Abstract:
:Several bacterial species recruit the complement regulators C4b-binding protein, factor H, and vitronectin, resulting in resistance against the bactericidal activity of human serum. It was recently demonstrated that bacteria also bind plasminogen, which is converted to plasmin that degrades C3b and C5. In this study, we found that a series of clinical isolates (n = 58) of the respiratory pathogen Moraxella catarrhalis, which is commonly isolated from preschool children and adults with chronic obstructive pulmonary disease (COPD), significantly binds human plasminogen. Ubiquitous surface protein A2 (UspA2) and hybrid UspA2 (UspA2H) were identified as the plasminogen-binding factors in the outer membrane proteome of Moraxella. Furthermore, expression of a series of truncated recombinant UspA2 and UspA2H proteins followed by a detailed analysis of protein-protein interactions suggested that the N-terminal head domains bound to the kringle domains of plasminogen. The binding affinity constant (KD) values of full-length UspA2(30-539) (amino acids 30 to 539 of UspA2) and full-length UspA2H(50-720) for immobilized plasminogen were 4.8 × 10(-8) M and 3.13 × 10(-8) M, respectively, as measured by biolayer interferometry. Plasminogen bound to intact M. catarrhalis or to recombinant UspA2/UspA2H was readily accessible for a urokinase plasminogen activator that converted the zymogen into active plasmin, as verified by the specific substrate S-2251 and a degradation assay with fibrinogen. Importantly, plasmin bound at the bacterial surface also degraded C3b and C5, which consequently may contribute to reduced bacterial killing. Our findings suggest that binding of plasminogen to M. catarrhalis may lead to increased virulence and, hence, more efficient colonization of the host.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Singh B,Al-Jubair T,Voraganti C,Andersson T,Mukherjee O,Su YC,Zipfel P,Riesbeck Kdoi
10.1128/IAI.00310-15subject
Has Abstractpub_date
2015-09-01 00:00:00pages
3458-69issue
9eissn
0019-9567issn
1098-5522pii
IAI.00310-15journal_volume
83pub_type
杂志文章abstract::In an earlier report, we had described the isolation and characterization of autolysis-defective mutants of Staphylococcus aureus (N. Mani, P. Tobin, and R.K. Jayaswal, J. Bacteriol. 175:1493-1499, 1993). In the study reported here, an autolysis-defective mutant showed attenuated virulence in a rat model of experiment...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.4.1406-1409.1994
更新日期:1994-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.9.3139-3142.1990
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.4.942-946.1987
更新日期:1987-04-01 00:00:00
abstract::Clostridium difficile toxins A and B (TcdA and TcdB) are the causative agents of antibiotic-associated pseudomembranous colitis. Mucosal mast cells play a crucial role in the inflammatory processes underlying this disease. We studied the direct effects of TcdA and TcdB on the human mast cell line HMC-1 with respect to...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00195-07
更新日期:2007-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.10.4075-4081.1997
更新日期:1997-10-01 00:00:00
abstract::Oligosaccharide (OS)-protein conjugates are promising candidate vaccines against encapsulated bacteria, such as Haemophilus influenzae, Neisseria meningitidis, and Streptococcus pneumoniae. Although the effects of several variables such as OS chain length and protein carrier have been studied, little is known about th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.12.6915-6920.2003
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.51.1.250-253.1986
更新日期:1986-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.10.2327-2331.1987
更新日期:1987-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.1.142-145.1985
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.10.4222-4228.1997
更新日期:1997-10-01 00:00:00
abstract::The parameters of a persistent-state measles virus infection in BGM/MV cells were examined. The BGM/MV cell line was established by cocultivation of measles virus-infected primary C3H mouse brain cells with a stable line of African green monkey kidney cells (BGM). Initially, a morphologically mixed population of cells...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.1.152-158.1975
更新日期:1975-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00711-15
更新日期:2015-12-07 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.6.1991-1996.1991
更新日期:1991-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.2.874-877.1998
更新日期:1998-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.7.4329-4336.2001
更新日期:2001-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.53.1.213-220.1986
更新日期:1986-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01322-15
更新日期:2016-08-19 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.1.262-264.1986
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journal_title:Infection and immunity
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doi:10.1128/IAI.55.10.2509-2517.1987
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.5.1003-1009.1974
更新日期:1974-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2009-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.1.116-121.1997
更新日期:1997-01-01 00:00:00
abstract::Rabbits are not usually susceptible to intestinal Shigella infection without extensive pretreatment, including starvation and administration of antimicrobial, antimotility, and toxic agents (carbon tetrachloride). Most animals infected this way die rapidly and do not always develop colonic lesions and signs of dysente...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.11.4350-4357.1995
更新日期:1995-11-01 00:00:00
abstract::Macrophage-dependent killing of facultative intracellular bacteria was markedly impaired by overt erythrocytic Plasmodium yoelii or Plasmodium berghei infection of mice. P. yoelii infection was capable of ablating not only the macrophage microbicidal capacity of "normal" animals but also the bactericidal capacities of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.1.396-407.1981
更新日期:1981-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.9.3724-3729.1993
更新日期:1993-09-01 00:00:00
abstract::NF-κB is a critical regulator of gene expression during severe infections. NF-κB comprises homo- and heterodimers of proteins from the Rel family. Among them, p50 and p65 have been clearly implicated in the pathophysiology of sepsis. In contrast, the role of cRel in sepsis is still controversial and has been poorly st...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00021-11
更新日期:2011-05-01 00:00:00
abstract::The mechanisms of genetic resistance or "trypanotolerance" to infection with Trypanosoma congolense were investigated in two strains of mice. One strain C57BL, is outstandingly resistant to most stabilates of T. congolense and can survive for over 80 days, whereas CFLP, in common with most other strains, generally suc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.3.707-713.1980
更新日期:1980-03-01 00:00:00
abstract::Polyinosinate-polycytidylate [poly(I).poly(C)]-induced interferon production in cultures of human foreskin fibroblast strains was increased by ultraviolet irradiation of cells at the time of exposure to inducer or at 2 h after induction. Incubation of cells with interferon prior to induction (priming) and ultraviolet ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.5.1189-1191.1974
更新日期:1974-11-01 00:00:00