Abstract:
:To study responses to Mycobacterium leprae antigens, we developed an in vitro model system in which latex particles coated with M. leprae sonic extract (MLSON) antigen were presented to monocytes. Uptake and oxidative response as measured by superoxide production to these antigens were investigated. Phagocytosis of MLSON-coated particles was greater than that of control particles in monocytes from both leprosy patients and controls from leprosy-endemic areas; uptake of MLSON-coated particles was higher in monocytes from lepromatous leprosy patients than in cells from tuberculoid leprosy patients and controls. In both patients and controls, uptake of latex particles coated with leprosy antigens triggered very little reduction of nitroblue tetrazolium although the cells were capable of mounting a respiratory burst. Antigen-coated latex particles can therefore be used as a tool to investigate monocyte responses to M. leprae and individual recombinant antigens.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Hasan RS,Dockrell HM,Jamil S,Chiang TJ,Hussain Rdoi
10.1128/IAI.61.9.3724-3729.1993subject
Has Abstractpub_date
1993-09-01 00:00:00pages
3724-9issue
9eissn
0019-9567issn
1098-5522journal_volume
61pub_type
杂志文章abstract::Streptococcus mutans, a major pathogen of dental caries, is considered one of the causative agents of infective endocarditis (IE). Recently, bacterial DNA encoding 120-kDa cell surface collagen-binding proteins (CBPs) has frequently been detected from S. mutans-positive IE patients. In addition, some of the CBP-positi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00401-17
更新日期:2017-11-17 00:00:00
abstract::Conditions for the release of streptococcal type 12 M protein from whole cells by cyanogen bromide are described; they demonstrated that methionine is not essential to the structural arrangements which account for some of its immunological and biological properties. The released M protein was separated from other prot...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.2.546-555.1978
更新日期:1978-08-01 00:00:00
abstract::A differential-display PCR procedure identified the capsular assembly gene kpsD after Escherichia coli type 1 fimbrial binding to mannose-coated Sepharose beads. Limiting-dilution reverse-transcribed PCRs confirmed down-regulation of the kpsD gene, and Northern blot and lacZ fusion analyses showed down-regulation of t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.2.1226-1231.2005
更新日期:2005-02-01 00:00:00
abstract::The population structure of the Bacillus cereus group (52 strains of B. anthracis, B. cereus, and B. thuringiensis) was investigated by sequencing seven gene fragments (rpoB, gyrB, pycA, mdh, mbl, mutS, and plcR). Most of the strains were classifiable into two large subgroups in six housekeeping gene trees but not in ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.9.5253-5261.2004
更新日期:2004-09-01 00:00:00
abstract::Bordetella pertussis adenylate cyclase-hemolysin (AC-Hly), encoded by the cyaA gene, belongs to the RTX family of toxins with extensive glycine-rich repeats in the carboxy-terminal portion. AC-Hly possesses both adenylate cyclase toxic and hemolytic activities that depend on a posttranslational modification mediated b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.9.3583-3589.1993
更新日期:1993-09-01 00:00:00
abstract::Staphylococcus aureus is a pathogen that causes significant morbidity and mortality. Nasal carriage is a major source of transmission and of endogenous infection. Persistent carriage is detected in ∼30% of healthy individuals. While Th17 cells have been shown to play a role in S. aureus infection and clearance, the im...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00834-17
更新日期:2018-02-20 00:00:00
abstract::Cell wall lytic enzyme (Kyowa lytic no. 2 enzyme) liberated arthritogenic hydrosoluble peptidoglycans from both arthritogenic and non-arthritogenic bacterial cell walls. From these cell walls, mutanolysin (peptidoglycan-degrading enzyme) also liberated hydrosoluble peptidoglycans which, however, lacked arthritogenicit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.3.861-866.1977
更新日期:1977-06-01 00:00:00
abstract::The purpose of the present study was to determine the extent of immunologic responses, particularly immunopathologic responses, within the upper and lower respiratory tracts after intranasal immunization using the mucosal adjuvant cholera toxin (CT). BALB/c mice were nasally immunized with influenza virus vaccine comb...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.4.2328-2338.2001
更新日期:2001-04-01 00:00:00
abstract::An immunogenic aminopeptidase was purified from Brucella melitensis strain VTRM1. The purification procedure consisted of ammonium sulfate fractionation and three chromatographic steps. This procedure resulted in a yield of 29% and a 144-fold increase in specific activity. The aminopeptidase appeared to be a monomeric...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.9.5238-5244.2003
更新日期:2003-09-01 00:00:00
abstract::Salmonella strains that lack or overproduce DNA adenine methylase (Dam) elicit a protective immune response to different Salmonella species. To generate vaccines against other bacterial pathogens, the dam genes of Yersinia pseudotuberculosis and Vibrio cholerae were disrupted but found to be essential for viability. O...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.12.7610-7615.2001
更新日期:2001-12-01 00:00:00
abstract::The effects of heat and chemical inactivation on the antigenicity and immunogenicity of Vibrio cholerae 1418 in rabbits were studied. V. cholerae 1418 was inactivated with heat and chemical inactivants (phenol or Formalin) alone or in combination. Enzyme-linked immunoassay systems employing whole cells of V. cholerae ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.1.21-26.1982
更新日期:1982-10-01 00:00:00
abstract::Trypanosoma brucei subsp. brucei and T. congolense incubated in homologous antibody at 0 degrees C for 30 min were lysed by subsequent addition of guinea pig complement. Trypanosomes incubated in antibody at 37 degrees C before complement treatment remained intact. Parasites bearing adsorbed antibody also remained int...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.3.617-627.1979
更新日期:1979-06-01 00:00:00
abstract::Female guinea pigs were immunized with viable or UV light-inactivated chlamydiae (agent of guinea pig inclusion conjunctivitis), belonging to the species Chlamydia psittaci, by intravenous, subcutaneous, oral, or ocular routes. All animals were then inoculated vaginally with viable chlamydiae to determine the extent o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.8.2599-2605.1990
更新日期:1990-08-01 00:00:00
abstract::A 2.9-kilobase pair locus in Neisseria meningitidis was identified as containing transcriptionally linked open reading frames encoding TolC- and HlyD-like proteins. Although the meningococcal TolC protein was required for extracellular production of the repeats-in-toxin (RTX) FrpC toxin, it could not functionally repl...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01995-06
更新日期:2007-12-01 00:00:00
abstract::The role of the alternative sigma factor sigma(B) in Staphylococcus epidermidis was investigated by the construction, complementation, and characterization of a sigB deletion mutant. Electrophoretic analyses confirmed a profound influence of sigma(B) on the expression of exoproteins and cytoplasmic proteins. Detailed ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.12.7933-7936.2001
更新日期:2001-12-01 00:00:00
abstract::Neospora caninum is a recently identified coccidian parasite which shares many features with, but is clearly distinct from, Toxoplasma gondii. N. caninum tachyzoites infect a wide range of mammalian cells both in vivo and in vitro. The mechanisms by which infection is achieved are largely unknown. Recent evidence has ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.10.4279-4287.1996
更新日期:1996-10-01 00:00:00
abstract::During the course of infection of rabbits with vaccinia virus, macrophages obtained from the peritoneal cavity develop bactericidal activity and the replication of vaccinia virus becomes restricted in these cells. The abortive replication of vaccinia virus in the activated macrophages was characterized in the present ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.1.328-338.1979
更新日期:1979-10-01 00:00:00
abstract::A virus-specific cell surface antigen(s) was detected on mouse adenovirus-infected cells by the immunofluorescent antibody technique. It was demonstrated in infected cells regardless of the mouse strain or animal species used. It became detectable on the cell surface about 6 h postinfection and was demonstrated in 90%...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.18.1.41-45.1977
更新日期:1977-10-01 00:00:00
abstract::Borrelia burgdorferi synthesizes a variety of differentially regulated outer surface lipoproteins in the tick vector and in vertebrate hosts. Among these is OspD, a protein that is highly induced in vitro by conditions that mimic the tick environment. Using genetically engineered strains in which ospD is deleted, we d...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00714-07
更新日期:2008-05-01 00:00:00
abstract::Human sera were examined by an enzyme-linked immunosorbent assay for immunoglobulin G (IgG) and IgM antibodies to purified type III polysaccharide of group B streptococci. The antigen-binding capacity of a reference human serum was determined by a radioimmunoassay, and the total antibody content was determined by quan...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.46.1.98-104.1984
更新日期:1984-10-01 00:00:00
abstract::Gamma interferon (IFN-γ) induces expression of the tryptophan-catabolizing enzyme indoleamine 2,3-dioxygenase (IDO1) in human epithelial cells, the permissive cells for the obligate intracellular bacterium Chlamydia trachomatis. IDO1 depletes tryptophan by catabolizing it to kynurenine with consequences for C. trachom...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05659-11
更新日期:2011-11-01 00:00:00
abstract::Burkholderia cenocepacia is an opportunistic pathogen that primarily infects cystic fibrosis (CF) patients. Previously, we reported that ShvR, a LysR regulator, influences colony morphology, virulence, and biofilm formation and regulates the expression of an adjacent 24-kb genomic region encoding 24 genes. In this stu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00170-11
更新日期:2011-08-01 00:00:00
abstract::Stimulated polymorphonuclear leukocytes have been shown by this laboratory to release a reactive-oxygen species (ROS) which is detectable in the supernatant and is capable of oxidizing reduced glutathione and reacting with methionine (Sagone et al., Blood 63:96-104, 1984). This ROS is dependent on H2O2 and heme for it...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.3.846-849.1984
更新日期:1984-03-01 00:00:00
abstract::Haemophilus ducreyi, the causative agent of chancroid, produces a lipooligosaccharide (LOS) which terminates in N-acetyllactosamine. This glycoform can be further extended by the addition of a single sialic acid residue to the terminal galactose moiety. H. ducreyi does not synthesize sialic acid, which must be acquire...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.10.6727-6735.2005
更新日期:2005-10-01 00:00:00
abstract::Legionnaires' disease is clinically manifested as severe pneumonia caused by Legionella pneumophila. However, the dendritic cell (DC)-centered immunological framework of the host defense against L. pneumophila has not been fully delineated. For this study, we focused on a potent chemoattractant for lymphocytes, fracta...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.9.5350-5357.2005
更新日期:2005-09-01 00:00:00
abstract::Pathogenic Leptospira spp. are likely to encounter higher concentrations of reactive oxygen species induced by the host innate immune response. In this study, we characterized Leptospira interrogans catalase (KatE), the only annotated catalase found within pathogenic Leptospira species, by assessing its role in resist...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00466-12
更新日期:2012-11-01 00:00:00
abstract::Brucella abortus and polyacrylic acid, two agents whose interferon-inducing capacity in the mouse has been well established, failed to stimulate circulating interferon production in rabbits, yet protected rabbits against local vaccinia virus infection. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.8.4.669-673.1973
更新日期:1973-10-01 00:00:00
abstract::There is a relationship between schistosomiasis and anemia, although the magnitude and exact mechanisms involved are unclear. In a cohort of 580 Schistosoma japonicum-infected 7- to 30-year-old patients from Leyte, The Philippines, we evaluated the impact of reinfection with S. japonicum after treatment with praziquan...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00757-06
更新日期:2006-11-01 00:00:00
abstract::It was found that cells of Nocardia asteroides GUH-2 (virulent) were approximately 10 times more virulent than cells of N. asteroides 14759 (intermediate) and greater that 500 times more virulent than N. asteroides 10905 (avirulent) cells when early-stationary-phase cultures suspended in saline were injected intraven...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.3.995-1004.1977
更新日期:1977-06-01 00:00:00
abstract::A total of 133 Listeria monocytogenes isolates were characterized by ribotyping and allelic analysis of the virulence genes hly, actA, and inlA to uncover linkages between independent phylogenetic and specific virulence markers. PCR-restriction fragment length polymorphisms revealed 8 hly, 11 inl4, and 2 actA alleles....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.7.2707-2716.1997
更新日期:1997-07-01 00:00:00