Abstract:
:Sephadex G-200 filtration was used for fractionating dengue type 2 (DEN-2)-infected suckling mouse brain (SMB) supernatant fluids. The high-molecular-weight fraction, which was eluted in the void volume, showed no increase in type specificity when tested against immune ascitic fluid to the four prototype dengue viruses. A void-volume fraction obtained after the infected SMB supernatant fluids were treated with urea displayed significant increases in complement fixation (CF) type specificity. Immune ascitic fluid prepared against the more typespecific DEN-2 antigen demonstrated neutralizing ability and greater CF type specificity. When DEN-2 sucrose-acetone-extracted hemagglutinating (HA) antigens were treated with 6 m urea at 37 C for various time intervals, all HA antigen was destroyed in 15 min. Urea treatment of infected SMB supernatant fluids indicated that the CF antigens were more stable to the effect of urea than were the HA antigens. After urea treatment of the SMB supernatant fluids, the CF type specificity increased as the hemagglutination inhibition titer decreased.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Cornesky RA,Hammon WM,Atchison RW,Sather GEdoi
10.1128/IAI.6.6.952-957.1972subject
Has Abstractpub_date
1972-12-01 00:00:00pages
952-7issue
6eissn
0019-9567issn
1098-5522journal_volume
6pub_type
杂志文章abstract::Previous studies have suggested that the Pseudomonas aeruginosa PAK pilus adhesin moiety resides in an epithelial cell-binding domain located in the C-terminal region of the PAK pilin structural protein. Synthetic peptides Ac17red (a synthetic peptide with a sequence identical to that of PAK pilin residues 128 to 144,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.12.3720-3726.1989
更新日期:1989-12-01 00:00:00
abstract::Toxoplasma gondii is an obligate intracellular parasite that resides in the cytoplasm of its host in a unique membrane-bound vacuole known as the parasitophorous vacuole (PV). The membrane surrounding the parasite is remodeled by the dense granules, secretory organelles that release an array of proteins into the vacuo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00782-08
更新日期:2008-11-01 00:00:00
abstract::Pertussis toxin is secreted from Bordetella pertussis with the assistance of the Ptl transport system, a member of the type IV family of macromolecular transporters. The S1 subunit and the B oligomer combine to form the holotoxin prior to export from the bacterial cell, although the site of assembly is not known. To b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.3.1193-1201.2002
更新日期:2002-03-01 00:00:00
abstract::In this work we immunized mice with DNA encoding full-length Tc52 or its amino- or carboxy-terminal (N- and C-term, respectively) domain carried by attenuated Salmonella as a DNA delivery system. As expected, Salmonella-mediated DNA delivery resulted in low antibody titers and a predominantly Th1 response, as shown by...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02190-14
更新日期:2014-10-01 00:00:00
abstract::Yersinia pestis KIM5 was found to be cytotoxic for the IC21 and P388D1 mouse macrophage cell lines, as well as for resident peritoneal macrophages from C57BL/6 mice. Affected cells phagocytosed KIM5 inefficiently, became spherical, detached readily from culture dishes, and retained 51Cr poorly. The cytotoxic effect wa...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.51.3.788-794.1986
更新日期:1986-03-01 00:00:00
abstract::To investigate the correlation between the incA I47T mutation in Chlamydia trachomatis and the nonfusogenic phenotype, the incA genes of 25 isolates were sequenced. Four major sequence types were identified. Seven isolates (28%) had the I47T mutation. Isolates representing the four sequence types expressed IncA in the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.7.4654-4656.2001
更新日期:2001-07-01 00:00:00
abstract::Lipopolysaccharide (LPS) from three human and four monkey isolates of Campylobacter jejuni was extracted in high yields that revealed ladder-like structures in polyacrylamide gels by direct silver staining. These observations demonstrate that C. jejuni possesses LPS with O-chain repeating units typical of the family E...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.12.5384-5387.1993
更新日期:1993-12-01 00:00:00
abstract::One-day-old chicks were infected orally with two strains of weakly hemolytic spirochetes isolated from a human and a pig with intestinal spirochetosis. These spirochetosis both colonized birds, attached end-on to their cecal enterocytes, induced watery diarrhea, and significantly depressed growth rates. Cultures of Se...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.9.3705-3710.1995
更新日期:1995-09-01 00:00:00
abstract::Whooping cough results from infection of the respiratory tract with Bordetella pertussis, and the secreted adenylate cyclase toxin (ACT) is essential for the bacterium to establish infection. Despite extensive study of the mechanism of ACT cytotoxicity and its effects over a range of concentrations in vitro, ACT has n...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00110-13
更新日期:2013-05-01 00:00:00
abstract::Endogenous interleukin-12 (IL-12) mediates protection against Yersinia enterocolitica in C57BL/6 mice by triggering gamma interferon (IFN-gamma) production in NK and CD4+ T cells. Administration of exogenous IL-12 confers protection against yersiniae in Yersinia-susceptible BALB/c mice but exacerbates yersiniosis in r...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.5.2213-2220.1998
更新日期:1998-05-01 00:00:00
abstract::Pathogenicities of 10 temperature-sensitive mutants of Venezuelan encephalitis virus were studied using the hamster model of human virulence. The parental strain and nine of the temperature-sensitive mutants produced lethal infections in hamsters. Strain ts 126 showed reduced hamster virulence. Deaths with the lethal ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.25.3.873-879.1979
更新日期:1979-09-01 00:00:00
abstract::An inactivated oral enterotoxigenic Escherichia coli (ETEC) vaccine against ETEC diarrhea was given to 25 adult Swedish volunteers. The vaccine consisted of formalin-killed E. coli bacteria expressing the most common colonization factor antigens (CFAs), i.e., CFA/I, -II, and -IV, and recombinantly produced cholera B s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.7.3311-3316.1998
更新日期:1998-07-01 00:00:00
abstract::ExoU is a potent effector protein that causes rapid host cell death upon injection by the type III secretion system of Pseudomonas aeruginosa. The N-terminal half of ExoU contains a patatin-like phospholipase A(2) (PLA(2)) domain that requires the host cell cofactor superoxide dismutase 1 (SOD1) for activation, while ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00223-10
更新日期:2010-08-01 00:00:00
abstract::A monoclonal immunoglobulin M antibody, HP15/36, was produced by a hybridoma cell line prepared by fusion of mouse myeloma cell line Sp2/O with spleen cells of mice immunized with Helicobacter pylori D273 (French strain). Immunoelectron microscopy of whole bacteria and ultrathin sections showed that the determinant wa...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.6.2732-2736.1993
更新日期:1993-06-01 00:00:00
abstract::The intracellular protozoan Toxoplasma gondii is well known for its skill at invading and living within host cells. New discoveries are now also revealing the astounding ability of the parasite to inject effector proteins into the cytoplasm to seize control of the host cell. This review summarizes recent advances in o...
journal_title:Infection and immunity
pub_type: 杂志文章,评审
doi:10.1128/IAI.05974-11
更新日期:2012-02-01 00:00:00
abstract::We previously showed the requirement of both T cells and gamma interferon (IFN-gamma)-producing non-T cells for the genetic resistance of BALB/c mice to the development of toxoplasmic encephalitis (TE). In order to define the role of IFN-gamma production and the perforin-mediated cytotoxicity of T cells in this resist...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.8.4432-4438.2004
更新日期:2004-08-01 00:00:00
abstract::A2G mice are genetically resistant to lethal infection with neurotropic and pneumotropic influenza viruses. A possible immunological explanation for this resistance was sought by assessing the effect of cyclophosphamide and X irradiation immunosuppression on the infection of A2G mice with lethal doses of neurovirulent...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.3.576-587.1975
更新日期:1975-03-01 00:00:00
abstract::Enteropathogenic strains of Yersinia enterocolitica harbor a virulence plasmid (70 kilobases) which specifies, at 37 degrees C, a calcium requirement for growth, autoagglutinability, resistance to the bactericidal activity of human serum, and the expression of some outer membrane proteins (OMPs). To map the genes enco...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.48.3.782-786.1985
更新日期:1985-06-01 00:00:00
abstract::The effect of ingestion of heat-killed Histoplasma capsulatum yeast cells on the metabolism of arachidonic acid (20:4) to prostenoids and leukotrienes was examined in murine peritoneal macrophages (M phi s). H. capsulatum-containing M phi s exhibited a metabolite profile similar to that of zymosan-challenged phagocyte...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.7.2559-2564.1992
更新日期:1992-07-01 00:00:00
abstract::Two fragments with molecular weights of 111,000 (fragment I) and 59,000 (fragment II) were separated from each other by gel filtration of dithiothreitol and urea-treated, trypsinized derivative toxin (molecular weight, 170,000) of the proteolytic Okra strain of Clostridium botulinum type B on a column of Sephadex G-20...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.18.3.761-766.1977
更新日期:1977-12-01 00:00:00
abstract::The she gene of Shigella flexneri 2a, which also harbors the internal enterotoxin genes set1A and set1B (F. R. Noriega, GenBank accession no. U35656, 1995) encodes a homolog of the virulence-related immunoglobulin A (IgA) protease-like family of secreted proteins, Tsh, EspC, SepA, and Hap, from an avian pathogenic Esc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.11.4606-4614.1997
更新日期:1997-11-01 00:00:00
abstract::A total of five bacteriological samples from the periodontal pockets of two sisters with localized juvenile periodontitis and their mother with advanced periodontitis was studied. Gram-negative anaerobic rods were predominant in the samples. Bacteroides intermedius and Bacteroides loescheii were the most predominant s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.1.118-121.1984
更新日期:1984-07-01 00:00:00
abstract::Rabbits were immunized with the synthetic peptide EENVEHDA or (EENV)2, corresponding to a tandemly repeated sequence in the C-terminal part of the Plasmodium falciparum antigen Pf155/RESA, or with Escherichia coli-derived fusion proteins containing the corresponding repeats. For all sera, the capacity of the total imm...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.9.2815-2820.1990
更新日期:1990-09-01 00:00:00
abstract::An immunogenic aminopeptidase was purified from Brucella melitensis strain VTRM1. The purification procedure consisted of ammonium sulfate fractionation and three chromatographic steps. This procedure resulted in a yield of 29% and a 144-fold increase in specific activity. The aminopeptidase appeared to be a monomeric...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.9.5238-5244.2003
更新日期:2003-09-01 00:00:00
abstract::Polymorphoneutrophils (PMNs) are important effector cells in host defense against pneumonia. However, PMNs can also induce inflammation and tissue damage. To investigate the contribution of PMNs to host defense against pneumococcal pneumonia, we determined the effect of the PMN-depleting rat monoclonal antibody RB6-8C...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01579-06
更新日期:2007-04-01 00:00:00
abstract::In attempts to reactivate latent herpes simplex virus, we instilled Diplococcus pneumoniae intratracheally into mice harboring latent infections in sacrosciatic spinal ganglia. All mice developed a severe pneumonia within 24 h and were given penicillin therapy. Representative mice that survived the penumonia were sacr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.4.635-639.1975
更新日期:1975-04-01 00:00:00
abstract::When tested for their activity in suppressing the acquisition of immunity during acute Besnoitia infection of hamsters, antilymphocyte serum (ALS), aminopterin, cyclophosphamide, cortisol, and whole-body irradiation were the most active agents and effectively blocked the development of immunity during 4 to 12 days of ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.3.6.756-761.1971
更新日期:1971-06-01 00:00:00
abstract::Neisseria gonorrhoeae is generally considered to be an obligate aerobe; it can, however, grow in the absence of oxygen by anaerobic respiration by using nitrite as a terminal electron acceptor. The outer membrane protein compositions of aerobically and anaerobically grown N. gonorrhoeae strains were compared by one- a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.6.1359-1364.1987
更新日期:1987-06-01 00:00:00
abstract::Borrelia burgdorferi, the causative agent of Lyme disease, loses its ability to infect and cause disease in mammalian hosts after repeated in vitro passage. To identify proteins preferentially expressed by the low-passage strain and thus representing potential virulence factors, the polypeptide profiles of virulent, l...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.11.4662-4672.1992
更新日期:1992-11-01 00:00:00
abstract::Rabbit endogenous pyrogens were of about the same molecular size, but showed considerable heterogeneity of their isoelectric points. We attempted to show that this heterogeneity was attributable to variable glycosylation of a single polypeptide chain. When peritoneal exudate cells were stimulated to make pyrogens in t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.1.184-191.1981
更新日期:1981-10-01 00:00:00