Abstract:
:CD14, the leukocyte receptor for lipopolysaccharide (LPS), is important in the response of human polymorphonuclear leukocytes (PMNs) to infection with gram-negative bacteria. The level of CD14 on the PMN surface increases after exposure to some inflammatory stimuli such as N-formyl-methionyl-leucyl-phenylalanine (fMLP). These newly expressed CD14 molecules probably come from an intracellular pool of preformed receptors. We sought to further characterize PMN CD14 expression, upregulation, and shedding and to define the intracellular location of CD14 molecules. Our results demonstrate that both LPS and fMLP significantly increased CD14 cell surface expression; however, neither phorbol myristate acetate (PMA) or A23187 increased receptor levels on the PMN surface. Neither fMLP, PMA, or A23187 stimulated the release of soluble CD14 from PMNs. Intracellular CD14 was observed in >90% of PMNs examined by flow cytometry and confocal microscopy. Additional analyses using CD14 enzyme-linked immunosorbent assays and electron microscopy studies, examining PMN granules separated by discontinuous sucrose or Percoll gradients, showed that CD14 was present in both the plasma membrane-secretory vesicle fractions and azurophilic granules.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Rodeberg DA,Morris RE,Babcock GFdoi
10.1128/IAI.65.11.4747-4753.1997subject
Has Abstractpub_date
1997-11-01 00:00:00pages
4747-53issue
11eissn
0019-9567issn
1098-5522journal_volume
65pub_type
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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pub_type: 杂志文章
doi:10.1128/IAI.60.9.3814-3823.1992
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doi:10.1128/IAI.45.1.293-296.1984
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
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