Abstract:
:Escherichia coli hemolysin is considered an important virulence factor in extraintestinal E. coli infections. The present study demonstrates that cultured pulmonary artery endothelial cells are susceptible to attack by low concentrations of E. coli hemolysin (greater than or equal to 0.05 hemolytic units/ml; greater than or equal to 5 ng/ml). Sublytic amounts of hemolysin increased the permeability of endothelial cell monolayers in a time- and dose-dependent manner. The hydraulic conductivity increased approximately 30-fold and the reflection coefficient for large molecules dropped from 0.71 to less than 0.05, indicating a toxin-induced loss of endothelial barrier function. The alterations of endothelial monolayer permeability were accompanied by cell retraction and interendothelial gap formation. In addition, E. coli hemolysin stimulated prostacyclin synthesis in endothelial cells. This effect was strictly dependent on the presence of extracellular Ca2+ but not of Mg2+. An enhanced passive influx of 45Ca2+ and 3H-sucrose but not of tritiated inulin and dextran was noted in toxin-treated cells, indicating that small transmembrane pores comparable to those detected in rabbit erythrocytes had been generated in endothelial cell membranes. These pores may act as nonphysiologic Ca2+ gates, thereby initiating different Ca2+-dependent cellular processes. We conclude that endothelial cells are highly susceptible to E. coli hemolysin and that two major endothelial cell functions are altered by very low concentrations of hemolysin.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Suttorp N,Flöer B,Schnittler H,Seeger W,Bhakdi Sdoi
10.1128/IAI.58.11.3796-3801.1990subject
Has Abstractpub_date
1990-11-01 00:00:00pages
3796-801issue
11eissn
0019-9567issn
1098-5522journal_volume
58pub_type
杂志文章abstract::Three MudJ prototrophs demonstrated that intracellular replication is a Salmonella virulence trait (K. Y. Leung and B. B. Finlay, Proc. Natl. Acad. Sci. USA, 88:11470-11474, 1991). mutS and mutH are disrupted in mutants 3-11 and 12-23, and ssaQ is disrupted in mutant 17-21. Further analysis revealed that loss of Salmo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.4.2247-2252.2003
更新日期:2003-04-01 00:00:00
abstract::D-Amino acid oxidase (DAO) is a hydrogen peroxide-generating enzyme that uses a D-amino acid as a substrate. We hypothesized that DAO may protect against bacterial infection, because hydrogen peroxide is one of the most important molecules in the antibacterial defense systems in mammals. We show here that DAO suppress...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.06214-11
更新日期:2012-04-01 00:00:00
abstract::Filifactor alocis is a recently recognized periodontal pathogen; however, little is known regarding its interactions with the immune system. As the first-responder phagocytic cells, neutrophils are recruited in large numbers to the periodontal pocket, where they play a crucial role in the innate defense of the periodo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00496-16
更新日期:2016-11-18 00:00:00
abstract::Human galectin-3 binds to the surface of Trypanosoma cruzi trypomastigotes and human coronary artery smooth muscle (CASM) cells. CASM cells express galectin-3 on their surface and secrete it. Exogenous galectin-3 increased the binding of T. cruzi to CASM cells. Trypanosome binding to CASM cells was enhanced when eithe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.11.6717-6721.2004
更新日期:2004-11-01 00:00:00
abstract::A defect in lymphocyte function could be responsible for persistent infection by the equine infectious anemia virus. The number of lymphocytes bearing surface immunoglobulin, as detected by immunofluorescence, and lymphocyte response to mitogens were the same in uninfected and equine infectious anemia-infected animals...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.8.4.679-682.1973
更新日期:1973-10-01 00:00:00
abstract::Yersinia pestis causes a rapid, lethal disease referred to as plague. Y. pestis actively inhibits the innate immune system to generate a noninflammatory environment during early stages of infection to promote colonization. The ability of Y. pestis to create this early noninflammatory environment is in part due to the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00909-19
更新日期:2020-02-20 00:00:00
abstract::Both idiopathic and infectious forms of colitis disrupt normal intestinal epithelial cell (IEC) proliferation and differentiation, although the mechanisms involved remain unclear. Recently, we demonstrated that infection by the attaching and effacing murine pathogen Citrobacter rodentium leads to a significant reducti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00655-13
更新日期:2013-12-01 00:00:00
abstract::The concept of translational research, which aims to facilitate the application of basic scientific discoveries in clinical and community settings, is currently in vogue. While there are powerful forces driving this trend, support for translational research must be accompanied by a robust investment in basic science, ...
journal_title:Infection and immunity
pub_type: 社论,评审
doi:10.1128/IAI.01318-09
更新日期:2010-02-01 00:00:00
abstract::Macrophages can undergo apoptosis after infection with Mycobacterium tuberculosis. This macrophage response deprives the bacillus of its niche cell and supports the host response through better antigen presentation. The intracellular pathways of apoptosis that elaborate this macrophage response are not well understood...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01107-06
更新日期:2007-04-01 00:00:00
abstract::We investigated the role of bacterial mannose-resistant fimbriation of S fimbriae (Fim), mannose-resistant hemagglutination (S-Mrh), and hemolysin (Hly) production by an Escherichia coli parent and genetically cloned strains as regards their effect on histamine release from rat mast cells and generation of the chemilu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.3.886-892.1986
更新日期:1986-12-01 00:00:00
abstract::Salmonellae possess the ability to adhere to and invade macrophages and in so doing trigger a number of intracellular events that are associated with cellular activation. As an initial approach to defining the mechanisms by which invasive salmonellae alter macrophage function, we have explored the impact of Salmonella...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.5.1820-1826.1995
更新日期:1995-05-01 00:00:00
abstract::Exoantigens of Plasmodium vivax-parasitized erythrocytes stimulated macrophages to secrete tumor necrosis factor, and antisera raised against the exoantigens inhibited this secretion. The antisera also inhibited the activity of Plasmodium falciparum and Plasmodium yoelii exoantigens, and conversely, antisera against t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.3.1241-1243.1992
更新日期:1992-03-01 00:00:00
abstract::A mouse lacking CD28, a T-cell costimulatory molecule, and STAT6, a transcription factor that mediates interleukin-4 (IL-4) signaling, was developed from parental CD28- and STAT6-deficient mice. STAT6/CD28(-/-) BALB/c mice that were 8 weeks old had a normal phenotype, and IL-4 production was induced following infectio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.7.3706-3715.2004
更新日期:2004-07-01 00:00:00
abstract::The Corynebacterium diphtheriae hmuO gene encodes a heme oxygenase that is involved in the utilization of heme as an iron source. Transcription of hmuO is activated by heme or hemoglobin and repressed by iron and DtxR. Previous studies with Escherichia coli showed that heme-dependent transcriptional activation of an h...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.11.7406-7412.2005
更新日期:2005-11-01 00:00:00
abstract::Immunoglobulin G (IgG)- and IgM-specific antibody titers against Salmonella typhi Ty2 porins have been measured in 30 paired typhoid sera by enzyme-linked immunosorbent assay. These studies have found that IgG serum titers of acute and convalescent sera were 625 and 5,000 times higher, respectively than the control se...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.52.1.209-212.1986
更新日期:1986-04-01 00:00:00
abstract::The parameters of a persistent-state measles virus infection in BGM/MV cells were examined. The BGM/MV cell line was established by cocultivation of measles virus-infected primary C3H mouse brain cells with a stable line of African green monkey kidney cells (BGM). Initially, a morphologically mixed population of cells...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.1.152-158.1975
更新日期:1975-01-01 00:00:00
abstract::Protective immunity against Babesia bigemina is hypothesized to involve antibodies directed against merozoite surface-exposed epitopes. Levels of antibody against a rhoptry-associated protein 1 (RAP-1) B-lymphocyte epitope, defined by surface-reactive and inhibitory monoclonal antibodies, in immune cattle sera were de...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.12.5698-5701.1994
更新日期:1994-12-01 00:00:00
abstract::Acute lethality was induced in rabbits by the sequential injection of Propionibacterium acnes and lipopolysaccharide (LPS). P. acnes induced the infiltration of inflammatory cells into the liver lobules during the early phase, and LPS in the late phase caused death in association with pathological changes mimicking he...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.12.4812-4817.1995
更新日期:1995-12-01 00:00:00
abstract::The ultrastructural appearance of the "halozone" formed at the interface between the spotted fever agent Rickettsia rickettsii and the cytoplasm of persistently infected cultured vole cells (Microtus pennsylvanicus) was studied by transmission electron microscopy. In sections of epoxy-embedded specimens stained with u...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.41.3.1252-1260.1983
更新日期:1983-09-01 00:00:00
abstract::The interaction of the first component of complement with two serum-sensitive strains of Escherichia coli and Klebsiella pneumoniae was studied. It could be demonstrated that highly purified C1, free of immunoglobulin G and immunoglobulin M, binds to E. coli or K. pneumoniae. C1 binding was also found with specificall...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.22.1.5-9.1978
更新日期:1978-10-01 00:00:00
abstract::Mucosa-associated invariant T (MAIT) cells are an innate-like T cell subset in mammals that recognize microbial vitamin B metabolites presented by the evolutionarily conserved major histocompatibility complex class I (MHC I)-related molecule, MR1. Emerging data suggest that MAIT cells may be an attractive target for v...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00524-20
更新日期:2020-12-15 00:00:00
abstract::Pseudomonas aeruginosa exotoxin A (PA), a potent protein synthesis inhibitor, was found to be a weak T-cell mitogen for murine splenocytes. Maximal stimulation of [3H]thymidine incorporation was obtained with 10 to 100 ng of toxin per ml following a 4-day induction. PA was also shown to be a polyclonal activator of cy...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.1.213-218.1988
更新日期:1988-01-01 00:00:00
abstract::Proteus mirabilis, a common agent of nosocomially acquired and catheter-associated urinary tract infection, is the most frequent cause of infection-induced bladder and kidney stones. Urease-catalyzed urea hydrolysis initiates stone formation in urine and can be inhibited by acetohydroxamic acid and other structural an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.10.3360-3365.1991
更新日期:1991-10-01 00:00:00
abstract::In studies of immunity to malaria, the absence of febrile malaria is commonly considered evidence of "protection." However, apparent "protection" may be due to a lack of exposure to infective mosquito bites or due to immunity. We studied a cohort that was given curative antimalarials before monitoring began and docume...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01358-08
更新日期:2009-05-01 00:00:00
abstract::Evidence obtained from gene knockout studies supports the role of Toll-like receptor 4 (TLR4) in intestinal inflammation and microbiota recognition. Increased epithelial TLR4 expression is observed in patients with inflammatory bowel disease. However, little is known of the effect of increased TLR4 signaling on intest...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01374-15
更新日期:2016-01-11 00:00:00
abstract::Placenta-sequestering Plasmodium falciparum involved in the pathogenesis of pregnancy-associated malaria (PAM) in otherwise clinically immune women expresses particular variant surface antigens (VSA(PAM)) on the surface of infected erythrocytes that differ from VSA found in parasitized nonpregnant individuals (non-PAM...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.7.4112-4118.2005
更新日期:2005-07-01 00:00:00
abstract::Monoclonal antibodies to the capsular polysaccharide of Cryptococcus neoformans produce distinct capsular reactions and have biological activities that are determined by serotype specificity. In the present study, polyclonal rabbit anticapsular antibodies were cross-absorbed to produce serotype specificities similar t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.2.1189-1191.2001
更新日期:2001-02-01 00:00:00
abstract::γ9δ2 T cells provide a natural bridge between innate and adaptive immunity, rapidly and potently respond to pathogen infection in mucosal tissues, and are prominently induced by both tuberculosis (TB) infection and bacillus Calmette Guérin (BCG) vaccination. Mycobacterium-expanded γ9δ2 T cells represent only a subset ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01322-15
更新日期:2016-08-19 00:00:00
abstract::The genome of Borrelia burgdorferi, the etiologic agent of Lyme disease, is composed of a linear chromosome and more than 20 linear and circular plasmids. Typically, plasmid content analysis has been carried out by pulsed-field gel electrophoresis and confirmed by Southern hybridization. However, multiple plasmids of ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.7.3699-3706.2003
更新日期:2003-07-01 00:00:00
abstract::Infection with the intracellular protozoan parasite Toxoplasma gondii causes serious public health problems and is of great economic importance worldwide. The micronemal protein MIC3, which is a potent adhesin of T. gondii, could be a significant candidate vaccine against toxoplasmosis. In this study, all CBA/J mice i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.11.6222-6228.2003
更新日期:2003-11-01 00:00:00