Abstract:
:Cytoadherence-linked asexual gene 9 (Clag9), a conserved Plasmodium protein expressed during the asexual blood stages, is involved in the cytoadherence of infected red blood cells (RBCs) to the endothelial lining of blood vessels. Here, we show that Plasmodium falciparum Clag9 (PfClag9) is a component of the PfClag9-RhopH complex that is involved in merozoite binding to human erythrocytes. To characterize PfClag9, we expressed four fragments of PfClag9, encompassing the entire protein. Immunostaining analysis using anti-PfClag9 antibodies showed expression and localization of PfClag9 at the apical end of the merozoites. Mass spectrometric analysis of merozoite extracts after immunoprecipitation using anti-PfClag9 antibody identified P. falciparum rhoptry-associated protein 1 (PfRAP1), PfRAP2, PfRAP3, PfRhopH2, and PfRhopH3 as associated proteins. The identified rhoptry proteins were expressed, and their association with PfClag9 domains was assessed by using protein-protein interaction tools. We further showed that PfClag9 binds human RBCs by interacting with the glycophorin A-band 3 receptor-coreceptor complex. In agreement with its cellular localization, PfClag9 was strongly recognized by antibodies generated during natural infection. Mice immunized with the C-terminal domain of PfClag9 were partially protected against a subsequent challenge infection with Plasmodium berghei, further supporting a biological role of PfClag9 during natural infection. Taken together, these results provide direct evidence for the existence of a PfRhopH-Clag9 complex on the Plasmodium merozoite surface that binds to human RBCs.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Chourasia BK,Deshmukh A,Kaur I,Paul G,Panda A,Rathore S,Singh SK,Theisen M,Mohmmed A,Malhotra Pdoi
10.1128/IAI.00504-19subject
Has Abstractpub_date
2020-01-22 00:00:00issue
2eissn
0019-9567issn
1098-5522pii
IAI.00504-19journal_volume
88pub_type
杂志文章abstract::Strategies to limit complement deposition on Streptococcus pneumoniae are established as virulence features for invasive disease, but their role in respiratory tract infection requires further analysis. We evaluated complement C3 protein deposition on discordant S. pneumoniae isolates of the same serotype (6A) and the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01148-08
更新日期:2009-03-01 00:00:00
abstract::Agglutinogen 1 of Bordetella pertussis strain 353/Z (serotype 1) was separated from protective antigen and histamine-sensitizing factor by starch-block electrophoresis. Most of the agglutinogen 1 migrated towards the cathode in starch-block electrophoresis, although some remained near the origin. Fractions containing ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.3.2.243-248.1971
更新日期:1971-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.4.1334-1341.1998
更新日期:1998-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 临床试验,杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1994-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.2.786-793.1982
更新日期:1982-08-01 00:00:00
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更新日期:2005-04-01 00:00:00
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更新日期:2009-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1990-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.6.1589-1592.1988
更新日期:1988-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.5.1879-1883.1996
更新日期:1996-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2004-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:1999-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.12.5252-5260.1993
更新日期:1993-12-01 00:00:00
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journal_title:Infection and immunity
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更新日期:2007-01-01 00:00:00
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journal_title:Infection and immunity
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更新日期:2005-01-01 00:00:00
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journal_title:Infection and immunity
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更新日期:2006-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.5.1409-1412.1989
更新日期:1989-05-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1977-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.2.442-448.1985
更新日期:1985-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.2.427-433.1979
更新日期:1979-05-01 00:00:00
abstract::3-O-methylmannose isolated from Coccidioides immitis was characterized by gas chromatographic comparison of derivatives with those of synthetic 3-O-methylmannose. Gas chromatographic behavior of three derivatives of the natural and synthetic sugars was identical. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.4.5.660-661.1971
更新日期:1971-11-01 00:00:00
abstract::Rats and mice infected with Trypanosoma lewisi and Trypanosoma musculi, respectively, showed hyperreactivity to lipopolysaccharide (LPS) from gram-negative bacteria. Fatal shock could be precipitated with a dose of LPS 100 to 1,000 times less in infected compared with noninfected animals. In trypanosome-infected rats ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.46.2.501-506.1984
更新日期:1984-11-01 00:00:00
abstract::Burkholderia pseudomallei, a causative agent of melioidosis, is a Gram-negative facultative intracellular bacterium that can survive and multiply in macrophages. Previously, we demonstrated that B. pseudomallei failed to activate gene expression downstream of the MyD88-independent pathway, particularly the expression ...
journal_title:Infection and immunity
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更新日期:2011-07-01 00:00:00
abstract::Minor salivary glands of the oral mucosa in healthy monkeys (Macaca fascicularis and Macaca mulatta) contain organized structural units suitable for recognizing and processing antigens. A previous study of M. fascicularis monkeys provided experimental evidence of retrograde access of oral antigens deep into the minor ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.41.1.399-409.1983
更新日期:1983-07-01 00:00:00
abstract::Type IV pili (Tfp) of gram-negative species share many characteristics, including a common architecture and conserved biogenesis pathway. Much less is known about the regulation of Tfp expression in response to changing environmental conditions. We investigated the diversity of Tfp regulatory systems by searching for ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.2.691-700.2004
更新日期:2004-02-01 00:00:00