Abstract:
:Bactericidal/permeability-increasing protein (BPI), a human neutrophil granule protein, has been shown to bind lipopolysaccharide (LPS) and neutralize LPS-mediated cytokine production in adherent monocytes and the whole-blood system. In this study we investigated the ability of recombinant human BPI (rBPI) to inhibit LPS-induced vascular endothelial cell (EC) injury and activation. rBPI inhibited significantly both rough and smooth LPS-mediated injury for cultured bovine brain microvessel ECs, as measured by lactic dehydrogenase release, and blocked the LPS-induced interleukin-6 (IL-6) release from human umbilical vein ECs in a dose-dependent manner. BPI was able to inhibit LPS-mediated EC injury or activation whether it was added before or at the same time with LPS, but delaying the time of addition of rBPI resulted only in a partial inhibition. BPI also inhibited LPS-induced tumor necrosis factor alpha, IL-1 beta, and IL-6 release from human whole blood. This inhibition of tumor necrosis factor alpha, IL-1 beta, and IL-6 release from whole blood was maximal when BPI was premixed with LPS before addition to blood and was partial when BPI was added simultaneously with LPS, but no inhibition was observed when the addition of rBPI was delayed for 5 min. These findings suggest that rBPI is a potent inhibitor of LPS-mediated responses in ECs and whole blood and underscore the potential use of BPI in treatment or prevention of endotoxic shock. In contrast, the anti-lipid A monoclonal antibodies HA-1A and E5 were ineffective in inhibiting LPS-mediated EC injury and activation as well as LPS-induced cytokine release in whole blood.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Arditi M,Zhou J,Huang SH,Luckett PM,Marra MN,Kim KSdoi
10.1128/IAI.62.9.3930-3936.1994subject
Has Abstractpub_date
1994-09-01 00:00:00pages
3930-6issue
9eissn
0019-9567issn
1098-5522journal_volume
62pub_type
杂志文章abstract::Oral inoculation of 5-day-old gnotobiotic pigs with Salmonella enterica serovar Typhimurium strain F98 resulted in severe enteritis and invasive disease. Preinoculation 24 h earlier with an avirulent mutant of Salmonella enterica serovar Infantis (1326/28) completely prevented disease for up to 14 days (when the exper...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.4.2182-2191.2003
更新日期:2003-04-01 00:00:00
abstract::Enterotoxigenic Escherichia coli (ETEC) is a leading diarrheagenic bacterial pathogen among travelers and children in resource-limited regions. Adherence to host intestinal cells mediated by ETEC fimbriae is believed to be a critical first step in ETEC pathogenesis. These fimbriae are categorized into related classes ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00246-20
更新日期:2020-10-19 00:00:00
abstract::We determined the heavy (H)- and light (L)-chain variable (V) region nucleotide and translated amino acid sequences of the human immunoglobulin M(kappa) monoclonal antibody (MAb) 5E1, which is specific for the polysaccharide capsule of Escherichia coli K1 and Neisseria meningitidis group B (poly[alpha(2-->8)-N-acetyln...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.5.1776-1786.1994
更新日期:1994-05-01 00:00:00
abstract::Borrelia burgdorferi, the bacterium that causes Lyme disease, has a unique segmented genome consisting of numerous linear and circular plasmids and a linear chromosome. Many of these genetic elements have been found to encode factors critical for B. burgdorferi to complete the infectious cycle. However, several plasmi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00219-13
更新日期:2013-08-01 00:00:00
abstract::Antibodies directed against Pfs25, a protein present on the surface of zygotes and ookinetes of Plasmodium falciparum, completely block pathogen transmission. We evaluated the immunomodulatory effect of CpG oligodeoxynucleotides (ODN) on the immunogenicity of recombinant Pfs25 (rPfs25) formulated in alum (Al). Immuniz...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.1.584-588.2004
更新日期:2004-01-01 00:00:00
abstract::Scrub typhus is a potentially lethal infection that is caused by the obligate intracellular bacterium Orientia tsutsugamushi The roles of Toll-like receptor 2 (TLR2) and TLR4 in innate recognition of O. tsutsugamushi have not been elucidated. By overexpression of TLR2 or TLR4 in HEK293 cells, we demonstrated that TLR2...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00185-16
更新日期:2016-11-18 00:00:00
abstract::The suspected correlation between cessation of type C virus production and halt in immunoglobulin secretion by murine myeloma cells was studied. Employing two variants of the murine myeloma MOPC-315, immunoglobulin A-producing and nonproducing cells, we demonstrated that the two myelomas release similar levels of type...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.25.2.569-573.1979
更新日期:1979-08-01 00:00:00
abstract::Humans are the sole reservoir of Neisseria gonorrhoeae, an organism which undergoes a marked increase in metabolic rate after exposure to a low-molecular-weight, heat-stable component(s) of human serum. Further studies on the effect of serum on gonococcal metabolism were undertaken. Gonococcal broth (GCB) is commonly ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.3.738-744.1985
更新日期:1985-12-01 00:00:00
abstract::Lack of efficacious vaccines against Mycobacterium tuberculosis (MTB) infection is a limiting factor in the prevention and control of tuberculosis (TB), the leading cause of death from an infectious agent. Improvement or replacement of the BCG vaccine with one that reliably protects all age-groups is urgent. Concerns ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00585-20
更新日期:2020-12-14 00:00:00
abstract::The objective of this study was to determine (i) if complementation of ureB-negative Helicobacter pylori restores colonization and (ii) if urease is a useful reporter for promoter activity in vivo. Strains used were M6, M6DeltaureB, and 10 recombinant derivatives of M6 or M6DeltaureB in which urease expression was und...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.2.771-778.2002
更新日期:2002-02-01 00:00:00
abstract::A simple and convenient method was developed for the preparation of Streptococcus pneumoniae type 14 polysaccharide (Pn14PS)-tetanus toxoid (TT) conjugate vaccines, using terminally linked Pn14PS fragments of different lengths. Native Pn14PS was simultaneously depolymerized and activated for conjugation by partial N-d...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.6.2441-2446.1998
更新日期:1998-06-01 00:00:00
abstract::We examined the intracellular growth of 20 strains within six species of Legionella in thioglycolate-elicited peritoneal macrophages from A/J and C57BL/6 mice and a congenic strain derived from them (A.B Lgn1). With the exception of Legionella pneumophila Togus-1 and Bloomington-2, the intracellular growth of the 15 L...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.5.1842-1845.1996
更新日期:1996-05-01 00:00:00
abstract::A peptidoglycan layer of Treponema pallidum kazan was isolated by solubilization of whole cells with 1% warm sodium dodecyl sulfate and subsequent digestion of an insoluble residue with proteases. Electron microscopy revealed that the peptidoglycan was isolated as a single-layered sacculus of less than 5 nm in thickne...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.2.767-774.1981
更新日期:1981-02-01 00:00:00
abstract::Ecto-5'-nucleotidase (CD73) is expressed abundantly on the apical surface of intestinal epithelial cells (IECs) and functions as the terminal enzyme in the generation of extracellular adenosine. Previous work demonstrated that adenosine signaling in IECs results in a number of tissue-protective effects during inflamma...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01022-16
更新日期:2017-09-20 00:00:00
abstract::Anthrax lethal toxin (LeTx), consisting of protective antigen (PA) and lethal factor (LF), rapidly kills primary mouse macrophages and macrophage-like cell lines such as RAW 264.7. LF is translocated by PA into the cytosol of target cells, where it acts as a metalloprotease to cleave mitogen-activated protein kinase k...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.6.3055-3060.1999
更新日期:1999-06-01 00:00:00
abstract::Staphylococcus aureus Newman with an insertion mutation in clfB, the gene encoding clumping factor B, only marginally decreased infection rate (P>0.05) in rats with experimental endocarditis. In contrast, clfB complementation on a multicopy plasmid significantly increased infectivity (P<0.05) over the deleted mutants....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.9.5443-5446.2000
更新日期:2000-09-01 00:00:00
abstract::The mechanism of enhanced resistance of Mycobacterium bovis BCG-treated mice to ectromelia virus infection was investigated by determining the effect of splenectomy, antithymocyte serum, and antimacrophage serum on resistance. It was greatly reduced by these treatments, not only in normal mice, but also in mice treate...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.2.567-573.1983
更新日期:1983-11-01 00:00:00
abstract::Culture supernatants from antigen-pulsed spleen cells of mice infected previously with either BCG or Plasmodium chabaudi were used to study macrophage activation as judged by phagocytosis of immunoglobulin G-sensitized erythrocytes and Plasmodium berghei- and P. chabaudi-infected erythrocytes. Resident peritoneal macr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.2.733-739.1983
更新日期:1983-11-01 00:00:00
abstract::The innate immune system recognizes pathogen-associated molecular patterns (PAMPs) through pattern recognition receptors (PRR) and transduces downstream signaling to activate the host defense. Here we report that in addition to direct PAMP-PRR interactions, live Candida albicans cells can release soluble factors to ac...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01041-09
更新日期:2010-01-01 00:00:00
abstract::Studies of the effect of sex hormones on the susceptibility of mice to the disseminated gonococcal infection demonstrated significantly enhanced susceptibility of mice injected with estrogen (17 beta-estradiol). In mice treated with estradiol, bacteremia progressively developed within 12 h postinoculation and mice die...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.49.1.238-243.1985
更新日期:1985-07-01 00:00:00
abstract::The formation of water-insoluble glucan by extracellular glucosyltransferase from Streptococcus mutans 6715 found to be greatly stimulated by various mono- or divalent cations. An enzyme preparation, obtained by ethanol fractionation, was able to catalyze the formation of water-insoluble glucan from sucrose in the pre...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.23.3.564-570.1979
更新日期:1979-03-01 00:00:00
abstract::We examined 205 enterotoxigenic strains of Escherichia coli for colonization factor antigens (CFA) I and II, using an immunodiffusion technique with specific antisera. A total of 36 strains of serogroups O63, O78, O114, O128, and O153 and 1 rough strain possessed CFA/I and gave a single precipitin line; 47 strains of ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.36.1.189-197.1982
更新日期:1982-04-01 00:00:00
abstract::The opportunistic pathogen Pseudomonas aeruginosa is a major cause of sepsis in severely burned patients. If it is not eradicated from the wound, it translocates to the bloodstream, causing sepsis, multiorgan failure, and death. We recently described the P. aeruginosa heparinase-encoding gene, hepP, whose expression w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00755-17
更新日期:2017-12-19 00:00:00
abstract::Toxoplasma gondii and Neospora caninum (both Apicomplexa) are closely related cyst-forming coccidian parasites that differ significantly in their host ranges and ability to cause disease. Unlike eutherian mammals, Australian marsupials (metatherian mammals) have long been thought to be highly susceptible to toxoplasmo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00173-17
更新日期:2017-05-23 00:00:00
abstract::The relationship of catalase, cystinase, and deoxyribonuclease activity to toxinogeny in Corynebacterium diphtheriae was examined. Mutants deficient in each activity were isolated after mutagenization of strain C4 with nitrosoguanidine. All mutants were converted to toxinogeny after lysogenization with beta-converting...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.8.3.442-445.1973
更新日期:1973-09-01 00:00:00
abstract::Thirty two anaerobic bacterial strains were screened for their in vitro inhibitory activity toward 21 Neisseria gonorrhoeae strains, including two penicillin-resistant strains. From this screening, six anaerobic bacteria belonging to the genera Bacteroides, Peptostreptococcus, and Eubacterium were selected for their l...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-06-01 00:00:00
abstract::We have investigated the roles of gamma interferon (IFN-gamma) and interleukin-4 (IL-4) in host defense against Brugia malayi. Our data suggest that the lack of either IFN-gamma or IL-4 prolongs the time required to achieve sterile immunity, suggesting that both canonical type 1 and type 2 responses are involved in th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.5.3034-3035.2000
更新日期:2000-05-01 00:00:00
abstract::Stenotrophomonas maltophilia is a ubiquitous bacterium and an emerging nosocomial pathogen. This bacterium is resistant to many antibiotics, associated with a number of infections, and a significant health risk, especially for immunocompromised patients. Given that Caenorhabditis elegans shares many conserved genetic ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00711-15
更新日期:2015-12-07 00:00:00
abstract::Phagocytosis, H2O2 production, Mac-1 expression, and in vivo elimination of Listeria monocytogenes were enhanced in granulocyte colony-stimulating factor (G-CSF)-treated mice. Transfer of polymorphonuclear leukocytes prolonged survival of mice infected with a lethal dose of L. monocytogenes. G-CSF augments the functio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.12.4740-4743.1991
更新日期:1991-12-01 00:00:00
abstract::Adherence to fibrinogen and fibronectin plays a crucial role in Staphylococcus aureus experimental endocarditis. Previous genetic studies have shown that infection and carriage isolates do not systematically differ in their virulence-related genes, including genes conferring adherence, such as clfA and fnbA. We set ou...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01274-09
更新日期:2010-04-01 00:00:00