Role of complement in host defense against pneumococcal otitis media.


:Strategies to limit complement deposition on Streptococcus pneumoniae are established as virulence features for invasive disease, but their role in respiratory tract infection requires further analysis. We evaluated complement C3 protein deposition on discordant S. pneumoniae isolates of the same serotype (6A) and their capacity to cause nasopharyngeal (NP) colonization and experimental otitis media (EOM) in an animal model. We compared C3 binding to five 6A isolates from asymptomatic NP carriers with five 6A strains that caused invasive disease, and we observed less C3 ( approximately 10-fold less fluorescence) binding to invasive isolates. We selected two high-level C3-binding carriage and two low-level C3-binding invasive 6A isolates for further study. In the EOM model, 11/12 (92%) ears challenged with a low-level C3-binding 6A strain became infected. Only 2/8 (25%) ears challenged with the discordant high-level C3-binding 6A isolate developed disease (P = 0.005). Results with the second discordant 6A isolate pair were comparable. Cobra venom factor (CoVF) treatment, which depletes C3 and consumes complement, restored virulence of the high-level C3-binding strain; 8/8 (100%) ears in CoVF-treated animals developed EOM compared to only 25% of ears in naïve animals (P = 0.007). These studies demonstrate the critical role for complement evasion in pneumococcal EOM. Colonization with carriage isolates that bound high levels of C3 caused EOM in fewer animals compared to low-level C3-binding invasive strains. Thus, limiting C3 deposition on the surface of S. pneumoniae correlates with increased incidence of EOM following NP colonization and barotrauma in the animal model.


Infect Immun


Infection and immunity


Sabharwal V,Ram S,Figueira M,Park IH,Pelton SI




Has Abstract


2009-03-01 00:00:00














  • Antitoxic immunity in experimental cholera: observations with purified antigens and the rat foot edema model.

    abstract::The recently introduced choleragen-induced rat foot edema model has been employed as a bioassay for evaluating the immunogenicity of three purified preparations containing cholera exo-enterotoxin antigen, choleragen, choleragenoid, and Formalin-treated choleragen (formagen). The results indicated that choleragen evoke...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Finkelstein RA,Hollingsworth RC

    更新日期:1970-05-01 00:00:00

  • Local and systemic antibody responses in mice immunized intranasally with native and detergent-extracted outer membrane vesicles from Neisseria meningitidis.

    abstract::The mouse humoral immune response toward native or detergent-extracted outer membrane vesicles (NOMVs and DOMVs, respectively) from Neisseria meningitidis was determined after intranasal immunization. Both preparations elicited high frequencies of NOMV-specific antibody-forming cells (AFCs) locally in the nasal associ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Guthrie T,Wong SY,Liang B,Hyland L,Hou S,Høiby EA,Andersen SR

    更新日期:2004-05-01 00:00:00

  • Improved tuberculosis DNA vaccines by formulation in cationic lipids.

    abstract::Mice were vaccinated with plasmid DNA (pDNA) encoding antigen 85A (Ag85A), Ag85B, or PstS-3 from Mycobacterium tuberculosis either in saline or formulated for intramuscular injections in VC1052:DPyPE (aminopropyl-dimethyl-myristoleyloxy-propanaminium bromide-diphytanoylphosphatidyl-ethanolamine) (Vaxfectin; Vical, Inc...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: D'Souza S,Rosseels V,Denis O,Tanghe A,De Smet N,Jurion F,Palfliet K,Castiglioni N,Vanonckelen A,Wheeler C,Huygen K

    更新日期:2002-07-01 00:00:00

  • Two copies of Shiga-like toxin II-related genes common in enterohemorrhagic Escherichia coli strains are responsible for the antigenic heterogeneity of the O157:H- strain E32511.

    abstract::Thirty-two clinical isolates of Shiga-like toxin (SLT)-producing Escherichia coli associated with single cases or outbreaks of bloody diarrhea, hemorrhagic colitis, the hemolytic uremic syndrome, or edema disease of swine were examined for multiple copies of genes belonging to the slt-I or slt-II toxin families. Five ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Schmitt CK,McKee ML,O'Brien AD

    更新日期:1991-03-01 00:00:00

  • Moraxella catarrhalis bacterium without endotoxin, a potential vaccine candidate.

    abstract::Lipooligosaccharide (LOS) is a major surface component of Moraxella catarrhalis and a possible virulence factor in the pathogenesis of human infections caused by this organism. The presence of LOS on the bacterium is an obstacle to the development of vaccines derived from whole cells or outer membrane components of th...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Peng D,Hong W,Choudhury BP,Carlson RW,Gu XX

    更新日期:2005-11-01 00:00:00

  • Studies on the effect of starvation on mycobacteria.

    abstract::Ten cultures of Mycobacterium tuberculosis, one of Mycobacterium kansasii (nonsignificant), and one of Mycobacterium phlei were submitted to starvation. As a result they lost first their acid fastness and then all other staining affinities but, in this chromophobic state, they survived for at least 2 years and, after ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Nyka W

    更新日期:1974-05-01 00:00:00

  • Partial purification and characterization of heat-stable enterotoxin produced by Yersinia enterocolitica.

    abstract::By using a suckling mouse assay, heat-stable enterotoxin (ST) was purified from the culture filtrate of Yersinia enterocolitica isolated from a diarrheal patient. The purification procedures involve ultrafiltration with an Amicon HIP-10 hollow fiber, ethanol fractionation, protamine sulfate treatment, diethylaminoethy...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Okamoto K,Inoue T,Ichikawa H,Kawamoto Y,Miyama A

    更新日期:1981-02-01 00:00:00

  • Effect of low- and high-virulence Yersinia enterocolitica strains on the inflammatory response of human umbilical vein endothelial cells.

    abstract::Pathogenic strains of Yersinia spp. inject a set of Yop effector proteins into eukaryotic cells by using a plasmid-encoded type III secretion system. In this study, we analyzed the inflammatory response of human umbilical vein endothelial cells (HUVECs) after infection with different Yersinia enterocolitica strains. W...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Denecker G,Tötemeyer S,Mota LJ,Troisfontaines P,Lambermont I,Youta C,Stainier I,Ackermann M,Cornelis GR

    更新日期:2002-07-01 00:00:00

  • Effect of CpG oligodeoxynucleotides on the immunogenicity of Pfs25, a Plasmodium falciparum transmission-blocking vaccine antigen.

    abstract::Antibodies directed against Pfs25, a protein present on the surface of zygotes and ookinetes of Plasmodium falciparum, completely block pathogen transmission. We evaluated the immunomodulatory effect of CpG oligodeoxynucleotides (ODN) on the immunogenicity of recombinant Pfs25 (rPfs25) formulated in alum (Al). Immuniz...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Coban C,Ishii KJ,Stowers AW,Keister DB,Klinman DM,Kumar N

    更新日期:2004-01-01 00:00:00

  • Heterophil antigen in bovine sera detectable by immune adherence hemagglutination with infectious mononucleosis sera.

    abstract::Bovine sera for tissue culture use were shown to contain heterophil antigen of the Paul-Bunnell-Davidsohn type by immune adherence hemagglutination tests with sera from patients with infectious mononucleosis. The antibody titers observed were comparable to those determined by two other methods; i.e., the immune adhere...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Lennette ET,Henle G,Henle W,Horwitz CA

    更新日期:1978-03-01 00:00:00

  • Localization of the third component of complement on the cell wall of encapsulated Staphylococcus aureus M: implications for the mechanism of resistance to phagocytosis.

    abstract::Encapsulated Staphylococcus aureus strains are more virulent than unencapsulated staphylococci, and this phenomenon has been associated with decreased opsonization of encapsulated bacteria by normal human serum. Peptidoglycan, a major cell wall component of S. aureus, has been shown to promote opsonization of this bac...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Wilkinson BJ,Sisson SP,Kim Y,Peterson PK

    更新日期:1979-12-01 00:00:00

  • CD8 T-cell recognition of macrophages and hepatocytes results in immunity to Listeria monocytogenes.

    abstract::CD8 T cells are effective mediators of specific immunity to infection by Listeria monocytogenes, a bacterial pathogen that initially infects macrophages in the spleen and liver and subsequently spreads to hepatocytes and unidentified parenchymal cells in the spleen. To identify the in vivo target cells of L. monocytog...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Harty JT,Bevan MJ

    更新日期:1996-09-01 00:00:00

  • Role of the HefC efflux pump in Helicobacter pylori cholesterol-dependent resistance to ceragenins and bile salts.

    abstract::The human gastric pathogen Helicobacter pylori modifies host cholesterol via glycosylation and incorporates the glycosylated cholesterol into its membrane; however, the benefits of cholesterol to H. pylori are largely unknown. We speculated that cholesterol in the H. pylori membrane might alter the susceptibility of t...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Trainor EA,Horton KE,Savage PB,Testerman TL,McGee DJ

    更新日期:2011-01-01 00:00:00

  • Type III secretion-dependent modulation of innate immunity as one of multiple factors regulated by Pseudomonas aeruginosa RetS.

    abstract::Mutation of retS (rtsM) of Pseudomonas aeruginosa strain PA103 reduces its virulence in both ocular and respiratory murine models of infection. In vitro, retS mutants exhibit loss of the ExsA-regulated type III secretion system (TTSS), reduced twitching motility, and a decrease in association with, invasion of, and su...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Zolfaghar I,Evans DJ,Ronaghi R,Fleiszig SM

    更新日期:2006-07-01 00:00:00

  • Comparative translocation of enteropathogenic Campylobacter spp. and Escherichia coli from the intestinal tract of gnotobiotic mice.

    abstract::In C3H gnotobiotic mice, no significant difference was observed between the translocation of nine clinical isolates of enteropathogenic Campylobacter spp. and that of seven nonisogeneic Escherichia coli strains with or without various determinants of pathogenicity. In addition, there was no correlation found between t...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Youssef M,Corthier G,Goossens H,Tancrede C,Henry-Amar M,Andremont A

    更新日期:1987-04-01 00:00:00

  • Antigenic polypeptide complex from the Melvin strain of Neisseria gonorrhoeae: isolation and properties.

    abstract::An antigenic complex has been isolated in a highly purified from from the Melvin strain of Neisseria gonorrhoeae. The complex has a molecular weight of 9.3 x 10(6) and on sodium dodecyl sulfate-polyacrylamide gel electrophoresis was found to consist of several subunits; the most predominant had the following molecular...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Karkhanis YD,Anderson RL,Zeltner JY,Carlo DJ,Stoudt TH

    更新日期:1979-08-01 00:00:00

  • Structural and antigenic analysis of Chlamydia pneumoniae.

    abstract::Several isolates of Chlamydia pneumoniae were compared with each other and to Chlamydia trachomatis and Chlamydia psittaci by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblots. Protein profiles of the C. pneumoniae isolates appeared to be the same and were distinct from the other Chlamydia spec...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Campbell LA,Kuo CC,Grayston JT

    更新日期:1990-01-01 00:00:00

  • Kinetics of Bartonella birtlesii infection in experimentally infected mice and pathogenic effect on reproductive functions.

    abstract::The kinetics of infection and the pathogenic effects on the reproductive function of laboratory mice infected with Bartonella birtlesii recovered from an Apodemus species are described. B. birtlesii infection, as determined by bacteremia, occurred in BALB/c mice inoculated intravenously. Inoculation with a low-dose in...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Boulouis HJ,Barrat F,Bermond D,Bernex F,Thibault D,Heller R,Fontaine JJ,Piémont Y,Chomel BB

    更新日期:2001-09-01 00:00:00

  • Antigenic specificity of convalescent serum from cattle with haemophilus somnus-induced experimental abortion.

    abstract::The antigens of Haemophilus somnus recognized by convalescent bovine serum were studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting with a protein A-peroxidase conjugate. The same two 76K and 40K antigens were predominant in whole-bacterium preparations and in outer-membrane-enric...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Corbeil LB,Arthur JE,Widders PR,Smith JW,Barbet AF

    更新日期:1987-06-01 00:00:00

  • Gender is a major factor in determining the severity of mycoplasma respiratory disease in mice.

    abstract::Gender is a significant factor in determining the susceptibility to and severity of pulmonary diseases in both humans and animals. Murine respiratory mycoplasmosis (MRM), due to Mycoplasma pulmonis infection, is an excellent animal model for evaluation of the role of various host factors on the development of acute or...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Yancey AL,Watson HL,Cartner SC,Simecka JW

    更新日期:2001-05-01 00:00:00

  • Immunization with a polyprotein vaccine consisting of the T-Cell antigens thiol-specific antioxidant, Leishmania major stress-inducible protein 1, and Leishmania elongation initiation factor protects against leishmaniasis.

    abstract::Development of an effective vaccine against Leishmania infection is a priority of tropical disease research. We have recently demonstrated protection against Leishmania major in the murine and nonhuman primate models with individual or combinations of purified leishmanial recombinant antigens delivered as plasmid DNA ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Coler RN,Skeiky YA,Bernards K,Greeson K,Carter D,Cornellison CD,Modabber F,Campos-Neto A,Reed SG

    更新日期:2002-08-01 00:00:00

  • Association of Mycoplasma arthritidis mitogen with lethal toxicity but not with arthritis in mice.

    abstract::Mycoplasma arthritidis induces an acute to chronic arthritis in rodents. Arthritis induced in mice histologically resembles human rheumatoid arthritis and can be associated with lethal toxicity following systemic injection. The M. arthritidis mitogen (MAM) superantigen has long been implicated as having a role in path...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Luo W,Yu H,Cao Z,Schoeb TR,Marron M,Dybvig K

    更新日期:2008-11-01 00:00:00

  • Bordetella pertussis risA, but not risS, is required for maximal expression of Bvg-repressed genes.

    abstract::Expression of virulence determinants by Bordetella pertussis, the primary etiological agent of whooping cough, is regulated by the BvgAS two-component regulatory system. The role of a second two-component regulatory system, encoded by risAS, in this process is not defined. Here, we show that mutation of B. pertussis r...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Stenson TH,Allen AG,Al-Meer JA,Maskell D,Peppler MS

    更新日期:2005-09-01 00:00:00

  • Characterization of porcine intestinal receptors for the K88ac fimbrial adhesin of Escherichia coli as mucin-type sialoglycoproteins.

    abstract::We have previously identified two K88ac adhesion receptors (210 and 240 kDa) which are present in membrane preparations from adhesive but not nonadhesive porcine intestinal brush border cells; these adhesin receptors are postulated to be important determinants of the susceptibility of pigs to K88ac+ enterotoxigenic Es...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Erickson AK,Baker DR,Bosworth BT,Casey TA,Benfield DA,Francis DH

    更新日期:1994-12-01 00:00:00

  • Generation and Evaluation of a Glaesserella (Haemophilus) parasuis Capsular Mutant.

    abstract::Glaesserella (Haemophilus) parasuis is a commensal bacterium of the upper respiratory tract in pigs and also the causative agent of Glässer's disease, which causes significant morbidity and mortality in pigs worldwide. Isolates are characterized into 15 serovars by their capsular polysaccharide, which has shown a corr...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Eberle KC,Hau SJ,Luan SL,Weinert LA,Stasko JA,Wang J,Peters SE,Langford PR,Rycroft AN,Wren BW,Maskell DJ,Tucker AW,Brockmeier SL

    更新日期:2020-04-20 00:00:00

  • Synthesis and surface expression of CD14 by human endothelial cells.

    abstract::Previous studies have reported that human vascular endothelial cells lack the membrane-bound lipopolysaccharide (LPS) receptor, CD14 (mCD14). By optimizing assay conditions, including the selection of anti-CD14 monoclonal antibody, we now demonstrate that human umbilical vein endothelial cells (HUVEC) express CD14 on ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Jersmann HP,Hii CS,Hodge GL,Ferrante A

    更新日期:2001-01-01 00:00:00

  • Adenylate cyclase toxin is critical for colonization and pertussis toxin is critical for lethal infection by Bordetella pertussis in infant mice.

    abstract::Proliferation of Bordetella pertussis in the lungs of infant mice challenged by the intranasal route was examined. The bacteria rapidly proliferated in the lungs of mice challenged with a sublethal dose of a wild-type strain (BP338) or a filamentous hemagglutinin mutant (BPM409) from 500 at day 0 to 10(7) at day 15. T...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Goodwin MS,Weiss AA

    更新日期:1990-10-01 00:00:00

  • Gene clusters governing the production of hemolysin and mannose-resistant hemagglutination are closely linked in Escherichia coli serotype O4 and O6 isolates from urinary tract infections.

    abstract::The genes encoding alpha-hemolysin and mannose-resistant hemagglutination were shown to be closely linked in cloned DNA from two Escherichia coli urinary tract isolates of serotypes O4 (J96) and O6 (C1212). DNA hybridization experiments demonstrated that the hly and mrh gene clusters of other E. coli O6 serotypes were...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Low D,David V,Lark D,Schoolnik G,Falkow S

    更新日期:1984-01-01 00:00:00

  • Comparison of plasmid deoxyribonucleic acid contents, culture characteristics, and indices of pathogenicity among selected strains of Vibrio parahaemolyticus.

    abstract::Thirty-one Kanagawa-positive and -negative Vibrio parahaemolyticus isolates from patient stools and seafood were examined for plasmid deoxyribonucleic acid content, culture characteristics, and indices of pathogenicity. No significant correlation was found between plasmid deoxyribonucleic acid contents and indices of ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Twedt RM,Brown DF,Zink DL

    更新日期:1981-07-01 00:00:00

  • Role of gingipains in growth of Porphyromonas gingivalis in the presence of human serum albumin.

    abstract::Porphyromonas gingivalis, a bacterium associated with active chronic periodontitis lesions, produces several proteolytic enzymes that are thought to be involved in host colonization, perturbation of the immune system, and tissue destruction. The aim of the present study was to investigate the contribution of Arg- and ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Grenier D,Imbeault S,Plamondon P,Grenier G,Nakayama K,Mayrand D

    更新日期:2001-08-01 00:00:00