Abstract:
:Recent efforts to develop a vaccine against the diarrheal disease cholera have focused on the use of live attenuated strains of the causative organism, Vibrio cholerae. The Ogawa lipopolysaccharide phenotype is expressed by many epidemic strains, and motility defects reduce the risk of reactive diarrhea in vaccine recipients. We therefore converted a motile Inaba(+) vaccine candidate, Peru-2, to a nonmotile Ogawa(+) phenotype using a mariner-based transposon carrying rfbT, the gene required for expression of the Ogawa phenotype. Analysis of 22 nonmotile Peru-2 mutants showed that two were Ogawa(+), and both of these strains had insertions in the flgE gene. It was possible to convert these strains to antibiotic sensitivity by introducing a recombinase that acts on sites flanking the antibiotic marker on the transposon. The resulting strains are competent for colonization in infant mice and may therefore be suitable as vaccine candidates for use either independently or in a combination with strains of different biotypes and serotypes.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Chiang SL,Mekalanos JJdoi
10.1128/iai.68.11.6391-6397.2000keywords:
subject
Has Abstractpub_date
2000-11-01 00:00:00pages
6391-7issue
11eissn
0019-9567issn
1098-5522journal_volume
68pub_type
杂志文章abstract::The role of the alternative sigma factor sigma(B) in Staphylococcus epidermidis was investigated by the construction, complementation, and characterization of a sigB deletion mutant. Electrophoretic analyses confirmed a profound influence of sigma(B) on the expression of exoproteins and cytoplasmic proteins. Detailed ...
journal_title:Infection and immunity
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doi:10.1128/IAI.69.12.7933-7936.2001
更新日期:2001-12-01 00:00:00
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doi:10.1128/IAI.25.1.357-365.1979
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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doi:10.1128/IAI.39.2.520-527.1983
更新日期:1983-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.3.935-939.1982
更新日期:1982-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1992-08-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1995-03-01 00:00:00
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更新日期:1996-12-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.00238-09
更新日期:2009-12-01 00:00:00
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更新日期:1974-05-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.1.144-150.1981
更新日期:1981-01-01 00:00:00
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pub_type: 杂志文章
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journal_title:Infection and immunity
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更新日期:2004-11-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1996-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1999-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.3.1092-1101.1983
更新日期:1983-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1977-06-01 00:00:00