Abstract:
:Subtilase cytotoxin (SubAB) is a recently identified AB5 subunit toxin produced by Shiga-toxigenic Escherichia coli. The A subunit is thought to be a subtilase-like, serine protease, whereas the B subunit binds to the toxin receptor on the cell surface. We cloned the genes from a clinical isolate; the toxin was produced as His-tagged proteins. SubAB induced vacuolation at concentrations greater than 1 microg/ml after 8 h, in addition to the reported cytotoxicity induced at a ng/ml level after 48 h. Vacuolation was induced with the B, but not the A, subunit and was dependent on V-type ATPase. The cytotoxicity of SubAB at low concentrations was associated with the inhibition of protein synthesis; the 50% inhibitory dose was approximately 1 ng/ml. The A subunit, containing serine 272, which is thought to be a part of the catalytic triad of a subtilase-like serine protease, plus the B subunit was necessary for this activity, both in vivo and in vitro. SubAB did not cleave azocasein, bovine serum albumin, ovalbumin, or synthetic peptides. These data suggest that SubAB is a unique AB toxin: first, the B subunit alone can induce vacuolation; second, the A subunit containing serine 272 plus the B subunit inhibited protein synthesis, both in vivo and in vitro; and third, the A subunit proteolytic activity may have a strict range of substrate specificity.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Morinaga N,Yahiro K,Matsuura G,Watanabe M,Nomura F,Moss J,Noda Mdoi
10.1128/IAI.01336-06subject
Has Abstractpub_date
2007-01-01 00:00:00pages
488-96issue
1eissn
0019-9567issn
1098-5522pii
IAI.01336-06journal_volume
75pub_type
杂志文章abstract::A novel immunobiologically active fraction was prepared from a phenol-water extract of Prevotella intermedia ATCC 25611 by Sephadex G-100 column chromatography. The fraction consisted mainly of carbohydrate and protein and was devoid of fatty acid. The fraction showed high-molecular-weight bands (10,000 to 12,000) on ...
journal_title:Infection and immunity
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doi:10.1128/IAI.65.11.4531-4538.1997
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journal_title:Infection and immunity
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doi:10.1128/IAI.61.12.5361-5373.1993
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.2.427-433.1979
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.46.3.867-869.1984
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.11.4793-4800.1992
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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doi:
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1994-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1991-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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