Abstract:
:Gnotobiotic calves were inoculated with an O5:K4:H-, urease-positive strain of Escherichia coli isolated from a 2-day-old calf with diarrhea. The calves developed elevated temperatures and passed loose mucoid feces, with or without blood. The E. coli strain was negative for heat-stable and heat-labile enterotoxins but produced high levels of Shiga-like toxin. Bacteria attached diffusely to the epithelium of the large intestine and multifocally to the epithelium of the ileum. The duodenum and jejunum were not affected. At the sites of bacterial attachment, microvilli were effaced, enterocytes were degenerate, and necrosis and exfoliation had occurred. These results confirm a previous report from England that calves may naturally contract infections similar to those caused by enteropathogenic E. coli strains pathogenic to humans or rabbits. This suggests that the calf bacterial strains, like some enteropathogenic E. coli strains, produce high levels of Shiga-like toxin and cause attachment and effacement lesions in the colonic epithelium of the infected host.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Moxley RA,Francis DHdoi
10.1128/IAI.53.2.339-346.1986subject
Has Abstractpub_date
1986-08-01 00:00:00pages
339-46issue
2eissn
0019-9567issn
1098-5522journal_volume
53pub_type
杂志文章abstract::A complex of trehalose-6, 6'-dimycolate (cord factor) and methylated bovine serum albumin was prepared, and its effect on the toxicity of cord factor in mice was studied. Either the active immunization with cord factor-methylated bovine serum albumin complex or the passive transfer of sera of rabbits vaccinated with t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.5.2.203-212.1972
更新日期:1972-02-01 00:00:00
abstract::Aspergillus species are saprophytic molds causing life-threatening invasive fungal infections in the immunocompromised host. Innate immune recognition, in particular, the mechanisms of opsonization and complement activation, has been reported to be an integral part of the defense against fungi. We have shown that the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00032-13
更新日期:2013-05-01 00:00:00
abstract::Tuberculous pleurisy allows the study of human cells at the site of active Mycobacterium tuberculosis infection. In this study, we found that among pleural fluid (PF) lymphocytes, natural killer (NK) cells are a major source of early gamma interferon (IFN-gamma) upon M. tuberculosis stimulation, leading us to investig...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00381-07
更新日期:2007-11-01 00:00:00
abstract::Murine embryo fibroblasts (MEF) were found to secrete tumor necrosis factor (TNF) in response to stimulation with endotoxin. Endotoxin-induced TNF production by MEF was inhibited by cycloheximide. However, reversal of the effect of this inhibitor on protein synthesis results in TNF being secreted in amounts equivalent...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.5.1630-1635.1993
更新日期:1993-05-01 00:00:00
abstract::A secreted nuclease, SsnA, was identified in the virulent Streptococcus suis isolate SX332 and subsequently in each of the type strains of capsular serotypes 1 through 9. Screening of 258 porcine clinical isolates from surface (nasal mucosa or palatine tonsil) or internal (joint, brain or other internal organ) locatio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.2.774-781.2004
更新日期:2004-02-01 00:00:00
abstract::The leading malaria vaccine candidate, RTS,S, based on the Plasmodium falciparum circumsporozoite protein (CSP), will likely be the first publicly adopted malaria vaccine. However, this and other subunit vaccines, such as virus-vectored thrombospondin-related adhesive protein (TRAP), provide only intermediate to low l...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01063-15
更新日期:2015-12-14 00:00:00
abstract::We have examined the ability of peptidoglycan (PepG) and lipoteichoic acid (LTA) isolated from Staphylococcus aureus to induce the release of tumor necrosis factor alpha (TNF-alpha), interleukin-6 (IL-6), and IL-10 in whole human blood and identified the cellular origins of these cytokines. Both PepG and LTA induced t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.7.3965-3970.2000
更新日期:2000-07-01 00:00:00
abstract::Secretory immunoglobulin A (SIgA) antibodies directed against the O antigen of lipopolysaccharide (LPS) are the primary determinants of mucosal immunity to gram-negative enteric pathogens. However, the underlying mechanisms by which these antibodies interfere with bacterial colonization and invasion of intestinal epit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00416-08
更新日期:2008-09-01 00:00:00
abstract::The enzymes responsible for reducing H2O2 were surveyed in 49 strains of Leptospira by using semiquantitative assays for catalase and peroxidase. The survey revealed a differential distribution of catalase and peroxidase activities between the two leptospiral complexes. The pathogenic Leptospira interrogans strains ga...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.22.3.672-675.1978
更新日期:1978-12-01 00:00:00
abstract::We have investigated the possibility that nitric oxide (NO) synthesis may affect the course of a trypanosome infection via T-cell responses using mice deficient in inducible NO synthase (iNOS). Parasitemia levels increased at the same rate in both iNOS-deficient homozygous and control heterozygous mice, and peak paras...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.7.3334-3338.1999
更新日期:1999-07-01 00:00:00
abstract::The mechanism of enhanced resistance of Mycobacterium bovis BCG-treated mice to ectromelia virus infection was investigated by determining the effect of splenectomy, antithymocyte serum, and antimacrophage serum on resistance. It was greatly reduced by these treatments, not only in normal mice, but also in mice treate...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.2.567-573.1983
更新日期:1983-11-01 00:00:00
abstract::The invasion of Rickettsia tsutsugamushi, Gilliam strain, into guinea pig polymorphonuclear leukocytes (PMNs) and the localization and distribution of tracers were followed during the process by electron microscopy. The seven tracers used were: cationized ferritin, ferritin, thorium dioxide (ThO2), carbon particles, l...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.30.1.231-243.1980
更新日期:1980-10-01 00:00:00
abstract::NF-κB is a critical regulator of gene expression during severe infections. NF-κB comprises homo- and heterodimers of proteins from the Rel family. Among them, p50 and p65 have been clearly implicated in the pathophysiology of sepsis. In contrast, the role of cRel in sepsis is still controversial and has been poorly st...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00021-11
更新日期:2011-05-01 00:00:00
abstract::An inactivated oral enterotoxigenic Escherichia coli (ETEC) vaccine against ETEC diarrhea was given to 25 adult Swedish volunteers. The vaccine consisted of formalin-killed E. coli bacteria expressing the most common colonization factor antigens (CFAs), i.e., CFA/I, -II, and -IV, and recombinantly produced cholera B s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.7.3311-3316.1998
更新日期:1998-07-01 00:00:00
abstract::Mannose-binding lectin (MBL) is an important component of the innate immune system. It binds to the arrays of sugars commonly presented by microorganisms and activates the complement system independently of antibody. Despite detailed knowledge of the stereochemical basis of MBL binding, relatively little is known abou...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.7.3894-3899.2000
更新日期:2000-07-01 00:00:00
abstract::The comparative immunogenicity of the two cross-reacting group 6 pneumococcal capsular polysaccharides, type 6A(6) and type 6B(26), was studied with hyperimmune rabbit typing antisera and with sera from adult volunteers injected with polyvalent pneumococcal vaccines containing either 50 mug of type 6A (U.S. designatio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.3.1116-1122.1979
更新日期:1979-12-01 00:00:00
abstract::The lymphogranuloma venereum (LGV) and trachoma biovars of Chlamydia trachomatis exhibit differences in biological properties both in vivo and in vitro. To identify analogous biochemical differences, we studied the molecular charges of chlamydial outer membrane proteins (OMPs) by means of isoelectric focusing and none...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.2.488-494.1985
更新日期:1985-11-01 00:00:00
abstract::Brucella organisms are intracellular stealth pathogens of animals and humans. The bacteria overcome the assault of innate immunity at early stages of an infection. Removal of polymorphonuclear neutrophils (PMNs) at the onset of adaptive immunity against Brucella abortus favored bacterial elimination in mice. This was ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00118-19
更新日期:2019-04-23 00:00:00
abstract::The effects of tolerance to Escherichia coli endotoxin on the phagocytic and bactericidal activity of the hepatic reticuloendothelial system against viable E. coli were examined using ex vivo perfused rat livers. Livers were isolated from control and endotoxin-tolerant rats and perfused with a medium containing 5% hom...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.3.798-803.1980
更新日期:1980-03-01 00:00:00
abstract::The method of hemagglutination inhibition was used to investigate the antigenic diversity of lipopolysaccharide (LPS) from Neisseria meningitidis and to develop a serotyping systems based on this antigen. The system uses outer membrane complex prepared by a simple extraction procedure to inhibit homologous hemagglutin...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.2.471-475.1977
更新日期:1977-05-01 00:00:00
abstract::Anaplasma marginale subsp. centrale was the first vaccine used to protect against a rickettsial disease and is still in widespread use a century later. As its use preceded development of either cryopreservation or cell culture, the vaccine strain was maintained for decades by sequential passage among donor animals, ex...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00199-13
更新日期:2013-05-01 00:00:00
abstract::We report on the identification of a surface-exposed, highly conserved, immunogenic nontypeable Haemophilus influenzae (NTHi) protein, which elicits cross-reactive bactericidal antibodies against NTHi. The protein was extracted from NTHi strain P860295 with KSCN and purified; it migrated as a single band on a sodium d...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.5.2525-2534.2000
更新日期:2000-05-01 00:00:00
abstract::Endogenous interleukin-12 (IL-12) mediates protection against Yersinia enterocolitica in C57BL/6 mice by triggering gamma interferon (IFN-gamma) production in NK and CD4+ T cells. Administration of exogenous IL-12 confers protection against yersiniae in Yersinia-susceptible BALB/c mice but exacerbates yersiniosis in r...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.5.2213-2220.1998
更新日期:1998-05-01 00:00:00
abstract::We have isolated three genes, ARG57, SER57, and LYS1, on the basis of their function in Candida albicans. A C. albicans transformation vector containing the C. albicans URA3 gene, a Candida ARS sequence, and a portion of the Saccharomyces cerevisiae 2 microns circle containing the replication origin was constructed. C...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.3.876-884.1992
更新日期:1992-03-01 00:00:00
abstract::Streptococcus pneumoniae contains an inhibitor of human neutrophil elastase. The agent does not inhibit other proteases, including neutrophil cathepsin G and pancreatic elastase. It is active in the presence of insoluble elastin as well as synthetic elastase substrates. The inhibitor is present in the pneumococcal cel...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.49.1.52-60.1985
更新日期:1985-07-01 00:00:00
abstract::A rapid and reproducible method of bacteriocin typing is described based on leakage of ultraviolet light-absorbing material (UVAM), detectable in supernatants of bacteriocin-sensitive cultures, by means of a spectrophotometer. The prerequisites for reproducible results, with nonsignificant fluctuations in standard err...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.1.12-19.1977
更新日期:1977-04-01 00:00:00
abstract::Polymicrobial intra-abdominal infections (IAI) are clinically prevalent and cause significant morbidity and mortality, especially those involving fungi. Our laboratory developed a mouse model of polymicrobial IAI and demonstrated that coinfection with Candida albicans and Staphylococcus aureus (C. albicans/S. aureus) ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00348-19
更新日期:2019-07-23 00:00:00
abstract::Salmonella enterica serovar Enteritidis is a major cause of food-borne diseases associated with consumption of shell eggs. Clinical isolates of S. enterica serovar Enteritidis exhibit a wide spectrum of virulence in mice. A highly virulent isolate (SE2472) was previously shown to be more resistant in vitro than other ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.2.451-461.2002
更新日期:2002-02-01 00:00:00
abstract::Lactoferrin (Lf) is an iron-binding antimicrobial protein present in milk and on mucosal surfaces, with a suggested role in preimmune host defense. Certain strains of Escherichia coli (bacterial whole cells) demonstrate specific interaction with 125I-labeled Lf. A band with a mass of approximately 37 kDa, which was re...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.4.1236-1240.1994
更新日期:1994-04-01 00:00:00
abstract::The concept that relates low virulence of certain alphaviruses to low viremia and efficient vascular clearance of virus was tested in guinea pigs. Previously published studies with hamsters suggested that virulent strains maintain high viremias primarily because they are cleared inefficiently from the blood. In the pr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.17.2.356-360.1977
更新日期:1977-08-01 00:00:00