Abstract:
:A differential-display PCR procedure identified the capsular assembly gene kpsD after Escherichia coli type 1 fimbrial binding to mannose-coated Sepharose beads. Limiting-dilution reverse-transcribed PCRs confirmed down-regulation of the kpsD gene, and Northern blot and lacZ fusion analyses showed down-regulation of the kpsFEDUCS region 1 operon. KpsD protein levels fell, and an agglutination test showed less K capsular antigen on the surface following the bacterial ligand-receptor interaction. These data show that binding of type 1 fimbriae (pili) to d-mannose receptors triggers a cross talk that leads to down-regulation of the capsule assembly region 1 operon in uropathogenic E. coli.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Schwan WR,Beck MT,Hultgren SJ,Pinkner J,Woolever NL,Larson Tdoi
10.1128/IAI.73.2.1226-1231.2005keywords:
subject
Has Abstractpub_date
2005-02-01 00:00:00pages
1226-31issue
2eissn
0019-9567issn
1098-5522pii
73/2/1226journal_volume
73pub_type
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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