Abstract:
:A novel immunobiologically active fraction was prepared from a phenol-water extract of Prevotella intermedia ATCC 25611 by Sephadex G-100 column chromatography. The fraction consisted mainly of carbohydrate and protein and was devoid of fatty acid. The fraction showed high-molecular-weight bands (10,000 to 12,000) on deoxycholate polyacrylamide gel electrophoresis (DOC-PAGE) and was scarcely active in a Limulus test. We designated the fraction Prevotella glycoprotein (PGP). The PGP fraction showed strong mitogenicity on splenocytes and cytokine-inducing activities on peritoneal macrophages from both C3H/HeJ and C3H/HeN mice, and it stimulated human gingival fibroblasts to produce cytokines. The activities of the PGP fraction were resistant to heat inactivation (100 degrees C for 1 h) and protease treatments and were scarcely inhibited by polymyxin B. In contrast, the purified lipopolysaccharide fraction (LPS-PCP) extracted from the same bacterium with a phenol-chloroform-petroleum ether mixture, which showed strong Limulus activity and a single low-molecular-weight band (approximately 3,000) on DOC-PAGE, lacked the activities on splenocytes and macrophages from C3H/HeJ mice and human gingival fibroblasts. The activities of the LPS-PCP fraction on cells from C3H/HeN mice were completely inhibited by polymyxin B. The LPS extracted from the same bacterium with hot phenol-water (LPS-PW) exhibited the properties of both the PGP fraction and the LPS-PCP fraction. These findings suggest that the unique bioactivities of the LPS-PW fraction of oral black-pigmented bacteria reported to date, which differed from those of the classical endotoxin, were derived from the PGP fraction and not from the LPS itself.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Iki K,Kawahara K,Sawamura S,Arakaki R,Sakuta T,Sugiyama A,Tamura H,Sueda T,Hamada S,Takada Hdoi
10.1128/IAI.65.11.4531-4538.1997subject
Has Abstractpub_date
1997-11-01 00:00:00pages
4531-8issue
11eissn
0019-9567issn
1098-5522journal_volume
65pub_type
杂志文章abstract::Gonococci from opaque colonies have cell wall outer membrane proteins that are lacking from organisms which form transparent colonies. These "colony opacity-associated" proteins are among a group of "minor" proteins that exhibit heat modification of their apparent subunit molecular sizes, are easily extracted by deoxy...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.28.1.54-64.1980
更新日期:1980-04-01 00:00:00
abstract::Studies were carried out to determine whether treatment of mice with the synthetic adjuvant muramyl dipeptide afforded any resistance to infection with the obligate intracellular protozoan Toxoplasma gondii. Marked resistance to lethal challenge infection was observed in CBA but not C57BL/6 mice pretreated with muramy...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.2.716-722.1981
更新日期:1981-02-01 00:00:00
abstract::As part of a study of virulence gene regulation in Streptococcus pneumoniae, we have identified a gene encoding a homologue of the staphylococcal catabolite control protein CcpA in the pneumococcal genome sequence. The pneumococcal protein, designated RegM, has significant similarity to members of the LacI/GalR family...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.10.5454-5461.2002
更新日期:2002-10-01 00:00:00
abstract::Preliminary studies established methods for obtaining maximum yields of viable cells. Liquid shaken cultures of Histoplasma capsulatum provided a maximum of 4 x 10(8) to 5 x 10(8) cells/ml regardless of the inoculum size. Under optimum conditions, cells were viable (90 to 95%) for 168 to 240 hr. Generation times range...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.4.2.116-125.1971
更新日期:1971-08-01 00:00:00
abstract::The Corynebacterium diphtheriae hmuO gene encodes a heme oxygenase that is involved in the utilization of heme as an iron source. Transcription of hmuO is activated by heme or hemoglobin and repressed by iron and DtxR. Previous studies with Escherichia coli showed that heme-dependent transcriptional activation of an h...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.11.7406-7412.2005
更新日期:2005-11-01 00:00:00
abstract::Very little is known about the host response to chlamydial genital infection in the male, particularly about the nature of the local response in the urethra. In this study, the pathological and immunologic responses to urethral infection of the male guinea pig with Chlamydia caviae (Chlamydophila caviae) were characte...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01339-09
更新日期:2010-04-01 00:00:00
abstract::Haemophilus ducreyi expresses 2 OmpA homologs, designated MOMP and OmpA2, whose genes are arranged in tandem on the chromosome. Northern blot analysis indicated that momp and ompA2 are transcribed independently. Sequences of the momp open reading frame (ORF) lacking the transcriptional start site were amplified by PCR...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.5.2602-2607.2000
更新日期:2000-05-01 00:00:00
abstract::Actinobacillus pleuropneumoniae, the causative agent of swine pleuropneumonia, secretes a hemolytic activity which is thought to be a factor involved in the pathogenesis of the disease. The biosynthesis of hemolysin by serotype 1 strain 4074 was strongly dependent on the activity of free Ca2+ in the growth medium. At ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.10.2570-2575.1988
更新日期:1988-10-01 00:00:00
abstract::Antigen 2 (Ag2), a major immunoreactive component of Coccidioides immitis mycelium- and spherule-phase cell walls, was recently cloned in our laboratory and was shown to elicit T-cell responses in Coccidioides-immune mice. In this investigation, we evaluated recombinant Ag2 (rAg2) and PCR-generated Ag2 truncations for...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.8.3376-3380.1997
更新日期:1997-08-01 00:00:00
abstract::Cryptococcal meningoencephalitis develops as a result of hematogenous dissemination of inhaled Cryptococcus neoformans from the lung to the brain. The mechanism(s) by which C. neoformans crosses the blood-brain barrier (BBB) is a key unresolved issue in cryptococcosis. We used both an in vivo mouse model and an in vit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.9.4985-4995.2004
更新日期:2004-09-01 00:00:00
abstract::CD14, the leukocyte receptor for lipopolysaccharide (LPS), is important in the response of human polymorphonuclear leukocytes (PMNs) to infection with gram-negative bacteria. The level of CD14 on the PMN surface increases after exposure to some inflammatory stimuli such as N-formyl-methionyl-leucyl-phenylalanine (fMLP...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.11.4747-4753.1997
更新日期:1997-11-01 00:00:00
abstract::The effect of the secondary infection with Toxoplasma gondii on antibody responses to unrelated antigens was examined in mice. A reinfection with 5 X 10(3) organisms did not affect either primary anti-sheep erythrocyte antibody responses, or anti-dinitrophenol antibody responses to dinitrophenylated keyhole limpet hem...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.48.3.686-689.1985
更新日期:1985-06-01 00:00:00
abstract::The American Heart Association supports an association between periodontal diseases and atherosclerosis but not a causal association. This study explores the use of the integrin β6(-/-) mouse model to study the causality. We investigated the ability of a polymicrobial consortium of Porphyromonas gingivalis, Treponema ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01077-15
更新日期:2015-12-01 00:00:00
abstract::Group A streptococci (GAS) are highly prevalent human pathogens whose primary ecological niche is the superficial epithelial layers of the throat and/or skin. Many GAS strains with a strong tendency to cause pharyngitis are distinct from strains that tend to cause impetigo; thus, genetic differences between them may c...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00374-17
更新日期:2017-10-18 00:00:00
abstract::Toxin A (TcdA) and toxin B (TcdB) are major virulence factors of Clostridium difficile. These two toxins intoxicate cultured cells by similar mechanisms, and TcdB generally is more potent than TcdA in cultured cells. The exact reason for this difference is unclear. Here, we report that the cellular effects of TcdA can...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01577-08
更新日期:2009-06-01 00:00:00
abstract::T cell-independent antibody responses develop rapidly, within 3 to 4 days, and are critical for preventing blood-borne pathogens from evolving into life-threatening infections. The interaction of BAFF, also known as BLyS, with its receptors BAFFR and TACI on B cells is critical for B cell homeostasis and function. Usi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01147-13
更新日期:2014-01-01 00:00:00
abstract::A heterogeneous subset of extraintestinal pathogenic Escherichia coli (ExPEC) strains, referred to as uropathogenic E. coli (UPEC), causes most uncomplicated urinary tract infections. However, no core set of virulence factors exists among UPEC strains. Instead, the focus of the analysis of urovirulence has shifted to ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01608-14
更新日期:2014-09-01 00:00:00
abstract::To explore the molecular basis of the T-cell-mediated immune response to Listeria monocytogenes, we cloned and expressed listerial antigens in Escherichia coli using the lambda-ZAP bacteriophage and Bluescript plasmid vectors. A two-stage screening strategy was implemented to identify T-cell-reactive antigens; the fir...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.9.2792-2803.1990
更新日期:1990-09-01 00:00:00
abstract::HbhA of Mycobacterium tuberculosis is a multifunctional binding protein, binding to both sulfated sugars such as heparin and to human complement component C3. HbhA may therefore interact with host molecules and/or host cells during M. tuberculosis infection and play a role in the pathogenesis of this bacterium. The pu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.12.6751-6760.2002
更新日期:2002-12-01 00:00:00
abstract::Monoclonal antibodies (MAbs) were raised against native and denatured alpha-hemolysin (HlyA) of Escherichia coli. Binding of the MAbs to native, denatured, and erythrocyte-complexed active wild-type hemolysin and mutant derivatives was tested. All 15 MAbs analyzed bound to native hemolysin, even when the toxin was com...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.5.1846-1852.1991
更新日期:1991-05-01 00:00:00
abstract::Bacterial infection with group B Streptococcus (GBS) represents a prominent threat to neonates and fetuses in the Western world, causing severe organ damage and even death. To improve current therapeutic strategies and to investigate new approaches, an appropriate in vivo model to study the immune response of a human ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01235-12
更新日期:2013-05-01 00:00:00
abstract::Female LSH hamsters infected with Treponema pallidum subsp, endemicum before pregnancy or during early pregnancy transmit a form of syphilis to the fetus that is similar to human congenital syphilis. The offspring develops rhinitis, skin rash, failure to thrive, and hepatosplenomegaly. T. pallidum is detectable in the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.8.3559-3561.1993
更新日期:1993-08-01 00:00:00
abstract::Citrobacter rodentium, a murine model pathogen for enteropathogenic Escherichia coli, colonizes the surface of intestinal epithelial cells and causes mucosal inflammation. This bacterium is an ideal model for investigating pathogen-host immune interactions in the gut. It is well known that gene transcripts for Th1 cyt...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01343-09
更新日期:2010-06-01 00:00:00
abstract::We have investigated the possibility that nitric oxide (NO) synthesis may affect the course of a trypanosome infection via T-cell responses using mice deficient in inducible NO synthase (iNOS). Parasitemia levels increased at the same rate in both iNOS-deficient homozygous and control heterozygous mice, and peak paras...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.7.3334-3338.1999
更新日期:1999-07-01 00:00:00
abstract::Tritrichomonas foetus is a serious veterinary pathogen, causing bovine trichomoniasis, a sexually transmitted disease leading to infertility and abortion. T. foetus infects the mucosal surfaces of the reproductive tract. Infection with T. foetus leads to apoptotic cell death of bovine vaginal epithelial cells (BVECs) ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.7.4151-4158.2004
更新日期:2004-07-01 00:00:00
abstract::Recent reports have suggested that oral vaccination of mice against Helicobacter pylori is dependent on a Th1-mediated immune response. However, oral vaccination in mice neither induces sterilizing immunity nor leads to complete protection from disease. Therefore, in this study we investigated whether a systemic subcu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.2.1029-1035.2004
更新日期:2004-02-01 00:00:00
abstract::Previous attempts to induce mucosal antibodies in rhesus monkeys by enteric immunization have resulted in only modest and short-lived responses, dominated by immunoglobulin M (IgM) antibodies in the plasma. In this study, two groups of rhesus monkeys were immunized intranasally three times at 2-week intervals with a b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.4.1272-1283.1996
更新日期:1996-04-01 00:00:00
abstract::We attempted to induce experimental arthritis in rats by systematically testing the effect of Yersinia infections in five strains of rats, using the intragastric, intraperitoneal, and intravenous routes of inoculation. We observed that Lewis rats which were given 10(4) to 10(5) Yersinia enterocolitica WA organisms via...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.3.721-726.1987
更新日期:1987-03-01 00:00:00
abstract::Toll-like receptor (TLR)-based signaling pathways in the host may be modulated by pathogens during the course of infection. We describe a novel immunomodulatory mechanism in which Aspergillus fumigatus conidia induce attenuation of TLR2- and TLR4-mediated interleukin (IL)-6 and IL-1beta proinflammatory responses in hu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01455-08
更新日期:2009-05-01 00:00:00
abstract::Susceptibility of Syrian hamsters of the inbred LSH and MHA strains to injection of as few as 10 plaque-forming units of vesicular stomatitis virus (VSV) was shown to occur only after intraperitoneal and intrapleural injection and not after injection of VSV intravenously, intranasally, or in the footpads. Despite the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.2.540-549.1981
更新日期:1981-11-01 00:00:00