs) were prepared and used to identify the putative galactose-binding adhesin of F. nucleatum PK1594. Four unique MAbs, 8G7, 26B9, 28G11, and 29D4, were isolated on the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.12.5231-5237.1997
更新日期:1997-12-01 00:00:00
abstract::We have developed a rapid, continuous method for real-time monitoring of biofilms, both in vitro and in a mouse infection model, through noninvasive imaging of bioluminescent bacteria colonized on Teflon catheters. Two important biofilm-forming bacterial pathogens, Staphylococcus aureus and Pseudomonas aeruginosa, wer...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.2.882-890.2003
更新日期:2003-02-01 00:00:00
abstract::Pathogenic species of the spotted fever group Rickettsia are subjected to repeated exposures to the host complement system through cyclic infections of mammalian and tick hosts. The serum complement machinery is a formidable obstacle for bacteria to overcome if they endeavor to endure this endozoonotic cycle. We have ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00349-12
更新日期:2012-08-01 00:00:00
abstract::In this study we found that serum inhibitory activity against Blastomyces dermatitidis was principally mediated by albumin. This was confirmed in experiments using albumin from several mammalian species. Analbuminemic rat serum did not inhibit B. dermatitidis growth in vivo; however, the addition of albumin restored i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.11.6648-6652.2003
更新日期:2003-11-01 00:00:00
abstract::Naturally occurring antibodies against [Gal alpha-1,3-Gal] structures (anti-Gal antibodies) are the primary effectors of human hyperacute rejection (HAR) of nonhuman tissue. Unlike most mammals, humans lack a functional alpha-1,3-galactosyltransferase (GalT) gene and produce abundant anti-Gal antibodies, putatively in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.11.6215-6222.2002
更新日期:2002-11-01 00:00:00
abstract::Tissue macrophages are the obligatory host cells for Leishmania donovani, the causative agent of visceral leishmaniasis. In this study we sought to determine whether activated macrophages, as defined by the functional criterion of tumor cell cytotoxicity, were also able to exert a microbicidal effect on ingested L. do...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.33.3.918-926.1981
更新日期:1981-09-01 00:00:00