Abstract:
:Dissemination of Leishmania within the host is related to parasites undergoing unchecked proliferation. We therefore studied the effects of oxidant generating systems on promastigote multiplication by (i) direct determinations of organism proliferation and (ii) the incorporation of [3H]uracil into promastigote nucleoprotein. These two parameters correlated closely as measures of organism replication as demonstrated by parallel suppression of them by the protein synthesis inhibitors puromycin and cycloheximide and the nucleic acid synthesis inhibitors actinomycin D and mitomycin C. Promastigotes showed dose-related susceptibility to reagent and generated hydrogen peroxide (H2O2) as reflected in quantitatively similar decreases in multiplication and [3H]uracil incorporation. These effects were specific for H2O2 as catalase abrogated the dimunition in multiplication. The generation of superoxide anion by acetaldehyde-xanthine oxidase (10 mU/ml) did not alter promastigote replication or nucleoprotein synthesis. These results indicate that Leishmania donovani promastigotes are damaged by H2O2 and that the incorporation of [3H]uracil into promastigote nucleoprotein may be useful for studying the interaction of this parasite with host effector cells.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Reiner NE,Kazura JWdoi
10.1128/IAI.36.3.1023-1027.1982subject
Has Abstractpub_date
1982-06-01 00:00:00pages
1023-7issue
3eissn
0019-9567issn
1098-5522journal_volume
36pub_type
杂志文章abstract::Two inbred mouse strains, derived from feral founders, are susceptible to experimental leishmaniasis due to Leishmania major and support a disease of a severity intermediate between those observed in strains C57BL/6 and BALB/c. Mice of the MAI strain develop a severe, nonhealing, but nonfatal disease with no resistanc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.8.4603-4611.2004
更新日期:2004-08-01 00:00:00
abstract::A purified A-antigen preparation of Blastomyces dermatitidis was determined to be composed of five major glycoprotein bands, visible with Coomassie blue and periodic acid-Schiff staining of polyacrylamide gels. At least 20 additional protein bands were detected by using a silver stain, which was 100 times more sensiti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.33.1.171-177.1981
更新日期:1981-07-01 00:00:00
abstract::Heart and muscle tissues appear to be specific target organs for the in vivo inhibition of protein synthesis by diphtheria toxin. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.2.5.686-688.1970
更新日期:1970-11-01 00:00:00
abstract::Vitamin D(3) (1,25-dihydroxycholecalciferol) induced the phagocyte oxidative burst and intracellular killing of Salmonella enterica serovar Typhimurium in a phosphatidylinositol 3-kinase-dependent manner. The antimicrobial effect was more pronounced for Salmonella SodCI and SodCII mutants, confirming the role of the p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.9.5312-5315.2002
更新日期:2002-09-01 00:00:00
abstract::Host control of Coxiella burnetii infections is believed to be mediated primarily by activated monocytes/macrophages. The activation of macrophages by cytokines leads to the production of reactive oxygen intermediates (ROI) and reactive nitrogen intermediates (RNI) that have potent antimicrobial activities. The contri...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.11.6666-6675.2004
更新日期:2004-11-01 00:00:00
abstract::A novel immunobiologically active fraction was prepared from a phenol-water extract of Prevotella intermedia ATCC 25611 by Sephadex G-100 column chromatography. The fraction consisted mainly of carbohydrate and protein and was devoid of fatty acid. The fraction showed high-molecular-weight bands (10,000 to 12,000) on ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.11.4531-4538.1997
更新日期:1997-11-01 00:00:00
abstract::Tuberculosis remains a leading killer worldwide, and new approaches for its treatment and prevention are urgently needed. This effort will benefit greatly from a better understanding of gene regulation in Mycobacterium tuberculosis, particularly with respect to this pathogen's response to its host environment. We exam...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.3.1518-1529.2002
更新日期:2002-03-01 00:00:00
abstract::Influenza A/Scotland/74 (H3N2) and A/Victoria/75 (H3N2) cold-adapted (ca) recombinant viruses, prepared by mating the A/Ann Arbor/6/60 (H2N2) ca donor virus and influenza A wild-type virus, were evaluated in adult seronegative volunteers (serum hemagglutination-inhibiting antibody titer, =1:8) for level of attenuati...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.23.2.253-259.1979
更新日期:1979-02-01 00:00:00
abstract::A solid-phase radioimmunoassay method has been developed for the detection of rotavirus in the form of a purified antigen and in stool. The parameters of the radioimmunoassay were examined and optimized to give high sensitivity and same-day results. Compared with electron microscopy, the assay is up to 10 times as sen...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.2.439-444.1977
更新日期:1977-05-01 00:00:00
abstract::Cell surface protein SSP-5 in the oral bacterium Streptococcus gordonii M5 binds human salivary agglutinin in a Ca(2+)-dependent reaction (D.R. Demuth, E.E. Golub, and D. Malamud, J. Biol. Chem. 265:7120-7126, 1990). The region of the gene encoding an N-terminal segment of a related polypeptide (SspA) in S. gordonii D...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.8.3199-3208.1993
更新日期:1993-08-01 00:00:00
abstract::The growth of Eimeria vermiformis within cultured murine fibroblastlike (L-929) or rat epithelial-like (RATEC) cells was inhibited by treatment of the cells with the appropriate recombinant gamma interferon. The effect was apparent as a reduction in both the initial numbers of intracellular sporozoites and, to a much ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.2.580-586.1991
更新日期:1991-02-01 00:00:00
abstract::Female guinea pigs were immunized with viable or UV light-inactivated chlamydiae (agent of guinea pig inclusion conjunctivitis), belonging to the species Chlamydia psittaci, by intravenous, subcutaneous, oral, or ocular routes. All animals were then inoculated vaginally with viable chlamydiae to determine the extent o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.8.2599-2605.1990
更新日期:1990-08-01 00:00:00
abstract::We have developed experimental murine Campylobacter infection models which demonstrate efficient establishment and reproducible, high-level colonization. Following oral inoculation, wild-type C3H mice with normal enteric flora were colonized inconsistently and inefficiently by C. jejuni strain 81-176. However, C3H mic...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01094-05
更新日期:2006-09-01 00:00:00
abstract::Periodontitis is the inflammatory response in periodontal tissues elicited by bacterial colonization in periodontal pockets. In this response, pocket epithelial cells are the first cells to come into contact with bacteria. To elucidate this mechanism, we determined the adherence of the periodontopathic bacterium Eiken...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.1.384-394.1999
更新日期:1999-01-01 00:00:00
abstract::Mouse spleen cells which normally cannot support the in vivo replication of vesicular stomatitis virus (VSV) became susceptible to VSV infection after the intraperitoneal growth of certain syngeneic and allogeneic tumors. After 3 days' growth of P815 tumor cells in syngeneic DBA/2 mice, the viral-permissive state for ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.32.3.1014-1023.1981
更新日期:1981-06-01 00:00:00
abstract::Mutation of tryptophan-101 in Clostridium difficile toxin A, a 308-kDa glucosyltransferase, resulted in a 50-fold-reduced cytopathic activity in cell culture experiments. The mutant toxin A was characterized and applied to distinguish between glucosyltransferase-dependent and -independent effects with respect to RhoB ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00545-06
更新日期:2006-10-01 00:00:00
abstract::Bacillary angiomatosis (BA), one of the many clinical manifestations resulting from infection with the facultative intracellular bacterium Bartonella henselae, is characterized by angiogenic lesions. Macrophages have been identified as important effector cells contributing to the angiogenic process during B. henselae ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.9.5735-5742.2005
更新日期:2005-09-01 00:00:00
abstract::Zoonotic infections caused by Salmonella enterica serovar Typhimurium pose a constant threat to consumer health, with the pig being a particularly major source of multidrug-resistant isolates. Vaccination, as a promising approach to reduce colonization and shedding, has been scarcely used, as it interferes with curren...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01908-06
更新日期:2007-05-01 00:00:00
abstract::In the present study, we have investigated the evolution and impact on virulence of a 350-kb genomic duplication present in the most recently evolved members of the Mycobacterium tuberculosis East Asian lineage. In a mouse model of infection, comparing HN878 subclones HN878-27 (no duplication) and HN878-45 (with the 3...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01791-14
更新日期:2014-07-01 00:00:00
abstract::Nonspecific cross-reacting antigen (NCA) is a well-characterized membrane glycoprotein on granulocytes, macrophages, and lung epithelium. Structural studies at the protein and genomic levels have revealed that NCA is a member of the immunoglobulin supergene family, and hybridization studies showed that the transcript ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.7.2485-2493.1991
更新日期:1991-07-01 00:00:00
abstract::We have previously identified two K88ac adhesion receptors (210 and 240 kDa) which are present in membrane preparations from adhesive but not nonadhesive porcine intestinal brush border cells; these adhesin receptors are postulated to be important determinants of the susceptibility of pigs to K88ac+ enterotoxigenic Es...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.12.5404-5410.1994
更新日期:1994-12-01 00:00:00
abstract::The Bordetella pertussis BrkA protein protects against the bactericidal activity of complement and antibody; however, some individuals mount an immune response that overcomes this bacterial defense. To further characterize this process, the bactericidal activities of sera from 13 adults with different modes of exposur...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.3.1424-1431.1999
更新日期:1999-03-01 00:00:00
abstract::Platelets aggregate in response to an adhesin and the platelet aggregation-associated protein (PAAP) expressed on the cell surfaces of certain strains of Streptococcus sanguis. We sought to identify the corresponding PAAP receptor and accessory adhesin binding sites on platelets. Since the adhesion(s) of S. sanguis fo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.9.3628-3633.1995
更新日期:1995-09-01 00:00:00
abstract::Depressed Th1 responses are a prominent feature of human tuberculosis, but an enhanced Th2 response has not been detected in peripheral blood T cells stimulated in vitro with Mycobacterium tuberculosis. In disease due to Mycobacterium leprae, Th2 cells predominate in tissue lesions of patients with extensive disease b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.4.1351-1356.1996
更新日期:1996-04-01 00:00:00
abstract::Salmonella enterica serovar Typhimurium employs two different type III secretion systems (TTSS) encoded within Salmonella pathogenicity islands 1 and 2 (SPI1 and SPI2) for targeting of effector proteins into the cytosol of eukaryotic cells during different stages of the infection cycle. The SPI1 TTSS translocates viru...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.1.334-341.2005
更新日期:2005-01-01 00:00:00
abstract::The effect of bile on the expression of cholera toxin (CT) and the major subunit of the toxin-coregulated pilus (TcpA) and on motility was examined in the Vibrio cholerae O1 classical-biotype strains 0395 and 569B. Although the motility of the cells increased significantly in the presence of bile, transcription of the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.3.1131-1134.1997
更新日期:1997-03-01 00:00:00
abstract::We recently determined that passive transfer of serum directed against a synthetic peptide called LB1 or a recombinant fusion protein immunogen [LPD-LB1(f)(2,1,3)] could prevent otitis media after challenge with a homologous nontypeable Haemophilus influenzae (NTHI) isolate. NTHI residing in the nasopharynx was rapidl...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.5.2756-2765.2000
更新日期:2000-05-01 00:00:00
abstract::In the enteric pathogen Vibrio cholerae, expression of the major virulence factors is controlled by the hierarchical expression of several regulatory proteins comprising the ToxR regulon. In this study, we demonstrate that disruption of the fadD gene encoding a long-chain fatty acyl coenzyme A ligase has marked effect...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00663-10
更新日期:2011-01-01 00:00:00
abstract::Hydrogen sulfide (H(2)S) is a major metabolic end product detected in deep periodontal pockets that is produced by resident periodontopathic microbiota associated with the progression of periodontitis. Treponema denticola, a member of the subgingival biofilm at disease sites, produces cystalysin, an enzyme that catabo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.3.1113-1120.2002
更新日期:2002-03-01 00:00:00
abstract::The Yersinia enterocolitica gsrA gene is a stress protein gene which was originally identified as essential for protecting cells under both extracellular environmental stress and intracellular stress in macrophages due to phagocytosis. The gsrA gene was shown to be a member of the htrA class of genes and to possess a ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.6.2190-2196.1997
更新日期:1997-06-01 00:00:00