Antigen-coated latex particles as a model system for probing monocyte responses in leprosy.

Abstract:

:To study responses to Mycobacterium leprae antigens, we developed an in vitro model system in which latex particles coated with M. leprae sonic extract (MLSON) antigen were presented to monocytes. Uptake and oxidative response as measured by superoxide production to these antigens were investigated. Phagocytosis of MLSON-coated particles was greater than that of control particles in monocytes from both leprosy patients and controls from leprosy-endemic areas; uptake of MLSON-coated particles was higher in monocytes from lepromatous leprosy patients than in cells from tuberculoid leprosy patients and controls. In both patients and controls, uptake of latex particles coated with leprosy antigens triggered very little reduction of nitroblue tetrazolium although the cells were capable of mounting a respiratory burst. Antigen-coated latex particles can therefore be used as a tool to investigate monocyte responses to M. leprae and individual recombinant antigens.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Hasan RS,Dockrell HM,Jamil S,Chiang TJ,Hussain R

doi

10.1128/IAI.61.9.3724-3729.1993

subject

Has Abstract

pub_date

1993-09-01 00:00:00

pages

3724-9

issue

9

eissn

0019-9567

issn

1098-5522

journal_volume

61

pub_type

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